How to See Sperm at Home Under a Microscope

[@micro_safari]

CORRECTION: In the video I stated that it is not worth trying to count sperm at home. After digging more into the WHO Sperm Analysis Document, there is in fact a procedure to estimate sperm count that is straightforward and does not require specialized counting chambers. This procedure can definitely be done at home. I am writing a blog article describing how to do this now, and will release a video on it in the future.

[@user-th7gd7ge4p]

could you please make a step by step tutorial on how to obtain the samples

[@onna8908]

💀🤣

[@andreasobuaculla9511]

Exactly,first hand experience

[@aidantrojan3710]

I’d like to see the footage of the sample being collected…. For science of course.

[@user-nw6jq7tu9n]

Lol😂

[@marivega3294]

😂😂😂😂

[@liviu8101]

you can also use dark field filters

[@micro_safari]

Agreed! Darkfield filters also work, however I've found that because the specimens do not strongly scatter light, you need a very bright transmitted light source to make this work. A 3W LED that is found on most consumer microscopes is too dim to get a meaningful image. Also the heads will show up much brighter than the flagella, and so if you are using a camera to image the specimens it is hard to have both the head and flagella properly exposed without blowing out the image. Advice to anybody trying to use darkfield to image sperm - use a darkfield filter with an NA stop just above the NA of the objective so that you are maximizing the oblique light that hits the sample. If you are using a camera you may need to manually adjust the exposure to account for the dark sample.

[@ExioElioCabreja]

I am a new beginner in the Biologist studies to become a Degree biologist in (4) four years,i have purchased a telescope 40x - 1000x just to get to know biology more oneday i am going to purchase this one you are introducing just to use it for soil cool kit just watching feels like we are in a Hand-on-hands lab.

[@MosesOtumi-i5k]

I am a beginner in biologist please help me in some topics

[@MosesOtumi-i5k]

I will like to see a sample being collected

[@Niru_24105]

Staining not required? I have a general microscope, can i observe it?

[@kapa1611]

you can. it works even in a cheap (>150€) microscope, that has 4x, 10x and 40x magnification. i'd recommend 10x

[@ronnykhalil]

my new favorite channel. can't wait to start playing with the Horizons bundle of wonders

[@micro_safari]

Woo Hoo! Thank you Ronny, we are so excited for you to receive and start using your kit!

[@neilsullada385]

This dude really knows how to prepare his edge detection on a slide

[@iulianbina7910]

Does the kit include an onlyfans subscription or is it the old school porn magazine?

[@E85_STI]

I have an Amazon pocket microscope but idk if it’s strong enough to view something like this.

[@randomphile3663]

did you try?

[@micro_safari]

A phone microscope will not have a high enough magnification nor the necessary illumination setup to observe sperm. See the what microscopes can see sperm video for more details

[@ortegami01]

4:41 ""pretty easy to tell what sperm is and what's not sperm.." in the foreground we see a sperm.. but in the backgroud we see round cells moving.. are those also sperm out of focus?

[@micro_safari]

Good question! No, those are likely just particles that are moving due to brownian motion (not necessarily alive). Sperm cells should all be roughly the same size and, unless they are greatly malformed, will have a flagella (the tail).

[@Eragarev]

Are these yours, or are they from a donor?

[@HejaSverige]

why would he use someone else’s when he can use his own

[@soorpilfishing]

Can you suggest me a better microscope to give a gift to my daughter....

[@micro_safari]

Sure thing. I agree, a sperm kit is probably not the ideal gift for your daughter! I don't know what your budget or age of your daughter is but I'll throw out the Carson Microbrite has been a favorite of ours recently. Check out my general microscope recommendation videos or you can wait for a couple of weeks when we release our 2024 recommendations

[@wokeisnonsense]

They are not swimming. How come?

[@micro_safari]

It could be a couple of reasons: 1. Our filming studio is in a warehouse that gets quite cold in the winters. I remember it being about 40degF that day. Not the ideal environment for sperm to be the most active! 2. When you do a sperm count test in a lab, they have you wait 7 days since your last ejaculation, which is when your sperm count/count of motile sperm will be approximately the highest (and for consistency between patients). Let's just say that we were filming back to back to back sperm related videos that day and the days prior, and this was the last video we filmed ;)

[@tomstur119]

What camera are you using?

[@micro_safari]

It's the USB eyepiece camera that we include in the Sperm Observation Kit and in the Horizons Lite Kit. It happens to have really good edge detection which is why we included it in the Sperm Kit. Basically any microscope camera will work better for this than your eye by themselves. If you are looking to build out a kit to view sperm specifically, I highly recommend you include a camera of some kind in your setup. Here's a link to the camera in the video: microsafari.org/collections/shop-all/products/usb-2-0-cmos-digital-eyepiece-microscope-camera

[@j.olacio5298]

I purchased a AmScope to look at dog semen and I can't see anything. It has a WF25X eye piece and 100/1.25, 40/0.65, 10/0.25, and 4/0.10 lens. Sorry, I don't the technical terms. Should this microscope work for this? Or am I probably doing something wrong?

[@kapa1611]

0:19 just think about science 🤣

[@fabianeamorim1023]

I have 200-4000X microscope can sperm been seen using this type?

[@micro_safari]

It really depends on what the illumination method is more than the magnification. I made a blog article that should answer your question: microsafari.org/pages/what-kind-of-microscope-you-need-to-see-sperm

[@randomphile3663]

Why they are not swimming?

[@micro_safari]

Could be a couple of reasons. The process of collecting and micropipetting the sample can sometimes kill the sperm cells. The office that I was doing this in was also really cold that day. I think in this sample I also opened up on a small bubble at first so the sperm were out of the liquid.

[@E85_STI]

Would dropping the lens on the drop kill them?

[@uhoh5473]

@@E85_STIif it hits them then yes

[@Pw614]

Whose sample is it? 👀🤢

[@justinjjoachin448]

I wish you could tell us how you got this sample.

[@DrDixonBudtzMD]

😏👉👈

[@fabianeamorim1023]

if you dont see them moving what that mean?

[@micro_safari]

Depends on how many aren't moving. According to the World Health Organization, men can have up to 65% of the total sperm be not motile and still be considered "fertile". The assessing sperm motility section of the blog article I wrote will have your answers. It is most of the way down the page. microsafari.org/pages/the-comprehensive-diy-guide-to-assessing-male-fertility-at-home

[@mysteryiro1101]

I'm eating a sandwich watching this nasty sht.... .I'm feel scared a little bit... not gonna lie..

[@Handl949]

They seem dead 😵