Microscopy: Super-Resolution: Structured Illumination Microscopy (SIM) (David Agard)

[@nicolasb3362]

I have a presentation to do on SIM tomorrow and I found this video to be a very good summary/revision material. Thanks!

[@yoselinmerarilopez8631]

Yo también y hablo español no entendí mucho :(

[@jmckendry84]

Really excellently clear explanation of a complex topic, even to a relative layman like myself. Thank you!

[@scicreep8979]

I would appreciate if you add subtitles. It facilitates understanding for those like me who do not dominate the English language. Great job! Thanks. :)

[@ashwinwagadarikar2529]

Fantastic explanation of structured illumination. Thanks!

[@lollsazz]

Great explanation of something that is very hard to grasp :)

[@NehadHirmiz]

Excellent Lecture. Thank you very much

[@mksboysal]

Sir, do you have any idea why: Dark field microscopy is not used in hospitals and medical schools?

[@owlredshift]

Did u just assume his gender 9 years ago

[@rajkaur17]

Excellent video

[@k4mb]

Hello

[@MrHuddo]

+Hardeep Singh LOL^

[@zhouzhouzhou672]

this is very impressive

[@marioariyanto8382]

exactly what you need when you are new in this field

[@kachorrosoft]

Amazing! Thanks a lot!

[@jameselger572]

Just use 3 Tunable monochromators. Then spectral analysis results to fine tune that to the bulk absorption bands of a biological sample. Doesn't matter what we see, or not.

[@jameselger572]

Upgrade, three cameras on a track, the image infinity corrected, sent through a prism and the absorption wavelength sent is isolated as a image some distance on that frequency of light. By having the ccds move on tracks just places a distance between the prism required to isolate the wavelength specific image. We only illuminate with absorption bands because, they cast shadows.

[@jameselger572]

Facts on the net are public info. I know its a release by typing it in public.

[@jameselger572]

If you can't see it, use a spectrometer, a white light source and figure out which colors to use, and move the ccds to.

[@jameselger572]

I would think you would need a image amplifier to move the highest resolution of light because biological samples do not like being flooded with UV, which means using allot less light for a high resolution image, you always want the sample to survive.

[@jameselger572]

I don't know of any UV spectrum spectrometers. Infrared, was remapping by diffusion, wavelength shifting, autoflorescence? absorption, and refraction. White light is mapped but, starting with say a monochromatic source of light, you look at all the ways the wavelength you sent, changed by absorption, diffusion, frequency shift and you'll see the monochromatic source, spreads.

[@atlantiswasinafrica]

This has been further improved upon using MSIM and Instant SIM

[@HML2004]

Wow!

[@rugbykid1104]

This comment section is unreal.

[@vancelemuel5352]

ayos pala eh

[@chon8347]

oo nga eh

[@vancelemuel5352]

lol

[@neytiritetskahamoatite7688]

Can i see THE WORMS FROM OUR MASKS WITH MICRO HOME ??? Cause WE HAVE WORMS IN AUR MASKS, ALL OVER THE WORLD. NO JOKE !!!