How to establish a Relative Response Factor (RRF)?

Relative Response Factor (RRF) is a critical analytical parameter widely used in chromatographic procedures to quantify impurities in drug substances and dosage products. This video describes the estimation of RRF.

[@mohsentalebi9505]

Keep up the good job. Your videos make the SOPs and ICH guidelines easier to understand.

[@manishpandey7112]

Nice explanation and more informative. Thanks

[@SearchforSomething]

Thanks a lot sir for your valuable and informative video.

[@venkat5098]

Thank you so much sir . good explanation easy to understanding thank you sir.

[@tamngoc9394]

Thanks so much, your presentation is fantastic

[@amitnarayankar]

Can you please make a video on what is recovery and spiking concept in analytics. Thanks

[@manusriRaaz2325]

thankin you sir for making very usefull videos like these ....

[@cbsingh-yn1bn]

Very helpful video sir

[@shara603]

Thanks for making this video sir,,Much needed one.please make video on hplc content method development.

[@naceranaci4139]

Thank you very much

[@suyoggosavi117]

In calculation formula for known impurities why taken 1/RRF.... Why not take only RRF

[@rajilakshmi5133]

When do we calculate RRF? Is there any criteria? In area normalization method can we calculate RTF?

[@prasannakumar1614]

I have confusion on preparation of solutions ,

[@janhavivartak9306]

Thank you sir for providing us with lot of interesting stuff. I had a question in RRF. How do you decide the concentration (ppm) of impurities and API before plotting the linearity graph? Please explain. Thank you.

[@pharmagrowthhub3083]

conc of an impurity and API which lies in the linear range

[@AliRaza-wp8tr]

Sir if u are selecting linear range concentration than why slope is not coming equal to 1

[@rajeshgoud1274]

Considering the Specification of Known impurities and unknown impurities and established LOQ concentration, Linearity will be performed to establish RRF

[@AliRaza-wp8tr]

I have two question plz answer Is impurity is running on different HPLC and is it running on lambda max of impurity Why impurities not shows linear response as API do

[@gkiran3504]

Hello sir Present iam doing RRF for one of my project. But didn't get correct RRF. Then what can i do.. please give me advise...

[@zankarshah6834]

If limit of unknown impurity and known impurity different at that time linearity and range is also different. So why it is necessary to use same Conc. Level for determination RRF by Slope Method??? Can I use different level of impurity and API as per linearity of respective impurity???

[@vangalathirupathireddy6930]

Tnku sir ji

[@MrKrish1029]

We should cover specifications range concentration for impurities then how can we prepare both sample and standard with same concentration This is my doubt sir

[@ask5755]

Why the concentration should be same ?

[@kalavalakhageshwarrao5370]

Sir concentration in PPM or mg/mL

[@fooddelight4057]

You must use chrmatogram to demonstrate it would be more effective

[@suryaprakashrai3263]

Hello sir, thanks for the video. I have a doubt regarding the consideration of salt and base form. If my API is in salt form then should I need to use factor of base and salt for RRF calculation of impurities?

[@rajeshgoud1274]

Hi brother, If your analyte is in Salt form, convert to base form using correction factor, then using your base analyte concentration, establish RRF of impurities

[@sureshkandagatla6126]

kindly show how to calculate % recovery using RRF

[@hemantchauhan1219]

Sir, The Impurity A and API Stock are taken 20 mg and diluted to 100 ml respectively. Than how their concentration is 193.4 and 199 ppm respectively??. Please explain in detail sir.

[@m4madhubabu759]

You have to consider potency of respective imp and API... I.e 20/100*96.7/100*1000=193.4

[@tamngoc9394]

Their potency are different. You have to take potency into account for accurate concentration.

[@sagardhimdhime6771]

How calculate conc.of imp.A..

[@muddy_slipper]

What is API?

[@sagardhimdhime6771]

20mg diluted 100ml that is 200ppm ...so

[@prasannakumar1614]

Potency works

[@sagardhimdhime6771]

193.4 ppm where's come

[@tamngoc9394]

the potency is different