HPLC | High performance liquid chromatography

[@artemiskar1897]

youtube once again helping more than the university instructors

[@shubhp003]

KZbin is ❤️

[@ritutak7997]

Yes

[@shiftyjesusfish]

Your absolutely 💯 correct

[@yogitashirsat5969]

Exactly!!

[@naveenb9515]

O yeah

[@ArtuursPaulausks]

Well, as a guy who daily works with HPLC have to say video is very good for brief introduction. Obviously HPLC is more complicated, with much more details. Just want to give shout out to all chemistry students and tell them to not be afraid of HPLC. In most cases, it is easy to develope a method on HPLC to determine some kind of compound, it might take just a few tries to make sure your compound of interest is separated from rest. Just always remember to filter your samples using 0.45 um filters and inject clean samples, also use guard columns :)

[@TrangNguyen-nw9dq]

Dear, It is grateful when I read your comment. Could you suggest some videos which relate to developing method analysis on HPLC. I am a student who is learning HPLC to support for Master's and phD courses. Therefore, I appreciate if you could support me. Thank you so much.

[@animaluveteranu9523]

don t forget to mesure you r standards corectly and shake them on ultrasonyc device It happends to my colegue and he finds out after 3 days XDDDD but I have a question for you if you work on hplc are you working on spectofotometers that use uv light?

[@ArtuursPaulausks]

@@animaluveteranu9523 UV/VIS thermo Dionex Ultimte

[@冯玲巧]

Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

[@helmetongrass1893]

hey, uhh.... can you please tell me if he said absorbent or adsorbent material? I mean when he is talking about the material in the column... 1:34 Edit: Nevermind, it's adsorbent material

[@nbent4607]

Real-life heroes don't wear capes, a man once said. Thanks a lot!

[@cioccolatamania3622]

Thanks!! I am truly amazed by chemists and engineers that develop these analytical instruments

[@srenathganesan]

Awesome explanation. No need to search for any other topic. Its a complete combo package. Thank you very much for making such a video.....

[@CM-yp2hg]

Here cuz I lied on my resume

[@haven3911]

How’d it go?

[@CM-yp2hg]

@@haven3911 It actually went really well. It’s not that I have no idea what an hplc is but I was not proficient enough to put it down in my resume by any means. I didn’t get that particular job but I applied to a few other and within a couple of weeks I became an analytical chemist and I am very well proficient in it now :)

[@sinchanakr6156]

Hey can you help me

[@NavjotSingh-uq7tc]

Me as well

[@akezelb7166]

Fake it until you make it 😊.

[@rachmadamayanti1858]

Wow, a really great explanation! If this video was launched during my college time six years ago, I would have been a great Chemist who confident about using HPLC.

[@冯玲巧]

Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

[@akezelb7166]

I am chemist with 7 year experience in Chromatography and this course is very useful simple clear and straightforward. Good job 👍

[@pharmapreparations]

In which organization you work

[@daanstrik4293]

Currently working on my internship report and needed a quick refresher. This video has been one of the most time efficient summaries i've found. Cheers!

[@冯玲巧]

Hi there, your latest post about hplc really caught our attention. We are the manufacturer of hplc and have 20 years experience, if you are interested, provide an email address and we can send you the case study proposal.

[@shinchanshinchan9155]

Thank you so much sir it's really a clearcut explanation i can't believe this almost a huge explanation is covered in 7mins 36sec

[@wiggywoman5184]

thank you for this video! i'm in a biochem grad program but coincidentally never took an actual biochem in undergrad, so these videos are helping a lot!

[@Richard-kz1ee]

This video is a life saver. Thank you very much . Importantly, I have subscribed to this channel.

[@mangosorbet8183]

This KZbin helps me more than my teacher in the college.

[@claudiosaitta3124]

Thank you very very much! after many years of study I finally can say I really, deeply understand HPLC

[@cozmos3678]

Brief and clear explanation... Thank u❤️

[@celebritystylewatch]

Explaining amazingly giving maximum concepts

[@கவிநிலா]

Thank you so much..With in a short time you've explained all the basic things in a wonderful manner with the help of drawings.

[@baskarang7618]

Within 2 hrs I have my xam. It's a 💎 for last min preparation . ..

[@anamariatorres6917]

The best explanation of HPLC. Thank you so much

[@aryanshah1943]

perfect explanation, thank you!

[@iroshasanjeewani6080]

Absolutely ,This was helped me to undestand about HPLC.Thank you ❤.

[@hope2633cu]

Awesome really we are learning a lot freely. Thank you so much

[@LinitedfiA]

Lmao. I still did’t get it why my class need 8hour to explain HPLC instead of 10 min. Good work btw really appreciate your work ,very helpful for overview.

[@chemvibes28]

It's truly very helpful to me. I am learning from this just before my exam. Lot's of love from India ❤🇮🇳

[@pharmapreparations]

India

[@jhansilanka1869]

Excellent explanation i undertood very easily

[@DawidFRF]

My phone fell off and that's why I'm here, I've always been curious how the experiment I had in my physics class worked 🥰🥰 thanks for that

[@setupatel1476]

Back ground music is not needed ,,it disturb the understanding

[@livesteps3384]

described very well.Understood the content clearly.Thank you very much.Plz do more clear lessons.

[@iddah3732]

Very well explained. I can now answer any question in HPLC with ease.thank you

[@naveenb9515]

O yeah

[@Hozanaa]

Thanks, it was very clearly explained and specific. Neat diagrams and good presentation. Easy and not messy ❤

[@sashansachinda9812]

superb clearly understand

[@ELICE6]

Great video and very simply explained.Thank you so much!

[@42najibshaikh98]

It's just awesome. Shorter video , better explanation.

Thanks for this amazing video! Everything was clear!! 🎉❤

[@ulamgnuszewska]

now I understand! You're a great teacher :)

[@pharmapreparations]

Nice

[@alphynisasteaphen282]

thank you for such excellent explanation

[@vig7095]

Once again, an Indian guy saving my grades 2 days before my Chemistry Test.

[@amrayyad5897]

here with a typical night of due paper in post-grad after not knowing anything about chemistry for the last 3 years

[@shivanshsingh748]

reconsider your chosen field maybe.....

[@naveenb9515]

Haha

[@drashtidarji]

Amazingly explained!! 😍👍

[@anikislam8507]

love this channel. from bangladesh.

[@shalinithakur3323]

Very good......Other wise, in😮 first look HPLC seems to be quite difficult. But first understand this video....... the rest of this become simple.

[@militarybrat123]

Osmm it's enough for exam . 🔥

[@sabinahasanova7198]

SO SIMPLE AND INFORMATIVE! THANK YOU

[@TechsScience]

Very well explained 👏 Thanks for this video

[@sirtintly390]

This was just randomly recommended to me. I don't even know what this is.

[@nk_17]

Lol🤣🤣🤣 I wish this was recommended to me earlier ...😂

[@MB-vd6hc]

that was awesome, thanks sir.

[@AS-zf4nj]

You're the real MVP.

[@MonkClasher]

Nice. It's easy & simple to understand ❤

[@christophergoudarzi9992]

Great video as always, thanks so much!

[@aprajita629]

Thank you so much. Very nicely explained with animation & way u speak. Plz keep it up.👍👍👍

[@dilshanchathuranga6962]

Thank you very much . This video was very useful for my exam❤️

[@roroiyaka]

very helpful, thank you for making the video. much appreciated

[@chennamsettiprasad7257]

What ye class explanation,,great video

[@NannannanSJN]

Thanks for video. It helped me to understand HPLC!!

[@tanvirahsan5775]

Outstanding, Thank you ♥️

[@mahakrathod8679]

Best video on HPLC

[@shirinmittal3225]

Very nice explanation. Thank you

[@pepelaugh5825]

Really really thank you for the video The very best explanation

[@pharmaboy1510]

Love this Channel From Bangladesh

[@varunkibett5955]

Informative piece 😊

[@jacktam5855]

You saved my life. Thanks man

[@rowidakhalily5760]

Awesome explanation,Thank you so much

[@AA-gl1dr]

Excellent video, thanks!

[@DeniseP]

Size exclusion 5:55 Ion Exchange 6:22

[@iannalins2369]

this video helped me so much for a apresentation, thank you so much!!!

[@btsforever5123]

Tq so much sir it's easy to exam preparation😇

[@Ocyon]

Good video! Thanks for that :) Cheers from Germany

[@shabinmillet9261]

explained very clearly and nicely

[@Serwada237]

The best lecture here

[@ahmadhamza6618]

Best vedio to understand hplc

[@Lia_liah]

Thank you for the vedio and by this vedio i understood the topic very clearly

[@priyankapriyadarshini8308]

It was very much helpful 🤩🥳🥳

[@anchorsreyashi]

Very nice explanation...thank you

[@scimathlab197]

tHANK YOU SO MUCH FOR THIS SHORT INFO!! SO USEFUL AND HELPFUL!BINGO!

[@Vaibhav............]

Best explained ever tysm♥️♥️♥️

[@samuelbabalola3479]

This was super helpful!

[@martinnrrekjrgrndahl3715]

Nicely done introduction to the subject. Feel a little more confident next time I am applying for a job mentioning this technique.

[@nk_17]

You got the job?

[@romulo7961]

Curso terrível que eu escolhi pra minha vida

[@ahmadfares5243]

Simple and clear thank you 😊

[@esraaalmasoudy4630]

This was helpful. Thank you sir.

[@gandhamgracy4937]

Easy to understand 👍🏻

[@MayankSingh-uj6hi]

Really awesome videos

[@myworldofinstrumentation6523]

Helpful information

[@shaikabdullah..]

Good explanation

[@shivaramsmjadhav3298]

Love u & thanks ur making very good vedio ....I'm ur new subscriber from India biochemistry student

[@vsilvia1906]

I love your videos thank you so much!!!

[@Abdulkader-sq3qo]

Very well described.

[@nurilazizah260]

Thank you very much. nice explanation :)

[@_archana_4147]

Superb!!! 🙌

[@HarukaKaku-l7p]

I like this video so much. If you don’t mind, I’d like to ask your permission to share this video to the other website in China for the embarrassing reason that KZbin is blocked form accessing in China. Of course, I’ll give sources of the original website. Thank you so much.

[@samueld77]

Great video

[@hafizghaniullahsafi6853]

Well explained 🧡

[@hussainsaleh389]

Very great explanation my dear

[@casper2917]

Thanks for the explanation

[@enumahchinweoke127]

Explain more on the polar and non polar and what they got to do with the phases? Thank you!!

[@michaelberman3648]

Briefly, a more polar molecule indicates that there is a net negative charge on one side of the molecule and a net positive charge on the other side of the same molecule. And the net negative and positive charges are dispersed over a specific region location of the molecule. But keep in mind that the more "polar" a molecule is, the greater the separation of positive and negative charges exist on the same molecule. For example, a non polar molecule would be carbon dioxide or benzene because there is complete symmetry because carbon dioxide is not bent but is straight and the regional negative charges on the two oxygen atoms connected to the carbon atom cancel each other out to form a net zero dispersed negative charge. Likewise, the benzene molecule is flat and symmetrical as well. On the other hand, water is a bent molecule so the dispersed net negative charges on the two oxygen atoms result in a net negative dispersed charge on one side of the molecule and because the electron negative charges migrate more towards the two oxygen atoms, the result of this is that there is a net positive charge on the other side of the molecule where the hydrogen atom is located. Thus water is a very polar molecule. And also remember that in all forms of chromatography, one should always keep in mind that "like dissolves like". Meaning that in general, a polar solvent will dissolve a polar solute or molecule of interest to analyze. And a non-polar solvent will dissolve a non-polar solute. For example, all salts are polar so a polar solvent such as water will dissolve a salt which is a polar solute because salts have a distinct positive charge on one side of the molecule and it has a distinct negative charge on the other side of the salt molecule. On the other hand, a non-polar solvent liquid such as benzene will dissolve a non-polar solute molecule such as carbon tetrachloride, hexane and many other relatively non-polar solutes as well.

[@kdhasdgv]

@@michaelberman3648 Thank you so much

[@claudiaatsu1056]

That is a very good explanation! Thank you, is there any paper that you suggest that explains the function of HLPC? In order to detect vitamin K with the technique of HLPC, is there only that procedure or is a combine procedure with another technique? Thanks

[@cheneecleveland4588]

Very helpful review!

[@abdulrahman-ob5wx]

Thank you soo much for helping us

[@EnglishExcel2208]

Nice xplanation

[@narmeenfatima5145]

thanks alottt it was very helpful