HOW TO MAKE LIQUID CULTURE FOR MUSHROOM GROWING, Non-Vented Lid Jars from Start to Finish

A comprehensive overview of how I make my liquid culture from start to finish. For me, this is the first step in growing your own mushrooms at home. In this video, I focus on using NON-VENTED LID JARS. With non-vented lids, you need to equalize the pressure in your jars manually after sterilizing them in the PC. I demonstrate how to do this step-by-step and discuss the situations in which non-vented lids are preferable over vented lids. Your mycelium will not grow as vigorously with non-vented jars, so you can use a smaller gauge needle in your syringes. I typically use an 18 gauge needle. Smaller gauge needles can save some wear and tear on your injection ports.
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[@RenegadeMushrooms]

I think it's beneficial to watch my Liquid Culture 101 video first before watching this. That will help you decide rather a vented or non-vented lid set-up is best for your application. If you're a beginner, you may find the vented set-up more user friendly as there is no need for manual pressure equalization. Here's the link: kzbin.info/www/bejne/hKeqiIOVn6uNo9E

[@ChristophHalverson-iw8gk]

I think my ultimate favorite part of video two is your extremely effective but simplified way of depressurization/drawing fresh air- I’ve seen other videos which suggest silly techniques like trying to draw air out of the middle of a flame and then push it back in, this simple alcohol air filter technique seems much more reliable and simple!

[@ChristophHalverson-iw8gk]

One more question on LC types- what is your take on recipes which suggest to add a tiny amount of brewers yeast? It seems counterintuitive to add another, much faster growing, organism to the culture- even if you are sterilizing. I understand that yeast is nutritious but the amount is so tiny, I can’t fathom how it would be beneficial enough to be worth it?

[@RenegadeMushrooms]

@@ChristophHalverson-iw8gk There are tons of recipes and nutrient additives. Brewers yeast has been used for a long time, I wouldn't worry about it hurting your LC, but I like to keep it simple. 4% karo and a pinch of soy peptone, everything loves it.

[@benchadwick1754]

Great videos! Hey quick tip on the rtv silicone if you spray a teeny bit of soapy water on its surface and your fingertip (gloved if you wish) then you can shape the silicone without it getting stuck to you! lets you make it thicker without having to go wider, if that makes sense. Go on, give it a whirl!

[@malourdessun]

Thank you very much for sharing your expertise. Much appreciated ❤

[@samgodfrey8141]

Thanks for the fast replies, your 20 yrs experience is a great asset . I appreciate it.

[@vk081064]

This is by far the best video on liquid culture. Thanks for your efforts to educate beginners like me

[@RenegadeMushrooms]

Appreciate you watching and commenting. Let me know if you have any questions.

[@HeyRaeSunnyDay]

New sub here, and one word: WOWEE! This was so interesting and made so much common sense. The close proximity of your torch with the ISO had me on the edge of my seat, but the meticulous steps you took clearly map out the steps. It was particularly satisfying to watch the LC bubble when the air FAE is injected.

[@RenegadeMushrooms]

Glad you enjoyed the video 👍 I'm always here if you have questions. Thanks for watching and subbing.

[@Unclemushlovin]

Cheers new to growing mushrooms looking for good info that you have provided thanks mush lovin

[@smartjared7203]

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[@smartjared7203]

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[@davidjackson552]

Nice video. Thank you for sharing your long run of experience. That is so valuable! I have been enlightened about the lack of need for venting the lid during colonization. I've wondered about that. Two questions - Why vent the LC immediately. It could certainly be stored with vacuum. In theory, would that be best for sterility. Should you accidentally introduce a contaminant during venting, it would be better to do at the time of inoculation rather than give the contaminant a head start. Second, if you consider the effectiveness of a lami flow filter such as yours and the couple hundred or so ml of air needed to vent the jar, then the chance of a contaminant with by venting with a new or flamed needle should be miniscule. No harm done of course with the IPA syringe tech. BTW, for those without a flow hood, consider a sterile 0.2 micron Luer lock syringe filter atop your needle as you puncture the injection port to equalize pressure. They are super effective as a micro air filter. Cheers and thanks again.

[@RenegadeMushrooms]

Yes, the syringe filter would work well for venting also. Theoretically, an open needle in front of a flowhood would work as well, but I've always preferred multiple levels of security mostly because I'm working in a regular old basement with an old forced air furnace so I have some sketchy air currents. If I was in a clean room with filtered air, I wouldn't sweat it as much. As far as venting a jar right away or leaving it in vacuum, you could leave it in vacuum until you're ready to use it. But, if mold or bacteria sneaks in there during venting at any point, your jar is toast. The advantage to venting them immediately and then storing them for later use is that any contam will germinate and you'll know not to use that jar. I've vented them immediately, and stored them for months before inoculation with no issues using this method.

[@mattnsim]

Never new about master jars! Thanks mate

[@glennplatvoet7111]

Made flo hood work's well glad i watching again soon I'm going to do long term jars now we're going thanks for videos really helpful grow on .

[@RenegadeMushrooms]

Awesome, having a flowhood is a game changer.

[@loricox3833]

We are just starting out and following your videos as gospel! Thank you so much. Just not sure at what temp range we should store them until ready.

[@francisr6804]

Awesome video! (beginner here) I thought of a solution that might work: what if you take a syringe needle, you put a micropore tape on the needle large opening (the needle-syringe connection side), you pressure cook the jar with the needle in the injection port (to allow gas exchange) and when the cooking is done you remove the needle. Do you think it could work?

[@RenegadeMushrooms]

Yeah, it might. I actually really like that idea although I would probably go 2 or 3 layers of micropore to be safe. Or maybe glue a small filter disc to it. I'm gonna try it 👍

[@francisr6804]

@@RenegadeMushrooms Awesome, let me know if it works!

[@bestheirs8999]

@@RenegadeMushrooms Can one use plastic jars instead of glass jar?

[@RenegadeMushrooms]

@@bestheirs8999 Plastic jars are ok as long as you use thick PP5 plastic, but glass is superior in my opinion.

[@Junzig]

Great video. Thank you

[@glennplatvoet7111]

That's slick thank you

[@chillville5571]

So informative, thanks for dropping the knowledge. once i slow down and learn its so easy. So micropose, i love them! Question, can you put an injection port on both sides or is that overkill?

[@RenegadeMushrooms]

Two injection ports isn't really necessary. I always look at it like any additional holes in your lids are potential contamination points.

[@angel2jimenez219]

@@RenegadeMushrooms i chill ville means like 2 on the same hole 1 on top and one on the inside, the same way the rtv is done but with the self adhesive ports

[@RenegadeMushrooms]

@@angel2jimenez219 Not necessary.

[@davidblack5551]

The. MASTER 👍

[@dudetapedtoafridge3073]

13:10 i like to put a thin folded strip of aluminum foil under the lid seal before the pc and simply pull it out after it cools and I've never had an infection problem. Especially when you put a pressed cotton ball over the vent of the pc for extra clean air pullback haha

[@dudetapedtoafridge3073]

That way you just gotta pull the tab without even lifting the foil covers if it's sticking out enough and then simply tighten and boom done. Saves me lots of time with large batches!

[@RenegadeMushrooms]

Good tip, thank you 👍

[@J155P]

More questions for you...why couldn't you just sterilize the needle without the plunger attached? Is it because you wouldn't be sterilizing the needle all the way up to the plastic fitting? Also, since you're still introducing the air that's in front of your hood, wouldn't it be nearly the same risk as if you just cracked the lid briefly or no? Also, how would you do this with a still air box given the use of alcohol and flame? Thanks again.

[@RenegadeMushrooms]

Yes you run the alcohol through first to sanitize the entire pathway of the incoming air, needle hub, syringe, etc. It is not the same as just cracking the lid in front of the flowhood because you get an extra level of protection with the syringe because all of the air flows through the alcohol sanitized pathway of the syringe, polyfil, needle etc. Just a more controlled way of doing it. Everyone loves to think of ways around doing this, but it's always worked for me so I've stuck with it. I really don't find it difficult, but it's not something I would attempt in a SAB. If you are using a SAB, just use a filter disc in your lid in addition to the injection port and problem solved.

[@TheDMTLover]

Good tricks. Thx. 🙂

[@malekith8215]

Thanks for the cool video. Never heard before that lc doesn’t need air exchange. One question. Couldn’t you just use vented lids and keep them covered with aluminum foil really snugly after you take them out of the pc? You only had to remove the foil when you inoculate or draw from the lc. I guess that wouldn’t completely stop the air flow but limit it enough to get some of the non vented lid benefits. But after all you put aluminum foil over the jar lid anyways once they go into the fridge for storage.

[@RenegadeMushrooms]

The main drawback to having vents in the lids is the vents are prone to have mold germinate on them if you don't keep them dry. So, if you're not extremely careful when pulling syringes, you can continually soak them with LC and covering with foil can actually hurt in that case because it will cause the filter to remain moist. Once mold germinates on your filter it will punch right through to your jar and its game over, so you really have to focus on keeping those filters dry if you're going to use them. For this reason, I only use filters on jars where I'm going to dump the whole jar to substrate like with my mycelium flood method for Cordyceps and Enoki.

[@malekith8215]

@@RenegadeMushrooms Hello Thx you very much for the quick reply. Yes. Actually I just started growing shrooms. I used 2 layers of micropore tape for venting , but those are constantly wet. I guess because the liquid is breathing through them. Non vented lids are probably a great alternative. I also used spore solution to inoculate the liquid culture (as recommended in a certain book). Problem: The spores multiply but don’t germinate. Lc works much better to inoculate lc. 😉

[@jbb3141]

Have you tried Rutand RTV 500° F Clear Silicone for the self-healing injection ports? The benefit is that the drilled hole below can be seen otherwise it has the same max temp as high temp red gasket RTV silicone.

[@RenegadeMushrooms]

No, I never have, but I'm sure it would work. I've never really had a problem not hitting the hole though with the red.

[@glennplatvoet7111]

What is the best way to make a flow hood can i get a plain somewhere thanks for your videos all good know how I'm just getting going with it

[@RenegadeMushrooms]

There are lots of designs out there. I prefer a centrifugal fan setup myself. I explain how I built my hood in this video: kzbin.info/www/bejne/d3acpYNsZq-ahpo

[@lizzp9830]

Thanks for sharing your experience! I have a couple questions and forgive my ignorance it’s been a long time since I’ve had any science classes- I don’t understand how the air stays sterile after the plunger comes out. Why not use the plunger to push the enclosed sterile air into the jar? Would it be possible to seal the vent hole after sterilization without contaminating the jar?

[@RenegadeMushrooms]

There are a lot of ways to accomplish the pressure equalization step, this is just what has worked for me for 20 years so I feel comfortable that it will work for others consistently. The air in the syringe stays sterile because you are flaming the needle as you pull back the plunger. It takes a lot of air to equalize the pressure, so if you left the plunger in it would take 4 or 5 syringes worth to equalize one jar. This process has redundant steps to ensure clean air enters the jar.

[@jaydenallanach969]

Do you think the pickle jar lid and a hole with medical tape would work?

[@RenegadeMushrooms]

Probably as long as you incubate in a storage tote. I would go with two layers of medical tape though. Also I wouldn't use a jar bigger than a quart with just a single hole. Anything one quart or less should be fine for air exchange.

[@kumhoshin8736]

I have learned a lot from your presentation. I always thank you for your presentation. I have a question, after making cordyceps liquid culture, how long can I keep and use it?

[@RenegadeMushrooms]

You want to use it within 2 weeks for best results. If you need to store it longer, it's best to keep it in the refrigerator. A fresh high quality culture will store much better than an older weaker one, but, in general, cordyceps cultures senesce much more rapidly then other species. So, the longer you store them, the weaker they get.

[@mse1333]

Hey Renegade…..can you use spores to colonize your liquid culture? Love your vids!

[@RenegadeMushrooms]

You can as long as the spore syringe is sterile, it works great. Thanks for watchin 👍

[@mse1333]

@@RenegadeMushrooms hey Renegade, I subscribe to over a hundred channels covering various subjects. You are the only one that I know will answer a question. Great job! Really appreciate it.

[@AM2PMReviews]

How do you pressure sterilize with an instant pot?

[@RenegadeMushrooms]

I've never used an Instant Pot so I don't know. I'm sure you can find some vids on YT.

[@AM2PMReviews]

oh yeah I see a lot of videos. I didn't know it was even used but I guess some people prefer it. I am going to try to grow lions mane for my first mushroom. any tips? @@RenegadeMushrooms

[@RenegadeMushrooms]

@@AM2PMReviews Lions Mane vid: kzbin.info/www/bejne/hF60oZVpgJ14gpI I think some people prefer the instant pot because it's cheaper than a real pressure cooker. If you're going to get somewhat serious about this hobby however, spending a couple hundred bucks on a 23 quart Presto pressure canner is well worth it imo.

[@samgodfrey8141]

I torched a old needle and just for fun poked it in the cover of a plastic petri dish. To my surprise it went right through like butter , leaving a perfect hole the size of the needle. This got me to thinking why couldn't I draw a syringe of new LC and violently inject into the petri dish to break up the mycelium and then suck it back into the syringe and inject it back into the jar of LC to inoculate it. Pick this idea apart and let me know if you think it would work.

[@RenegadeMushrooms]

Sounds solid to me, you should try it. I would melt the hole near the edge of the plate and make sure it's sealed really well with parafilm. That will allow you to tip the plate on edge and make it easier to pool the LC and suck it back out. Let me know how it works 👍

[@philq01]

I noticed you do not use magnetic stirers. Is that not necessary

[@RenegadeMushrooms]

They are great, but not necessary. I just shake/swirl my jars by hand and it's always turned out fine. I would say go for it though if you have the money.

[@ymm424]

what size are those black self-healing plugs please? I just searched for them and found they come in two sizes.

[@RenegadeMushrooms]

microppose.com/products/adherable-injection-ports-for-plant-mushroom-tissue-culture Don't forget you can use promo code "RENEGADE" at checkout to save yourself 10%.

[@mtrobenazz]

When you reuse your polyfilled syringe. Do you just refill it with iso and flame it, or do you need to run it through the pc as a precautionary measure?

[@RenegadeMushrooms]

Just refill it with iso, push through to clear all the iso, then withdraw plunger while flaming. I've never run it through the PC. Works every time for me.

[@crypto.entrepreneurs]

Thanks for sharing your knowledge. I was wondering if I can replace polyfil with cotton?

[@RenegadeMushrooms]

Cotton, being a natural fiber, absorbs water and is more prone to contamination. Polyfil is cheap and definitely the way to go.

[@crypto.entrepreneurs]

​@@RenegadeMushrooms Thanks for the quick response. I was asking this because polyfil is hard to find near me, only online. I also understand that reuse is the way to go. However, as I don't have polyfil, I'm going to do a test with cotton, but I'm not going to reuse it. Thank you, I learn a lot from you

[@RenegadeMushrooms]

@@crypto.entrepreneurs You can also use 2 layers of micropore or cloth medical tape if you can find that.

[@crypto.entrepreneurs]

@@RenegadeMushrooms Yes that I have. I'll try it that way too. Thanks

[@calichojack991]

Do you need a torch or could you do this with a lighter?

[@RenegadeMushrooms]

You can do it with a lighter, but a small torch works much better. You don't need a giant propane torch like I have. A small butane torch is fine.

[@J155P]

Is the purpose of doing this just so you don't have to keep buying liquid culture? If I were just getting started and wanted to grow one kind of mushroom, would I just buy some liquid culture and keep repeating this process whenever more is needed? Thanks

[@RenegadeMushrooms]

Yep you got it. With this method you can expand one LC syringe into pints, quarts, even gallons of liquid culture to inoculate your spawn with. Much cheaper than continually buying syringes, at least for species you think you are going to grow a lot of.

[@superagentHH]

Yeah, love your videos! Question: LC and stuff all works perfect and now i wanna store some like you are describing. Can I not just use a vented lid and cover it with scotch tape whenever, and when drawing just remove the scotch tape? I know there's a lot of things that work - but would love to know what you think or thought about it. Thank you 🙏

[@RenegadeMushrooms]

You could try it, but my concern would be that the filter patch would grow mold under the scotch tape. The filter patch needs to exchange air and do it's thing to keep it dry. If you cover it with tape, the excess moisture build-up from lack of airflow may cause mold to germinate on it contaminating your jar.

[@FelixNothus]

Question: Couldn't you just leave the lids slightly ajar in the pressure cooker with the foil over the top of them and then just seal them in front of a flow hood or clean room?

[@RenegadeMushrooms]

Technically yes, as long as you seal them in front of a flowhood. The problem is that your pressure cooker pulls in outside air as it cools, so you are risking contams entering your jars in the PC if your lids aren't sealed, although the foil would help prevent that. I work in a dusty old basement, so even with a flowhood I prefer to leave my lids sealed. This method has always worked flawlessly for me and others, but there are many ways to accomplish the same goals in mushroom growing so I always encourage everyone to do what works best for them.

[@FelixNothus]

Thank you for your answer@@RenegadeMushrooms I'll have to experiment then and see if there's a more reliable than not way of this method.

[@DukeTurnip]

Thanks again for the video. I want to use the equalizing method you demonstrate in this video but don't have a laminar flow hood. If I do it inside a SAB, could I then wait to see if anything else starts growing in the jars before innoculating them or would you recommend innoculating right away. If OK to wait, any idea how long I should wait before expecting to see contaminates growing?

[@RenegadeMushrooms]

That method will work fine in a SAB, just be careful with the flame and alcohol vapor. Flame outside the SAB, then go in and equalize. I would inoculate right away if it were me. Just always test a bit on a small grain jar before going big time.

[@danherring5676]

So, I've now made two nutrient jars for LC, pressure cooked them at 15psi for a half hour, and both times, about 2/3 of the liquid evaporated from the jar. Today, that happened, and when I removed the jar, the bottom cracked off! It was a real one pint Ball canning jar, too... I let it cool off in the cooker, but it cracked after I lifted it out. Don't understand what's going on.

[@RenegadeMushrooms]

I'm not sure either. What lids are you using? How full are your LC jars with liquid, and is there water left in the bottom of your PC when the cook cycle finishes? Also, what are you using for a heat source? I've run thousands of jars, and never had one break.

[@AlexWondergem]

thankQ 4this!

[@rickyonoroto2094]

Can spore syringes be used with method?

[@RenegadeMushrooms]

Yes, as long as the syringe is sterile.

[@lycanzhp1141]

Great video, thank you. Question please. You are allowing clean air ( a few CC's ?) into a LC jar that is under vacuum to equalize the pressure in the jar to match the outside atmosphere. Then you inject about 10CC of LC back into the jar causing a positive pressure situation in the jar. Would it not make sense to just skip the equalization step because you are going to add 10 CC of fluid into the jar, there by equalizing the pressure that way. ?? Thanks

[@RenegadeMushrooms]

It's more like 200+ ccs to equalize.

[@lycanzhp1141]

@@RenegadeMushrooms Wow, thats a lot of vaccum ! I wasnt aware it was so much. I understand more clearly now. Thank you for the quick reply. I enjoy your videos so much, I re-watch them every time I go to perform some mycology steps. I'm still a noob. Oh, I tried your trick about injecting sterile water into a colonized grain bag and then aspirating the water back out and then injecting it into a fresh grain bag, It worked!!, no contamination at all. Thank you again :)

[@RenegadeMushrooms]

@@lycanzhp1141 Awesome, glad it worked for you 👍

[@archieh4058]

Are the RTV lids dishwasher safe?

[@RenegadeMushrooms]

Yes I would think so, but I always hand wash them, only take a second.

[@johnhall9378]

Could you just use a vented lid, then after the jars cooled and pressure equilibrated, seal the filter disk with tape or RTV ?

[@RenegadeMushrooms]

If you were going to do that, then you would just use a lid with a filter disc in it like I show in my 101 video: kzbin.info/www/bejne/hKeqiIOVn6uNo9E

[@cliffmello]

Hey boss, were can I get empty syringes? You order online? Thank man. Keep up the great vids

[@RenegadeMushrooms]

Thank you👍 I ordered a 30 year supply 15 years ago from a medical supplier in Jersey, but they seem more difficult to find nowadays. Mycology suppliers seem to be the best bet now. Check out Shroom Supply or other online mycology stores.

[@stayanddrown]

Hey, I had a quick question. Would having a sterile syringe & needle right out of the package eliminate the need for the polyfil and flame? I would imagine so, just curious if you've had a bad experience with it. Also, great video! Thanks for the helpful information!

[@RenegadeMushrooms]

I've never trusted just sucking in air from in front of my flowhood right in to my LC jars. The alcohol soaked polyfil serves as an insurance policy to catch any contams that may be present. It's never failed me in 20 years, so I stick with it. A needle right out of the package would not need to be flamed. Thanks for watching 👍

[@stephenwalther9702]

​@@RenegadeMushroomsaway another question, please: why couldn't you tape over a vented lid filter with regular tape, to prevent further air exchange?

[@RenegadeMushrooms]

@@stephenwalther9702 I don't see any reason why you would want to do that. The only reason you wouldn't want a filter in the lid is because then you don't have to worry about getting it wet when you shake your jar, potentially causing mold germination. Just taping over it won't prevent that.

[@wickedjack1222]

Wouldn't just squirting (the jar actually sucks) the LC syringe equalize the pressure inside the jar ?

[@RenegadeMushrooms]

Not even close, you could dump a full 20cc syringe in and still have vacuum.

[@douglasr.c.5622]

Do you calculate the 4% sugar content based on weight ?

[@RenegadeMushrooms]

No, by volume.

[@douglasr.c.5622]

So how much sugar would you add to 500cc of water ?

[@RenegadeMushrooms]

@@douglasr.c.5622 20 ccs if you're using light karo, plus just a pinch of soy peptone if you have it.

[@douglasr.c.5622]

Great ! Thank You

[@Akasha_Silver]

Hey,how much water & Karo syrup would I use to make only 2 pint jars? Thanks

[@RenegadeMushrooms]

You want a 4% solution so just take your total distilled water volume and multiply it by .04, that will be your amount of karo. Not perfect but close enough 😁 I usually put 300 ml. of liquid in my pint jars so 300x.04=12. So add 12 ml. of karo to each jar if you go with 300 ml. You can go a hair over that without hurting anything.

[@Akasha_Silver]

@@RenegadeMushrooms thanks

[@rfyock009]

Ty so much! What micron is micropose? The reason for the poly fill syringe?

[@RenegadeMushrooms]

Sorry for the late reply, I missed your comment. The microppose filter discs are .22 micron. The polyfil syringe process I demonstrate is the technique I use to manually equalize the pressure in liquid culture jars with no filter disc in the lid after running them in the PC.

[@Jason-oy6vp]

@Renegade Mushrooms: Hello. New to growing. Am I wrong to think I could affix a 2u filter between a needle and plunger-less syringe and then use it to equalize pressure? Serious inquiry.

[@RenegadeMushrooms]

Yes, as long as you assemble everything in front of the flowhood, that should work fine. The alcohol soaked polyfil is an old school poor man's trick from back in the Shroomery days. It's always worked flawlessly for me so I stuck with it. Try the filter deal and please let me know how it works for you.

[@Jason-oy6vp]

@@RenegadeMushrooms Thank you! I will.

[@haroldjoseph3596]

Hey this question doesn’t have to do with this video but I figured I’d ask you cause you know your mushrooms!! My question is when you’re making agar cup recipes (if you do that) what could be used as a replacement for a media bottle? Those bottles are wicked expensive. Let me know if that’s something you do. Thanks man.

[@RenegadeMushrooms]

I've worked with agar, but never poured my own. Pretty much everything I do is LC. Hopefully another viewer will chime in. You could always ask the people at Shroom Supply too, they might have a suggestion.

[@professored7169]

If you have have a pc then do no pour agar, its what I do because I use a SAB

[@toddwmac]

Hey Harold.... FWIW I've been using the bottles that Kombucha is typically sold in and the grocery. They are about 500ml, clear and typically built like a tank. Their stickers/labels are a bit of a pain to remove so I steam them for a few minutes, peal the (usually clear) wrapper. To get the glue residue off I wipe with something like Goof Off (acetone) or mineral spirits/lantern fuel/kerosene Never had an issue and I figure I've saved myself enough for some great MSS'. Needless to say, you might want to do this away from your lit torch. :)

[@mse1333]

Hey RM, so why do you only fill the pint jars to 300ml, any reason? Also if you were to fill the jars almost completely, there would be no air so little to no vacuum would be created during the PC processing, so maybe no need to deal with equilibrating the pressure?

[@RenegadeMushrooms]

There would still be enough vacuum that you would need to equalize. You could put a little more liquid in there if you wanted, but overfilling them can cause alot of issues, so if you need more volume, just make more jars.

[@RobBanks81]

If the jars were filled almost completely, would there be enough oxygen for a year's storage?

[@RenegadeMushrooms]

@@RobBanks81 It's possible they may be starved for oxygen if the jar was too full. It could cause all kinds of problems actually, no reason to do it. I've always filled them to 300 ml. for a pint and 600 ml. for a quart and never had any issues.

[@paulrush9166]

I love this shit

[@Martha_mycology]

Hey 👆🏻👆🏻👆🏻👆🏻👆🏻👆🏻look up the handle for good shrooms grow kits spores

[@kevinabood3598]

liked and subscribed

[@Fall3nL3g3nd]

Do you think if i make it with Tyndalization instead of pressure cooker will it be fine?

[@RenegadeMushrooms]

That's a very interesting idea. Theoretically that should work. The only issue I could see is that it may lead to excessive caramelization of the sugars in your solution, but that may not be an issue. Try it and let me know if it works 👍

[@DukeTurnip]

Using a pint jar and your exact proportions, do you have any idea what volume of air would be needed to equalize a jar? If you were to use a large cc syringe could you not remove the plunger when equalizing? That way any air entering the jar would have flowed through the hot needle? What do you think? Also can you suggest location to purchase 12/14 gauge needles? An internet search isn't bring up much larger than 18 gauge.

[@RenegadeMushrooms]

I've tried it with up to a 50 cc syringe and it wasn't enough. Maybe 100cc would work? 14 or 16 gauge needle is fine, 12 is a little big so I need to edit that comment. Shroom Supply has 14 and 16 I believe.

[@mse1333]

Hey RM, is there any disadvantage of using the good plastic lid (with the appropriate silicone seal) when making non vented lids (using the red RTV)? Or do you recommend metal lids? Thanks!

[@RenegadeMushrooms]

@@mse1333 If you're going to go with plastic lids, I highly recommend the Masontops Tough Tops lids. I wouldn't trust any other plastic lid set-up I've tried for LC. Or just go with the old school metal canning lids.

[@daveeatmon4718]

Plus I the wild, millions of spores are produced with only a small fraction of them ever colonizing and producing fruiting bodies for us to enjoy.

[@mattemmerson5877]

Do you reuse the foil lids? I never thought about that.

[@RenegadeMushrooms]

Yes, I re-use them many times, until they get brittle.

[@frasercoasthomemaintenance7701]

Hi, Can I inject the liquid mycelium straight onto wood or straw.

[@RenegadeMushrooms]

You can, but you need to use a lot of LC if you're going to spawn it to bulk substrate. It will be slow to colonize as well, so it's not very efficient and leads to increased risk of contamination. That's why the general rule is LC to Grain to Bulk Substrate.

[@diverwa]

Obviously this is working for you but I have a question, after relieving the vacuum and inoculation the jar it's then under pressure. Should you equalize again?

[@RenegadeMushrooms]

Not necessary, all you need is that initial equalization to relieve the vacuum. Just allow it to colonize for 2 weeks or so and it's ready to use for up to a year with no additional pressure equalization needed.

[@diverwa]

@@RenegadeMushrooms Thanks for the follow-up. One more question, have you ever tried cooking your grain in agar befor inoc to speed up spawn?

[@RenegadeMushrooms]

@@diverwa No I haven't, but let me know how it works if you try it.

[@seanmblaine5953]

Why not use an autoclave?alcohol will not sterilize anything

[@RenegadeMushrooms]

What part of the video are you talking about, what am I supposed to put in an autoclave?

[@blenderbenderguy]

I have the same torch..... I keep knockin it over.... it's time for a smallish alcohol lamp.

[@RenegadeMushrooms]

Dang lol, I've never knocked mine over. But you do definitely have to go with the fatty propane bottle if you're going to rock that torch for the stability factor.

[@grizzlybeast5]

Is 15 psi due to your elevation? Or just extra sterilization steps? Because I pressure can food and I'm at 600 feet above sea level and it calls for 10 PSI for about the same amount of time. I'm actually binging your videos right now, because I have done some decent mushroom grows, but I want to get more yield for less purchase.

[@RenegadeMushrooms]

Cool, let me know if you have any other questions. My PC rockers are set for 15 psi, and that's fairly standard for most mushroom growing sterilization procedures. I'm not an experienced canner myself, but I'm sure 15 psi is overkill in some situations. I was taught to run at 15 psi, so that's all I've ever done, and I rarely get contams so I've just stuck with it.

[@grizzlybeast5]

In two and a half hours of time, I have five pages of notes! I live in upper Michigan and I thought the coolest thing (besides all your content) was resting the grains. "So I'm going to go ice fishing for 3-4 hours"

[@RenegadeMushrooms]

@@grizzlybeast5 Ahh cool, yes fishing is my other passion. I fish all year, but I love to ice fish. I have a good friend in the U.P. and he always brags to me about their long ice season up there. In Western NY, we're lucky to get 2 good months of ice, but I hit it hard when we have it.

[@grizzlybeast5]

LMFAO! He can brag all he wants, but if you have to add an extension to your power auger, ice fishing isn't as fun!

[@RenegadeMushrooms]

@@grizzlybeast5 Agreed lol

[@treehouseconstituents6402]

Questions please and if you could help it would be amazing thank you. Time is of the essence per se lol. 1. You said no direct light, however would using my flashlight to inspect everything in my lab thoroughly cause any issues or retardation in growth. 2. Can the GS, LC and Agar all be stored at the same temperature or does one prefer different temps during different stages. If so what temps per stage. 3. Using 18g needles, I'm getting pieces of my silicone in the...well everything, the needle cuts out a tiny piece. I pulled an Agar specimen out to inspect it and noticed FAE, so I looked a little closer and there's a hole clean through my S.H.I.P, I generally make them atleast ¼" on both sides of the mason lid. Any advice?

[@RenegadeMushrooms]

I'm currently using 18 gauge needles as well. All injection ports will wear out eventually, but a good dollop of high temp RTV is the most durable in my experience. I would say I get about 50 injection/extractions out of mine before scrapping. If yours are wearing out prematurely, make sure you're using high temp RTV and putting it on thick. Your flashlight comment cracked me up because I do the exact same thing and no, a brief exposure doesn't seem to hurt them. Also, I store everything at ambient basement temp which is 60 to 70 F depending on the season.

[@treehouseconstituents6402]

@@RenegadeMushrooms thank you so every stage likes the same temps. Also I'm using high temp RTV Silicone and it's been the first time I put the needle in. Same with the S.H.I.Ps on some of my plastic lids. First poke and I can visually see the hole. Also it's not that brief of a light show for my babies. I went full production from the start knowing I should start small. I've watched hundreds of hours of videos and read 3 books 2 by Stamets and the mushroom grow Bible. I felt confident enough to dump a handful of cash down on everything I needed including a LFH. I have 25 56qt monotubs. So when I go into the lab I turn the lights on and spend about an hour maybe more inspecting agar cups, LCs and GS. So I'm in there for a few I'd that too long or should I adjust my tek. And a question I forgot to ask... I notice you like to shake up your GS allot earlier than most growers, I understand all the principals involved as well, however, what is too much shaking, when is too early and when is too late. I love your channel and I'm very familiar with good ol awkward Gary. Thanks for your quick response. Also my light is an Olight Warrior 3S so it can go all the way down to half a candlepower and run 1watt. So that should be ok? I was thinking of getting an Ultra Violet flashlight and green laser for some of the inspections, what do you think of this method? Last thing brochacho, I've been manufacturing a synthetic dung via nutrient supplementation, I use it as a nutrient broth when gaining field capacity in my Bulk Sub and it works great! Any time I notice contamination on my agar plates, I drop a few drops of Colloidal silver directly on to the contam, it's worked for everything but yeast and the mycelium doesn't care much and bounces back very quickly. I had a contaminated plate I soaked in Colloidal Silver and then put it up and actually forgot about it for almost a month or more. I stuck my spore syringe into the grain spawn just cuz and it sucked the last of my solution out so fast it hurts. I thought I'd lost my genetics, but I just cut 8 clean plates from that contaminated and treated with CS plate. So I saved the genetics with colloidal silver. I highly recommend CS over H²O² or anything else for that matter. I am going to perform more experiments with it soon lol. Sorry for the novel

[@RenegadeMushrooms]

@@treehouseconstituents6402 That's a cool idea with the silver. I know it's a potent antibiotic, but never heard of anyone using it for mushroom cultivation 👍 I don't think that brief exposure to a low wattage flashlight would hurt your grows. If you held it on one spot for like 20 minutes maybe, but not just glancing around checking grows. Not sure what's going on with your injection ports, but you may just be over-analyzing. You will always be able to see where the needle penetrated, but there shouldn't be a hole that would allow contams in. If there is, your RTV isn't curing properly or something. I've tried a lot of pre-fabbed rubber injection ports and they all pretty much suck. My favorites are the self-adhesive ones from Microppose.com, but you can't beat RTV for durability.

[@treehouseconstituents6402]

@@RenegadeMushrooms thank you, and I could see light through the hole. My assumption is that most of the times my needle took a "core sample" lol the core was extricated. So. I just fully processed 32 grain spawn today I have to go to bed it's 5am lmao I didn't even realize. Can't stop mid project though fuck. I can't leave my FC grain out all night all I can do is jar it FML

[@mattemmerson5877]

Does your LC get darker after it's been pressure cooked? Mine did but I used extra light malt extract and peptone for the first time and I don't know if it's normal for it turn a dark amber color, like iced tea.

[@RenegadeMushrooms]

Yes, that's normal. Make sure you are only PCing your LC for 25 minutes once you reach full pressure though. If you go much longer it will start to caramelize your sugars and make your LC even darker. It will still work, but this is generally not recommended.

[@mattemmerson5877]

@@RenegadeMushrooms Ooops! I did over an hour. Thanks for the heads up. If it is cloudy after my LC transfer, that indicates contamination, right?

[@RenegadeMushrooms]

@@mattemmerson5877 Yes, usually bacteria

[@mattemmerson5877]

I just did a new batch of LC jars. Half were extra light malt extract and peptone. Half were light corn syrup and peptone. The malt ones came out of the pressure cooker dark and cloudy. The corn syrup ones are darker than they started but not like the malt, but the liquid is clear. So it'll be harder to detect contamination when inoculating the malt jars. I hope this makes sense.

[@RenegadeMushrooms]

@@mattemmerson5877 It does make perfect sense, I tried DLME many years ago and I wasn't really a fan for that exact reason. The mycelium seems to love having all those little nuggets to adhere to, but I didn't like the cloudiness.

[@rtbkbulliesandpitbulls1714]

By equilizing the jars and then adding liquid culture arent you creating a positive pressure ? As the liquid you're adding raises the level of liquid in the jar the air has no way to escape or equalize thus creating positive pressure. Just an observation i just deal with preassure and vacuum stuff in hvac trade. Also i know you said this works and i wont argue that but as liquid changes temperature that influences mulecular movement which in terms affect pressure. While you're equilezed at 78 degrees Fahrenheit when you put that tub in the refrigerator and cool the liquid molecular movement will slow and thus creating a vacuum so it would need to be equilized again. Vise versa once you get it out of the fridge molecules will move faster creating a positive preassure and would need to be equlized again, a good example would be their air on your car's tire when its cold the air pressure drops giving you a low pressure symptom and you add some air but as the car rolls and creats heat on the tire do to friction or the temperature changes outside you'll be over pressure as molecules move faster. Maybe a breathable filter patch is needed after all to have a fully controlled chamber.

[@RenegadeMushrooms]

Everything you said is correct, but you're overthinking it in terms of practical liquid culture production. Small pressure differences always seem to equal themselves out in these jars. My guess, although I've never tested it, is that most of the equalization happens through the micro-fissures in the injection ports when you're pulling syringes from the jars. The main thing is to equalize the initial pressure after the PC run, after that everything else seems to work out in the wash.

[@rtbkbulliesandpitbulls1714]

@RenegadeMushrooms awsome, I was just throwing some physics out there. I watch your channel alot and I learn alot from it. Thanks for addressing my comment properly.

[@absofjelly]

Can't you just crack the lid in front of the laminar?

[@RenegadeMushrooms]

Yes, theoretically that would work. But a lot of people don't have a flowhood, and even with one, any time you compromise your jar lid, you greatly increase the risk of contams. All it takes is one spore off your hand or the jar to blow in there and it's over.

[@cameron4994]

Just a quick thing i noticed, you are flame sterilising your needles, and then wiping them with alcohol which only sanitises, your better off just flame sterilising 👍🏻

[@RenegadeMushrooms]

It rapidly cools and wipes the soot off my needle, allowing me to work faster and cleaner. Also 20 years tells me it has no effect on contamination. All of the injection ports that the needle passes through repeatedly are alcohol sanitized as well so it's irrelevant really.

[@cameron4994]

@@RenegadeMushrooms if it works for you that's great 👍🏻 just clarifying there is a difference between sanitised and sterilised, although the inside of the needle would still be sterilised as it hasn't touched the wipe. Happy days a mush love. Nice video btw!

[@ricardocarrillo4613]

What are the components and proportions you use for your liquid culture?

[@RenegadeMushrooms]

Per quart jar: 600 ml. distilled water 25 ml. karo (corn) syrup .25 g. soy peptone The soy peptone is optional

[@ricardocarrillo4613]

@@RenegadeMushrooms Thank you very much and congratulations, excellent content. +1 sub.

[@wild_for_nature9932]

This has always been so crazy to me. All this STERILIZATION to grow something that naturally grows in a non sterile environment. 🤯🤯 It's really hard for me to comprehend the science behind it. It's like, I get it but, at the same time, I don't 😵‍💫🤪

[@RenegadeMushrooms]

It's the conundrum of intensive culture. You can use simple, non-sterile techniques and successfully grow mushrooms outdoors, but your yields will be small compared to your effort. In order to produce high yields, we use artificial climate controlled environments and super nutrient rich substrates. These factors, if successful, will produce tons of mushrooms. But in order to do so, you also have to take steps to control the other creatures that thrive in these conditions, namely bacteria and mold. Hence the need for sterility.

[@magdalenaholl5179]

Instead of that dodgy step to get clean air into your sealed jars just put a 0.02ug on with the needle and poke that into your injection port that will allow sterile air to get inside the jars

[@RenegadeMushrooms]

Yeah there are lots of ways to equalize pressure but this really isn't that dodgy. It's pretty simple and effective actually and being comfortable with flame and 70% isopropyl is part of the game for home cultivation.

[@RentAsunder0]

That micropose stuff costs an arm an a leg 9$ for 1 plastic petri dish yea right😂😂😂😂😂😂😂😂😂😂😂😂😂

[@RenegadeMushrooms]

I'm not sure where you're getting that from??? They sell a case of 500 plates for $60. I'm thinking your math is a bit off.

[@chazdaddy6076]

Do you think 4 cc spore syringe would be enough to make culture? I have some left from a recent batch and want to give it a try. I have like 5 more different ones coming so screw it

[@RenegadeMushrooms]

Yes, it will work as long as the spore syringe is sterile.

[@OilPaintingWorkshop]

@renegade mushrooms I like what you said about not needing to keep the liquid cultures in complete darkness. thank you for that. I was wondering is this also true for temperature? Does that also not really matter or is there a temperature range that is really ideal for keeping newly formed liquid cultures? Thanks a lot I love your channel.

[@RenegadeMushrooms]

Glad you enjoy the channel 👍 My basement ranges from 60 to 70 F depending on time of year and I see little, if any, effect on the growth of my LC in that temperature range. I would say anywhere from 50 to 80 F is fine.

[@OilPaintingWorkshop]

@@RenegadeMushrooms thank you for taking the time to answer my question

[@rickfoss1136]

Best mushroom channel on KZbin. I’ve learned 95% of my mycology knowledge from this channel. And any time I have a question you usually have a video to answer it. When your equalizing the jar with the needle syringe, guessing just putting the needle into the jar for a few seconds, the vacuum inside the jar will pull out the amount of air it needs without any need for a person to manually push the plunger?

[@RenegadeMushrooms]

That's really nice of you to say, thank you 😁 Yes, it only takes a couple seconds for the pressure to equalize, no plunger needed.

[@rickfoss1136]

@@RenegadeMushrooms thanks for the reply! Keep up the great work and thanks for sharing all your knowledge!

[@addammadd]

(Currently just a spectator in the depths of a monthslong binge on this subject, excuse my ignorance.) Would adding some amount of H2O2 suffice to “vent” (or essentially add back both oxygen and volume to avoid hypoxia and a vacuum) rather than this intense process you’ve got here? I imagine since you’ve been doing this forever you have some reason(s) why this isn’t done. Thanks for all your work man, this content is fantastic.

[@RenegadeMushrooms]

That's an interesting idea, but you would have to add 50 ccs or so of H202 just to equalize a pint jar. I'm thinking that would cause issues with growth. You would need a monster syringe, or have to do multiple injections. This air venting method might seem complicated in the video, but it's actually pretty easy once you do it a couple of times. Thanks for watching 👍

[@SirBoden]

Minor point: Heat sterilizes, alcohol sanitizes. Wiping a heat sterilized needle with alcohol downgrades it to sanitized. This is good enough in this situation.

[@jasonedwards2622]

Valid point. I like to pressure cook a jar of syringes with my liquid culture

[@C0Y0TE5]

That may be, but will the carbon buildup on the needle hinder the sealing of the injection port? Or does the use of a propane torch not cause carbon buildup on the needle? And the idea also was stated to cool the needle so the injection port does not get accidentally melted...

[@Magicsam87]

Can you not just suck the air back in infront of the flow hood to get sterile air?

[@RenegadeMushrooms]

Theoretically yes, but I've always been a fan of redundant safety systems. Even the best HEPAS don't catch EVERY contam and one bacterial endospore or trich spore in your LC and you're boned. I'm also a bit paranoid because my lab is a dusty old basement 😁

[@Rhizomorphius-vz4bj]

I noticed you equalized the pressure inside the jar then injected 10 CCs of LC going from negative pressure to a slight positive pressure inside the jar, meaning you put 10 CCs more air in the jar than you actually needed. If you need to introduce an estimated 30-40 CCs of air, why not start with injecting your 10 CCs of LC first, thus reducing the amount of air needed to equalize pressure by 10 CCs, and reducing your chances of contamination by doing so. It just makes more sense to put the LC in first then relieve the remaining vacuum.

[@RenegadeMushrooms]

Never measured it and it varies by jar but I would guess it's more like 100 - 200 ccs to initially equalize a pint LC jar. I usually only add 3 or 4 ccs from the syringe. If you injected a jar in a state of full vacuum it would rip a 10 cc syringe plunger down instantly. In theory I get what you're saying, but in practice once you do the initial equalization everything else works itself out in the wash.

[@MiigsMusic]

A (kinda obvious, but i forgot about it aswell) point about the RTV silicone ports...seems to work fine with his lids and the big diameter hole but mine were plastic (PF jars) with a hole half the size...should have put some sand paper to the bit around the hole...the Silicone didnt stick to the flat plastic at all and is just held in place by the silicone in the hole.

[@RenegadeMushrooms]

I've never had a problem with the high temp RTV sticking to the metal lids, but I can see that being an issue on plastic if you don't rough it up a bit.. Thank you for sharing your experience.

[@johnhall9378]

Thanks for the response. I have watched several of your videos and learned a lot. I was thinking about a hybrid system that has the advantages of the vented lids (no need to equalize the pressure with a sterilized syringe after the PC, etc.) , but also the advantages with non-vented, ( i.e. no thick mycelium, cultures last longer)

[@RenegadeMushrooms]

I find both are useful, so it's good to know how to make vented and non-vented LC.

[@bmartin2304]

I was thinking you could use a syringe filter to equalize, then use a syringe cap on the filter to stop the f.a.e. disadvantage to this is you are not eliminating any time or cost on the lids, and stacking sucks with syringe filters. Let me know if you came up with any good ideas!

[@seanvan6377]

Put an extra jar with a filter & 50ml water in the pc, draw from that one to equalize the non filtered jars, I have flat .2 micron paper filters for this & you dont even need to pc it again, everytime you pull with the syringe air is filtered on its way into the jar.

[@RenegadeMushrooms]

Cool trick 👍 This alcohol soaked polyfil deal is pretty quick and effective as well.

[@bg31337]

So even 18 gauge needles are not sufficient for the thick myc from a vented lid? 😳 I can't find any smaller gauges than 18 where I live

[@RenegadeMushrooms]

18 gauge works fine if you don't add the peptone to the LC recipe. It will grow fine with just the karo, just more slowly and not as thick.

[@loricox3833]

I am wondering at what temperature do you keep this culture at after until it is ready to use? We are keeping the cultures I made in a wine frig at 50-60 degrees

[@RenegadeMushrooms]

A wine fridge is a great choice actually for LC storage of cold tolerant species. Just remember that tropical species like pink oyster and almond agaricus should be stored at room temperature, as temps down in the 40s can harm or kill them, 65F is good for them.

[@JasonAlexzander1q47]

Will that work with a syringe full of spores? Inoculate a jar with a syringe full of spores?

[@RenegadeMushrooms]

Yes it works well with spores too as long as the spore syringe is clean and sterile.

[@paulskilling3879]

those jars did not get vacumed sealed. the injection port stopped it from happening. it is not nessasary to inject air into them. i am a canner of 22 years. any little opening will stop the vacumn from happening.

[@RenegadeMushrooms]

Well I respect your canning knowledge, but you should respect my mushroom growing experience as I've been running these jars in PCs as long as you've been canning, and you're incorrect. Those injection ports are self healing. That's how they keep contaminants in the air from getting in your jar, otherwise they would be useless. You honestly think I would go to all the trouble of making this video if it wasn't necessary 🤨

[@ginalogist]

Hi, interested in trying the step to let sterile air in, do you mind sharing a link to the needle used. I understand it’s heavy duty 12 bf needle? TIA

[@RenegadeMushrooms]

I'm actually using a 16 gauge BD needle that I got for free with a LC syringe.

[@mjarami3]

Would you use this method for a bigger jar? Or would the larger jars require more fresh air?

[@RenegadeMushrooms]

It works fine for quarts, never tried it for half gallons. I'm sure there is a point where the liquid volume would require a filter to myceliate sufficiently.

[@Rawabina22]

If you would be so kind, what are the components of the liquid culture that you prepared, in detail, each component separately .. youtube.com/@SalihNawman

[@RenegadeMushrooms]

Per jar: 600 ml. distilled water 25 ml. karo (corn) syrup .25 g. soy peptone The soy peptone is optional

[@Rawabina22]

@@RenegadeMushrooms Thank you very much for your cooperation. I did not see you putting mushroom spores with wheat grains. Are the components of the syringe replacing?

[@RenegadeMushrooms]

@@Rawabina22 Yes, the liquid contains living tissue of the fungus that will colonize the grain. No spores needed.

[@Rawabina22]

@@RenegadeMushrooms I appreciate your patience ، thanks a lot ..

[@mattnstewart262]

Could one stuff a synthetic filler disk in the syringe instead?

[@RenegadeMushrooms]

You could use a syringe filter, but you need to make sure everything downstream of the filter is clean as well. There are lots of ways to vent jars, this is just what I prefer, never let me down.

[@calichojack991]

Can I buy syringes in local stores or do I need to order them online?

[@RenegadeMushrooms]

Better to buy them online imo. This is where I get mine, they are an affiliate of ours: www.shroomsupply.com/syringes-needles/sterile-syringes/?referrer=CNWR_684691708647669

[@blablacar7886]

Couldn`t you just use a steril liquid to depressurize?

[@RenegadeMushrooms]

There's lots of different ways to do it, but it would take around 100 ml of fluid to de-pressurize my jars. This way is easier.

[@FlomatonFamous]

Good stuff, good info in your video's

[@kimelize]

How long can you keep a master jar going?

[@RenegadeMushrooms]

1 year or a bit more under refrigeration.

[@robertmenucci2668]

Can you reuse all syringes, from the same strain, to transfer LC? Like taking LC out to Vax substrate?

[@RenegadeMushrooms]

I use a syringe once, pulling in LC and then dispensing it into jars. IMO pulling more LC back in risks introducing contamination without resterilizing the syringe. Not saying you couldn't get away with it but I don't do it. Syringes can be washed, wrapped in foil or placed in a jar, and re-sterilized in the PC though. I also use 20cc syringes when doing transfers so I get a little more volume. Syringes with LC left in them can be stored for a couple months in a ziploc bag.

[@robertmenucci2668]

Thanks for the reply. I'm a big fan of the show. Being new at this, I have to "pick my battles" to save money, and I'm not willing to risk an entire batch in order to save a few bucks

[@myotheraccount5947]

I see a lot of videos where guys are using magnetic stirrers to help the mycelium grown evenly throughout the liquid. Not necessary?

[@RenegadeMushrooms]

Not necessary, but a very nice tool. I've never bought one because I've never really found I needed it, but they are cool.

[@myotheraccount5947]

@@RenegadeMushrooms Thank you!

[@JohnLeggio]

Hi! would the nooks and crannies on the red silicone injection ports make it difficult for the alcohol to do its thing? The stick on ports seem less hospitable to unwanted guests

[@RenegadeMushrooms]

No, it's never been an issue for me but when you apply your RTV you do want to make it as smooth as you can. Less nooks and crannies vs. more.

[@JohnLeggio]

​​@@RenegadeMushroomsFor sure! You have a ton of videos and its clear you enjoy making these... It would be awesome if you put out a blooper reel one of these days

[@Beyondbuttonmushrooms1738]

Where did you manage to find a 12 Gauge syringe tip? Im not having a lot of luck.

[@RenegadeMushrooms]

They're hard to find nowadays. Shroom Supply has 14 gauge needles the work great though.

[@Beyondbuttonmushrooms1738]

@@RenegadeMushrooms I wonder why this is the case. Is preferred amongst drug abusers? 🤔

[@AlexanderSchwarz-ck3cx]

When you put the squeezing thing out of the syringe, isnt contaminated air going inside?

[@RenegadeMushrooms]

@@AlexanderSchwarz-ck3cx No, any air that flows through the syringe into the jar is filtered by the isopropyl soaked polyfil inside the syringe.

[@AlexanderSchwarz-ck3cx]

@@RenegadeMushrooms ahh and its automatically flowing, because there is a vacuum inside the jars. Thank you for the quick Response, I definitely subscribe!

[@bqoutdoors2334]

When you pressure cook the jars do you tighten the lids? Also do you turn the lids upside down? Thanks I subbed

[@RenegadeMushrooms]

I snug the lids down tight and they are the right way, not upside down. I've never had one explode on me, but I only fill them to 300 ml in a pint. If the jars were full of liquid, they might break on you.

[@jeremyprince4945]

Do u have to use the soy peptone?

[@RenegadeMushrooms]

No, it's a nice supplement. But just karo will work.

[@kevinabood3598]

thank you for this i was searching for how to make a diy injection port

[@ChristophHalverson-iw8gk]

I’ve had a bit of bad luck with my LC coming out of the PC looking like a dark burnt mess, any tips?

[@RenegadeMushrooms]

How long are you cooking it in the PC? It only needs 30 minutes at 15 psi. Usually really dark color is cause by sugar caramelization from overcooking.

[@ChristophHalverson-iw8gk]

@@RenegadeMushrooms I’ve done it a few times and probably cooked it a bit too long, but I was also thinking perhaps I didn’t have it mixed well enough. It comes out all settled to the bottom and clearly burnt

[@RenegadeMushrooms]

@@ChristophHalverson-iw8gk What recipe are you using, mine?