I Opened a Sealed Jar of River Water After 1 Year

What happens when you open up a jar of sealed river water after 1 year?
The smell was, well, unexpected.
The pressure in the jar was also surprising and was not what I expected at all!
This closed ecosystem, one of many I've put together, is an amazing testament to the resilience of life in extreme environments, and also is a gross reminder of what lives in the water of the river or lake that you may have grown up swimming in each summer, or maybe still swim in today!
Also, upon closer inspection, I'm pretty sure those are copepods, not daphnia, though there could be both swimming around
Jars I like to use for these aquatic ecosystems: amzn.to/46WSuN9
Camera I use: amzn.to/3DmOGqN
Macro lens I use: amzn.to/3Dl3oP6
The above links are affiliate links for which I may be compensated for.

[@Cwrigh25]

DUDE. I swear you are going to get a viral video and blow up. This reminds me of old youtube. Just a guy with a camera showing us a passion we never knew about.

[@GlassOasis]

Thanks! Just published a new one a minute if you want to check it out!

[@Cwrigh25]

@@GlassOasis I saw it right after I posted this comment, gave it a full watch and a like.

[@GlassOasis]

Thanks! Trying to up the quality with each video, so to anyone else reading this, check out some of the other videos once you're done with this one!

[@Bigonewsnetwork808]

ur right man , but its the money that messed it up for sure just like gaming girls want the money and attention so bam now its tiity streamers fake game players like sniperwolf .

[@MissusFroggy333]

I agree. It's bound to happen eventually; these videos are mesmerizing 🤩 I just subbed, time to binge watch the rest 😄

[@XtayCalmEco]

You can tell this is one of those channels that is going to have over tens of thousands of subscribers if the effort is kept up. Freshwater ecologist and closed system enthusiast here, looking forward to your next video and get in touch if you ever feel the need!

[@GlassOasis]

Thanks for the encouragement! The videos take a bit of work for sure, but it's something I enjoy a lot, even if my knowledge base is still catching up to people like yourself.

[@jshaw4757]

​@@GlassOasisJust subbed from U.K good luck with channel 👍

[@comicspace8034]

@@GlassOasis I also just subbed from the US! Your channel could have up to 400k in the next year if you keep it up!

[@treboris]

My theory on the pressure, the seal acted like a one way valve. During summer months when the air expands it seeps out but isn't able to suck back in when the air cools. I've seen military style containers that seal this way. They have a special relief valve and have to be priced apart if it fails.

[@Reptileowner]

I would have just assumed that it was sealed during low pressure and opened during high pressures

[@CMZneu]

4:12 Aside from a ton of natural chemical reactions going on in the jar over the course of one year, the simplest and most probable reason for low pressure inside would be it was a warm day when you closed it and a colder day when you opened it.

[@jasmith1867]

I hope you returned the little jar full of life back to the river. They deserve a reward for entertaining us.

[@osamabentrappin4102]

I agreee 🙋🏾‍♂️

[@Teenywing]

Me too❤

[@jasmith1867]

But if he just flushed them they might be even better off living off the sewer chow line.@@Teenywing

[@niccolashood5266]

If I had to decide on a reason for the low pressure, it has more to do with the rate of decomposition vs the rate of growth/photosynthesis. More than likely there were more things alive when the jar was first sealed than now and decomposition, specifically anaerobic decomposition from a low oxygen environment is known to reduce overall volume, which can be a reason for the negative pressure.

[@grelm1322]

At first, when the music was getting louder I was like "wtf?" But then your voice started getting louder and it made me lol. Thanks for the laugh man. Very cool presentation btw

[@GlassOasis]

Glad it made you laugh!

[@osamabentrappin4102]

For real it got me bro🎉

[@666pinkster]

The pressure in the jar probably stayed exactly the same, but the air pressure in the room where you had it stored was probably vastly different from when you had originally stored it. Ambient air pressure changes constantly, the sealed jar would be as it was when sealed.

[@uncletiggermclaren7592]

You know the day you sealed it, and the day you opened it. So you could look for the ambient air pressure of those days on a weather site, to see if maybe it was low pressure weather when you sealed it, and high pressure when you opened it. It makes a considerable difference.

[@carlsmith4568]

Nice insight

[@AnAverageArsonist]

I have a pretty good feeling about this channel, i feel like this is gonna be one of those small channels that will suddenly explode in popularity if they keep making this type of content. I'll try to stick around and see how much this channel has grown EXACTLY 1 YEAR from now. So far, only 525 Subscribers

[@GlassOasis]

Thanks, this kind of encouragement is so great

[@truenightsedge4163]

Reply to this comment with the answer so I can see it

[@bleachtea1686]

@@truenightsedge4163he’s tripled in two weeks

[@aj5988]

Oh wow! The quality of this video and narration I would have assumed you had hundreds of thousands of subscribers. I go and look and see you've only just cracked 2k. 😮 Congrats on the 2k. This channel will go far. Loved the video.

[@GlassOasis]

Thanks for the kind words!

[@oskar9090]

I am so glad that I have found this channel now. Keep up the good work

[@geronimomiles312]

Gasses are locked up as organic molecules in plants etc so you end up with a low pressure , as opposed to decomposition where the gasses are liberated. The light drove a net increase in biomass.

[@houyeet]

Very interesting, and the shots look incredible. Keep it up, you’re good at what you do

[@mariofilippi3539]

I've been collecting water from my pond and rain barrel, placing it in a jar exactly like yours. Lining the bottom with small stones encourages those critters who hide and live at the bottom. Thank you for the video.

[@Wildstar40]

The jar was warmer when you sealed it than it was when you opened it thus explaining the vacuum. When you are canning food it goes in the jar hot then sealed. When the jar cools a vacuum builds up thus contributing to long lasting canned foods.

[@quintonharvey1957]

lol love your sense of humor. This is my first video but you've got my sub as long as your manager keeps you from holding the suspense tooo long :p

[@GlassOasis]

He's a real pain, let me tell you

[@ughhkai]

This is interesting. I can tell this channel will explode and boom in popularity one day! Keep up the good work.

[@GlassOasis]

Thanks for the encouragement!

[@jayklink851]

Brilliant video man, it was absolutely fascinating!

[@GlassOasis]

Glad you enjoyed it!

[@pchelloo]

Beautiful nature; if only humans could live in harmony with it.

[@MentalSqueeze]

This is so interesting, I would love a more in depth analysis of the different species and how the overall ecosystem is able to survive while enclosed

[@GlassOasis]

Definitely, it's so much more than just photosynthesis and energy production, even if those are some of the more important basics of making the system work.

[@yoshi_wrld4892]

Repent of sin and Trust in Jesus! John 14:6 6 Jesus saith unto him, I am the way, the truth, and the life: no man cometh unto the Father, but by me. Matthew 7:13-23 13 Enter ye in at the strait gate: for wide is the gate, and broad is the way, that leadeth to destruction, and many there be which go in thereat: 14 Because strait is the gate, and narrow is the way, which leadeth unto life, and few there be that find it. 15 Beware of false prophets, which come to you in sheep's clothing, but inwardly they are ravening wolves. 16 Ye shall know them by their fruits. Do men gather grapes of thorns, or figs of thistles? 17 Even so every good tree bringeth forth good fruit; but a corrupt tree bringeth forth evil fruit. 18 A good tree cannot bring forth evil fruit, neither can a corrupt tree bring forth good fruit. 19 Every tree that bringeth not forth good fruit is hewn down, and cast into the fire. 20 Wherefore by their fruits ye shall know them. 21 Not every one that saith unto me, Lord, Lord, shall enter into the kingdom of heaven; but he that doeth the will of my Father which is in heaven. 22 Many will say to me in that day, Lord, Lord, have we not prophesied in thy name? and in thy name have cast out devils? and in thy name done many wonderful works? 23 And then will I profess unto them, I never knew you: depart from me, ye that work iniquity. John 3:16-21 16 For God so loved the world, that he gave his only begotten Son, that whosoever believeth in him should not perish, but have everlasting life. 17 For God sent not his Son into the world to condemn the world; but that the world through him might be saved. 18 He that believeth on him is not condemned: but he that believeth not is condemned already, because he hath not believed in the name of the only begotten Son of God. 19 And this is the condemnation, that light is come into the world, and men loved darkness rather than light, because their deeds were evil. 20 For every one that doeth evil hateth the light, neither cometh to the light, lest his deeds should be reproved. 21 But he that doeth truth cometh to the light, that his deeds may be made manifest, that they are wrought in God.

[@lorenzoblum868]

​@@GlassOasis each single detail is relevant. Including the reason why low pressure. More than just one explanation...

[@j0hnny_R3db34rd]

Wow, this is fascinating! It's incredible how nature works. The low pressure vacuum that formed in the jar after being sealed for a year can be attributed to several factors. One possible explanation is the cooling and contraction of air inside the jar as it cooled down over time. As the warm air inside cooled, it lost energy and volume, creating a partial vacuum. Additionally, any microorganisms or organic matter present in the water may have consumed oxygen during decomposition, causing a decrease in gas pressure within the jar. This combination of factors likely resulted in the formation of a low-pressure environment inside the sealed jar. Truly amazing how our environment continuously surprises us!

[@GlassOasis]

I agree, definitely could have been any of those one reasons, but probably a combination

[@bopemuworty3163]

dude! nice work, it will take allot of time but you can totally continue to make great content like this and draw a audience

[@GlassOasis]

Thanks!

[@darianroscoe1017]

Look at all that life...I learned two things. I love this video, and I'm never setting food in a river again. New subscriber.

[@GlassOasis]

Welcome aboard!

[@MrIgottap]

“Setting FOOD in the river ever again”?………dang you autocorrect, I’m about sick of your shirt, lol

[@Youtubecensoredmyusername]

@@MrIgottapto cool it down. I throw my drinks in the pool for this reason

[@MrIgottap]

@@KZbincensoredmyusername woooooosh!

[@grace7961]

Wow! This video took a lot of effort and time! Nice job!

[@GlassOasis]

Thanks!

[@natashad9261]

The dance music omg lmao❤😂😂 love it so cool!!

[@Theguyofunsucessful7180]

How did you got only subscriber around 900? You deserve more than 10k, it's impossible that this quality video have less than 100k views, i hope this channel blows up one day with this epic quality.

[@GlassOasis]

Thanks!

[@chaosopher23]

The life forms in the jar consumed most of the oxygen, and then balanced out as a closed ecosystem with what consumes CO2. I wonder how long that could have gone on?

[@cmdrbraus4176]

I'm gonna make my kid watch this. This is something we can do at the house and she can watch the life sustain itself. Keep it up buddy. Subbed.

[@GlassOasis]

Thanks man. I've got two you girls, and the youngest of them know way more but names then I ever did at 4.

[@cmdrbraus4176]

@@GlassOasis Keep sending it buddy.

[@nick68231]

Besides the outside air pressure when sealed and when opened and temperature changes having effects as most people point out. Another possibility is that the organisms inside had a net negative influence on the gas pressure in the jar over time rather than a net positive influence. In other words they used more of the gas for their own biological processes than were produced, thus creating a vacuum.

[@jeremyshearer]

I love these videos, there’s comedy to just like the loud music part lol. I subbed :)

[@user-cy8ly3eb3m]

This is one of my favorite KZbinr's now

[@guillaumelafleche9477]

The negative pressure must be from plants converting gas (CO2, H2O) to solid (sugar, starch, sediment) and/or a drop in temperature.

[@shashankraul]

Reason behind vacuum is when you leave your jar in hot conditions like sunlight etc water and air in the jar gets expands and pushes the air through the rubber sealing but when temp😢 gets normal air won’t go inside again. I have observed this in my terrarium. Water leaks through rubber sealing in high temperature conditions.

[@humanbean3]

"am i looking at the before or after" is what i was wondering throughout the entire first 4ish minutes

[@de4md686]

400th Subscriber! woot! Keep it up man. Great video! great quality

[@GlassOasis]

Thanks so much!

[@christinejacobs71]

I’ve got a jar that I took from my garden pond nearly two years ago that still has life in!

[@GlassOasis]

That's awesome!

[@tonyquark493]

My theory on the low pressure when you opened the jar: Originally, there was 14.7 lbs-per-square-inch of atmosphere in there. When it was sealed up, the existing oxygen was slowly used up, but because you have oxygen-producing plants and organisms in there, they generated more. Not the same pressure as sea level obviously, hence the partial "vacuum." But enough to sustain life. I'm sure the nitrogen was also partially used by whatever is in there and perhaps re-generated partially through the organism's waste. Basically it's own little eco-system. Amazing!

[@FischerNilsA]

Lotta ways some gas component could have been used up by plants or animals living. Absorbed, chemically bound. Could also be it was a good deal warmer when closed...... I get pops like that when I filled glasses with hot jam or sauce. Where nothing (hopefully) is alive.

[@kjay5587]

My best guess for the negative pressure is that the water may have been warmer, so Containing less dissolved gasses. When it cooled more gasses would dissolve from the trapped air. Having gone into the water, that would make the negative pressure when you open now.

[@privatepilot4064]

The day you sealed it you should have recorded the ambient barometric pressure and compare it to what the barometric pressure was on the day you opened it. And the temperature of the contents of the jar. Excellent video. Plants are natures way of maintaining and regulating the proper CO2 levels on planet Earth. The more CO2 we create the more plants will flourish. The less CO2 we create the less plants will flourish. It’s really a no brainer.

[@GlassOasis]

Great suggestion!

[@ms.sharpe8820]

I think it didn’t build up pressure bcz there was already so much bacteria in the water. Usually I see pressure built up when bacteria forms in a liquid where there is very little bacteria to begin with.

[@TheHappyflea]

Crawly soil critters, slime, death, decomposition and renewal. Better than Netflix.

[@SparkyBeer]

Pressure and temperature directly correlate together, I'd say most likely when you fill the jar it was warmer than when you open the jar. Lower temperatures cause lower pressures.

[@nahiyanalamgir7056]

I think when you sealed up the container, there was a specific volume of gas on top. However, this gas (oxygen, nitrogen, etc.) got used up (to an extent) by the ecosystem and turned (partially) into solid waste (probably the gunk you saw on the top). This conversion would result in lower pressure inside the jar.

[@denisyogore9660]

Interesting stuff going on in that jar! Loved your video! You got a sub from me, hope your channel explodes man!🤙😊

[@GlassOasis]

Thank you so much!

[@palpaladin315]

Its called vacuum pressure. - a space can be filled, or it can be vacant; both can be measured.

[@stacyevans1360]

What was the atmospheric pressure when the jar was sealed? If it was low and there is a high pressure system in when you opened it then it would be negative pressure in the jar. For more accurate testing you would unseal the jar at the same pressure.

[@MrJayrock620]

It kinda make you wonder what could be living in complete isolation 1000’s of feet underground in untouched aquifers

[@GlassOasis]

Definitely. I just saw an article about a new millipede species that was just discovered underneath Los Angeles.

[@BF4pawntard]

Life is absolutely amazing.

[@dennisa7587]

When the jar heats the air pressurized out and when it cooled the vacuum was not strong to suck air back in

[@GlassOasis]

Could be the case... The seal is pretty tight, but temp change and build up of gases in the early months could have caused some leakage for sure.

[@Rorther]

After you opened it, you reclosed it? Would biodiversity go up or down in this case? I'd say lower because the drastic change on CO2 and Oxigen.

[@GlassOasis]

Maybe a bit down... But it's been closed for a year already, so no considerably. The snails might be the first to go if there is some big swing in the equilibrium inside. Otherwise, I'd hope most of this will survive for years to come. Most of decrease in biodiversity happened in the first few months, probably.

[@thedavephan]

The pressure situation... thepressur inside increased and the air actually seeped out over time. But then it couldn't replace that air = negpressure

[@WhatsCookinToday]

I was so interested with what was in the jar that I forgot you planned on opening it.

[@GlassOasis]

🤣 Go watch some of my other videos then!

[@john-brady]

Amazing. I have to make one

[@henrytang2203]

1 year without a water change. This does much better than most people do with goldfish and weekly water changes.

[@roseogrady8785]

Fascinating. Thank You.

[@williswilson8147]

1 mol of gas at STP= 22.4 liters. Atomic weight of O2 is smaller than C02. You have plants that consume CO2 and not a whole lot that consumes O2 in there. You created a higher molar ratio of O2 in the environment therefor lower total pressure inside the container. Simplest and most obviously correct answer in my opinion.

[@wheelerking8380]

if you drink it all….I subscribe Choose wisely

[@BetaMayra]

Maybe the low pressure is because you opened the jar in a colder environment than when you sealed it?

[@yahbless1659]

These types of jars and its seal allow pressure to escape but not air to come into the the jar.. I learnt this through food fermentation.

[@kanzegy4637]

its just a theory but: maybe the negative presure is because some of the oxygen and CO2 and other gases un the tank, is now underground or being a part of the animals and plants, wich means, in a solid state those gases ocupie less space, causing some kind of vacum and maybe if more time passes, eventually all the gases run out and then everithing there could die there... of it could just sutain forever

[@insanitysymphony9175]

return of the king

[@TinusBruins]

The low pressure is due t the type of jar, the lit/seal on these are designed to let air out easier then air in. Before refrigerators these we're used a lot to preserve food.

[@GlassOasis]

I suspect that is the case

[@peetsnort]

The perfect example of nature balancing

[@aaronjames7266]

Exactly. A closed system will always balance based on available resources, which is exactly why the lie that the earth is overpopulated is just that - a lie. We don't need to ever worry about that in a closed system.

[@gerryk101]

You had an air pocket big enough to sustain the environment at scale and because of the plant. I think if it was filled to the top the outcome would have been even more microscopic.

[@rustynail7609]

"negative pressure"? Or vacuum I wager was caused by a temperature inversion after being removed from the light source it was "under".

[@python471]

Great video! Love these jarrariums. Is there a list of music for the more ambient stuff around the 3:00 & 6:00 marks? Great choices!

[@GlassOasis]

That's "Dreamer" by DivKid at around 3:00 and "Eureka" by Huma-Huma at around 6:00. I agree, they are great songs!

[@bigrich6750]

I would bet the seal happened for the same reason a mason jar seals when you’re canning food. You put the river water in warm and as it sat inside the A/C and cooled the pressure lowered inside the jar based on Boyles Law, creating the seal. That would be my first guess. There may have been some pressure reduction from the displacement of oxygen as it was used up, but that would mean another gas, methane or CO2 replaced it and I think gas is gas in terms of pressure. In other words, as long as the column of gas stays the same, the pressure would not change unless the temperature changed, and that’s what I’m going with.

[@angelawildman122]

This is why raw water isn’t safe to drink unless it’s from a clean well or certain clean springs.

[@GlassOasis]

It's not what you can see in this video that'll hurt you (unless there's some parasitic worms) it's the things too small to see.

[@luckylillyHasLuck]

How do u only have 1k subs? Don’t worry, by the end of this year you’re bound to have 100k subs! And you don’t have to but remember me when you’re famous >:)

[@GlassOasis]

Thank you so much for the encouragement and kind words!

[@BlueShadow777]

Well, if all the videos have similarly intruding, annoying music… then I’m not surprised 🤷🏻‍♂️

[@tedmihalca]

the suspense i felt while he was opening the jar was that of the Tsar bomba about to go off

[@russellsheridan3957]

I think low pressure formed from condensation outside the jar.

[@pleasedontwatchthese9593]

The low pressures could have been where/when/temperature you closed it it. Kind of like how car tires get deflated when it gets cold.

[@susanh3342]

Could you show us how to make a jar like that??

[@GlassOasis]

Yep, I'm sure I'll talk more about it in future videos.

[@bettauniverse777]

Hi I am the 298Sub😊 keep up the good work bro soon you will have 1m sub

[@GlassOasis]

Thanks so much!

[@Lazarus-aap]

Temperature difference between the day you closed it and the day you opened it

[@ghostbirdlary]

probably the low pressure was probably from air leaking out when it was higher pressure from heat and not being able to leak in when it was lower pressure because the atmosphere pushed on the seal more

[@joeyshumate2564]

water temperature difference between sealing and opening would cause negative pressure

[@chancefugitt4329]

Mine turned two years old, a couple weeks ago.

[@assistantto007]

I think you had a slightly lower pressure inside the container due to evaporation of the river water.

[@Lardzor]

I think the jar was low pressure because oxygen in the air reacted with material in the jar. Roughly 20% of normal air is oxygen, if all the oxygen in the jar reacted with other material in the jar to form oxide compounds, that would reduce the gas volume in the jar by 20%.

[@coinisinorbit]

Can you so a jar with a splash of sweetened tea? Wanna see what grows inside

[@chemicalvamp]

I think the pressure lowered because the plants in the jar had absorbed probably all of the carbon dioxide and nitrogen in the air, and water. Using it to build with. Anything the plant(s) had shed would be gobbled up by the decomposition feeders like worms and snails. (Dissolved) Gasses into a solid kind of thing, I don't think that extreme could be explained by barometric pressure alone, And I feel like if there was not a bottleneck of dissolved mineral gasses, it would have grown to an extreme "Off a leash".

[@enbyNTT]

How do you have less than 1k subs, here before you get famous 🎉

[@GlassOasis]

Thanks for the encouragement!

[@andrewvogan3425]

I'm guessing that you sealed the jar at a higher elevation than where you opened it. You would have sealed in the lower air pressure from the higher elevation.

[@hussaineh89]

The smell of earth broil cuz of temperature buildup under constant indoor lighting and that vapor having a lower density. You must try another jar kept outdoors for 365 days, and if you are in a snowing place - even better

[@IceSpicenberg]

Keep the work up bro I’m here from Reddit

[@GlassOasis]

Glad to have you! I love the closed ecosystem community over there!

[@mariaconcepcionrodriguezhe2850]

🤢🤮not a redditurd!

[@TK_mushy]

I think the pressure is bc some water evaporated & air wasn’t able to get into the jar to take its place

[@b1gm0n3y]

My guess is maybe some water evaporated and made it suction

[@thehairywoodsman5644]

I subscribed , cool video, my only request, take a sip of the water !

[@SkylakekungCh1000]

that too loud music so good i even laugh at it lol

[@SkylakekungCh1000]

*too loud music joke

[@abbosimmo]

The vacuumed seal has got to be due to the various gas reactions that have happened over the year, not a scientist so can’t say which gases would cause a vacuum rather than a pressure build up that causes the lid to pop off, perhaps loss of certain gases only extracted through the “standard” air around the jar caused an implosive rather than explosive effect

[@LuMaxQFPV]

Audio bursts and variabilities make it hard to listen to. ALC or processing can help. I had to change channels.. the booming loud, then soft, etc. was hard.

[@GlassOasis]

Yep, I went back and listened to this and, for how popular the video has been, I really wish I spent more time optimizing the audio

[@GlassOasis]

My more recent videos hopefully have audio that is easier to listen to.

[@osamabentrappin4102]

Bro you put a world inside a jar for a year it made its own eco system damn near its own forecast an everything I would assume after that long the simple answer is the life forms have created a low pressure resulting in the ecosystem wanting to not be opened

[@DonkeyButter1]

love this channel

[@kj_H65f]

If the jar was perfectly sealed and gas didnt escape, i can only assume that N2 was somehow made bioavailable. Otherwise Id have to assume gas somehow escaped... i dont know but its fascinating.

[@tylermiller3228]

The low pressure is probably from the life sucking the oxygen out of the water and the air trapped in the jar

[@rockyreyes9320]

The only reason they would be a high-pressure carbon structures we're being decomposed to produce CO2 gas which is more voluminous. So considering that there is no excess of oxygen for the oxidation of carbon to occur then no gas would be able to build up. Said production of carbon-based structures and compounds are why the pressure in the jar was less than atmospheric pressure.

[@bobthebuilder69429]

I think there was a negative pressure because there is less oxygen inside than outside. And whatever gasses did form exerted less pressure than the atmosphere outside.

[@consentofthegoverned5145]

The earth goes year after year, sealed in it's own atmosphere without any fresh air from the universe.

[@GlassOasis]

Yep. Pretty cool to think about.