How to Make Agar Media and Pour Petri Dishes for Mushroom Cultivation

[@J155P]

This dude is the new, cooler Bill Nye the Science Guy. I'm a big fan.

[@Xulzen]

This is excellent content for getting youth involved in science of mycology, like Bill Nye did for science generally when I was a kid.

[@mindmelt889]

Hes Bill Nye's nephew

[@kristofdonatkovacs8583]

No words to express how much your videos are exactly the perfect content for an absolute beginner and also for someone who has been working with mushrooms for years. Thank you for your enthusiastic content.

[@junglistpostman]

I agree! Paul’s enthusiasm is fantastic and he’s in fact an excellent teacher.

[@joshdeberry7769]

I agree 100%. I fkg love this guy and his videos. Got my young kids (5 &6) to start watching with me and they love the silliness and the characters he does lol

[@user-2df90a0z1]

This is a criminally underrated channel! I love it when our dude does the pirate and other character impressions like the professor. lol

[@robertdrew3864]

This is easily one of the best agar videos on YT. Great explanation, entertaining, and very useful tips.

[@wesleykampe8168]

😂this dude is what I would say should be the face of the myco community… he’s not afraid to let his weird shine through and he understands the pain and the hurdles of starting out as a young mycologist dude I started crying when you said shoplifting flats of mason jars😂😂😂 you are a legend and when I die I want you to know that it may not be a lot but I have taken my wife out of my will and replaced her name with you…fr

[@gabrielestremera9626]

I've been watching these over and over waiting for the next one. It's like rewatching that movie you just can't get enough of! The dude is authentic, knowledgeable, and a true genuine teacher! The humor is just right. My kids' favorite is the pirate character.

[@audreycotten1638]

Excellent timing! Thank you for your generosity- I ordered 1 starter syringe of oyster & received 3 extras!!! Wee! You are awesome! Thank you thank you! 🙏🏻 I have been slightly intimidated by the agar process but have my Petri dishes standing by & hopefully after your video will gain the confidence to begin. Mush Appreciation for your work & beautiful Spirit, Paul! ❤️❤️❤️❤️❤️❤️❤️❤️❤️

[@gustavorodrigues6882]

looking forward for your diy laminar flow hood video. its really expensive to ship your stuff to EU. love the videos tho, keep it up!

[@calebkotewa7344]

Dude has character and extremely useful information I have not found elsewhere. AAAARGGGHHHHH

[@TheTubejunky]

You mean like a SpongeBob knock off pirate? "Aree you ready kidsssss"... Yeah we see it.

[@TheTubejunky]

@@DeathFromAbove_5.56 You do realize sheomery is technically out dated due to the drop in trust as well as pros still using that forum? Was a good run but it's been used to arrest ppl in the past so nah bro. Stop glowing.

[@scousertommy2268]

Great video Paul, I've seen this and the mycology lab 101, and it has been some of the best long-form content on this website as of late. The quality of information and production is greater than quite a few of my university professors (albeit embarrassing on their behalf). Thank you!

[@Yosef9438]

you gave just the right amount of parenthesis and unusual words, such as albeit, to prove to us you went to college, but where is your semicolon?

[@jeisuna]

Sweet! Just started in mycology and I love learning all these different agar recipes! I can’t wait to watch it!

[@DeathFromAbove_5.56]

We have to be careful relying on 20 year old growing advice when we've advanced the hobby beyond that point. I mean look it up, peroxide is toxic to mycellium....homeboy is a rookie. Wallax2 doesn't want him here. GO BACK TO PORTLAND @FUNGIA

[@ambrosioplaza3899]

This is the best free mushroom cultivation educational channel on the internet! Thank you so much Paul from Caracas Venezuela!

[@ihaveanalienhidinginmybackyard]

This was exactly what i needed this morning, you are a legend!

[@TheMycophiliac]

Amazing video on DIY Agar plates!! Thank you for sharing ❤

[@luke3892]

Love the content. It's very informative and quite enjoyable to watch. I've already learnt a bunch.

[@joshoooway]

Yo this content is beyond impressive! Informative. Concise. Creative and captivating delivery. Subbed!

[@e30kolbenkiller]

I like you Channel very much, as im currently starting to grow my own shroomies. I like the way you explain things. You are a very likable guy.

[@MikeNaples]

Awesome agar tutorial. A mountain of time and love must have gone into the creation of this much appreciated video.

[@22Webstar]

This video was a little grainy 😁 Thank you so much, loving your free education ❤🍄

[@cowlelujah5752]

Love your channel. Reinspiring me as a grower.

[@donovanholm]

Not only great as an informative guide, but an entertaining content creator. Great video!

[@gabrielfreiberg7562]

Hey dude your channel is pretty great, thank you

[@jameswheatley9883]

Thank you for your awesome videos, and your great products! Keep doing exactly what you do sir! Thank you😁 I just noticed all the sound equipment behind you 😯🔥

[@Judged-Dread]

Media flask with pouring ring is king 💪

[@lawrencered7850]

Can't wait for that library video

[@baldknobfarmstead5167]

We live up north of you in Okanogan county! I just started my mushroom growing journey and you have such great videos!

[@Ynot123.]

Great video as always! Thank you for giving us the directions and the confidence to explore while being fun and interesting. As someone who is new to this I can't understand why you don't have followers in the millions? Thanks again!!

[@RomeoTena]

Excellent, very timely, and informative video (for starters like me) giving glimpses of the shroomie enthusiasts and astonishingly, the fungi world pov. I am thinking you must be sharing a pack or two with mycophilia and maybe munchauzen. You guys are admirable in your honest to goodness presentations of your efforts in advancing the field of mushroom growing science. Congratulations!

[@zach3379]

You really do a good job of breaking it down. Appreciate the videos

[@TerpyTen]

I love your organization. I wish I had the space to do agar and cultures. I'm limited to ordering spores or cultures for now.

[@barrytrujillo]

I wish I could like this video 1000x ⭐️⭐️⭐️⭐️

[@Primitivemycology]

Love the blaagar, i have a few odd recipes i refer to as slam tech. Lol pretty much 2-3% of watever i have laying around. Have even used the water from boiling black beans, also love using the run off when i ferment wood chips for my outdoor beds. For real anything that is carb/sugar/starch/yeast based will work

[@sholtz2608]

Just received my order and I've never seen mycelium so thick and healthy in a syringe 😮🎉 100% killing it

[@tonydouglas2572]

Thanks Professor. I really enjoy your videos and all your great info. You’re a true pleasure to watch. Looking forward to more great videos.

[@TheTubejunky]

At 20:00 your adding lots of nutrients. This may have an adverse effect on your growth due to the ability of bad bacteria or fungus to thrive eating those same nutrients. Sometimes less is more. It's usually a good precedent to test this out in smaller batches and get your choice of culture stable prior to only using this recipe. Always have a backup plate ready in case things go wrong. Good luck everyone and learn before you burn. Mycology is awesome 👍😎

[@Rigooooow]

Saludos desde Chile. Me encantan tus videos! ❤🍄

[@ADDAxyz710]

Damn. Do yall be having dj sessions where you give your presentation? Nice speakers lol

[@Xulzen]

Awesome stuff! I look forward to more content.

[@StormCrow1144]

I learned alot! Thankyou.

[@ianwynne5483]

Thanks for the video it was very informative, I especially appreciated the detail on condensation, I noticed the scalpel blade was burned at the tip but no carbon deposits, I use a candle to sterilise but always have a build up of carbon. It looks really messy, especially with spores. Any tips for getting the blade sterile yet ‘clean’, thanks

[@assafLavi]

Great videos and great explanations; I’m getting eager to start playing. Can you tell some about the “yeast” part? What type of yeast are you recommending? Wine yeast is good? Or beer? Thanks allot for the great content.

[@jefflane2241]

Great video!

[@protodank]

I love that you ate the agar😅

[@yaroslav757]

You are amazing! Thank you

[@jasonrobb4948]

Dude! Hiw loud do you listen to your music? 😂 the size of those speakers! Love you content 👍🏻

[@koukouzee2923]

I made a mesh table for my SAB And I made it a little slanted I have been pooring plates slanted for months and I love it

[@user-kn9pe5ue6r]

Wow those are big speakers! Fungi Rock!

[@BadddDoggg-id4po]

Fascinating. When I first got in to growing mushrooms I didn't realize that I'd have to become a micro guy! I just started, I injected spores into my substrate x4 and nothing happened. Great first failure LOL.

[@adamlightfoot5897]

Bought from true blue 21$ for LC plus shipping. Awesome deal. My other vendor 40$ with shipping. I will come back with reviews

[@freakface1234]

So, I never have an issue with the media bottle. I always had issues with pouring from a jar. Now, i have to sut my media bottle at a tilt in my PC. I use a small jar to keep it lifted. While also keeping the lid slightly loose with foil on. Never had it boil over.

[@brescalofrio1]

thanks Paul. now i can eat mushrooms till the end

[@rewty]

Hi i have the 8qt Instant Pot Pro & wondering how long on HIGH to sterilize Agar & brf jars?

[@baldknobfarmstead5167]

I just ordered your Black Agar and Pink Oyster LC syringe this morning. I'm hoping the Black Agar works for a variety of mushrooms Lions Mane, Turkey Tail, COW, Oysters etc.

[@ntsosure3387]

Nicely done sir!

[@trepalo7]

Amazing 👏🏽

[@nimfbdhdkdm]

upload more please

[@benjaminurzua8100]

Thank you!

[@MrMalorl]

Appreciate the information! ❤

[@gszikra]

imho and maybe and all due respect and thank you for the video The video shows the petri dishes held upside down. The larger dish should be lower and the agar should coat the smaller dish the way that the ceiling of the smaller dish is coated. This way microbes cannot fall on the surface of the agar to avoid contamination. This is routinely done in microbiology laboratories. Petri dishes with microbiological medias are routinely stored like this before inoculation during storage of sterile condition, and after inoculation during incubation. Mycological specimen are usually incubated on room temperature (18 -20 dec C) bacterial media is incubated at human body temperature (38-40 dec C). At 38 deg C gelatin melts, agar-agar stays solid. Gelatin media is probably useful for mycology specimen, because it stays solid at 20 deg C incubation. Obtaining gelatin, jello, is easier in an everyday setting, and it is useful for incubation on petri dishes. It may be a kind of biiohack.

[@metermanx]

The speakers/amps❤

[@SilverPhoenix-PlazmaMoon]

Ty, this is great. I might have missed it, but... what if I have agar leftover? Can I store it for later and reheat it and pour more petris?

[@TheTubejunky]

Yes. You can but keep in mind each exposure to open air will possibly introduce contamination. It also may be a bit clumpy depending on your recipe and age of agar.

[@SilverPhoenix-PlazmaMoon]

@@TheTubejunky Thanks 🤙

[@anonharingenamn]

I use grafting tape instead of the specialized tape. I guess it's only an issue if you do no-pour agar?

[@dthomas021]

As a retired lab-rat turned mycologist, I've always used parafilm until recently. I switched to grafting tape and find it is much cheaper (0.4 cents per plate vs. 10 cents per plate for parafilm) and much faster since I don't have to cut the grafting tape into strips like I did for parafilm. Takes a little getting used to if you're already familiar with parafilm but well worth learning. Since grafting tape is so cheap I do seal every plate after the agar is set before storing. Something I didn't do with parafilm.

[@anonharingenamn]

Thank you! Yeah I have so very much to learn.@@dthomas021

[@irate74]

Nice, looking hella tweaked out in that cover photo

[@michaelmccullum7952]

do the speakers help with mushroom cultivation?

[@FRESNEL_COOKING_SOLAR_OVENS24]

Pyrex may be confusing. Not every pyrex materials is heat resistant. Has to be borosilicate container.

[@aidennymes6335]

isn't 1ml of 3% h2o2 in 100ml going to be 0,03% instead of 0,3% ?

[@_fungaia]

Oh man, yes, you're correct. That's an embarrassing and misleading mistake. 0.01%-0.05% is the correct dilution, 0.3% is way too much! I can't believe I didn't catch that. I'll post a correction in the description, thank you.

[@dummkompf]

do you have any tips for eliminating condensation when you only have a SAB to work with? pouring at 115 helped a lot but I still get some moisture and then I end up seeing contam where that moisture is sitting.

[@BadddDoggg-id4po]

Here's a SUPER THANKS! You are the best!

[@AD-gi9zg]

Like the videos. Subscribed. 👍

[@adriananaya5142]

What type of alcohol is used In the little lamp?

[@chanceshaw812]

Denatured I think?

[@nothanks9503]

One day I shall use this information to make antibiotics once society has broken down

[@gszikra]

Thank you for the video. I think, regular jello should work too. Regular gelatin melts at 38 deg C in an incubator. That temperature is used for bacterial growth copying human body temperature. Fungal cultures are incubated, usually, at room temperature 18 deg C to 20 dec C. Gelatin stays jello.

[@IAmHumanJake]

Please keep making videos

[@daniellapain1576]

I watch these videos but remember when I watched someone just take mushrooms and chuck them into a blender and layer them with some media in a pattern and then watered them to end up with mushrooms in a few weeks. How is the lab method different or superior? Just wondering if it does anything special.

[@DenverCatch22]

@_fungaia How do you prevent it from the agar from cracking once it is poured and set to dry? I noticed some plated when I go to bag them, some of the agar inside the petri dishes are cracked so to speak.

[@Mochu_s_Junkyard]

wait, no parafilm?

[@mahdishariaty9881]

He is the king👑🔥🌹

[@hajotge12]

Are you using chlorinated tap water for your agar?

[@bebophippie1781]

anyone have any insight on putting mycelium from a pf cake on an agar plate. It should work to clone right? I'm going to try no matter what just wondering what some of you think.

[@magdalenaholl5179]

You have some very beautiful mycelium agar plates my agar plates always get thick fluffy growth with long thin strands stacked on top of each other yours has a nice thick ropey growth that's very beautiful does this mean the cultures are strong and healthy or does it mean they are hungry because I've followed some of your recipes and its given me very happy looking fast growing thick and fluffy but not as beautiful as your plates how do you achieve that and is that a good thing ? I never knew that about the hydrogen peroxide I am going to give that a try I still get contamination from time to time maybe 1 in every 10 plates is still becoming contaminated with trichoderma. Could I spray say 0.5 -1% around an attempted clone that became very contaminated and grew some very scary looking stuff but also grew a spot of mycelium if I cover it spray with 1% around it then in 24hours spray 0.3% directly on the mycelium before doing a transfer to clean agar with a very low sugar content and 0.3% H2O2 will it grow without contamination spreading with it and if the contamination is all ready embedded will it atleast slow the growth of contamination and allow clean mycelium to grow ? People say just do water agar transfers but if mycelium is Gona grow on it so will other contaminants and they can in alot of cases out grow the mycelium so would you personally consider this the go to method over water agar and would you recommend anything else to help slow contamination but aid in mycelium grow eg pine contains a natural disinfectant garlic has antibacterial properties cinnamon is a mild anti fungal and ginger is natural disinfectant with 1000s more do you know of anything like this ? Does sprinkling a bit of saw dust and small grains on the agar plates before sending to colonize grain help at all? Is there anything other way to dry up the agar plates from the condensation that's not sitting Infront of a flow hood I've not got 1 and use a still air box for transfers so what's a good way to remove the condensation from my petri dishes before I transfer anything to them ?

[@gszikra]

imho and maybe and all due respect and thank you for the video Isolation of microbes: a mixed culture can be inoculated on the surface of the media about 1/3, 1/4 of . After that the inoculation loop is sterilized and from the inoculated surface using one streak the microbes can be carried to other, sterile side of the media surface in the petri dish. This produces a dilutional effect and the microbial growth shows up on the diluted surfaces a single colonies making it possible to transfer colonies of a single organism to another petri dish. Mixture of microbes can be isolated into colonies grown of single, one kind of microbes. Pure colonies can be obtained this way. imho and maybe and all due respect and thank you for the video

[@CheesieGamer]

What's the name of the intro song? ❤

[@RobertDelPrete-jc2gf]

What would you recommend for Lion's Mane agar? Thank you

[@_fungaia]

Any of the recipes mentioned work great for Lion's Mane!

[@RobertDelPrete-jc2gf]

@@_fungaia thank you, oh wise blagar pirate 🙇🏻

[@metty7528]

adding 1mL of 3% peroxide into 100mL of agar adds up to a 0.03% solution dose it not?🤔

[@_fungaia]

Yes, that is correct! You want 0.03% or less. My bad! I didn't catch the mistake until after uploading, and now it's too late to fix

[@metty7528]

@@_fungaia oh thanks for the clarification👍

[@philipagne1996]

Hey who ordered the poo poo platta

[@RichardBronosky]

33:45 Why are you discarding agar EVER? If you are willing to put Dung and Dog Food in your agar, isn't fair to say "anything goes"? I've started saving ALL of my waste agar, ALL of my contamination, and recycling it. I add agar or water to adjust the consistency as needed. AFTER cooking it at 20PSI I compare the taste to pure 2% LME water and pure 2% Honey water to estimate how much sugar it has and if any needs to be added. I'm getting LESS contamination with my "Waste Blagar" than I did when I was trying to be perfect. I'm getting so little contamination that I have nothing to add to each cycle of the agar. I have started breeding contamination in jars just so I have enough to add to my Blagar.

[@1HeartCell]

How to make agar? Well, you just link those sugars in a very specific way, et voila

[@AD-gi9zg]

You're joking. Your comment is intentionally misleading. (The gelling agent in the agar is a polysaccharide, or two polysaccharides, formed from a sugar, (galactose?) linked in long chains, I think? You have some knowledge of biology.) (Agar may be found in oriental food stores, or certain health food shops. It is only part of the culture medium mixture.)

[@KB-qk5ib]

appreciate every piece of your edu work, and the true blue ad made me laugh out loud fr. uphill both ways, with disgusting culture at both ends? too close to home matey for anyone who hasn't tried true blue's genetics yet - grab the discount and do it. not a shill but they're zero bullshit, with super fast shipping, sweet customer service if there's ever an issue, and zero misses on quality yet. I think I'm four or five orders in, tried well over 10 strains, and every one of them has outperformed competitor options, whether I liked it or not.

[@davidgates1887]

I know you try to make this funny or fun or whatever maybe you’ve been going to enough geek shows as a child but your show gives me a headache. I do learn a lot thank you a lot but geez.

[@user-df7yy4ko8p]

Actually don't like your videos. You talk to much.Should be Simple and easy about growing mushrooms on KZbin. NOT that you're a teacher at a school or college. You make your videos too complicated. 👎🤔

[@xxxhilariousxxx]

Thank you Cpt. Blagarrr! You Rock!

[@user-yn5pz3qj8r]

What is best?

[@PantsYourPoop]

Maybe I DO want to stay in a dark room and eat mountain dew my entire life! Don't judge me!😅

[@cuznclive2236]

Thank you!