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...Plantain sucker multiplication/Macro propagation Introduction...
Tissue culture is the artificial multiplication of plant tissue or cells outside the parent plant.
Main Aim/objectives
-To replicate one sucker into 50-64suckers(peepers)
-To produce a clean and healthy sucker free from nematodes within 2 to 4 months.
Materials Needed
*plantain sucker
*saw dust
*water source
*watering can
*nematicide
*cutlass
*knife
*Rake
*Hand gloves
Procedure/Method
1.Remove a maiden sucker from the parent plant
2.Remove the outer layer using a knife.
3.cut the sword
4.Cross-cut the base containing the apical meristem into 4 equal parts ÷ each portion into two making 8 portions.
5.wash the 8cuttings in a solution of nematicide to get rid of nematodes
6.Make a bed and spread sawdust on it & sow the cuttings
7. Cover them with sawdust to facilitate germination & water depending on the soil moisture conditions.
8.sprouting of peepers(small suckers emerging from the soil) starts after 21 days.
9.Prick/remove the peepers & transplant them at the post nursery where they continue to grow for four months before they are transplanted on the main field.
Note After 21 days , each cut replicates 8 peepers. which means , 8*8=*64suckers*can be obtained from just one sucker through tissue culture.