HOW TO IMPROVE CRISPR SPECIFICITY - GENE EDITING EXPLAINED!

[@lynxone7039]

Excellent video - thanks so much for posting this!

[@GenomicsGurus]

Glad you found it useful!

[@mikewilmer8617]

Many thanks! Will be usefull for review writing.

[@edyoucate1774]

Wow nice video, thanks for helping me understand this topic

[@GenomicsGurus]

Glad to hear that

[@lynxone7039]

Excellent video - thanks very much for posting this! One question - what you are calling the 'protospacer' is really that portion of the 'non-target strand' of DNA opposite the 'target strand', the strand that the guide RNA 'spacer' binds to by complementary base-pairing. Protospacers are pieces of viral DNA that still have the PAM attached, and after the PAM is removed the 'spacers' are stored between the repeats, and a spacer and part of the repeat is transcribed into crRNA. So would it be more accurate to label your diagram 'non-target strand' rather than 'protospacer'? I know this is confusing, but apparently that is the terminology used these days?

[@GenomicsGurus]

“Protospacer’, ‘spacer’ and ‘target sequence’ are all terms that have been used generally when describing the first 20 bases of a sgRNA. I don’t disagree with you, but they all work if users know what those bases achieve.

[@lynxone7039]

@@GenomicsGurus Thanks for the quick reply!

[@jedn6488]

Is the tail to tail guide orientation more efficient than prime editing?

[@GenomicsGurus]

We haven't compared the two. Primer editing should give much fewer unwanted edits, which is a big advantage in some applications