Introduction to HPLC - Lecture 4: Ion Pair Chromatography

[@RazaKhan-ds8by]

He is just star. He is brilliant and there is no praise for his work. So simple and well explained.

[@ChemComplete]

Wow, thank you!

[@SrikanthSC1408]

Loved your lecture....Now I am confident with HPLC Thank you so much

[@cinthiaiscoa3107]

I was searching for an explanation about HPLC IP and I am now aware about what's going on inside that column haha. Thank you so much!

[@madispalmet9905]

Great video man, very useful information!

[@soheylasiavoshi7759]

I love your explanation! Thanks ❤

[@Ambient_Scenes]

Thank you so much for this great lecture!

[@maxaugusto1958]

Great material! Thanks so much.

[@AbdullahAlajrame]

Hi Dr thanks for your time , it was too much excellent explain for IPC

[@shgamer8178]

I’m waiting for next one

[@thepositivitypill9419]

My boy at it again!!

[@lecongminh760]

Thank you sir. I hope you will guide us about some problem in operation HPLC and the trouble shooting (ex: high backpressure; strange peaks,...) How to wash column to assure the analysis result. How to check all the HPLC system daily,....

[@paritawanjari658]

nice lecture series.....

[@toquyentruong1530]

Thanks for your lecture, Sir. I have one question about ion-pairing reagents. I am setting up a method for sinigrin (target compound) detection. In the reference method, authors used TBA (hydrogen sulfate-SO4) reagent but I don't have that chemical. What I have is TBA (hidroxide-OH). I am wondering whether I can use TBA (hidroxide-OH) pH 7.5 adjusted with phosphoric acid to replace TBA (hydrogen sulfate-SO4). I am looking forward to hearing from you soon.

[@rreyescastro]

Please do normal phase and make it available to buy!!

[@pathumchandika769]

thanks for the lecture !!!

[@Ambient_Scenes]

May I ask one question? Is this the best choice for separating amine salts (e.g. phenethylamine.HCl)? Or could this maybe achieved with a C8 column with polar end-capping together with a non-polar mobile phase (e.g. hexane + THF)? Might it even work on a C18 column, even though the amine salt will interact with the hydrophobic tales even less? I guess to take this question even further, are there columns that could separate both freebase amines and their salts, assuming one varies the mobile phase accordingly? (and maybe during sample prep, separate the sample by pull the salts into a polar layer and the freebase amines into a non-polar layer) Sorry for the rant but for some reason I can't find the answer to this question anywhere...

[@bseo927]

Thank you so much for the lecture! I do have a question. I do know that once you introduce IPA to C18, that column becomes dedicated to IP RP as you mentioned. But can I switch between different alkylamines in order to determine the optimal IPA for my negatively charged analyte (acid)? For instance, maybe I start with triethylamine, do some separations, wash the column with ACN without IPA, then introduce DIEA or hexylamine for my next test. Or do i need to buy separate new C18 columns for each IPA? I only need to use alkylamines as I do not need to analyze positively charged analytes.

[@akbarbahrami985]

Greatttttttttttt. Thank you so much

[@mhamado.abdullah6925]

Thank you, amaizing

[@madisonhartz7862]

thank you so much! :)

[@randomvideos5704]

Release the next sreies plzz

[@joshtrent7945]

my first year biochemistry prof should be giving me a refund...and an apology

[@ChemComplete]

Teaching methods can make a world of difference. I hope you found the lecture helpful!