Indirect and Sandwich ELISA

[@ElnaKruger]

Just another big thank you from a biochemistry major for all your hard work! You're a talented teacher.

[@KJKP]

Sandwich ELISA starts at 7:08

[@HA-wn3gg]

you're amazing! I studied my last exam from your videos and I got 17 out of 20, the teacher marked my down 3 marks for 3 questions that I didn't answer correctly because simply you didn't mention it in your videos, all thanks to you I wouldn't pass the test

[@TTHPham]

Very clear explanation and straight to the point. Thank you so much!

[@jessym7965]

no worries, I worked really hard T.T.H Pham

[@human2951]

Sir sir sir..U r saviour of a biochemist.. Profound Respect from Pakistan...

[@philipphill9518]

Thank you for this very informative video. There is one thing I noticed though: the image on the bottom right shows the green detection antibody binding to the primary antibody with its Fc region, when actually it would bind with its Fab region. The Fab region is what gives the antibody its specificity against a certain antigen, whereas the Fc region is necessary for recognition by immune cells. Also, primary antibodies binding to two epitopes (antigens) simultaneously is dependent on the size of the epitope and (especially for IgG antibodies) not quite common. I hope I didn't create any confusion. Cheers

[@ferrerajones9314]

good catch!

[@NikhilGarlapati]

Yeah I was super confused by his drawing, thanks for clarifying! Also would each of the enzyme linked antibodies bind to two separate antibodies of interest because there are two Fab domains?

[@JustMe-zz7lw]

Love it. Thank you so much for being a kind person and explaining things for others to understand and not to show off how much smarter you are and rest so stupid. It is a rare quality in teachers to actually want to teach.

[@bhagyasreejm6824]

Lots of thanks .. you always boos our confidence. I always get excited to switch on your lectures

[@carolinadeluca7242]

Thank you from Belgium! You are very good! PCR, Plasmids, Polyclonal Antibodies, Monoclonal Antibodies, ... all those videos are amazing! I'm not an English speaker and you talk so clear I can understand :) ... Thank you !!

[@zannatulferdous9087]

The best explanations require no follow up questions. Thank you!

[@sydneysimukoko]

Thanks very much Dr, My immunology course is a always easy to understand when I listen from you!

[@MedicoDiscurso]

I appreciate this guy so much. Saving my grade.

[@sunnykimmy]

You are a gift. Thank you for your awesome, clear, concise and colorful explanations on these methods. :D

[@jessym7965]

no you are are gift kim.

[@anupray9117]

Great!! Very helpful to an Organic Chemist developing Biopharmaceuticals... God Bless You With More Energy!!!

[@qamar8655]

OMG I cant thank you enough ♡

[@AKLECTURES]

+YulYul Addict You're welcome! :)

[@GideonRotich-tk1tg]

I love your Teaching approach. I passed my Exams because of this online class❤❤❤❤

[@AnnaJeanine]

Thank you for this video! It must have taken a lot of effort to draw the board etc. very very much appreciated!

[@mirkov6850]

You are a living legend. Respect!

[@xorg1029]

Just want to say that this video is SO extremely helpful. Please post more.. you explain everything so clearly! THANK YOU!

[@tiewei1614]

Great lecture series 👍. Very clear. A minor point, in the indirect Elisa, the orientation of the enzyme linked Ab should be the same as that in the sandwich assay. It may be upside down.

[@kevindubrow8293]

Excellent. For medical students this is a Godsend

[@clairewolf6013]

You're seriously underrated! Thank you so much!

[@nwilczyn]

the opinion of the scientific community is that he is appropriately rated

[@summerbreezenight]

I love your videos. They are so clearly explained. Thank you.

[@JP-cp1dk]

Absolutely beautiful explanation on Sandwich ELISA! Thank you so much, this will help me with my lab reports! 🥰🥰

[@jessym7965]

i love you AK lectures

[@alysia9151]

THANK YOU , THANK YOU , THANK YOU!!! GOD BLESS YOU!!

[@jolienmangelschots]

Very clear! I didn't understand the Sandwich Elisa really well, but now I do! Thanks!

[@MedicSD]

That took a process that I was having difficulty understanding and made it totally understandable. Thank you so much for this and your Western Blotting video!!!!!!! My GPA thanks you as well!

[@woofox333]

saving my gpa like always thank you so much

[@linaenger3721]

U did such a good job in explaining this method! Thank you so much for that explanation! (Y)

[@Baldwinthefourth-1176]

Why are you such a legend?

[@constancapages]

These videos are great! You deserve more views and likes

[@tsgheredagebrehiwet8467]

Excellent lecturer!

[@meghnabose3303]

AK lectures are the best....best explanation to every topic...thank you for making such helpful videos...can't thank you enough😊

[@aihamzamar8329]

one of the best Videos

[@okajakifragment4358]

Wow you are such a good explainer and teacher.... Keep up the good work... Kudos to your explanation

[@julianatoledo3352]

Awesome explanation!

[@mayasaba9457]

Thank you so much for this perfect explanation

[@nareshbarik5384]

Thank you ❤

[@miriamalvarezramirez5038]

Thank you so much! I loved the way you explained all the concepts

[@sabasolhmirzaei8591]

Thank you so much for this clear explanation!

[@alimebarki5007]

Great explanation of the process, but it would have been better if u added more informations about the Enzyme-linked-Antibody and the relation between the substrats catalyzation and the bond between the 2 antibodies. thank you

[@putritheresiabancin7727]

You're amazing! Thank you for making this simpler.

[@sakshikumari4344]

Thank you so much it helped me for exams

[@حيدركرارذيابصبيح]

Watch this on the day of your exam….I see you! Staying up late trying to understand this Keep going!

[@rootsm3]

Great explanation now it’s clicking

[@elenaungureanu8919]

Thank you very much for this explicit lesson!

[@wilfridewilfride5516]

Thank you very much, so well explained and understandable.

[@LAisyourgirl]

So clear and concise! Thank you!!!

[@journeywithrahat]

Jazak ALLAH sir❤️❤️❤️

[@nameerahwajahat7494]

i love your lectures so much thank you!!!!

[@natalieyako6613]

Amazing explanation

[@cristinavall8587]

Thank you for your help!!

[@alim7532]

Excellent

[@medinlab141]

You are the best! Thank you sooooooo much

[@nevershoutnever80511]

thank you so much all the other videos I watched were so confusing but you made it so understandable!

[@astridsiregar]

Omg im so glad i found your channel. Thank you!

[@dyoomah17]

I spent the whole trying to understand this concept . just after I watched your video everything became clear ,thank you so much

[@brittanykaras7998]

I love you. You're videos are so helpful. I wish i could just take your class

[@nwilczyn]

he might not be able to wear that sexy athletic-cut v-neck in his class though, so you may be better off!!

[@monical2648]

the best explanation ever! thank you

[@sohamkonkar2895]

Killed it bro! Thanks !

[@princessandbluemoon]

Thank you for uploading this! Very concise and helpful!

[@Silhouette-Oregano]

Thanks very much for this helpful video.

[@Dana-rm8cs]

Wow ! This was very informative thank you

[@Countess_Samar_SprayArt]

This video is just what i needed! thank you

[@twiringiyimanaemmanuel9742]

thanks be to AK lecture ,sir you forgot to add stop solution especially HCL acid .but actually explanation was very fine.

[@heidihi547]

Brilliant!! Thank you. So clear.

[@musiclover2636]

Thank you so much for your videos!

[@BlackMamba-is8yp]

very helpful and clear as usual one question though I'm not seeing Direct and Competitive ELIZA did I miss it?

[@sondesmohammed8531]

You are the best really🦋

[@RenaissanceSTL]

Great Job!

[@freekruka3705]

this guy rocks

[@AltairKasem]

You sir are a legend. Thanks a lot.

[@jessym7965]

no, you madam are a legend

[@forexample7588]

Sir your oldest student, I passt my many tests bcz of u

[@elafalibrahim9828]

Very clear , thank you a lot !

[@johansmith5258]

Anyone know if all his biochem videos cover all the MCAT biochem topics? Please help!

[@Radosteto0]

I finally got it right! Thanks!

[@beenishkhan1590]

EXCELLENT 👍

[@cutielion_bunny1957]

thanks for the explanation helpd me alot !!

[@zeenatshahjada3113]

Very clear explanation ☺

[@MasayoMusic]

Is it possible that when we 'wash' the wells, there is a chance that the bond between antigen-antibody or antibody-antibody w/ enzyme, break apart, giving us a false negative?

[@pizzalover1790]

i’m taking A level biology, and this was confusing but thank you for this video!!!

[@pizzalover1790]

taking this again in med school, and i came back to your video… thank you so much!

[@ngyongchou3033]

Excuse me, may I ask some question, if for direct detection and direct adsorption of the antigen on the wall of ELISA we still test for the presence of antibody in the blood serum or we are looking for the presence of antigen?

[@zainabalkurdi6175]

Just continue Thank you so much

[@emadnashed5992]

Great thanks

[@astaganaga1906]

Thank you so much!!!!

[@jonathanao2141]

great video

[@paulocholaoloo2166]

Thanks alot

[@tihtinabirhan9214]

O my Goa! Really you are agreat lecturer!!!

[@Punzrox]

Saved me again. Now im ready for my exam tomorrow!

[@Vanu08]

😂😂

[@yewshien580]

thanksss ! very useful and detail explanation

[@noorclark2226]

you are amazing, thaaaaaankkkk you

[@mariakara4848]

u are the best 🌹

[@alexaovalles9504]

So good! Thank you so much!!!!

[@ronaldjamatia7119]

thank you sir. may GOD bless u

[@halani6929]

can the manufuctured antibody, with enzyme(green in n particular) cause change in colour incase a substrate is added to it, just in case the antibody is in its reagent bottle alone?

[@رعدشاكر-ج5خ]

U r the best 💜🇮🇶🥀

[@Rriitr]

I wish that I can explain information just like you

[@Bl4ckiii]

Thanks bro 😊

[@user-gc4wj9np4f]

thank you !