DNA Microarray Methodology

This animation demonstrates how DNA microarray experiments are performed. One common use of microarrays is to determine which genes are activated and which are repressed when two populations of cells are compared. Every gene is measured simultaneously. As an example, we’ll compare what happens to yeast genes when cells are grown in aerobic versus anaerobic conditions.
Written by:
A. Malcolm Campbell, Ph.D.
Copyright © 2017 NC Community Colleges and BioNetwork

[@GrandeNutty]

This is beautiful. I swear, my prof made this sound like the most complicated thing in the world.

[@acalp]

Best explanation of microarray on KZbin

[@NCBioNetwork]

Thanks for the feedback!

[@EpicSelenium34]

Excellent explanation and pristine graphics. This video really helped me comprehend this difficult topic. Thank you!

[@joshfernandez6089]

Shut ur mouth

[@bean_odinson]

@@joshfernandez6089 you didn't like the video?

[@jujubajtn]

Thank you for this video!! You helped a undergraduated student of Biotechnology here.

[@drewscampfire]

This video is top-notch! What a great explainer video! It really helped me understand the nitty-gritty of DNA microarrays. Thank you so much!

[@englishiseasy6092]

I have seen to many videos about microarray but I can say surely this is the best. Brief and useful. Thanks 👍👍👍

[@taladiv3415]

Thank you for saving me precious time and mitigating the frustration involved in boresome reading of an unfamiliar topic for the first time. Now I am primed to understand the dry text.

[@khizirabbas9226]

A very useful and easily understandable animation to know about the methodology of Microarray. Thanks a lot and stay blessed. Regards.

[@YasmineNazmy]

This is the best scientific explanation video I have ver come across. THANK YOU!

[@coolkitty154]

Very clear and informative, thank you!

[@qp5337]

Really great explanation, the visuals really helped. Thanks

[@rogsang9764]

excellent video, the graphics are trully helpful, thanks for share this valuable information

[@ananyapaul9148]

this animation was too good. best way to explain 👏👏👏👏👏👏

[@makiiu]

best explanation to understand microarrays, thanks

[@silentcloud6342]

this video is the best microarray explaination.

[@Kh471D]

best. medical. animation. iv'e. ever .seen.

[@dinaaly2082]

great video

[@msmemoona7198]

Excellent presentation

[@TheNaiaratarifa]

Hi folks! excellent animation, thanks! But can you enable the CC subtitle option that allows others to create subtitles? I really want to translate it to Portuguese. Thanks!

[@NCBioNetwork]

Added! Sorry for the delay.

[@joaogoncalves5480]

Remarkable work and explanation!

[@dinatjahanmilitasnim4614]

Thanks a lot

[@Forensicforumexpert]

very nice explanation!

[@ankitachowdhury5268]

Very nice explanation mam...plz upload more videos like these...it's really helpful...

[@sophianotopoulou262]

the best explanation!!!

[@faisalbinrahman9722]

it was very informative. thank you very much. :)

[@kerolloskamelsaeed5615]

Wow that was helpful and also entertaining.. thank you so much

[@afreenfatima1004]

really good video.. but i have a question.. when we add cDNA (probe) on DNA chip having 6000 genes of yeast then how this probe will bind to that DNA present in gene sequence? bcz DNA is a double stranded molecule

[@rasheedolajiire5135]

The single stranded DNA copies are made from the genes and can easily hybridize with the probes (which are also single stranded DNA)

[@ruhidyusifov5106]

Thank you so much

[@NCBioNetwork]

You're most welcome

[@mulhmzuhili]

Yep this is best one.

[@tarunkadian1178]

Thanks a lot ...superb explanation😊

[@noorulain6066]

Amazing , excellent explanation ❤👏👏❤

[@devanshuradia3466]

thank u so much.. great explanation

[@hamzashaikh3122]

which software you use for animation

[@pratyush136]

excellent

[@mohammadshormij315]

very good thank you

[@itsrooh645]

loved it

[@mihaleo3216]

Excelent grafics

[@paulahrens6362]

could you do videos on other analysis like w-blot or ELISA ? Anyone here with good recommendations?

[@zeelpatel8049]

thank you

[@DanielTerceiro9]

very good!! thank you very much!

[@NCBioNetwork]

Thanks for the feedback. We are glad the animation is helpful to you.

[@gauthampaul4213]

what apparatus is used to wash off the unbound cDNA?

[@Nooneonearth2.0]

is it possible to model the DNA microarray in microfluidics?

[@ajibara9970]

AMAZING

[@AhmedHamed-ot1ez]

Perfect Thanks

[@brendasolangeayzanoacanale7831]

awesome!

[@mylordandsaviour4786]

1:02 Did they change the tip?

[@marsolata]

of course they should do that

[@akhil.nitrkl.22]

Wow🔥

[@axeltrujillo5693]

SCIENCE!!

[@user-gm6ek6dc2p]

1:35 but these are red and yellow lol

[@shree9728]

How's the mRNA is converted into red or green cDNA?

[@frangimenez4674]

A reverse transcriptase is applied to the mRNA, which makes the cDNA. Then, this cDNA is marked usually with a fluorescent compund. That fluorescence is what the scanner analyzes and allows to differentiate the different cDNA strands

[@shree9728]

@@frangimenez4674 thank you Sir...

[@hanchung8237]

For my final.....

[@student_remo]

❤

[@shabinabano275]

👌👌👌

[@Shakeel-]

👌

[@JYOtiRaNJanMANgaRaj]

🙏🙏👌👌👌👌

[@BeeRich33]

Still seems rather manually intensive. Ideally automated much like bloody chemistry analysis.

[@somerandomperson3970]

Yeah, Gen-Z will be a bunch of geniuses

[@056vatsalapandey3]

PPEERRFFEECCTTTTTTTT