Learn About Illumina's Infinium Protocol

[@Igcaet]

I have a question: if we have a CG heterozygous (like I do have in my data), how will the scan differentiate both green signals and not misinterpret it as being homozygous? The illumina reference guide itself does now cite "fluorophore", not even once. And both there and in their videos, they only show biotin associated with C and G, which after will have a green signal, and DNP with A and T, with a red signal. How is it possible to differentiate CG or AT heterozygous?

[@Igcaet]

OMG, this video is perfect! I've searched it for so long! Thanks for uploading, even if it was only in dec 20th, better than never xD

[@HewanDemissie]

Thank you. This is excellent. Thus, I can work in the lab on my sample.

[@jrodriguez9968]

You missed the XC4 covering and what about the heterozygot combinations of C,G Is the same as G,G and C,C? and T,A is the same as T,T and A,A?

[@yohanaalemseged6683]

thank you for this very intersting !!!

[@ДмитрийНовиков-е8ж]

Hello! Im glad to see this video! And i need a little help. What is the maximum time period between chip dying and scanning? Can i dye chip in my laboratory and then read in second laboratory the next day? Thank you!

[@Igcaet]

Helo! Here with my colleagues we used the Infinium Multi-Ethnic Global-8 Array, and it took us three days the whole process. In the third day we've stained our chip, then after washing it we've put on a vacuum incubator and after 1-2 hours it was completely dry, so we could scan it, with excellent call rates. Watch out for the chip must be read until 72h after staining.

[@ДмитрийНовиков-е8ж]

@@IgcaetThank you for answer!

[@jrodriguez9968]

Yes you can but cover it from light

[@user-om2xt6bs8d]

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