How to correctly analyze raw sequence files of bacterial 16s rRNA partial gene sequence

[@SugandhaAachhera]

Thank you so much for making this fantastic video. I have watched 10 videos by after this video all doubts become clear. if it is possible, please make some video on shotgun whole genome sequencing of bacteria.

[@pulikkanjoby5435]

Great teaching. Thank you

[@drmrunalinibr2869]

Extremely good explanation

[@DweipayanG]

Thank you !!

[@victoriaogor4525]

Very useful sir, thanks.

[@pankajbarfal4784]

thank you very much sir, very informative.

[@bjjjallowjallow7844]

how did you get the chromatogram in bioedit. i am only getting the sequence in bioedit and did not know how you opened both the bioedit and chromatogram to delete uncorrect nucleotides.

[@ahmedkhater5369]

Thank you for your help 🙏

[@anjalighosh13]

Sir forward primer always binds with anti sense strand and reverse primer binds with sense strand.

[@Planetearth0316]

sir the lesson is useful. can u also make a video on "how to align our trimmed consensus 16s rRNA sequence with BLAST hit sequences, including some whole genome sequences hits in MEGA software". Thank you

[@kristinacasandrapava9252]

Hi, what do you do when there are gaps between the sequences after aligning them?

[@repallyayyanna7849]

thanks, its useful video

[@DomiiQuezada]

muchas gracias ❤

[@noorlutphyali7495]

The quary length in this example is 403 , i used the same step and i get 665, please i want to ask is this query length acceptable to be be submitted to NCBI to register the bacteria ?

[@khanhammaadabdulwahab2000]

From where we can download forward and reverse sequences?

[@mekpath5818]

Thank you so much

[@kajalpanchal8239]

Hello sir, does forward mean 5 prime to 3 prime?

[@DweipayanG]

Forward mean , the amplicon that u get by extension of forward primer

[@endalineaniefuna9304]

Thanks a lot!

[@SugandhaAachhera]

please give the website link of bioedit software, bcz there is more than 3-4.

[@thehippocampus9130]

How about for 4peaks?

[@Zahid.Mumtaz]

Please guide me, I am stuck as my consensus sequence is coming in this form. what to do? Example >Consensus -------------------------------------------CTGCCAGTAAGACAGGGATAACGCCCGGAAAC-GAAGCAAATAACGCATA--AACCACTCCGCCAGGGGAG-ACGATCGAAAGACGGAAACGCAAAGCAATT---GACGGGCCCCCGCA-CAAGAGCTAGAGCATGAGGTA-AAGGCGAACCAACGCAAAGAACCATACCAGACCTG-GACA-TCATCGGACA-ACT----CT-AGACCAAAAAACAAGGCTCAACCC-GGAGGGTAAGCGGAAACTCGCCAGCCCGAAA-CACCCAGATAATCC-------------------

[@gnanaraj179]

your diagram is wrong. The forward primer will bind to the second strand, while the reverse primer will bind to the first strand.

[@rupahalder6723]

Sir i have some problems to solving Blast. Can i contact with you through mail or contact number ?