Microscopy: Fluorescent Probes (Timothy Mitchison)

Learn more: www.ibiology.org/talks/fluore...
This lecture describes the chemical properties of organic dyes and quantum dots (Q dots) that make them fluoresce and how their fluorescence wavelength is tuned. It also describes how dyes can be attached to proteins, photobleaching, and other dyes that bind DNA (Hoeschst) or organelles (mitotracker, lysotracker).

[@sciencestudent88]

Amazing video, totally need this for flow cytometry!

[@stewjw]

Nice lecture. For the hard question. I assume at low pH the ester at the bottom will become protonated. If you move a pair of electrons from one of the sugar oxygens attached to the tricyclic rings at the top of the molecule. You can move them around (curly arrows) to release the carboxy group at the bottom to give you the extended conjugated structure which will now fluoresce. The freed up carboxyl group can then be used for further conjugation reactions. Very neat.

[@creativewriter3672]

Thanks a lot sir... Wonderful video

[@eniemeuful]

Wow! Real life Sheldon!

[@fluorescentchemist7484]

Which probes can be used for targeting of lysosomes

[@sollinw]

GREAT!!!

[@pecatum666]

What software can be used to create a video like this?

[@lbochtler]

pecatum666 adobe after effects with a screencapture software and a greenscreen as a background.

[@pranavgadhave7060]

Are Fluorescence labels and Fluorescence probes are same ??

[@WulinTeo]

This is one of the lectures I found it very informative. Especially the HOMO and LUMO relation. Most lectures they just skip this fundamental principle. By the way, why not call HOMO highest occupied ELECTRON orbital instead of molecular? after all, it is the electron causing such phenomena. Thanks!

[@mrmadmaxalot]

+Wulin Teo This is an old comment, but since no one responded, the reason is because the term comes from molecular orbital theory which makes distinction between atomic orbitals and molecular orbitals. Since these are molecules the behaviour of the molecular orbital is what they are working with.

[@WulinTeo]

+bv Thanks!