Is mushroom senescence real? The truth about mushroom strains and gene banking mycelium

[@bwandonmelon6333]

Your videos just get better and better over time gary. Keep up the inspiring work brotha! Mush love

[@FreshfromtheFarmFungi]

thanks for watching and following along!

[@johnstamos4629]

This was SO fun to watch. I LOVE the scientific method applied so meticulously then end with beautiful mushrooms. You and SouthWest mushroom's taught me all the sterility work and how to grow cultures on various mediums. Now I have grown around 15 different kinds of mushrooms. Im kinda proficient and knowledgeable thanks to you!

[@danielmcardle3476]

New fan here saying a big thank you. You are a true scientist sir! I'm just getting started and your videos are really inspiring and educational. I hoe you have some good detailed videos on lab technique as I am struggling 😊

[@FreshfromtheFarmFungi]

thanks man! We have lots of lab videos a lot of our fungi friday live streams show technique very well and our breeding series 🙏🏻🍄❤️

[@sacredatavism]

I'm just now seeing this, but I just wanted to say I appreciate so much that you did the work. There's a lot of old "wisdom" that gets repeated uncritically by people, and it's important to test this stuff and see what holds up and what doesn't.

[@FreshfromtheFarmFungi]

yes one day I wish to revisit this topic with more resources and do genetic analysis but objective tests work well too! thanks for watching and following along!

[@InknbeansPress]

Conclusion 29:12. If you're a small hobbyist...30:22

[@hesherette]

this was awesome, thanks for making the extra effort to take us along on your experiment! this was so cool to see laid out so in depth.

[@FreshfromtheFarmFungi]

thanks for watching and following along! 🍄❤️

[@thecakeredux]

Sick man, thanks for doing this. I've been thinking of making an experiment like this, but it's so, so much work and I'm super grateful you took that onto yourself. Cheers!

[@FreshfromtheFarmFungi]

thanks for watching and following along!

[@thecakeredux]

@@FreshfromtheFarmFungi Of course! Quality stuff!

[@ChippyTrance]

Gary you are one of the best. Thanks for your effort man

[@edwardgrand]

Nice experiment and video. Why do you think hybrids would be more susceptible to senescence?

[@FreshfromtheFarmFungi]

thanks! It’s just a hunch because of how sparse the mycelium can be sometimes with the black pearls but it is just an idea I would love to have more access to genetic testing I just am waiting for other projects before I can dive into R&D

[@edwardgrand]

@@FreshfromtheFarmFungiI thought my mom-mon and di-mon crosses looked a bit weak, but after 2-3 transfers, they look just as strong (if not stronger) than my standard cultigens. I'm not sure how we could test this other than just fruiting them which is problematic for the typical cultivator :(

[@MissBlackMetal]

I was literally holding my breath while watching you cut and transfer the Lion's Mane mycelium on those contaminated plates 😂 I'm really glad you showed that process though, actually. If I'd have seen a plate like the second one you transferred (where there were 3 spots of contam very close to the mycelium, and the mycelium hardly grew out at all), I'd have assumed it *wasn't* salvageable -- so it's really good to know that it is still worth trying!!! I appreciate you! 🤗

[@FreshfromtheFarmFungi]

yes thanks for watching! It’s always worth a chance to me 👍

[@meadguysj]

Thank you for taking the time and effort to produce these videos. I've struggled with the whole 'senescence' concept over the past 4 years of growing edible mushrooms. At least your video provided some suggestions and guidance. I'll have to purchase and try growing some of your Pioppino liquid culture now. And I agree with other commenters about the music. - I have to turn up the volume to hear you talking in your clean room. - But then the music is way too loud, so have to turn down the volume. It's a bit tedious. But I'll continue watching your videos.

[@FreshfromtheFarmFungi]

thanks for the feedback! I will work on dialing in audio it has been a fun journey learning editing from the beginning and now I can work on the smaller details 🙏🏻

[@DawidKellerman]

Thank you for the Effort and Work!

[@marvin216]

Thank you Gary, you are a true 🐐 of the community. Mush love!

[@FreshfromtheFarmFungi]

thanks for watching and following along on the journey 🍄❤️

[@TrevorGspivey]

some good old fashioned science. Would love to see this done with different varieties of cubensis mushrooms

[@ianthebastard8128]

This is a fantastic video Gary, we have found that a couple of our older strains are not quite what the were, BPK being one of them... I like that you have used two different grains with different results from each. We've only ever used summer wheat because its easy to get, although the wheat has never been an issue, it might be time to experiment with some other types, oats shouldn't be too difficult to find. Much appreciated.

[@FreshfromtheFarmFungi]

thanks for watching and happy growing! 🍄❤️

[@official-kin]

Inhailing all these quality videos from you and always learning something! 👍 I got one question.. i have got some Dimethylsulfoxid for kryoconservation of liquids. Do you think it is possible to prepare some Tubes with distiled water, dmso and some fresh mycelium in it and freeze this for Long Term. Will the mycelium revive? Thank you so far, Garry!

[@andreash.9615]

How do you store mycelium long term? Can it be frozen?

[@Greenkillr]

A lot of work went into that; appreciate it🤙

[@DjMannyb]

Great video! Thanks for sharing. I have a question about your grain spawn lids.. I used the same syringe filters for gas exchange, but I found that the inoculation was much slower than micropore tape.. and in the case of larger jars, the mycelium would struggle to get to the bottom of the jar. It could be that I got dud filters.. But I also noticed that you have smaller jars. Have you face this issue (which is maybe why you are using these lids with smaller jars?)

[@FreshfromtheFarmFungi]

I think the air exchange is less important at this stage and genetics play a larger role. The reason I use smaller jars is so I can do lots of variety at once - larger jars means less jars and less different types of

[@petrabraham9512]

What's the name of the music that starts at 17:50?

[@renegarza1436]

What kind of jars are those and what size? I like them!

[@FreshfromtheFarmFungi]

they are half pint ball jars wide mouth - here: like these amzn.to/43xUtWZ I got mine from walmart

[@GDB_2020]

That is so good! One question, how do i get a mother culture and not a clone? I need to cultivate from spores?

[@FreshfromtheFarmFungi]

this would come from a successful mated pairing of two spores - you might want to grow the first batch from spores, select the best mushroom from there to clone and consider this the mother

[@GDB_2020]

@FreshfromtheFarmFungi thanks for the info, was confused about it, there's a lot of misinformation out there.

[@bmartin2304]

Hi Gary. I'm getting set up to start growing lions mane in a 4x2 grow tent(XL martha). I really appreciate your content, I have learned a ton. for some reason I still can't grasp the preservation procedure. Let me know if I'm right or wrong here, say I order a clean culture from you and I want to preserve it for years to come, should I sub that culture into many slants and keep those to try and only pull from one at a time saving unopened ones for following years? or sub culture it into a few different storage types, LC syringes/slants/agar and sterile water so I have back ups if one method doesn't last? one more question, when you start from spore from a cultivated mushroom, are the traits then random and your start over trying to find a good phenotype, or do some of the genetics get passed through the spores as well. thanks.

[@barrypeters5136]

I had a similar question. Found a thread on line that said even once a fungi has seneced, getting spore from the fruit of said fungai can still produce a very stong genetic strain as there are so many variations. Also if "female seneced spore genetics crossf non-seneced "male" it can produce more seesed strains. Inversely a non-senesed "female" crossed with a senesed "male" produces more non senesed strains. I'll see if I can find that paper. Think I read this to be true for yeast and the like but not sure if it also pertains to mushrooms.

[@bmartin2304]

@@barrypeters5136 Ah, cool. I appreciate the info. If you find it I'd love to read it, thanks!

[@esrcornwell]

Would love to know how many expansions you had between the years of the slants. Like was 2020 lions mane made from the 2019 from just being plated once and then slanted? Or was it ten or twenty plates later?

[@FreshfromtheFarmFungi]

It was 2 or 3 plates and an LC culture for every season and the slants were original

[@esrcornwell]

@@FreshfromtheFarmFungi wow not a lot of expansion from a slant to two or three plates back to a slant and already starting to show senescence. Super interesting! Thank you for doing this experiment so I don't have to 🙂

[@ehbarooo]

Very interesting nice video ! Could starting from spore reverse the senescence ?

[@FreshfromtheFarmFungi]

I think it is one of the ways yes

[@MrOiam]

a little behind, but the cultures following the original were obtained, how? thinking tissue culture to agar, or LC (creating the generations)

[@FreshfromtheFarmFungi]

I usually start from the mother slant, go to agar plates to verify cleanliness, then go to LC. Then start back from the mother culture once the LC is finished which is usually around a 6 month time span.

[@shagdawg420]

good shit

[@FiliFili-bf9mn]

Amazing content! Thank you!

[@BStott]

Very confusing. Shown 20-22 Lions Mane spawn much more aggressive in spread. Yet, you tell us the 2019 fruited way ahead and was on secondary flush with the 20-22 hardly fruiting. Did you mix up the video and your remembering the growth? What slowed them down if not a mistake on identifying the years?

[@FreshfromtheFarmFungi]

no it was spaced out because of my grow room size- I wish I had a larger sample to run. So the 20/22 stalled while fruiting but the 2019 fruited very quickly post colonization- hope that clarifies things. It was a few days difference and I also could not account for temps. I will repeat this in the future with more controls

[@flaparoundfpv8632]

Hey Gary, do you grow cordyceps? Do you buy all your master from a breeder, or do you have a breeding program of your own? I would like to hear your opinions on senescence with cordyceps, and whether there is any other way to produce without having to rely on a breeder.

[@FreshfromtheFarmFungi]

Thanks for watching I do a few batches of cordyceps a year and have been building my own library for some time now off of cultures gifted to me over the years. It is very interesting how they behave and from my experience they seem to degrade very quickly compared to other mushrooms. I have a few videos breaking down our process look up the latest one here kzbin.infoo2aifWsy68s?si=9RcWF6yFrXg-tHqc

[@GrayDelmar]

Good stuff Gary!

[@FreshfromtheFarmFungi]

thanks for watching and following along!

[@andrewmodell5889]

So your wrong that term refers to the blueing Hey Mrs the name of a color ..and Paul Stamets childs name... 2:07

[@Foxxxxx96]

is it normal for mycelium in slants to not grow as rigorously as it does on plats? All my slants (both on mea and pda) seem to stop growing about halfway through...

[@FreshfromtheFarmFungi]

yes if you restrict the fresh air it will usually stall out

[@ivanuruguay2011]

Hey Gary, nice video, thanks, I have found in my little production tests a lot of inconsistency between blocks inoculated at the same time using the same LC, maybe this is a problem for me and not for you, a strain issue maybe, but still I wonder if the different results you are getting here in your experiment may be the result of random block production or inconsistency between blocks rather than senesence, maybe you would require ten or even more blocks from each culture to have a higher accuracy and avoid error due to inconsistency, just to comment, thanks again Gary, awesome work

[@FreshfromtheFarmFungi]

This is true which is why I would love to test the genetics 🧬 it is the best way to see the changes at a quantifiable level

[@eve_squared]

how many agar plates did you transfer from spore plate?

[@penguincqh]

If i get spores from the senescenced strain's fruit and breed a new strain from there, will the genetics gets refreshed or their offsprings will also be genetically weakened?

[@FreshfromtheFarmFungi]

I think there is a chance it could be reverted because spores are not the same genetics as a cloned copy and if you were lucky enough to get a superior genetic from that breeding process then it would be totally different then the parent. This is just speculation though I think the key to understanding more will be genetic testing and comparing sequences.

[@ionizedscience296]

Is it a mutation, Or phenotypic plasticity to its nutritional environment and atmosphere conditions

[@FreshfromtheFarmFungi]

my experience is that it is a mutation since if you clone this, go back to culture and refruit it will carry these traits again. I think phenotypes express themselves once during a flush and can vary throughout the grow room but if I go back to the mother culture it will give similar results.

[@christopherholley3820]

Thank you

[@JoelLongtine]

Where do you get your grain from here in Colorado?

[@tamertamer7082]

I always please to watch your videos ..definitely prolonged propagation of secondary mycelium will lead to mutation accumulation that is why sexual structure should repair these genetic disorder

[@J155P]

Not sure I understand how to maintain a mother culture. Sweet experiment 👌

[@jamestunnicliffe1]

If senescence is real better to just start over again from spores?

[@FreshfromtheFarmFungi]

it may or may not be better but it is important to keep mother cultures from the newest generation in storage

[@joalsoal1645]

I wonder if the spores would look any different under the microscope?

[@I_was_Charlie]

cool

[@SachaLotus]

Great video 👏🏼 . Don’t forget to drop a like 👍🏼

[@marsjuzeew1286]

Try growing truffles in a plastic bag

[@marsjuzeew1286]

@hopebeel8765 kzbin.info/www/bejne/l4e7foCdopyboNU

[@mikecorcoran6834]

Expert is a relative term. Compared to me, you're Einstein. ❤

[@protodank]

Letting out all the secrets! Mush luv forreal!

[@AmeliaRichard-gw3oh]

Very spiritual, brings a lot of truth to the light and makes outside look really beautiful. I enjoy them, It’s like a natural depression blocker. Bought Them from an online drugstore where I sourced mine

[@AmeliaRichard-gw3oh]

MYCOTEEP

[@AmeliaRichard-gw3oh]

On IG

[@PluckMe]

Oh my Lord. You all have the same problem with the music. Trying to hear you over the necessary sounds and then y'all think the solution is to add another competing sound into the mix. Because we don't come here to learn something we come here to hang out and get wiggy with it. FFS Right here where the music starts is where I skip ahead to see the outcome and I'm out. SMH