Introduction to HPLC - Lecture 2 Column Chemistry

[@yoanagendzhova691]

I have a Phd in chemistry and have used both flash chromatography and hplc and still learned a lot from this video thank you!

[@joshuablank8687]

Awesome lecture! Super informative! Are there still plans to continue this series?

[@alexandermelchor9362]

I've been waiting for lecture 3... who knows when he'll post it!

[@ChemComplete]

It is in the works!

[@joshuablank8687]

ChemComplete Great! Looking forward to it!

[@xiaomanzhang3038]

Very informative! It's very helpful for my interview. Thank you sir!

[@joshr.5199]

Thanks! I’m applying for a job and I don’t have prior experience with xD

[@rinnemollema5826]

Without a study?

[@madihasamar]

can you please make a video on ''reading chromatograms'' and calculations based on different results.... etc

[@Anmol-bf3ml]

I wish I could give you more likes :)

[@johnnycoleman4087]

Taylor Donna White Helen Jones Thomas

[@rc7314]

Why are the C18/C8/C4 columns the most common alkyl groups? e.g., why aren't C17 or C9 or C5 columns as common?

[@sheenalishaukatali3109]

Kindly upload gradiant HPLC sytem

[@titansfolly2660]

These are really good, can you do a video series on Gas Chromatography?

[@ChemComplete]

I have an intro GC video already but it is oriented more towards undergrad labs and lab report writing. Down the road I can consider a more formal series like this. GC will follow the same general premise but you will be looking at gases carrying analytes instead of liquids.

[@smaila5108]

Hi sir,is there any expiration date for Hplc columns? Thank you sir.

[@ChemComplete]

If factory new and packed not usually. Most columns (especially c18) tend to be fairly stable. However is a column is open its lifetime can vary by many factors and does not guarantee a given number of runs. What you are separating, solvent choices, and proper column maintenance (not leaving water in columns for extended periods) are all important.

[@smaila5108]

@@ChemComplete thank you sir.

[@youtube_user9110]

Some columns for SEC analysis from General Electric do have a Best before date. Specially for columns that can be kept on the fridge.

[@smaila5108]

@@youtube_user9110 thank you.

[@emilieroostkristensen7601]

Thank you for your educational videos! Could you consider talking about pressure feedback in one of your future videos?

[@ChemComplete]

In general pressure feedback means a column issue. If pressure is too high it is often a result of columns with too much residual material build up (older columns) or a mismatched column (pore size too small creating back flow issues). Some solvents can also cause issues, especially if they are viscous and thicker.

[@aaalexandra.marieee]

youre the best. Helping me so much

[@Liamallica]

Thank you for the content, v useful for brushing up. Just a small correction if anyone else is confused. ~ 30:45 mark looks to be a fronting peak

[@TanveerAhmad-hw6ds]

Plzzz upload all HPLC AND GAS CHROMATOGRAPHY lectures from D C HARRIS BOOK

[@weronikajasinska4351]

Your lectures are absolutely fantastic! Thank you!

[@dirkhoekstra727]

What columns and mobile phases do you use for analyzing berries for well-known pesticides?

[@giridhargopalsingh6887]

Please guide us with type of columns in L1 series please

[@noureddineraghdi4340]

thank you so much...Please do you know how a column is made???, I mean how to fill it with silica, despite its very small size. Please if you have a pdf put the link here

[@tranll1]

Thank you so much!! Very informative lecture!!

[@ChemComplete]

Glad it was helpful!

[@96Milena]

Thank you for the great video! Does the lack of end-capping cause tailing or fronting in the peaks obtained? :)

[@jacobtrombley7650]

I think it would cause tailing

[@ChemComplete]

Tailing

[@amornratrat]

Thank you very much.please teaching LC-MSMS.

[@mhamado.abdullah6925]

Thank you

[@josephomondi629]

thank you for these videos. very informative

[@naeloveee]

that was a good lecture thank you

[@muhamadmustafa5218]

Thank you so much

[@amolgawande9248]

Very informative ...ty

[@soheylasiavoshi7759]

Perfect❤

[@ergrthdfhdh3935]

great

[@04shakhawathossain75]

Thankuu

[@javiersevilla8790]

Thank you so much, your videos are incredible, greetings from Mexico!