Introduction to Illumina Sequencing

[@muhammadakmal1414]

Respected Professor, You nailed it. Alot of love and respect you from Japan.

[@tinac4965]

finally an explanation that makes sense thank you

[@hediteymoori9231]

Hello I cannot undrestand the limited cycle PCR in NextraXT library preparation. the first temperature that was shown after tagmentation is 72 degree . Is it related to the extension of the 9bp of tagmented site by DNA polymerase?

[@annieasghar2587]

I have one question... your DNA is sequenced by ilumina miseq is of 5MB size. U have to get 10x coverage . How much data do u need?

[@michaeltolkachov4749]

Concise and helpful, thank you!

[@jennyjenjira4572]

How did this professor write in reverse manner?

[@JumKalif]

The board has a mirror which allows him to write straight and it shows straight on the viewing end

[@ashleyj9326]

thanks!

[@Yukanforward]

its cool

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH