Introduction to Bioinformatics - Needleman Wunsch Algorithm

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Basics of sequence alignment
Topic : Global Alignment
Instructor: Dr. Hassaan Mehboob Awan
COMSATS University, Islamabad.
#Protein #DNA #Aligment #English #Urdu

[@marianagonzales3201]

This was excellent! Thank you very much for a very clear and detailed explanation!

[@vicentcaselles4807]

Very nice video, thank you Farhan Haq

[@haryank330]

loved eur explanation sir from andhra pradesh,india

[@FarhanHaqj]

Pleasure

[@creativewrite2444]

Thank you very much sir, tomorrow is my exam!! I will do it well❤

[@FarhanHaqj]

Good luck

[@Exploration_Avenue]

Very informative Dr Sab

[@muhammadjawadhussain8441]

from Lahore well explained great sir

[@FarhanHaqj]

Thank you dear

[@jiilaanhani311]

Thank you so much, you help me alot!!

[@khan-fn7if]

Thank u sir , This video was very fruitful for me And gave me a clear concept about Needleman Wunsh algorithm.

[@adamharb9233]

shoukran kteer hakeem

[@mahashahid3748]

THANK YOU SIR ... IT WAS INFORMATIVE AND EASY...

[@aasmaaslam8951]

Sir I have a question when we are finding T(i-1, j-1) why we put value of 4 and j=3

[@shrutikachaudhary672]

Good explanation.... Thanks 👍

[@bitta1997]

Thank you Sir

[@phatimadesu9710]

W Alaikum AlSalam. Thank you

[@alishbaejaz4964]

Thank you sir!

[@kishwersodozai4342]

Well explained JazkAllah

[@m.scholl1001]

Entry (3,1) is -3 not -5. The value to the left is -3 + 0 (gap panalty). The Entry (2,1) has no gap.

[@alaaaref3131]

thank you this was very helpful

[@sabaaltaf4567]

Thank you very much sir. Best explanation i have got so far.

[@syedsalehhaider415]

I have a question, If all the three values from (Upside, Left, and Diagonal) are the same then during traceback what do write in the table either (Up, Left or Diag)??

[@FarhanHaqj]

All three. After doing traceback you can draw all three possible alignment and select the best one if you want!

[@nurulizzahmohammadnazir6285]

I've been looking for confirmation in this thing for such a long time 😭 If all three are right, which one is considered the best one though?

[@dillid-8894]

@@nurulizzahmohammadnazir6285 could you find the answer

[@nirjhar9725]

Very good

[@wonderfulIndia1]

Sir global needleman ek hi topic h or smith or local alignment ek hi topic h plzz reply sir I am very confuse now

[@FarhanHaqj]

Yes

[@olaf466]

thank you for the information it's much clearer now in my mind

[@aroobazahid2892]

For T(2,0) value is -4

[@savagebunny3194]

-2 + (-2) is not 4

[@anishav.s9049]

your explation was very useful and i got a clear idea about it thak you so much, but i have a doubt that how can i calculate the score in needleman and wunsch algorithm when i perform an alignment in that tool

[@gabrielsamora9351]

This video is saving my ass from failing my Bioinformatics exam Thank you so much

[@mehwishrehman9787]

hello sir, I have a question.. how to solve the trace back step if we get 2 highest common values i.e. one in the first box of top right column and second value in the second box of the right column lets suppose I have 2 as my highest value.. and it's present in both the1st and 2nd box of extreme right column then how to trace back?

[@FarhanHaqj]

If you are solving needleman then getting the maximum value in the top right column is wrong, since first row of the matrix contains only GAP values. Gap values are always in negative. Secondly, if the alignment is correct, then regardless of the maximum value, traceback of needleman starts from the bottom right cell.

[@user-xz2dz8zp6r]

T(5,2) arrow from T (4,2) only coz it's a mismatch

[@rajputanjalisingh1538]

Can any one explain the value of T(4,2)... What's the answer?pls ...

[@aryakrishnanls6745]

-4

[@pristineeducation6815]

An excellent explanation

[@jawaheralhuthali]

thank u

[@user-xz2dz8zp6r]

You didn't calculate the optimal alignment

[@MA-ny2gc]

please say something on gap penalty determining

[@Bint924]

Mashallah well explained

[@moetasembellakhalifa3452]

When finding the Levensthein Distanz do we need to use the same scoring shema in the matrix? or can we use a different scoring shema after we have found the best alighnment?

[@zahramasood6846]

Is it complementary to put arrows?

[@FarhanHaqj]

Helps to traceback and build alignment

[@FarhanHaqj]

Ok

[@sautrikbhattacharya]

If the both sequences are not of equal length then how to place them in the matrix?

[@FarhanHaqj]

Two sequences donot need to be of same length. Row and column can be of variable lengths.

[@sautrikbhattacharya]

@@FarhanHaqj ok but both both the sequences will always start from i1 and j1 respectively, right? Or can there be rows and columns left blanks at the start?

[@FarhanHaqj]

There must be always one row and one column blank in the beginning of the matrix

[@A2S_ArtsToScience]

Why j=3?

[@user-xz2dz8zp6r]

T(1,4) again arrow comes from T( 1,3) coz it's a mismatch

[@azizbaloch7897]

Plz help me in my assignment vu bif 401 spring 2021 I want to understand then do myself there are lots of solutions but I want to understand very well then do myself plz help me sir

[@ruthboniface6911]

be blessed

[@azizbaloch7897]

Salam sir plz explain formula which is s1(I), s2(j)

[@ayush9462]

Bro it's similarity function. Duh

[@innocentgirl5923]

Penalty?

[@rajputanjalisingh1538]

Rest of the not match with the answers

[@Jayesh-w-005]

Jay shree ram🚩🧡

[@maheshpatil6653]

Work on your speaking.....

[@lamaayoub8420]

bhebak bas hek

[@anaranyabose]

Bad explanation since you just showed us the process but not the reason behind this.