Pheno Hunt 2022: Selective mushroom breeding

This video is the complete walk through of our Immaculate Inoculation pheno hunting series. We selected for the fastest, healthiest, largest yielding mushroom among 38 different phenotypes. To watch the complete series visit the playlist "Immaculate inoculation" • Immaculate Inoculation...
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[@joshp5737]

37 minutes.. Gary's doin Full Length Motion pictures now y'all LOL jk Gary thank you so much for this vid Gary and for all your other ones that you've educated us with over the years.. i owe so much of my success to you.

[@FreshfromtheFarmFungi]

thanks for watching! Trying to improve each video as they come out 😁🍄❤️

[@JustinLietz]

Thanks for all the priceless content Gary, you make the most thorough videos on practical mycology that I’ve seen

[@RenegadeMushrooms]

I very much appreciate all of your hard work on the genetics end. Can't wait to try out those Mach 3s.

[@FreshfromtheFarmFungi]

thanks! Looks like you are doing some good work yourself over there! Glad to put this one out there it’s my favorite so far! 🍄❤️

[@terryrogers8503]

Love your strains, your breeding is top notch. Way better than anybody elses breeding.

[@FreshfromtheFarmFungi]

thanks! I have some big plans 😁

[@terryrogers8503]

Your on the right track, don't stop

[@KennedysMushrooms]

I know I'm almost a year behind watching you work on these crosses but I couldn't help but smile over and over watching you with your Mach 3! You've had me on the edge of my seat with expectation. Very well done and thank you!

[@claychambers82]

Thank you so much for walking us through your process!! Looking forward to the 2023 edition!!

[@FreshfromtheFarmFungi]

thanks! Yes I have some plans to scale!

[@localbroadcast]

speedy OG! Awesome work /w your breeding work going above n beyond the call of duty!

[@slipknott9]

Just Bought the Mach 3! Can’t wait. Thanks Gary

[@maroosk]

Great work, been awesome to follow, welcome to the m1 and m3! Love it, what a project!!

[@xspells]

Dude Garry u rock man. Uve inspired me and helped me grow as a student ty

[@davidmorrisii69]

Im in like 10 mushroom clubs, and u are very, very smart, keep it up brother!!!@

[@MyTube4Utoo]

Very interesting. Thank you.

[@curiositeconstante404]

Your the best, I'm definitely going into hunting phenotype, thanks for all your teaching !

[@o0tdaigle0o]

So thankful for this kind of content. Great work Gary!

[@davidmorrisii69]

Yo! Whats up gary??!!..., its lil dav from ludowici, Ga!.

[@Sludge73]

Beautiful product pics at the end. Love the content. Great stuff. Thank for sharing! Can't wait for a back yard Morel update. 🍄❤

[@FreshfromtheFarmFungi]

same! It has been on hold because of the freezing nights but Friday I am turning on the misters 😅

[@philcordeiro288]

Got me some Mach 3!! Can’t wait!!

[@user-jn7nf6xu8l]

you are good Gary

[@glennplatvoet7111]

Nice i like the set up looking good 👍 I'll keep up i think w is going to work out the moch 3 good. Hope it's tasty

[@joshp5737]

Hey Gary, I just wanted to chime in 1 more time to let you know about this experiment i did with peptones.. i added peptone to the water when i was boiling my oats.. after i bagged the grains up and inoculated them , the 3lb bags are almost fully colonized after 5 days...much faster than normal. Cheers bro

[@FreshfromtheFarmFungi]

awesome! Thanks I would love to dive deeper into spawn additives 🙂

[@thegraz]

Gary you are a Denver treasure, keep up the great work

[@SkidMarkSmeller]

Do lions mane next!

[@erik_carter_art]

Have you considered doing this experiment with each isolate in triplicate/quadruplicate? You could do it on a smaller scale so it's not wasteful, but it would help a bit with any variability that may be unrelated to the isolate biology so that you draw the correct conclusion of which isolate is best.

[@FreshfromtheFarmFungi]

yes we have expanded them out accordingly - more space is ideal

[@ivanuruguay2011]

nice job, thanks Gary, I have a question, you think it is enough with one block from each?, I found so much variability between blocks even when I inoculate the same strain

[@thomashugh7086]

Amazing work Gary thank you for the content. I do have a question tho. How do you manage your labels to keep everything straight?

[@FreshfromtheFarmFungi]

just make sure you label as you go and before you switch strains and pay close attention - this was a huge issue in my previous jobs because of the stakes of lab testing so it was engrained in me hah

[@RALEIGHthe3RD]

What would the expected genetic outcome be from spores harvested from a specific phenotype? For example, would M3 spores produce only M3, or all phenotypes?

[@jevonte-vb2pu]

wow

[@ClemMorton]

10:10 100% Humidity? I joke but, Isn't that like underwater?

[@sinhnguyen4815]

Don't touch the filter patches.

[@Akasha_Silver]

Nice. How do you wash or clean that fruiting room out?

[@FreshfromtheFarmFungi]

kzbin.info/www/bejne/gGOmfIKHi9eifZIsi=UZvKBTYMSY_24r6r

[@BoroBootBoy]

I need to look into doing this. I have had so many poor fruitings lately. What were your colonisation times on the oysters Gary?

[@FreshfromtheFarmFungi]

2 weeks from spore to clean culture, 2 weeks on grain, 2 weeks incubation, 5 days pinning, 3 days first flush, 19 days second flush (1 1b and .6lbish for flushes)

[@jeffreyhalverson2611]

Love ALL your stuff, Gary:)

[@FreshfromtheFarmFungi]

thanks for watching! MUSHLOVE

[@SkidMarkSmeller]

Can you breed a really red or pink oyster mushroom?

[@flopilop4596]

Hello I am a soon to be Uni student but I cant decide on what field I want to go into, microbiology/ biochem has caught my eye and I want to give some basic mycology a go. May I ask what injection filters are for? Do they have in this case the same use as micropore tape, to regulate air intake aseptically? I Cant find a conclusive answer. Since I have not that large of a budget and since I cant find a pressure cooker/pot with a pressure gauge in my country... a lot of my budget has been eaten up by that, I was wondering if injection filters and micropore tape interchangeable at the cost of chance of contamination? Are both micropore tape and injection filters necessary?

[@FreshfromtheFarmFungi]

yes it filters air and helps to filter air if liquid is being displaced in a liquid culture as well

[@johnstamos4629]

Were those bags still warm when you inoculated them? Did you mix the 2 types of pellets before inoculation or did you figure you could mix it at the time of inoculation?

[@johnstamos4629]

Weird how it can differ from flush to flush. My first, had a bunch with cleft caps from growing in tin pans and as the rust and mycelium color got worse, the second flush came out nicely. lol!

[@FreshfromtheFarmFungi]

they were about 75ish degrees and I didn’t premix the pellets only after inoculation

[@johnstamos4629]

@@FreshfromtheFarmFungi awesome thanks!

[@2010DERT2010]

Once they start to colonize, do you still have to sterilize your body and stuff?

[@FreshfromtheFarmFungi]

yes it helps during all phases to keep things clean

[@2010DERT2010]

@@FreshfromtheFarmFungi thank you

[@thematrixcentre]

For the last one and half months i just can't make spawn without trichoderma infecting the grain. Make spawn from tissue culture only, doesn't use agar or lc. Need some advice.

[@FreshfromtheFarmFungi]

it’s that time of year - this month and october is really challenging because the normal spore load is 10x or more - my advice is up your sterility standards (mop the floors, clean the workspace, add filtration) or wait it out 👍🍄❤️

[@thematrixcentre]

@@FreshfromtheFarmFungi thanks!

[@Foxxxxx96]

How are the slants packaged for international delivery on etsy?

[@FreshfromtheFarmFungi]

we heat seal them, send them with appropriate paperwork in a bubble sealed thermal envelope

[@alexportlock891]

I live in Brighton and I just found a oyster mushroom do you want a clone or spore print

[@FreshfromtheFarmFungi]

maybe a culture if it’s clean 👍🍄❤️

[@mitchhutto2953]

Like to have that machine 3 if possible

[@FreshfromtheFarmFungi]

I think there are still a couple slants left on ETSY etsy.com/shop/freshfungi

[@mitchhutto2953]

@@FreshfromtheFarmFungi 100 bucks man I'll pass but I still like ya videos

[@jeremylastname873]

Is the “blob” the PE of oysters? 😂

[@FreshfromtheFarmFungi]

hah it surprised me

[@o0tdaigle0o]

Ha, I was thinking that same thing. Could make a baby king, or prince mushroom. Something very unique.

[@jeremylastname873]

@@FreshfromtheFarmFungi I wonder if it would tend to be relatively fibrous. Of course, proving it out as a cultivar (vs an anomaly) might be more of a labor of mush-love than a profit-making endeavor.