Increase NAD With Fish Oil (And Other PUFA-Containing) Fatty Acids? Hypothesis.

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Papers referenced in the video:
Effects of Peroxisome-Proliferators on the Trp-Nad Pathway
pubmed.ncbi.nl...
Hypolipidemic drugs, polyunsaturated fatty acids, and eicosanoids are ligands for peroxisome proliferator-activated receptors alpha and delta
pubmed.ncbi.nl...
PPAR-a as a Key Nutritional and Environmental Sensor for Metabolic Adaptation pubmed.ncbi.nl...
Metagenomic and metabolomic remodeling in nonagenarians and centenarians and its association with genetic and socioeconomic factors
pubmed.ncbi.nl...
Positive regulation of the peroxisomal/3-oxidation pathway by fatty acids through activation of peroxisome proliferator-activated receptors (PPAR)
pubmed.ncbi.nl...

[@nwobob]

c16 saturated fat is palmitic. c18 is stearic. You are correct in noting cocoa is a rich source of stearic acid. As is tallow.

[@conqueragingordietrying123]

You're right, thanks @nwobob, and apologies for my mistake!

[@kindedge]

Well, to clarify, the stearic acid is not in cocoa. It is in the cacao bean which contains the 50% fat that is cocoa butter, the source of stearic acid.

[@conqueragingordietrying123]

@@kindedge I eat whole, ground cacao beans, so there is stearic acid from that in my diet...

[@bgsharma875]

In any case, Acetyl-CoA -->Malyonl-CoA via DNL goes through Palmitate. Cytosolic fatty acid synthase (FAS) complex catalyzes the reaction of acetyl-CoA with 7 malonyl-CoA and 14 NADHP + 14 H+ to form a molecule of palmitate and 7 CO2, 14 NADP+, 8 CoASH, and 6 H2O.

[@framexdd]

So you wanna increase DHA so that you can increase PPAR activation so that you can increase gene expression of QAPRT so that you can increase the conversion of tryptophan into NAD and so that the increase of NAD into the tissues is reflected into the blood so you can check with jinfinity. I have low hopes that it will work BUT if it does you get 2 birds with 1 stone (+NAD & +FGF21). Loving the videos lately, full of new ideas and possible mechanisms to improve X organ/biomarker.

[@gstlynx]

Thanks Doc, I really appreciate your rigorous detailed analysis and explanations. I believe your students are lucky to have you.

[@conqueragingordietrying123]

Thanks @gstlynx. Maybe sooner than later, there will be more students, and outside of a university-perhaps on this YT channel!

[@markemdee7311]

Thanks for your effort and sharing your results. I take two fish oil capsules each day, and about 50 different supplements during the week. Also 1/4 cup of chia seed each day.

[@conqueragingordietrying123]

Thanks for your support, @markemdee7311! I added 2g of fish oil this week, so we'll see if it can increase NAD within the next month.

[@FindsWay]

Consuming PUFAs for PPARa activation should lead to a decrease in NAD+ and an increase in NADH. PUFA overdose in diet leads to a torpid metabolism and lipogenesis. For further details on how, please watch the videos of “fire in a bottle”. Brad is doing an excellent job over there.

[@conqueragingordietrying123]

We'll see, I'm testing NAD levels in about 3 weeks.

[@rogerbritus9378]

couldn't locate "fire in a bottle" - is it a YT channel?

[@conqueragingordietrying123]

@@rogerbritus9378 Yep, www.youtube.com/@fireinabottle3410

[@umbanat6970]

@@rogerbritus9378 It also a blog, first. You easily google it.

[@Chickenbbbk]

Instant sub ❤!

[@berdi4berdi4]

Looking forward for that study. Thanks for sharing!

[@bgsharma875]

Good video but I would be a little careful in adding more PUFA- The H+ on double bonds are easily removed ( more the unsaturation more is the risk) resulting in free electron that can cause superoxide- If antioxidants can not handle, NADH/NAD+ ratio can increase due to reverse flow and increases inflammation.. Also PUFA remains in system for long time and can cause lipid peroxidation besides HNE from superoxide/electrons/inflammation etc .

[@conqueragingordietrying123]

Thanks @bgsharma875. I understand that idea, but if it's true, that would mean O3 and flaxseed (whole, not oil) increase inflammation, and afaik, there isn't a published consensus to support that hypothesis. Alternatively, I'll do a metabolomic analysis in conjunction with the next NAD test, so if there's an increase for oxidative stress, we'll see it in the metabolite ratios.

[@bgsharma875]

Good. Perhaps Flaxseed etc. quantity is low enough to cause issues. There may be a threshold when antioxidants can not handle the reverse flow. No idea on that threshold in addition to everyone has different amounts of GSH/Catalase etc. in addition to O2/superoxide/peroxide/Hydroxyl etc.

[@conqueragingordietrying123]

Definitely,@@bgsharma875

[@diamond_s]

@@conqueragingordietrying123 omega 6 pufas seem to be negative in excess, but omega 3s seem like the signaling they do is good enough to counter any potential downsides. Also the body knows to ramp up antioxidant mechanisms if it finds it needs them. And last I heard at least for some unsaturated fats the body has the ability to saturate excess and keep careful control of membrane fatty acid composition even if extreme levels of unsaturated fats are given in diet. Membrane composition is said to explain away the exceptions to rate of living theory of aging, the animals with high lifespan and high metabolism simply have more peroxidation resistant membranes. It has been observed that the longer lived a species the more resistant the membranes, and this is even more so in species that live for centuries. Calorie Restriction is said to increase membrane peroxidation resistance, I suspect given gene expression changes have been similar in some CR mimetic studies CR mimetics might also increase membrane peroxidation resistance. Another interesting compound has been Astaxanthin, the intervention testing study used the inferior synthetic form of Astaxanthin and despite that obtained some amount of life extension in rodents. I suspect natural Astaxanthin as one of the best membrane protectors will do even better if it ever gets tested.

[@sarpsomer]

Great Presentation. Zero points missed.

[@MixtrisUFOimages]

Fantastic stuff thanks for the update Very interesting Looking forward to this one as I consume all of those fatty acids daily

[@conqueragingordietrying123]

Same, but ~9g of O3/d, from both fish and ALA might not be enough, at least for. The plan is to increase that to 12-15g/d, and then test NAD levels.

[@MixtrisUFOimages]

@@conqueragingordietrying123 that shouldn't be too difficult to increase Fingers crossed 🤞

[@pettersbrevlada]

Thanks so much for your videos! Have you discussed the applicability of the blood NAD-levels to other tissues? One common reason to not supplement with NMN/NR is that it is uncertain how much the tissue levels increase vs the blood levels.

[@conqueragingordietrying123]

Thanks @pettersbrevlada. Even with NMN or NR, it's a challenge to extrapolate blood levels of NAD to what's inside tissues. I think one way to get around that is by using metabolomics, where markers like acylcarnitines and TCA cycle metabolites can inform about mitochondrial function, which would be expected to be better with relatively higher NAD levels.

[@rapamune]

PPARa seemed to play a dramatic role in pre-adipocyte differentiation (maturation), however, the PPARa (transcription factor) binding sites underwent drastic chromatin remodeling during this process. Most of the functions of PPARa were indicated to be superceded by PPARb. Should this pathway be based on such a model, it may be relevant to consider

[@g.s.5868]

It was patented in 1958 by Imperial Chemical Industries and approved for medical use in 1963. *Clofibrate was discontinued in 2002 due to adverse effects*

[@conqueragingordietrying123]

Yes, thanks

[@ddutton4716]

Fascinating, informative, and another approach. How can DHA give an activation of over 100%? Thanks.

[@phil.1]

Niacin (nicotinic acid) is S+ tier. Taking 2-6g daily whenever I scoop it into my citrus drinks each day haha. I love the flush, it’s like a sauna and feel extremely relaxing afterwards. Now my ultimate question is if I put Niacin powder mixed in my skincare lotions, would it have the same effects as Niacinamide (nicotinamide) topically? Like Niacinamide is anti-aging youthfulness for the skin and I wonder if the skin can convert the topical niacin and have similar effects.

[@kevin1405]

From my understanding, niacinamide is better for skin, but nicotinic acid is better to ingest, and ingesting it will also benefit your skin. However, I read something about niacinamide on skin potentially causing hair near it to go gray. I think it had to do with SIRT1, but I'm not sure if that was just speculation or not.

[@phil.1]

@@kevin1405 so after googling, i know why now: nicotinic acid (niacin) will work exactly the same as nicotinamide (niacinamide) on the skin cells... HOWEVER, nicotinamide penetrates the skin barrier much more easily and more significantly on orders of magnitude to reach the skin cells to work it's NAD+ magic! hence why skin formulation will use nicotinamide. time for me to buy both bulk niacin powder (15usd) and niacinamide powder (15usd) each haha. one for oral ingestion, and one to mix in my skin serums and pH balancing it for better skin penetration of active ingredients

[@lookfeelbehealthy6320]

This is awesome! Do we know how many mg of DHA we'd need to consume for this effect, and even EPA for that matter? I'm currently taking a fish oil supplement with 900mg of DHA and 1200mg of EPA.

[@conqueragingordietrying123]

I've added about that much EPA+DHA to my current approach, and will test NAD levels in a few weeks. Stay tuned...

[@lookfeelbehealthy6320]

@@conqueragingordietrying123 Great thanks!

[@notjasonhowarth]

Love your work 🙏

[@conqueragingordietrying123]

Thanks @TheJasonHowarth!

[@andrew33933]

just please test your inflamation factors in sept. and how much PUFAS you are going to consume. thanks

[@conqueragingordietrying123]

@@andrew33933 Note that hsCRP has been < 0.3 mg/L for 11 consecutive tests, and I doubt that more O3 will increase inflammation, but I'll test CRP again, for sure.

[@andrew33933]

I read that The PPAR agonists are known to regulate expression of the gene for cyclooxygenase-2, which in turn plays an important role in lipid metabolism, mediation of inflammatory pathways and tumor promotion. so be careful,.

[@conqueragingordietrying123]

If there are studies with an increased cancer risk with fish oil or flaxseed intake, please post them...

[@jamesgilmore8192]

@@conqueragingordietrying123 Mike here is the latest I'm aware of: Omega-3, omega-6 and total dietary polyunsaturated fat on cancer incidence: systematic review and meta-analysis of randomised trials, Hanson et al British Journal of Cancer volume (2020). This might be a situation where cycling is better than constant dosing. For your experiment if the goal is to get the O3 through to the nucleus, I assume you'll need to saturate many PUFA pathways to transport appreciably into the nucleus and so you might be better off with a higher dose than you planned, then cycle off, and cycle on at some point later.

[@andrew33933]

the link is getting deleted to sciencedirect

[@andrew33933]

Peroxisome proliferator-activated receptors (PPARs) by Shayne Gad 2023 in Reference module in Biomedical sciences. and that was an update from an article from 2014 also interesting (PPAR agonist troglitazone was removed from the marked)

[@jamesgilmore8192]

@@andrew33933 yes youtube deletes most links, Mike can post links though, but we need to write them out as text 😀

[@monnoo8221]

linoleic, alpha- linolenic : also in fenugreek yet, without the salvage pathway working well, which is NAMPT expressed and working well, the NAPRT improvement will not raise levels very much. Salvage pathway is the dominat pathway by far, the others only for backup and replacing losses. some say, rutin helps, which is in buckwheat, apricots, asparagus, cherries, grapes, grapefruit, beside troxerutin. The latters manifold actions may well be all linked to increased NAD levels

[@conqueragingordietrying123]

I keep hearing that salvage > de novo for NAD levels, and maybe that's true via supplementation, but endogenously, I've yet to see a head-to-head matchup published with normal intakes of tryptophan and niacin (~1g/d, 15 mg/d, respectively).

[@monnoo8221]

@@conqueragingordietrying123 if asparagus or buckwheat do not have bad influence on your bio markers... You can just test in parallel (?) , without drastic precursor supplementation

[@justsaying7065]

@@conqueragingordietrying123 That research has been done. General guidelines indicate that 60mg of tryptophan is equal to 1mg of niacin. So 1g of tryptophan is equal to 16.67mg of niacin. The 60 conversion factor was determined in 1956 in a human study of men. There have been other studies since then that report slightly different conversion factors, but 60 is still the number reported in general guidelines. For example, there is a paper titled "Conversion ratio of tryptophan to niacin in Japanese women fed a purified diet conforming to the Japanese Dietary Reference Intakes." In this experiment, they calculated that 67mg of tryptophan was equal to 1mg of niacin, pretty close to the 60 quoted above.

[@bogrunberger]

Thanks for this, it's helped me understand a lot. One thing about boosting NAD levels: AFAIK it's only blood NAD that goes up, and NAD in muscle and brain remains the same. So does it really matter that we boost NAD?

[@larsnystrom6698]

In this paper they used Nicotinic Acid to boost NAD. They measured NAD both in muscle and blood. They concluded that blood values could be used as a diagnostics for NAD in muscles, because these values are related. Niacin Cures Systemic NAD+ Deficiency and Improves Muscle Performance in Adult-Onset Mitochondrial Myopathy Eija Pirinen, et al

[@conqueragingordietrying123]

Yep, I agree with your premise, but alternatively, what if there's a tissue increase for NAD-would that be reflected in a higher blood NAD concentration? One way to assess that is with metabolomics, which I'm doing on the same day as most of the NAD tests.

[@justsaying7065]

How many grams of SFA are you switching to PUFA? How many grams of PUFA were you eating before the switch?

[@conqueragingordietrying123]

Hey @justsaying7065, the plan is to double my current intake, so about 4g/d of EPA + DHA.

[@KenOtwell]

Great stuff - thanks from this 67-year old (going on 120).

[@conqueragingordietrying123]

Thanks @KenOtwell! Even better will be if it works...

[@Battery-kf4vu]

The thing is, it is not because NAD increases in the blood that it increases in the tissues. Do you think that by doing it this way NAD will go up in the tissues?

[@conqueragingordietrying123]

Based on the rat study in the video, yes, but your point is a good one-there may not be a blood NAD increase but increased liver levels, for example.

[@petermilian4455]

Great insights! I do 2 tbsp ground flax in my daily breakfast of black lentil curry. Also add 1 tbsp of chia to my daily afternoon berry smoothie. And 1000mg EPA fish oil daily - 500mg AM & PM. I'm whole food plant based and was previously taking an algae based EPA / DHA blend but recently switched. Bryan Johnson is mostly vegan and his team still thinks it's worthwhile for him to make an exception to take 1000mg EPA rTG daily (only currently available as fish oil) so I switched to that.

[@AstonAcademia]

Curry for breakfast? How'd you make it if you can share.

[@jackbuaer3828]

I take the Algae form without rtg. 1 tablespoon of the Algae oil I buy is 1200 DHA / 600 EPA. I take two tablespoon daily, so it's a pretty high dose.

[@NoCredentialsNeeded]

​@@jackbuaer38284 g is healthy

[@AstonAcademia]

@@user-lw3qt6jd6y Thanks

[@mime454]

Worth getting a Costco membership to buy the Sports Research Fish Oil there. IFOS tested for low contamination and heavy metals, 1g EPA+DHA for $.18(in the 150 pack). Less than half the price of amazon. I take 6 per day and don't even get fish burps.

[@conqueragingordietrying123]

I'm not sure which is best in terms, but that's for the recommendation.

[@jamesgilmore8192]

​@@conqueragingordietrying123 Well at least in 2015, most weren't great quality: Fish oil supplements in New Zealand are highly oxidised and do not meet label content of n-3 PUFA Albert et al Scientific reports 2015. Maybe things have improved, but finding a "pure" EPA or DHA is probably a tough ask. I've heard EPA with Vit E might be better quality, but I've never seen any testing on the combination.

[@مازلتعلىقيدالحياةلابدمنمخرج]

What is your say on saturated vs unsaturated fatty acids?

[@jonese007]

I wonder if you will see an NAD sparing effect when taking fish oil? EPA/DHA rebalancing gram positive/negative bacteria levels in the gut + reduced leaky gut + reduced LPS in blood. This should reduce CD38 expression + reduce inflammation + spare NAD needed for CD38. Also, improved omega 3 index should improve cell mambrane fiunction over time. Your thoughts?

[@conqueragingordietrying123]

i sent that sample for analysis last Monday, results should bein this week!

[@jackbuaer3828]

My Algae Oil has a 2 to 1 ratio of DHA to EPA. 1 tablespoon has 1200mg of DHA. Perhaps that's a good source and it has no fishy smell

[@rossinou]

Very expensive though, no?

[@conqueragingordietrying123]

Definitely-which brand? Also, any fish burps afterward?

[@jackbuaer3828]

No fish burps. Brand is NutraVege by Nature's Way, Omega-3 Plant, Extra Strength, Cranberry Orange, 6.8 fl oz. A bottle is about $35. As far as I know, this is the most economical algae oil sold as a supplement and perhaps the only one that comes in liquid form. Everything else seems to come in capsules and has carageenan. They lab test for heavy metals / oxidation / DHA/ EPA levels. You can look at the lab results by lot number. The testing looked very comprehensive to me from a lay person's perspective, but I only looked at the testing results once

[@g.s.5868]

@@jackbuaer3828humans do not have a good plant epa conversion...

[@jerryh2897]

Why not consider polyphenols and monounsaturated fatty acids in extra virgin olive oil? Do you have relevant data? Thanks❤

[@conqueragingordietrying123]

I can go over MUFAs in another video, but they're weak PPARa activators relative to PUFAs. Other than resveratrol, which polyphenols would you expect to increase NAD?

[@p_peja]

Great work! Is the one month of dietary change enough to produce significant differences in NAD measured levels?

[@conqueragingordietrying123]

Yes, definitely. I’ve seen changes for NAD levels in as short as 1 week of supplementation.

[@sooooooooDark]

after googling around a bit it seems that there is some connection between ppar-alpha and hdl (from what i understand it is that ppar-alpha increases hdl, not the other way around...even tho chatgpt tried to suggest me that 🙄) i checked ur website and saw (idk if that entry in the hdl thingy is uptodate) that ur hdl level wasnt optimal (according to ur own standards) therefore ur approach to increase ppar-alpha might also lead to an increase in hdl, which would probably also welcome 😊maybe keep an eye on that association next test as well

[@conqueragingordietrying123]

HDL is currently 54, 2 tests in a row. For men, 50-60 is associated with lowest ACM risk, so there's been progress!

[@sooooooooDark]

@@conqueragingordietrying123 ah it said 40 on ur website i think 😅 hard to keep all ur levels in back of mind, maybe put in description of each new vid so u dont have to waste time writing stuff over and over

[@conqueragingordietrying123]

@@sooooooooDark The website may be old news, check out my biological age playlists on YT

[@sooooooooDark]

@@conqueragingordietrying123 would be cool to have something like a discord server as a hub for resources/sharing studies and stuff this yt comment thing is kinda slow and awkward xD

[@conqueragingordietrying123]

@@sooooooooDark I have a Patreon acct...

[@Pyriold]

Very interested. I am taking 5 grams of fish oil each day for other reasons (i totally got rid of my dry eyes that way, got that from a published study and it worked for me). So please post your results ASAP :).

[@conqueragingordietrying123]

Will do! I have a dry eye, too, so it will be an interesting experiment for another reason. We're probably 1 month away from the FO-NAD experiment, as I want to take it for at least 2 weeks.

[@Pyriold]

@@conqueragingordietrying123 Takes about a month of high dose fish oil to cure the dry eye (if it works for you). Worked for me and a friend of me, so chances should be good. I think in the study it was above 70% of people.

[@conqueragingordietrying123]

Interesting, thanks for that @@jameslovelace4809

[@bearbaler1456]

Hi there. Have you done a video on Apigenin from Parsley. (Blocking CD38) Thanks for the great content.

[@monnoo8221]

Yes he did..

[@conqueragingordietrying123]

Yep, it’s on the channel

[@bearbaler1456]

Thanks very much@@conqueragingordietrying123

[@luckssj]

I had my NAD tested and I take NMN and my NAD dropped and my CD38 dropped

[@conqueragingordietrying123]

Sorry to hear that, why do you think NAD dropped, despite taking NMN?

[@musaire]

Probably this info is presented in earlier videos, but does Jinfiniti test the NAD+ levels in blood cells or does it rather test it in the blood plasma? Or both options available? I have heard the cell levels could be misleading (because the cells in body can have different NAPRT and NADS levels expressed, and so even if certain NAD+ levels increased in blood cells, let's say, by nicotinic acid, we don't know that NAD+ is shared to other organs and whether the enzyme work there, plasma levels could be more interesting, demonstrating NAD+ being shared around, maybe).

[@conqueragingordietrying123]

Hey @0626love , blood cells, and yep, fair point about what's going on in tissues. If blood cell NAD levels are low, I'd bet that tissue levels are low, too. I'm currently sending blood for NAD and metabolomic analysis on the same day of testing, to get more potential insight about tissue NAD levels.

[@musaire]

@@conqueragingordietrying123 Nice! I'm taking nicotinic acid myself, 1000mg doses, but never measured NAD+. so I was really impressed by the effect of raised niacin dose in your case, just hoping the blood reading is not false positive so-to-speak. Definitely NAPRT and NADS are highly expressed in liver - I'm hoping the liver shares the overflow of NMN or some NAD+ but not that much in niacinamide form (because niacinamide molecule competes with NAD+ itself on the NAD-consumer end, and so shuts the NAD-consumers down - neutralization of excess niacinamide important - so also the cofactors for NNMT enzyme and aldehyde oxidase - luckily sweating also removes some niacinamide (without the need for methylation), partly why sports and especially sauna is good for NAD+ flow).

[@wjm52358]

You might look into taking Flaxseed/linseed oil to get larger amounts of ALAs.

[@conqueragingordietrying123]

Whole foods over processed (i.e. flaxseed oil) wherever possible, but I can see a path for fish oil in addition to sardines. If I eat more sardines, I'm at the border of not being satiated by having to take out other stuff. It's a relatively easy fix with only ~50 calories/d from fish oil, though.

[@user-qr2pr2rx2f]

Watch your SHBG with flax seeds!

[@conqueragingordietrying123]

Thanks for that, did your increase with higher flax? I'm thinking about not increasing flax, but solely fish oil.

[@user-qr2pr2rx2f]

@@conqueragingordietrying123 It's just something that I learn lately.

[@jpintero6330]

OMG, please tell me more!

[@KoiRun50]

I had a great 10k treadmill run once after consuming a can of cod liver pate/oil. Do you think this can be due to a bump in NAD? Could it work that fast?

[@conqueragingordietrying123]

It would be great if it could work that fast...

[@diamond_s]

iirc Dr. Bill Andrews an antiaging researcher recommended like 1g of DHA and 1g of EPA per day. To reach that even fish oil pills need like 6, so what I do is I take two triple strength fish oil pills which are equivalent to six regular pills. I also eat wild caught salmon.

[@conqueragingordietrying123]

Beyond general recommendations, a primary focus of the channel is to determine how much FO or other supplements may be sufficient with regular, objective biomarker testing.

[@diamond_s]

@@conqueragingordietrying123 hope its found doses can be lower, cause that'd be far more economical.

[@luckssj]

Have you looked at Nitric Oxide, NMN, D3, and Fisetin?

[@conqueragingordietrying123]

Yes on NMN, see kzbin.info/www/bejne/bZCmimqCp6uDrbc Why would you expect the others to increase NAD?

[@ScottSummerill]

Maybe take flax seed oil for your ALA. Think you'd need a heck of a lot of flax seed to make any difference.

[@conqueragingordietrying123]

For the next NAD test, after seeing my recent blood test results (Monday, 8/21), I'm thinking about shifting my flax intake back to where it was, but increasing EPA+DHA instead.

[@andrewrivera4029]

My wife and I just incorporated flax seed into our diet for the possible cardio protective properties and we already take fish oil will work to get chia into our diets. I’d rather be eating these thing than a Big Mac! Lol Thanks for the great work!

[@squarz]

There is no risk of excess of vitamin A and heavy metals from fish oil? Better try only with seeds?

[@conqueragingordietrying123]

I started with an increase for flaxseeds, but I may be maxed out in terms of their potential health benefits. In support of that, despite their increase, one of my worst blood test results will be posted in Sunday's video. I don't have NAD data for that test yet, though. With that in mind, I tried going higher for sardines, but I'm maxed out there, too, as more starts to take away from overall dietary satiety. The amount of Vitamin A in 30 calories of fish oil is trivial...

[@squarz]

@@conqueragingordietrying123 yeah. My father ended in dialysis about ten years ago. Because of unnecessary drugs that ruined his kidneys. So I tend to take the less amount possible of supplements that most of the time lacks quality control. I only take b12 and some D in winter that should be enough. I've taken fish oil in the past. It helped in a moment where I was surely not eating well but due also to the state of the oceans pollution and the amount of waste that is fish oil production I will never took it again. I'm hoping that the amount of chia and walnuts can be enough (I can't see myself grounding flaxseeds for the entire life and I think that pregdounded flax can go rancid easily)

[@edwhite2255]

Michael, do you grind your flaxseed?

[@user-ur2bx2yx4r]

It will be no digested if you don't grind it

[@conqueragingordietrying123]

Hey Ed, yep, I but it ground-it tasted better (to me) like that. Also golden variety.

[@DIYDSP]

I recommend grinding and storing in fridge sealed in nitrogen if you can swing it. Pre-ground may get rancid from temperature in transit and shelftime is unknowable. It may lose helpful properties, even before you can taste the difference.

[@conqueragingordietrying123]

@@DIYDSP Anecdotally, I've never had an issue with ground flaxseed being rancid, or any other nuts.

[@juliahello6673]

Try taking a spoonful and just chewing it. It tastes good.

[@kamanashisroy]

Very exciting. Some drug company will do dubious study to promote their products while there are healthy food alternatives are there.

[@conqueragingordietrying123]

*Potentially works*-we'll see if it works in about a month!

[@kamanashisroy]

@@conqueragingordietrying123 Did you check www.ncbi.nlm.nih.gov/pmc/articles/PMC3829447/

[@kamanashisroy]

@@conqueragingordietrying123 This study is saying long chain PFA like, Linoleic acid found in grapeseed oil activates Sirt6.

[@bobmciver6437]

Are there no measurements for the MUFA Oleic Acid which also activates ppar alpha?

[@conqueragingordietrying123]

Compared to PUFAs, oleic is a relatively weak PPARa activator. That data is in the Dreyer 1993 paper (in the video's description).

[@bobmciver6437]

@conqueragingordietrying1797 😊 Thank You.

[@adalllah]

As usual amazing insights 🎉 There is a big hype about 2 compounds 1- cardrine 2- (5 amino 1mq ,as energy boosters I have used cardrine with a patient who has heart failure EF 20 percent only and it was very useful for him Regards

[@hamidaabdenour9092]

5amino is so expensive

[@niallstallard5936]

Fish oils seem to have a lot of other benefits, I couldn’t decide which to take, so I’m going with all 3. Wild-Caught Salmon Krill Cod Liver oil ALA has other benefits, been meaning to add this in, now you gave me another reason to stack. Like you, I want to stay whole food as much as possible, already take a lot of supplements. As you are dialing things in, even if what you do may not benefit NAD levels, keep eye out for other benefits. Of course if something degrades your health, that would be something to drop.

[@conqueragingordietrying123]

Yep, definitely, thanks @niallstallard5936

[@jamesgilmore8192]

As I remember cod liver oil is high in vitamin A and you need to be careful with high or daily dosing.

[@conqueragingordietrying123]

Yep,@@jamesgilmore8192 that's definitely a factor, as are the additives in fish oil to try to keep it fresh (i.e. not oxidized).

[@jmvh8736]

Volume is super low, as usual.

[@conqueragingordietrying123]

Hey @jmvh8736, are you on a mobile device or PC? For me, the volume is ok, but I'm on a PC.

[@jmvh8736]

​@@conqueragingordietrying123I'm on a PC, I watch tens of videos a day and the volume of your videos is always very very low compared to others.

[@tedschwartz2142]

but fish oil is contaminated with heavy metals

[@conqueragingordietrying123]

Yep, that's a potential weakness of the approach

[@andrew33933]

try to buy labtested omega3s

[@larsnystrom6698]

@tedschwartz2142 Krill or algae oil won't have any problem with heavy metals. Although, I heard that fish oil usually has those removed. But I haven't verified that.

[@xjoy3920]

If you notice, all the experiments you do are associated with lipid lowering agents 😅 PPARs , Niacin.

[@jamesgilmore8192]

That's probably just a coincidence because the mechanisms are different and/or off-target. But LDL-C does increase with age....

[@tedschwartz2142]

what about fucodian ...it activates sirut 6

[@conqueragingordietrying123]

Got any data that fucoidan impacts NAD?

[@Blurns]

I understand wanting to avoid prescription medication, but avoiding supplementation entirely is just detrimental for most people.

[@conqueragingordietrying123]

I'm pro-supplements, but with a demonstrated need, and with regular testing to confirm efficacy. Unfortunately, most don't test at all...

[@JustJulia-qt9nh]

And the $1k question: is the pathway identical in rats and humans?????

[@conqueragingordietrying123]

Definitely-we'll see on the next test, as I've added fish oil to do this experiment...I'm open to doubling the dose, too, to test it 2x.

[@JustJulia-qt9nh]

@@conqueragingordietrying123 very cool. Looking forward to your results. 👍

[@kindedge]

Brill

[@WillowUfgood]

I really doubt increasing PUFA will work to increase NAD+..If that was a viable strategy then a modern diet would be healthy instead of obesity inducing. Lol!

[@conqueragingordietrying123]

That's ok, I appreciate doubt, it may not work. But, I'm not talking about PUFA from processed food or on an obesogenic diet. Within that context, NAD would still probably be low. The only way to know is to test, and I'll have data for this experiment sometime within the next month.

[@WillowUfgood]

@conqueragingordietrying1797 of course, I still applaud your self experimentation and testing strategy. But I worry that PPAR activation is compensatory - a way of dealing with fat intake - and that one biochemical reaction that produces NAD+ would not make up for increased fat consumption., which will cause other changes like to carb metabolism, for example. Also, PUFA is PUFA, and the source is largely irrelevant, IMO.

[@StochasticCockatoo]

what about.. cannabis

[@conqueragingordietrying123]

Does cannabis activate PPARa?

[@NoCredentialsNeeded]

Chug fish oil

[@conqueragingordietrying123]

I've added FO, we'll see on the next NAD test if it moves the needle

[@tedschwartz2142]

and i am vegan and i hate the smell of fish ..

[@conqueragingordietrying123]

Fish oil burps aren't pleasant, either. Linolenic and linoleic acids activate PPARa, so if it works in vivo, flaxseeds, chia, and walnuts could be good. The question is dose, though.

[@monnoo8221]

Such you are short of DHA and EPA almost for sure and quite significant. That is damaging all your high performance membranes such as in kidneys liver and neurons.. Perhaps the reason why you hate fish...

[@proximacentauri8038]

amazing channel