Tasting Resiniferatoxin (RTX): The Spiciest Substance in the World | Full Extraction from Euphorbia

[@LabCoatz_Science]

Alright, could we all just take a moment to appreciate how much of that habanero I managed to eat? Also, quick note: I know the stuff I tasted wasn't pure and that the vanillin stain test wasn't highly conclusive. The plant I used was dying and kind of on the weak end (the latex didn't really irritate my skin, but it did taste spicy), so there wasn't a lot of RTX to begin with. Plenty of the resiniferatoxin was probably also lost to thermal decomposition and oxidation. But make no mistake: I did taste the resiniferatoxin in my sample. It was quite hot, despite my somewhat calm reaction, and there are no other compounds in resin spurge that create the burning sensation that I felt (irritation, yes, but not burning). Hope that clears up some of the controversy!

[@Painandmisery6860]

Is it cuss if u eat enough it kills u???

[@SetOfAllSets]

if no one's ever tasted it how does it have a scoville rating?

[@LabCoatz_Science]

@@SetOfAllSets People have tasted it (it is found in euphorbium, which is an old medicine), I'm just the first person to show/taste it on video. I'm not sure if they used taste to determine the Scoville rating for RTX though...they might have just applied it to mice (or humans) and monitored how little it took to get a TRPV1 reaction.

[@tomvolf]

@@LabCoatz_Science Ach, yes, of course, we do have Euphorbium officinarum as homeopathic remedy. So it is event current medicine, but in homeopathy potency. And I had to laugh ... you chemists are weird people. Yo (NileRed) says "is smells bad, but it is OK" and people around him are vomiting and fainting. And you can with nice smile eat the whole habanero (it would kill me instantly) and explain "it is hot, but just for short time period...". Anyway, thank you for explaining, how the Scoville rating is created, it was mystery for me.

[@1337fraggzb00N]

Next episode: blood of a Xenomorph.

[@The_OG_Soop]

This is probably not going to get read but I got some of this in my eye! I have a 30 foot euphorbia cactus in my front yard and I was cutting a piece up for my friend when some of the juice squirted in my eye! I was blind for a month while I was having dead epithelial tissue scraped off twice a week! Hurt like crazy this stuff is not to be messed with. I don’t remember if any got in my mouth but my eye definitely felt how spicy it is!

[@RoosterMontgomery]

I thought these cacti only occur in Africa.

[@Dragonclone]

That must have been pure agony😢

[@la24dogg21]

WHOAOHHHH

[@pbjandahighfive]

@@RoosterMontgomery They are native to Northern Africa, yeah, but that doesn't mean they can't be grown elsewhere.

[@elicarlson7682]

Wow

[@HakureiMikko]

Roughly twenty years ago, a few mates and I discovered the effects of euphorbias. By some weird coincidence, the huge spurge in the window sprung a slight leak and "someone", who may or may not have been myself, decided to taste the latex. I wouldn't call it spicy or pungent but definitely numbing, as in having a totally desensitized spot on my tongue for two hours. Now, seeing as large amounts of alcohol was involved, this quickly became a component in a punishment game to everyone's delight, and severely slurred speech. No lasting effects, but in retrospect quite stupid.

[@jasonrobbins4227]

It is stories like these that spawned the phrase "live without regret" 🤣🤣🤣🤣🤣🤣

[@outerrealm]

Ask any good chemist - alcohol = liquid stupidity. Same effect can be had by repeatedly hitting yourself in the head with a hammer.

[@outerrealm]

@@jasonrobbins4227 translation: live stupidly, repeating all your mistakes as well as making new ones.

[@curve5746]

Lol all men who make it to 30 are lucky

[@2degucitas]

Seeing how people have latex allergies using it as a numbing agent probably would be a bad idea

[@dw4223]

Nile Red is literally one of my favorite channels and I'm always on the look out for more great content in the same vein. You've got a new follower in me, so keep up the good work!

[@LabCoatz_Science]

I appreciate that! I should have a new video coming out in a few days covering an easy OTC route to acetic anhydride, if that sounds interesting to you!

[@megamaser]

@@LabCoatz_Science Yes that will be a very interesting video for those looking to synthesize heroin I mean aspirin in their basement.

[@stopthephilosophicalzombie9017]

@@LabCoatz_Science I think you should do a peyote extraction after that. : )

[@chypearce8457]

Same. I literally subscribed as soon as he name-dropped

[@RaptureDragon1]

Check out "I did a thing" on KZbin if you haven't seen him before =D you might enjoy too.

[@ElementalMaker]

Holy smokes man you are brave! I remember going down a rabbit hole researching rtx, and was pretty terrified from my reading. You just smeared that toothpick around your tongue like a madman! Truly impressive isolation and testing. Glad it didn't melt your tongue away. Subbed!

[@LabCoatz_Science]

Thanks man, glad you liked the video! I honestly kinda wish is was a bit more intense, but I think it turned out well in the end. Next up for me, synthesizing thioacetone: the most putrid substance on Earth. We'll see if that triggers a more dramatic reaction from me, lol!

[@ElementalMaker]

@@LabCoatz_Science Looking forward to it!

[@au8363]

Jesus Is King.!!!

[@lowkeylysemith2568]

@@au8363 King of the fictional characters?

[@au8363]

@@lowkeylysemith2568 Jesus Is Very Real Deuteronomy 4:29 King James Bible But if from thence thou shalt seek the LORD thy God, thou shalt find him, if thou seek him with all thy heart and with all thy soul.

[@juggernautonfire]

Great video! Also a quick side note / potential further explanation as to why the isolated RTX fell short of the spiciness of the habanero: The capsaicin in chilis is dissolved in the oleoresin which acts as a carrier to distribute it to the taste receptors. NileRed Shorts featured a video of him trying pure capsaicin and the results were quite similar to your test of RTX. Dissolving the capsaicin in the solubilizer ethanol immediately unleashed its full potency. Maybe that's what was going on here as well

[@LabCoatz_Science]

Thanks! For the test, a small amount of ethanol was added (I think I actually mentioned that in one of the video text pop-ups).

[@2degucitas]

That might explain why sprinkling gochugaru on my baked spud did nothing. Probably needed to be added to hot oil.

[@TheToemeister]

I’d try dissolving the extract in vegetable or canola oil instead of ethanol for the taste test. I’ve read that capsaicin in oil tastes spicier than pure capsaicin due to the better solubility, allowing it to distribute across the tongue more easily. A lot of the ethanol probably evaporated off the toothpick while you were holding it too.

[@antozon5070]

Please keep in mind that the phorbol esters (to which RTX is related) are well known tumor promoters! Also, I strongly suspect that the vanillin stain is meant for TLC analysis. Given that your extract still contained other compounds it really is no surprise that vanillin/sulfuric acid turns black. But I do appreciate that you distinguished higher scoville rating and spicyness, something too many people gloss over. Also, kudos on the high production quality and cinematography, well done!

[@LabCoatz_Science]

Thanks, those are all excellent points! The stain test was meant for TLC, but I figured it would react the same in the vial as it would on a TLC plate, since both are just inert silica-based surfaces. I knew that the stain turning black wasn't a definite sign, but I figured I would still include it as a cool visual with at least some merit. Regarding the phorbol esters, I knew they would be present during the tasting, but I (questionably) rationalized doing the test anyways. In my caveman brain, the cancer risk from tasting a single drop of it was probably less than the risk from smoking a pack of cigarettes (something some people do daily). Not the best decision, but I'm hopeful that things will turn out alright in the end.

[@antozon5070]

@@LabCoatz_Science Oh, it'll react the same in the vial to the stain, of course. But you can't really say if it's because of RTX or because another compound or because of general tar formation. Vanillin/sulfuric acid is a fairly general stain for organics and if you get a mixture of all colors that will also be black. On a TLC plate you have the compounds already (mostly) separated from each other. Regarding consumption, it's going to be fine, just don't actually make a hot sauce out it lol

[@user255]

@@LabCoatz_Science Yes, they are not that carcinogenic. Many plants have them and the typical response is just rash. Chronic exposure is another deal.

[@au8363]

Jesus Is King.!!!

[@marshallbowdrie8562]

@@au8363no he isn’t

[@Finnnicus]

I’ve never seen someone do a diy column on chemistry KZbin. Congrats! Yours was thoroughly insane using ground up kitty litter, so I’m glad it was the first I’ve seen.

[@OmegaGrunt]

That's the thing about the scoville scale that I don't think a lot of people understand, something can be noticeably hotter at a lower number, its just easier to dilute. It's not really a measure of how spicy something is but how potent it can be at smaller doses. A good example would be on hot ones, people go crazy over 'da bomb' at 100k but handle the several million last dab fairly well.

[@TheDaringPastry1313]

Yeah, that's because da bomb lies since it has real pepper extract in it and is more like 1.1M on the scale O_O

[@BixbyConsequence]

@@TheDaringPastry1313 Yeah you can really taste the capsaicin resin in it. And nothing else, unfortunately. It has no redeeming flavors at all, which is why it seems so diabolical.

[@travistucker7317]

The show with hot questions and even hotter wings?

[@NothingValidHere]

They handle it better because they've fried their taste buds, and btw.... capsaicin isn't spicy, it's just the agent needed for the receptors in the body to have a reaction known as pain.... do your homework people.

[@AlexandreG]

​@@NothingValidHeredamn, this guy really knows his stuff, I bet you 95% of the guys clicking this video SURELY didn't know that already, so take the free lesson 😎

[@unslept_em]

scientists have a long and storied history of going "this seems interesting. maybe i will taste it" and this is certainly one of those moments lol

[@pyrojack8230]

Man was so disappointed with spicy foods he went out of his way to make Raytraced Spice. Absolute legend.

[@k-aw-teksleepysageuni8181]

I recently went down the "what';s the spiciest thing?" rabbit hole and found out about RTX. It is an amazing chemical that could possibly help people who suffer from chronic pain like myself. The fact it takes longer to act on pain receptors could make it a good candidate to study ways to make medications that target muscle and tissue flare ups. I get terrible neck, back, and rib/sternum pain so something like that would be a new lease on living.

[@AldoSchmedack]

As someone on morphine for pain and disabled for years I would be very interested. Has to be a better med for pain.

[@k-aw-teksleepysageuni8181]

@@AldoSchmedack It's terrible. I had to quit all opiates years back and rely on cannabis derived medicine. Often oil concentrate orally and vapourized for quick relief. CBD, THC, and the terpenes in cannabis are decent for pain, but they just keep me alive and without much complaint. The only problem is it's not completely numbing like opiates are. It's not like I can go out and lift things for more than 30 minutes without hitting 7s to 10s on the pain scale.

[@jetsh1ftman746]

....... Trade pain for pain🤔. Sounds self destructive

[@k-aw-teksleepysageuni8181]

@@jetsh1ftman746 You don't understand pharmaceuticals, clearly. This substance could be used to treat nerve receptors and "numb" them out of over-firing. They've started studying it recently, and it's clearly got potential.

[@jetsh1ftman746]

@@k-aw-teksleepysageuni8181 you don't understand sarcasm clearly. This substance could be used to treat Karen syndrome and make you a better person in general.

[@valiantwarrior4517]

Very interesting about the comparison to capsaicin and how the Scoville scale works. I’ve noticed that the Scoville measurements of different substances doesn’t equate directly to how they affect those who ingest them.

[@Goazbox]

Just wanted to take the opportunity to mention your channel is way way waaaaaaay underrated with 19k subscribers. You deserve millions!

[@LabCoatz_Science]

Thanks man! My next video will either be synthesizing a superacid or smelling selenoacetone...hope those sound interesting!

[@Goazbox]

@@LabCoatz_Science both do. Keep it coming! Greetings from the Netherlands!

[@Sleepyken900]

Honestly! These videos are legendary... you deserve millions of subs!

[@stopthephilosophicalzombie9017]

I love videos like this that ask a simple question and then go into detail why it has a complex answer. You make great educational videos which I'm sure will inspire many a future chemist and/or scientist.

[@HistoricallyAccurate247]

Very interesting video ! I can't wait to see your new sstc ! I built your world's easiest sstc and I run it on 6 amps .The results were impressive!I subscribed:)

[@DanniDuck]

Seeing you like all the comments, even new ones, is worthy of a sub. Good work

[@MrApokalipse666]

If you want to use cat-litter-silicagel for chromatography I would recomend to dissolve it in aqueous NaOH and precipitate/ wash it with HCl for a few times bevore using it, so you will get much more surface area and a better separation.

[@LabCoatz_Science]

I would have, but I wasn't in the mood to waste use up all my lye and HCl. If I was to do it again, I would do it with a proper gradient elution (pure petroleum ether to pure methanol). After, of course, I washed the silica with methanol to get rid of that (albeit inert) blue dye. I tried using acetone, water, and even gasoline to get rid of it, but to no avail. Only once I started the methanol elution did I discover what it is soluble in, lol.

[@ginger-edits9690]

Ruthenium Red blocks the nociceptors from perceiving pain which is concluded with peppers from an article that I read

[@daniel-wood]

I like how you just casually mention distilling gasoline to get the petroleum ether

[@neayt9952]

This is a really spicy video

[@boscorner]

I heard about this way back on vsauce and how it was used in trials to stop pain in terminally I'll dogs . Very interesting chemical

[@Johnfromthedesert]

Congrats, I think this video just got bumped up by the KZbin algorithm. Got a new sub here, you madlad. Keep up the good work... "responsibly." 😉👍

[@ffwast]

My entire digestive tract assures you the heat of a habanero does not go away quickly.

[@_AwesomeRabbit10000-]

12:21 You were so brave tasting that amount of scoville. 16,000,000,000 scoville! Wow! I do not want to eat that!

[@davidmcdonald7775]

Great video - thanks very much for your contribution to science. I can't believe it took me this long to discover you, but that's me now subscribed. I look forward to flicking through more of your vids! :)

[@acb9896]

It's the seeds. The pain is in the seeds.

[@JWoodcock]

12:20 - "So one of the first things you notice whenever you're tasting resiniferatoxin... " I agree, I noticed that during my last session 🤣

[@modolief]

Dude, you are the coolest ... I want to be a bad ass lab tech like _this_ when I grow up!

[@chikkenbonz]

Awesome video! This was very interesting. I thought pure cap was the pinnacle of pepper pain! Watch, in another 10 years pepper growers will figure out a way to grow RTX peppers... But yeah your channel ----> instant sub. Gotta see more man! Love the science & experiments!

[@Jseee]

Dude looks so stoned at 9:50 lmaooo

[@LabCoatz_Science]

Now that I look at myself, I can totally see that, lol. But nah, I was just very very sleep deprived.

[@tylernethery409]

First video i've seen from you.. late to the party but im glad to see Nile have some competition. I love this type of science and im glad to see others providing content as quality as this! wish you the best and ill be wathing your other videos!

[@djdrack4681]

Hmm. So you saw my comment on a previous video about trying this? Super excited to see the attempt before trying it myself. I find it to be one of those molecules I'm always surprised nobody talks about despite how utterly intense RTX is:

[@LabCoatz_Science]

I might've seen your suggestion, but I don't recall, lol. You might actually be more surprised at how NOT intense RTX is! You'll see it in the video, but RTX is possibly less "spicy" than capsaicin, despite being higher on the Scoville scale. The large molecular size stops it from penetrating nerve receptors very fast, and it's so overpowered that it tends to desensitize rather than excite like capsaicin. It's still spicy though, but in a very unique way...you'll just have to see once the video airs! Side-note though, I'm unsure of the purity of my sample, so I can't say that my reaction truly captures what pure resiniferatoxin is capable of. Based on my research, it should be less than 10% RTX by weight, since I failed to fully separate the other diterpene esters with chromatography. Still, there was enough to taste, which attests to it's high position on the Scoville scale!

[@djdrack4681]

@@LabCoatz_Science Brutal. I was hoping for it to be used as the deterrent in 37mm smokes used to deter the various trespassing bigfoot on the property. :_(

[@THYZOID]

Great video! I tasted the white stuff of the same cactus once but it wasn´t really spicy. Unfortunately it died so no large scale extraction on my part. You didn a great job anyways so I don´t have to extract it anymore. Hope the phenylacetylrinvanil tastes spicier because of higher solubility. I´ll add the part were you tasted this stuff to my phenylacetylrinvanil video if you are cool with it.

[@LabCoatz_Science]

Glad you liked the video! Good luck with your phenylacetylrinvanil project, and yes, you can definitely use parts from my video in it if you want.

[@THYZOID]

@@LabCoatz_Science thanks man!

[@coopergates9680]

@@THYZOID Is phenylacetylrinvanil also toxic, despite not having 'toxin', or could one safely make a salsa or sauce that has it?

[@THYZOID]

@@coopergates9680 Given the lack of information on it just assume it is very toxic

[@NeoMarv]

Nice video. But as a chemist I see many problems with that isolation. Resiniferatoxin contains many labile functional groups. You have an alpha beta unsaturated ketone, an ester and three epoxides which are connected by a single carbon and one of the epoxides is even an allylic epoxide which easily opens up when even seeing a nucleophile. Heating that molecule in water/MeOH with all the other compounds in the crude mixture most probably leads to decomposition. I am interested what the pH was in the heated mixture... If it's even slightly acidic or basic it's even worse. So you might not have tasted the actual resiniferatoxin but a derivative of it. Also when you say that the product was confirmed by a colour reagent, then that's just an assumption. Using the Rf value of a TLC on top of it would have been better but still no complete verification. I don't try to be negative but simply put I love organic chemistry and I do try to add information whenever ppssible.

[@LabCoatz_Science]

I totally agree with your assessment. The pH was fairly neutral, iirc, but I do think the prolonged heating probably caused a lot of unwanted decomposition. I do believe there was enough RTX to taste though, since I did try applying the raw latex to my tongue and I got the same effect (albeit somewhat weaker). That kinda leads me to another point: the plant was dying when I did the synthesis, and it didn't seem to have as much RTX as it theoretically should have. I've heard stories about these plants giving debilitating burns and discomfort just by chopping them up, and this one produced no such effect.

[@NeoMarv]

@@LabCoatz_Science I agree with you and the potential derivatives might also have a similar effect though. It would actually be nice to find out which compounds were in your final product but that would be quite costly and time consuming. I think this was a nice project. I did not know about Resiniferatoxin before and that's what's so good about such videos, you learn new things in an entertaining way!

[@jonadams8841]

30 years ago, when I bought a house in the San Gabriel foothills north of LA, I was told that the incredible assortment of euphorbia cacti all around the house and property had been collected as cuttings, by the previous owners in their global adventures. There were dozens of types, from low shrubs to tall tree-ish things. And thorns and spines. Some had metal tags that gave the formal name. Again, I was told that the white sap/resin/latex that would ooze forth when the plant was damaged would burn the skin, and if inhaled, or exposed to the eye, would necessitate medical treatment. The Internet was not quite a thing, but the LA County Arboretum was just down the road. There, they had many of the same plants, but I could find no one who could really tell me much about the plants, except for the pain aspect. In the decade I had that house, I never experimented with the sap. Now I wish I did 😂

[@jkfury.x1254]

11:42 you could see the fear in his eyes

[@LabCoatz_Science]

Pfff, nah! That was honestly more sleep deprivation than anything, lol. Living with five other people (and pets) means I have to stay up pretty late to get shots like this done without background noise.

[@dddd6606]

"RTX given intraocularly has an unexpectedly poor potency to provoke pain response" is one of more chilling sentences I've seen in scientific paper.

[@stupidburp]

Probably done for pepper spray product research

[@septixskeptix1107]

I tend to not eat compounds that end in the word "toxin." I guess I just have some kind of weird hang up with that word. 🤷

[@narkedandafraid]

Glad to see your channel growing! You’ve got talent.

[@theantiorthodoxclothingcom4152]

Great video! I was wondering if anyone was insane enough to eat this

[@ste887]

quick tip, if you need vanillin powder later on, most stores that sell DIY vape flavours offer pure vanillin powder at a couple dollars gram for pharmaceutical purity, least, they do in the uk. with the vape laws in America things might be different

[@Sparkette]

This video sponsored by NVIDIA

[@Ori-lp2fm]

Underaged comment

[@Ori-lp2fm]

Underated

[@AlexandreG]

That's deep bro

[@SomeCanine]

It's kinda similar to how acids can be technically stronger but also less corrosive. Sometimes things just don't work the way you think they should.

[@Nefville]

OMG I have this cactus. I've had it for 20 years and I grow Carolina Reapers, the new variety. Have my first Scotch Bonnets overwintering... Okay yeah this is way out of my ability to make. Still its really cool that of all the plants I've had, this one I've had the longest, since I was a kid and all this time I had the spiciest substance on Earth while at the same time I became a huge spicy pepper fan and cultivator. Great video, unexpected results. It makes sense though.

[@intensecutn]

Fun fact: Euphorbia are not a cactus. They are a succulent.

[@Nefville]

@@intensecutn I think I am wrong, after the video I was looking at other pictures of Euphorbia and they look like they bloom which mine has never done. Also its a fairly obscure plant from Morocco and I'm not sure that's something I would have gotten by accident 27ish years ago. It looks damn close though. I'm not a chemist so I'll have to stick with my Reapers and Scotch Bonnets for now but I did find a reasonable source for resiniferatoxin online.

[@Cineenvenordquist]

@@Nefville Good luck getting that cactus or other succulent to bloom and surviving a resin tap from it if you're drawn to make forbidden negrito from it.

[@thisismyusername6717]

You can just lick the catcus when you cut a piece lol

[@jonnylawless6797]

Man I want some of them scotch bonnets for jerk

[@davida1hiwaaynet]

Fascinating! I have always enjoyed habañero peppers with my Mexican and West Indian food. Didn't know there was a (supposedly) more potent active ingredient than capsaicin. Thanks for showing this and tasting it!

[@totalmetaljacket789]

The tilde in habanero is actually a common misspelling.

[@sillymizu]

WHY IS THIS SO UNDERRATED

[@au_gmentedreality]

Good job dude! I enjoyed the breakdown science. And great delivery too! Viva science

[@SaltNBattery]

I had a panic attack from trying Carolina Reaper... I couldn't talk myself out of it, thought I was having an anaphylactic reaction or something. Pretty embarrassing but I have Panic Disorder, so it doesn't take much.

[@LabCoatz_Science]

Me too, to a lesser degree, although I'm getting better with it now!

[@jerseylife8701]

I could definitely see a reaper causing a panic attack lol.

[@EloNaj]

@@jerseylife8701 Yes I grew reaper but I would never try to eat them raw. Never ever XD.

[@Docosi]

You deserve more subscribers. So much improvisation and ingenuity.

[@morestupidforms]

Water is the last thing you want with spices, it closes the taste buds and traps the spices in. You want lots of milk.

[@legitimatehuman1220]

The scoville rating for a pepper is for the FLESH of the pepper, not the whole pepper. The colorful outer shell. The seeds and the pith t hat attaches the seeds to the flesh is generally much hotter.

[@Piocoto123]

Pretty cool. I am really into cacti and euphorbias and have tried a coiuple of their latex directly. Tried Euphorbia aeuruginosa.. mehh, tried euphorbia parciramulosa.. holy hell, the heat was there for about an hour and was as bad as a very hot sauce. I have an E. resinifera but after trying the other one I dont think I need to try that one ever

[@LabCoatz_Science]

Honestly, I tasted the latex directly, and it wasn't bad. I'm beginning to think my euphorbia was on the more mild side, since it didn't even irritate my skin or make my eyes water (although it was spicy). Not sure what is in the euphorbia parciramulosa latex though, but if it lasted for about an hour, it might've been resiniferatoxin, tinyatoxin, or a similar "super-capsaicinoid".

[@Ulvens]

First time here. Nice concept, and video. Glad to see someone doing full format chemistry again. Nile mostly does shorts nowadays, and I'm not into it.

[@jaakkooksa5374]

At 0:37 he states that "no record exists of a human tasting this substance". So, how do we know that it is extremely spicy?

[@LabCoatz_Science]

I was talking more about the pure substance, since people have tasted the latex of this plant before and found it to be hot. But scientists determined that this compound would be "spicy" based on the neurological reactions of laboratory mice, which indicated very high TRPV1 action (the heat receptors also triggered by capsaicin in spicy food).

[@seanmills7255]

Earned a sub from me. I love science and love how much information you gave during the experiment

[@upsignerman]

The therapeutic applications of RTX in that one paper screenshot were so interesting! Non-toxic topical analgesic for painful burns? Woah

[@LabCoatz_Science]

Yep, and I can personally confirm that this stuff works! One experiment I tried later on after I edited this video was applying RTX to my tongue and then eating the habanero again. Apparently, everything people say about this stuff deactivating heat receptors is right! I felt NOTHING from a +400,000 Scoville habanero pepper!

[@eruiluvatar236]

@@LabCoatz_Science Did the sensitivity to capsaicin come back after a while? I remember reading that it can kill the nerves responsible for pain/heat but maybe it was an exageration/just the receptors that regrow after a while or it just happens at larger doses.

[@LabCoatz_Science]

@@eruiluvatar236 With my sample, the effects wore off after roughly an hour. As of now, I can taste spiciness just the same as ever, so if RTX does kill nerves, it must need to be fairly concentrated!

[@eruiluvatar236]

@@LabCoatz_Science Thanks for the answer. It could make for quite the party trick then. Not really suggesting anyone to try but I see someone having a tiny bit of RTX and a short time later chugging a bottle of some crazy hot sauce like if it was water (and maybe going to the ER because of the rest of the GI track likely won't be too happy).

[@actoryz2049]

This video is underrated

[@blbmnm5939]

u're not normal :D, stumbled upon ur channel via the thioacetone video done by that nilered guy or smth, but u are just next level

[@spynae]

God I love video lab reports, you've got yourself a new subscriber

[@MyDixieWrecked247]

1:25 The guy who made the Carolina Reaper bred an even hotter pepper called Pepper X. Nearly 3.2 million SHU, almost twice as hot as a Reaper.

[@establisha]

loved the longer coontent, keep it up bro!

[@snarevox]

i wanted to know what part of the tongue contained the "spicy" taste buds.. however, after asking google, i learned that spicy isnt a taste, its a pain response generated by the spicy chemical from the spicy food making contact with the nociceptors after being dissolved by saliva.

[@KratsminschSkunk1]

I had two skunks who liked habaneros as much as I did. Agent Cooper, and Professor Moriarty. I had to record and post a video of them eating one. I wouldn't try what you did, but it's been intriguing enough I watched you through the whole process.

[@SaltNBattery]

Really cool video. Your channel deserves more subscribers.

[@z_monty]

Very informative, subbed!

[@idontwantahandlethough]

Bro that little intro was hilarious! Kinda reminded me of something you'd see back on Spike TV (FOR MEN!!! 💪😤cringiest branding of all time lol) Your show would have been called _Dude Science!_ or something.

[@WilliamKKidd]

Are we just going to gloss over you nibbling at a habanero pepper?! and then being like "Yeah, it went away in 30 seconds"?! Bro, you are a beast... godspeed!

[@randomdudeface]

Yo this was really interesting! Also I *LOVE* spicy stuff, so... 🤔 I should make a hot sauce out of this stuff sometime...😁

[@Kuli24000]

Ah, just as I would have done... with the uh... di-aquius phase thing...

[@chomp_5412]

Great vid!

[@garymillwacker8486]

LMAO!!! your face when eating hot peppers is about as dramatic as someone drumming their fingers on a desk during a rainy afternoon.

[@CaptainCanuck74]

"...given intraocularly..." In the eyeball? Please explain! Also, why not taste a 1:1000 dilution of RTX to test your theory that it's still detectable?

[@LabCoatz_Science]

I honestly have no idea what they did, but I feel bad for whatever creature got this in its eye! And come to think of it, I probably should have tried a dilution to see how strong this stuff really is, but my sample wasn't a very potent one, so it probably would have been underwhelming.

[@CaptainCanuck74]

@LabCoatz_Science I imagine that your final conclusions may have been different if you'd jammed it into your eyeballs! Thanks for the video, great job

[@hoggif]

Distilling with graham condenser horizontally? I wonder how much a problem will all the liquid in the bottom sides of the spiral be. Graham is used vertically to avoid trapping any liquid. This can also result in more risk of bumping when the condenser is "blocked" by liquid in repeats of blowing it out. For getting the solvent away this can work but for separation a real no-no. If you have, use something other (but not the "bulbed" Alihn that also traps liquid). I guess liebig is the most used for distilling and those are dirt cheap too due to easy manufacturing. They don't trap liquid when slightly angled from horizontal.

[@LabCoatz_Science]

I actually set up a fundraiser recently to help me afford a Liebig condenser, since so many people have said the same thing, haha! Sadly, I currently only have an Allihn and a Graham condenser, and none of the glassware needed to set the Graham condenser up properly. Hopefully that'll change by next year!

[@hoggif]

@@LabCoatz_Science Hope you can fund it. Setting up it properly vertically is usually a hassle and works only with a column at least as tall as the condenser. Sometimes alihn can be better as many of them work better when angled partially (think of a tilted column to get idea what I mean about tilting it more than normal). On some condensers bulbs have so steep angle this may not work though. Grahams are a pain for refluxing too as the vapor tends to push droplets upwards. I've never used my graham but when extreme cooling is needed (and only when) it is worth the hassle I think.

[@romanmir01]

something has 'toxin' in its very name and you still put it into your mouth, I applaud the effort)

[@The144Kth]

There is another very interesting feature of that plant, that I wish some scientist would investigate. It is this: If one of the thorns gets under your skin, it will sit there, and no amount of pinching, poking or prodding will cause you any pain or discomfort. Even if it's close enough to a bone to be pressed into it, you will feel nothing. I had one in my forearm for several month before I finally said, enough is enough and dug it out.

[@ClaptimusPrime]

When the police come to your house with pepper spray, you fight back with this stuff

[@berylman]

Great chem video! I kind of wish you had done more rigorous purification and confirmed the molecular identity by other means

[@h.Freeman]

We had one of those cactus' and my dad got some of the sap in his eye and he screamed like a mad man...at least i think it was that cactus...could have been Peruvian torch tho🤷🏿‍♂️ but it probably was that one...he didnt hallucinate

[@Howtoeatrocks]

I just had a flashback of cutting up cacti in my grandmothers back yard for a “potion” and the resin getting in my eye. I had hoped to never remember

[@stickinthemud23]

I want to know how a teeny drop of capsaicin oil and disburse among a quart of spaghetti sauce in order to "hotten up" the whole batch.

[@strangehappenings8961]

This guy gives some real David Hahn vibes. Haha cool video man. 📹

[@fazergazer]

Immediately subscribed and look forward to seeing your work🎉

[@vinnycochrane5139]

I went to a friend’s birthday party at a Hungarian restaurant in Soho. My friend warned me not to touch the tiny peppers in a bowl on the table. Of course I had to play the tough-guy, which ended in me thrashing on the table, shrieking for the intercession of a saint, or even the release of mortality.

[@ADHDWOOHOO]

Man the pure DIY energy here is awesome. I've had pure capsaicin before and the sensation was kind of similar to yours. I then drank a beer and my life was instantly ruined. I wonder if you dissolved the RTX in ethanol it would hit harder.

[@pvanukoff]

Regardless of how impure the RTX was, this man has giant brass balls. Respect.

[@JDogggg69]

You should try the latex as is. It would be interesting if the stickiness enhances the spiciness, or if the concentration is too low.

[@LabCoatz_Science]

I did it wasn't much different (although it was a fair bit weaker).

[@NootalieWalf]

What’re the odds I hear about this stuff randomly 5 minutes ago and there’s a long video about it posted 3 days ago… neat

[@LabCoatz_Science]

The world works in weird ways sometimes! Glad you found the video, hopefully I was able to answer any of the questions you might have had.

[@syncroslicer]

Great video dude! Super interesting!!!

[@coopergates9680]

Major props for this one. Is there really only one source (namely a KZbin video) showing people taste testing different salt compounds, like KCl, NH4Cl, SrCl2, LiCl, etc.? Even if you don't do a "most salty substance ever", which ones taste decent enough? Apparently calcium and potassium chlorides taste awful, and rubidium and cesium not too good, either.

[@sdfkjgh]

@coopergates9680: Now I wanna taste ununennium chloride. #CanYouLickTheScience

[@AngryK1tty]

Been waiting to see some videos on this stuff

[@allenhonaker4107]

Very Rod Sterling ish intro. I love it♥️

[@technodrone313]

you build up a tolerance to spice. after growing a ghost pepper plant and making sauce i barely flinch on habaneros anymore. ghost peppers are so good :)

[@LabCoatz_Science]

Can agree, I grew ghosts and reapers this last year and made a sauce from them, and I love it on everything, haha!

[@alphonsereitz]

I'm probably background noise.. I love these chemistry, I think I'll like yours as much as Niles. I'm sure it wouldn't be popular, but if you use stoichiometry, i think some sould be shown. Either way.. really cool video

[@LabCoatz_Science]

Nobody is background noise in my comments section! I do try to include the stochiometric chemical balances whenever possible, although the main focus is typically getting to the end product, haha! Glad you liked the video, I should have another coming out in a day or so!

[@imstupid880]

Gotta love how budget chemistry is basically just modern day alchemy.

[@imetzl9340]

Eating the Habanero first also loaded up your capsaicin receptors, making the next intake of capsaicin much less noticeable. Could also be a contributor

[@bud8254]

Awesome video! You earned yourself a new sub my friend.

[@anthonyrickardii6198]

Definitely indeed interesting and awesome video. I wonder what would happen if you used it as a mace?

[@LabCoatz_Science]

Honestly, it might be about the same as capsaicin. According to the reports I read, the powdered latex from the resin spurge was apparently used in "ye olde days" by pranksters to make people sneeze and cough, so its use as a Mace-type irritant isn't out of the question!

[@anthonyrickardii6198]

@@LabCoatz_Science that’s indeed interesting. Thank you