[LIVE] Making Yeast produce Deer Milk and Egg Whites - Whose Gene is it Anyway #1

I'll be picking a random and ridiculous idea out of a hat, and then researching and designing a DNA construct to accomplish it, LIVE!
For this first stream the idea has been prechosen "growing milk and eggs without animals" but future streams will be random.
Finished plasmids: github.com/thethoughtemporium...
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Support the show and future projects:
Patreon: / thethoughtemporium
Ko-Fi: ko-fi.com/thoughtemporium
Become a member: / @thethoughtemporium
Store: teespring.com/stores/the-thou...

[@balahaasdf2219]

Stream starts here: 1:48

[@doctorpurple5173]

Thanks

[@atomspalter2090]

thx

[@thisismambonumber5]

og in the comments

[@ayushpaul6863]

ty

[@christopherwillows5515]

Good man

[@haileythurston6280]

I really appreciate how much emphasis you put on the fact that gene editing is difficult, time consuming, and doesn't always work out even when you know it should be right. I'm only an undergrad and I've been in a lab that does cloning for a little under a year. I get easily frustrated with myself when cloning experiments fail, even though I'm super new to this and have a LOT to learn. It's nice to hear that failure is just the only way to improve when dealing with genes. The thought helps me find patience. :)

[@SoulDelSol]

It's fascinating to me as someone who grew up with this not being possible to hear undergrads doing it and getting frustrated when it doesn't always work

[@yoboirelly]

Mmm affair Ooooo

[@Nooneonearth2.0]

Man, They didn't include giraffe and Beta casein of giraffe is 89% similar to bovine. Try out!!

[@Goodengelt]

And it's a kosher species, so that's nice. Endangered top.

[@biglez9033]

glad I could help you out there, seeing that EVERYONE who watches you can contribute to this is amazing! I'm working as a biotechnical assistant (basically working hands-on in a Lab) and really enjoy your content. Me and my classmates always joked about creating weird vectors and making them real, i guess you took it a step ahead and actually did it, which is awesome!

[@Ryan6.022]

This is awesome

[@piteoswaldo]

You've earned a lot of internet points here.

[@lucasg8201]

@@piteoswaldo ill

[@schlega2]

Now I kind of want to take the sequences from a bunch of different birds, reconstruct a common ancestor sequence, and make dinosaur egg whites.

[@connorfoley8114]

Why not just make an entire dinosaur?

[@schlega2]

@@connorfoley8114 at 18 cents per base pair? I don't have that kind of money.

[@connorfoley8114]

Schlega Gofundme?

[@MrTheWaterbear]

I’m doing this with a special class of diterpenoids from Australian succulents ^^ It’s such a fun project fusion of genetics, bioinformatics, synthetic biology and phytoengineering!

[@jadenpeterson4881]

I would love to see a yeast that produces its own alpha acids so hops are not required for flavoring beer.

[@mrmolasses5366]

Yeah I would like to see some yeasts for that

[@cdgonepotatoes4219]

I'd just love to see golden yeast for beer, as recently unfiltered beer found a bit of a resurgence why not make it multivitaminic.

[@IoFPV]

@@baumi8125 I saw that comment and I was like "it is already done"

[@Castle3179]

@@baumi8125 Yeah!

[@stdorn]

I agree with you F the company who tried to patent nature. However this is a strange time to do this while farmers are dumping milk down the drain.

[@dutchik5107]

You can't get it in stores tho. And it takes a while to get it made. This you would have at home

[@j_razavi]

I'm not sure, but I think that's to do with the cost of transporting milk (chilled lorries etc). At a society level, brewing it at home would be way more efficient.

[@zeuscivilian3010]

They forgot llama

[@SoulDelSol]

@@j_razavi human women should be able to keep brewing it at home. If they keep pulling it out the body keeps producing it

[@rustym.shackelford5546]

I never understood why people would patent nature. Really I agree w/ the dude in the video in that "owning an idea" is a foolish and greedy concept.

[@AngryK1tty]

I fell asleep and woke up to this mans voice. I went back to sleep and incorporated him into my dream. He was the villain.

[@maxantonweber]

Instant like for your opinion on patents

[@nagitokomaeda3237]

Especially software parents. They should never have existed.

[@TinyGiraffes]

Patents don't exist because you have a right to own ideas, they exist because they promote growth economically, the problem is that, like most things, Disney fucked it up, they had it extended and now everything will stay patented until at least 2024.

[@elliotn7578]

The goal behind patents is to give individuals and small businesses a way to compete against large corporations like Amazon and Google. Without patents these corporations would just copy your technology and use their massive amounts of money, connections, infrastructure, and other resources to instantly out-compete you. However, because of patents they must find alternative methods or workarounds if they wish to compete; this drives a significant amount of innovation in the space and results in the exploration of ideas that otherwise never would have been considered.

[@mortemmalum7228]

@@TinyGiraffes That's copyright that you are thinking of which is 75 years afterlife of the creator, a patent only lasts 20 years and always has. edit: grammar

[@grn1]

@@elliotn7578 That's the goal but what actually happens much of the time is the large companies either steel your patent and pay off the courts to make it theirs or force you to sell it to them. Sometimes they go on to make the product you envisioned, sometimes they just sit on it so they can continue selling an inferior product for more money, and sometimes they make the product but take shortcuts which turn your vision into garbage. If it was up to me things would be free for personal use or a 20% royalty if used for profit.

[@KarolOfGutovo]

Headlines in a month: "A youtuber distributes a virophage targeting coronavirus"

[@ginger_biscuit]

If only...

[@pafnutiytheartist]

Still waiting

[@digifomation]

the ovalbumin yeast, can you imagine the foaming this gonna produce, yeast produce CO2 during fermentation, that goopy mix gonna foam like crazy.

[@weirdal3333]

Take that air and bubble it through spirulina to cancel the carbon footprint

[@warro-jg2yq]

@@weirdal3333 wouldnt it be possible to combine the yeast mixture with some sort of algae?

[@Castle3179]

INSTANT MERINGUE! You're a genius!😯

[@gromit7573]

Some capitalist: you can't just side step this big corporation's pattent they need to profit from innovation ;m; Thought Emporium: haha deer milk yeast go brrrrr :)

[@guterflex7967]

I don't know whether I would call myself a capitalist but I believe in a free market and I still think that patents are dumb in some way. Because it's unnatural. It's rather something that's enforced by the state. I don't think that's capitalism. (Although I am not an expert; convince me otherwise)

[@Terszel]

@@guterflex7967 Free market is less about economy and more about confidence, no one wants to be apart of a free market if there is no confidence that you will benefit from it, its why the Fed is comfortable propping up the market atm, inflation is nothing compared to deflation, which can arise out of a lack of confidence and participation in the market, hope that helps justify why things like patents are needed

[@TheSugus38]

@@Terszel Exactly. Capitalism, for all it claims not to want a strong state, needs all kinds of regulations enforced by states to strive. It cannot, by nature, exist without the power of the state.

[@Terszel]

@@TheSugus38 The beauty of capitalism is that it requires very comfortable systems to get started as opposed to other forms of economy. A legal and fiscal system is all that is necessary and 99% of human beings are ok with those two

[@pauljones9150]

Brrrrrrrrrrrrrrr

[@Aaron-lr5gw]

Amazing watch this, him doing his smart stuff Chat doing, chat stuff

[@notapplicable7292]

bit of a shame he didn't make the milk red

[@recinbersvoice8802]

Very excited to see where this series goes and what I can learn from it.

[@cicada5072]

Recinber's Voice I agree, watch the entire thing last night

[@abdAlmajedSaleh]

i will watch just to give him views , i don't understand half this shit

[@420alfonzo6]

I like how he treats shopping for the right plasmid like trying to find the right vaccum on Amazon.

[@HorochovPL]

29:04 You probably missed opportunity - create platypus eggs and milk. It's a reference to platypus omelete meme. Update: 2:00:47 Okay, somebody suggested milk :D

[@dutchik5107]

There were many more possible animals. Deer was just more obvious

[@makeracistsafraidagain]

Your DNA molecule has the correct ten base pairs per 360 degrees of rotation. Almost Nobody gets that right. My Wife was one of the very first scientist to physically remove a nucleus and replace it. We're old and retired now.

[@DanielSMatthews]

I'd like your feedback! Vaccines for COVID-19 are a long way off and will not work for several groups of vulnerable people, that is why I proposed the engineered probiotic bioshield, it uses a receptor decoy to trap and destroy the viral particles and if the virus mutates to avoid the decoid you have also forced it to become unable to infect humans. Neat huh? The bioshield method also helps protects against ALL viruses that are transmitted via the mucosa. Mind blown yet? Another nice feature that is has is its dependence on a artificial amino acid/s, this means that you can deploy it and it will just die off, unable to survive in nature, then you deploy the next version which uses another amino acid, you cycle these one after another (3 or more) so that you have continuous protection but your solution cannot live long enough to be problematic and it is easier to roll out upgrades to its genetics. Later you can add additional genetic material to have the bioshield bacteria shed bacteriophages that also attack pathogenic bacteria too. What this means is that it eats pathogenic viruses, digests their components then uses the raw materials to produce other viruses that only attack "bad" bacteria all the while being unable to survive without humans supplying it with its essential artificial amino acids. None of this requires novel biotech that has not already been studied! That is the part that astounded me while I was researching the feasibility of the idea! But there is one problem, old people will not die as easily as they do now, so once cancer is conquered you are left with large number of very long lived people with dementia etc. Anyhow please take good look at the idea! --> dsmatthews.blogspot.com/2020/04/covid-19-solution-changing-rules-of.html

[@DanielSMatthews]

@@blackjoker2345 There is no profit to be made, this is about saving lives and is open source. Nothing is injected. It is a bioshield that can have regular genetic "software" upgrades. It is a programmable externalised immune system layer. The development costs of each new upgrade is potentially significant, but the rollout across the globe will be as cheap as sending inoculant for people to brew up locally at very low cost. The virus trap works on its own but you may as well have it recycle its viral victims into weapons against "bad" bacteria. However this phage feature is just a nice secondary feature, not required for COVID-19 or any other similar viral diseases.

[@DanielSMatthews]

@@blackjoker2345 LOL, it isn't a product it is a lifeform, it builds itself, with minimal help from humans once it is designed. That is one of its key features. A single liter of cell culture shipped to a country can scale up to national level coverage within weeks and being lactobacillus you can grow it on as many different substrates as there are existing fermented food types. i.e. It can grow on fruits and vegetable wastes from the food industries. Make it at home like you brew beer, or in the megaliters in a factory it works at any scale so in any nation. The only limiting factor is the exotic amino acid needed to control its ability to grow and that can be engineered to be minimised, just enough to get the job done and then without it the bacteria is living on borrowed time, according the the previously mentioned time-table. It is the Linux of artificial immune systems. Nobody will get rich off it, yet humanity will flourish because of it so the entire economy will boom and people will get rich of that.

[@DanielSMatthews]

@@blackjoker2345 Also NB open source Linux runs the entire internet. People don't sell Linux, they make wealth from what open source software enables. People just don't get how that works and make childish and simplistic comments about various political systems, but the proof is in the vast complexity and wealth that grew out of the generosity and hard work of a relatively small number of humans in the 1990's. I know as I was there.

[@mortemmalum7228]

​@@DanielSMatthews Yes they do sell Linux. The reason that Linux runs the internet is because of Redhat and Canonical. Both do a lot of marketing for their products. It's childish to think that anything can be done without money and marketing. Linux wouldn't exist(in the capacity that it does now) without an economically viable and marketable use for it.

[@ttyler333]

I think I need a big tutorial. However, I've had ideas but don't have the means to make them reality. What a great video!

[@AnotherByteData]

Thank you! You are an inspiration! A bio-hacker and an open-source guy! Kudos!

[@guterflex7967]

All of this is honestly so incredibly interesting for me, I really love your videos, they inspire me a lot. Since this is kind of mind blowing to me, I asked myself whether you could do a video on the current state of genetic engineering and where the field is at at the moment (what is possible already and what might become possible in the future). And also do you (or anybody else) know any online resources to get started with genetic engineering? Maybe online courses, other youtube channels, websites (like the ones you used here), a great textbook etc.? I can't even reall find the words to describe how inspired I am right now. And thanks a lot for sharing this knowledge with us!

[@Asrashas]

Regarding the short patent side note at the beginning: I think patents in some form are kind of necessary. You pour all your resources into R&D trying to get something to work. That costs you time, effort and money, which you should be able to make back. Usually by selling the result in some form. Now someone comes along and yoinks all the R&D (or some of it) from you and can offer the same product that you have for a lower price, since he doesn't have to calculate in any R&D costs. Or at least not as much as the original inventor. Though patents should ideally expire sooner rather than later. They should offer the inventor a small head start to compensate for his investments, to make those investments of time, energy and money potentially worth it. Not more, not less. That's my amateur/arm-chair take on it. Edit: You talk about it a bit more at around 2:43:00 And It seems after you do that I pretty much agree with your stance on those topics.

[@kylebowles9820]

It's important to show the search and struggle. That's how learning and real scientific progress happens. Lots of times students get used to the books and teachers having the information; plus how strict academia tends to be, it really sets you off on the wrong foot for your career.

[@SoulDelSol]

Exactly. In real life there is no answer booklet already prepared for you to compare your work against

[@RyanEbrahimi]

I wasn't able to join with the beginning of the stream this time, but I really enjoyed this. I'll definitely tune into or watch any stream you have.

[@Dunderpunch]

Pretty interested in getting my hands on some engineered yeasts, whatever interesting thing they end up making. Not sure I can do much with a plasmid, but I can brew with yeast.

[@kelpc1461]

ditto

[@notapplicable7292]

brew beer out of milk yeast? could be fun.

[@KarolOfGutovo]

@@notapplicable7292 Not quite, the yeast is modified to produce milk proteins instead of ethanol if i understand correctly

[@a2e5]

Karol Gutowski Beer making involves anaerobic respiration, which is a pretty basic part of yeast metabolism and can be quite hard to be removed. Same goes for yeast making bread puffy by literally exhaling CO2. If you put milk stuff into yeast and brew with it like any other yeast (you know, anaerobicly), it either makes milky beer because it’s alive or makes nothing because it’s dead.

[@xxportalxx.]

@@notapplicable7292 sounds like milk stout, but that generally uses the lactose not the protein

[@RandomJBerry]

I love this channel. one of the greats.

[@GaussianBluff]

Dang, I had no idea this is even possible. :D Any ideas for beginner projects with a decent amount of practice per dollar?

[@albino7846]

Yes good one. Where to start

[@atsitferell6876]

There's a video making glowing yeast from this guy, he explains it is the starting point. You can also get online kits

[@kleinesfilmroellchen]

I'm a computer scientist. I like your funny words magic man, but my takeaway is that genetic engineering is basically reprogramming organisms

[@sophiestrano]

As a fellow computer scientist and bioinformatics bozo, that is pretty much exactly what we would like to be able to do

[@ILLUMINATED-1]

this is funny because as an engineer of like 20+ years, I find it very very different. The lack of concreteness and reproducibility must be maddening, everything feels like a hack that's barely hanging on. Still very cool, but I'm not sure I could tolerate it professionally, lol

[@MrHeroicDemon]

@@ILLUMINATED-1 That's where I'm at right now lmao. "Could I tolerate this professionally."

[@pol9056]

@The Thought Emporium What was the resource you mentioned for people who are interested in getting started in genetics? Thanks for the help!

[@MrTheWaterbear]

Btw, glad you’re interested in a lot of things, but bioluminescent plants has been attempted and is a dead-end so far. Unless you can specifically express it in the outermost epithelial cell layer, or the stomata for example... Turns out that plant pigments are very good at absorbing almost all visible light. And it’s an important consideration for the viability of the plant only to express these proteins in darkness (or at night - these are not necessarily the same thing nor do they have the same requirements), which could be possible through a lot of complex modules. So challenge 1) Localize bioluminescent expression to avoid absorption 2) Avoid constitutive expression, and perhaps tie the point of induced synthesis towards the bioluminescent product to a molecule which is readily metabolized by the plant. This way, you have lots of the molecule natively synthesized, and you can also use all these molecules for energy or other syntheses when it’s daytime or there is sufficient light. Minimize the burden on the plant, essentially. As an ecology major who switched to a degree in synthetic biology and molecular genetics after graduation, I want either a robust kill switch for our plant, or sterile, dysfunctional gametes so it doesn’t spread uncontrollably. Of course, any idiot with some growth hormone could “steal” and reproduce the plant vegetatively, but who cares. It’s the random spreading that needs to be avoided :)

[@cel7691]

I don't really know anything about what you're discussing here but thank you for posting these long videos, they have been great to put on and listen to while working. Just a bit into the video, can't wait to find out what happens in the end.

[@weirdal3333]

He's ⅓ of the way to making automatic bread... All that's left is sugar, fiber, and a lot of heat. Automatic cookies should have extra vanillin genes.

[@stribika0]

You mentioned that some codons that oode for the same amino acid result in lower yield. I find this fascinating. You could use this mechanism to control the ratios of the milk proteins, essentially for free. Are these efficiency differences quantifiable? What's causing it?

[@stribika0]

So I looked this up a while ago, and it seems to be because of different tRNA concentrations. It is not known why the concentrations are the way they are, but changing it to something else seems doable. It would be more difficult to re-optimize every gene to the new setup, but if you did that, then every virus would suck at replicating in your organism. They would still work, just so slowly that they would be no match for the immune system.

[@xzendon]

As an engineer who regularly invents things, pouring my heart soul and time into it, if my creative efforts were just stolen by companies with more resources, I would be really annoyed. That being said, what big companies are able to do with patents is ridiculous.

[@mynicknameislion1385]

How did the court even allow that company to copyright human milk wtf

[@drdca8263]

I think technically it is specifically a copyright on a method of producing* human milk* . Not to say that that makes it justified.

[@guterflex7967]

Still fucked up, tbh

[@jimmysuryadi3017]

@~50 min, It is pronounced Nde - One, the I is roman numeral. The expression methods will make the proteins significantly larger because the N-terminal tag and the left over C-terminal from the P2A. For example, the beta-Casein MW ~29 kDa becomes ~40 kDa. Also the theoritical PI (using protparam), from 5.28 to 4.7.

[@SaranshKarira]

Hey man! Your content is gold!! I am from Machine Learning/Programming background but I like to dip my hands in a lot of things from robotics to mycology. I am currently going through this video and this is immensely helpful. I recently gained an interest in gene editing because of a "ridiculous idea" and found your codebase on github and navigated here through that. How would you suggest I start into this? Any resources?

[@themadpolymath3430]

Streaking out yeast while watching this haha. Thanks so much for your content!!!!

[@mamabun]

This is really interesting. Any good books to start with to learn how to do this? Also can you do this without the sequence for lactose?

[@letsgocrypto4971]

He suggested The Manga Guide to Molecular Biology (Paperback) earlier in the video!

[@derderrr7220]

adding referance notation helps with comprehension like having a key for maths, glossary for referance points. ease of understanding the lim. and boundary of the how and why so this kinda thing is used responsibly we don't want to chronumburg the place up.

[@doleber3918]

You defenitly earned the bell activation ! Great Video. I am excited for the followup.

[@divinephanes]

coming back to the thing that sparked my interest in microbio! applause to you and these videos, you are an inspiration to me

[@seanshomeshop325]

i will not eat red eggs and ham, i will not eat them sam i am

[@edvardstreijs9083]

Great video! I'm a chemist first and now I am realy getting into biology so I was just wondering... If you feed it just sugar where the is is going to get its nitrogen for the albumin? (amino = amine = NH2 group) Can you feed it fertiliser :D or amino acids or just nothing?

[@thethoughtemporium]

Normally you add "wine nutrient" or "yeast nutrient" which often contains urea and ammonium phosphate, as well as micro nutrients. This is especially important when you want things that aren't just ethanol. Basically you tune the media to what you want to produce. Or if you use feed stocks like fruit juices, grains, etc, it provides a lot of this on it's own, but adding extra nutrients increases yield. Another option is to add nutritional yeast which will contain all the nutrients and aminos they need. Really the trick to making this be awesome and not just extra work, is to use feed stocks that would otherwise be waste. Starches from noodle making is a great example. Or if you buy certain enzymes you can preprocess some feed stocks that would otherwise be difficult to use. And as long as you boil the mix before adding the yeast, they won't interfere with whatever proteins you are trying to grow as the heat turns the enzymes off.

[@HorochovPL]

Hey *Thought Emporium,* You may be interested in Vivaldi browser. Chromium based (as is Chrome browser), but the main advantage is you can display open cards on vertical panel (check it in options) and it's much more readable than crammed horizontal, shrunken cards. I.e. it shows almost entire card name: "Addgene: TEF1pro_mCherry_Y" (it cut "FP" ending with my card bar width). I'm not affiliated to Vivaldi, it may be more convenient to use by You in my opinion. Thanks for spreading the DNA engineering knowledge with us!

[@gahtannahdi8595]

Question, how would the yeast acquire the amino acids to produce the proteins? You said that you only need to feed the yeast with sugar to make it work. So can the amino acids be synthesized by the sugar? I'm new to this and would like to know

[@RUMPshit]

Do you know anything?

[@shithappensdealwithit4168]

2:10:05 What about cockroach milk?

[@mrmolasses5366]

its "milk" not milk I'm pretty sure milk is only milk if its from mammals

[@shafthespaceegg]

Dear Thought Emporium, what serotype of AAV did you use for construction of your lactose intolerance gene therapy?

[@haruruben]

This Is so interesting, now more to learn

[@fdbhiroshima8979]

I did not watch the lives because I read the title as something about ethology, like tracing where a gene come from in a phylogenetic tree. Thanks to your last video (about lactose intolerance) I just discovered this is one of the most awesome playlist I've come to see. This is great content and sadly I feel like the title is really bad at selling it

[@connorhoyle2926]

"Im kinda burnt out on coronavirus" *Cries in 2021*

[@brycemw]

He says to see the neuron update video on the Applied Science channel but I cannot seem to find it. Was it unlisted or something? Anyone have the link?

[@abhilasha9608]

I missed the last stream due to the time zone difference but I'm gonna make sure I watch the next one.

[@ShaneGadsby]

1:21:27 : Sas mayte, ya trippersnipper saves the day again!

[@paulj9587]

Can you speak more to the logging industry patent which makes plants grow faster - I'd love to know more.

[@Andreas-gh6is]

I think the most promising strategy in replacing animal food stuff like milk, eggs or meat is to use un-modified plants to provide the bulk protein and "spice it up" with synbio. For example the synthetic heme in the "impossible" products. Also eggs and milk are used for their food technological properties like binding together components. Plant-based lecithines often aren't as good.

[@Joh447]

is there a limit to how much yeast you can grow at once? like is there a differencd in prodedure between a 10ml dish and a ten thousand gallon tank?

[@BitchmansTROLLPLAYS]

Can someone explain to me how to ,,design" restriction sites? I thought from school that your insert needs to be inside those two dangly bits and your insert needs to match that from both sides to make a match so that it can anneal be put back together. Here it looks to me like the site is completely before the insert sequence and I dont quite understand it.

[@piotrfila3684]

Why are you using a browser gene editor? Are there really no good open source programs? It seems really slow for what it does (especially the insane loading times). It feels like a good editor could speed work up quite a bit

[@badassjack86]

Make a yeast that expelles the bitterness of hops, and the smoothness of malted grain so you can make beer only using yeast, and sugar water.

[@edvardstreijs9083]

sounds like a plan :D

[@idontwantachannelimjustcom7745]

Hamster isn't on the list is it?

[@ayush.kumar.13907]

are you saving that red milk for Halloween?

[@Andres-vd8uy]

honestly, I really hope he orders the egg yeast and cooks with it

[@monikachxr675]

I was watching one of your videos at night. I accidentally fell asleep and woke up to this 😎

[@namAehT]

I'm down for some Egg Yeast. Would be great if the yellow could be done too but you said it's a nightmare

[@Purwapada]

lol i just realised im watching this one the exact date this was made, but 2 years later wow

[@warro-jg2yq]

where do you get all the aminoacids from. dont you need a lot of it to synthesize that much protein?

[@SenorRu]

I wonder if the fake egg could be made flavourless. A lot of low-carb bread recipes use egg for binding things together but the taste often overpowers it.

[@bigsweaterman8142]

3:00:00 could you do a tutorial (or a series of tutorials) for beginners on how to use a program like this? Is that a thing that you can do or should I just go to college?

[@reverendissimo82]

i'm interested to the albumine yeast, are you doing a foundrising to make it? i'll be happy to put some money in it.

[@ekbergiw]

Can you describe the differences engineering algae as opposed to yeast?

[@jamesbrown99991]

So when can we buy dessicated Saccharomyces Caseiata from your etsy store? :)

[@MrTheWaterbear]

Your opinion on Biology degrees tells me you probably should try to get a degree in Synthetic Biology, Molecular Genetics or Biotech. Because here in Denmark, Ecology graduates have a rather high unemployment rate; whereas the more technical biology graduates such as the degrees I recommended have

[@allurbase]

They forgot llama too, just in case

[@letsgocrypto4971]

lol...

[@DecodingDoom]

Really wish you could do an HD reupload of this. So many details in your presentation and it's hard to make them out.

[@andrewmclean4212]

red/yellow milk a new coffee sensation

[@ProfGrimm]

Here is just a little tip for the next time u stream : press F11 Some people like it ,some not , but i think it's bedder to have more "screen" . I mean most of the time you don't need to skip around ... so it's just unused space . Ps: Nice Video , you explained it so well that even i as a 15 year old man understood it ;)

[@mikehoward5012]

Any updates to this project? Would be pretty neat to pick up some synthesized milk yeast at the local grocery 😁

[@markcangila1613]

I have a question about mechanism: how do you brew the milk after you have the yeast? Like, if I have yeast with the genes, what do I do with? Do you just put it into solution with sugar? Bur then doesn't it also produce ethanol still?

[@alexisflory6496]

Feed the yeast (warm water and some flour or sugar). Idk about the ethanol though.

[@Goodengelt]

Seriously, soulfood. I'm in awe.

[@PuresG1ft]

I‘m always surprised by the face that correspondents to the voice im used to

[@dmacki3521]

Has anybody tried the egg white yeast? I’d love to know what the yields are.

[@Goodengelt]

This was scholarly. 👍Sorry I missed it live 😔

[@deathzombee]

P2A sounds exactly like a NOP Sled used to skip instructions in Machine code (when hacking it), though the ejection part would be extra.

[@MrPolluxxxx]

When brewing proteins, is there a risk of producing proteins that don't have the normal folding and potentially produce dangerous substances (like prions)? Pls no bulli, I'm not a biologist.

[@haydennorris2913]

No. Prions are usually misfolded proteins called chaperones, specifically chaperones expressed in your central nervous system tissues. Chaperones normally help other proteins fold. Talking something like albumin that wouldnt normally help proteins fold and causing it to misfold would not cause it to misfold other proteins. Also these misfolds often require some kind of mutation (like heritable prion diseases) to be folded like that. Not giving the right conditions to fold may stop post translational modifications from happening and may cause a loss of function in your protein, but they will be degraded anyways.

[@MrPolluxxxx]

@@haydennorris2913 Thank you very much. Just so I understand, if misfolding would occur, it's very unlikely to make anything more than loss of function, correct?

[@haydennorris2913]

@@MrPolluxxxx thats right. And considering oval albumin doesnt do all that much to begin with it probably isnt that big of a deal that its misfolded, if at all.

[@fredo514]

A few ideas: - plastic degradation - fuel production - bioplastic

[@younesben556]

Tu veux pas jouer a dragon city plutôt

[@chaegibson720]

Hey if someone could help me out would be greatly appreciated I’m following along like a recipe for practice I’m at the part where he has 46815 plasmid, he clicks on Sall and hits paste, Sall stays in the program and he has 8135 base pairs after that and Sall doesn’t get highlighted, when I do this on mac(I have double strand on, I have codon usage set to yeast, I have the ovalbumin amino acid sequence, I have it set on CDS) but when I paste it doesn’t show Sall and the colour colour extends back into Smal, and I only have 8133 base pairs, please help I’m not sure what I’m doing incorrectly I think there’s a glitch either on my end or yours, will it still work? Help appreciated

[@viralnow8008]

Same here

[@nickmagrick7702]

7:17 thank you, I also think owning an idea is most often, absurd.

[@0x90meansnop8]

produced protein as inhibitor for producing the color? this way you could keep a small amount of your color

[@codyplaisance7281]

i actually had a biotechnology class in college that went over this in some detail

[@migkillerphantom]

genetic engineering looks like trying to create software only using #include tags and random header files you found on the internet

[@IM-lf5qp]

I fell asleep to a Mark Felton video, woke up to this

[@JWSmythe]

Question about all this, related to space travel. In various movies, TV, and video games, they frequently say that growing food will be difficult to pretty much impossible. Sometimes it's a plot point, and sometimes it's just included. Would this kind of process be scalable so people at the Mars colony (assuming Musk is successful) wouldn't just be eating fungus, potatoes, and 10 year old MREs shipped from Earth? It would seem that what you're demonstrating wouldn't just be a few pre-picked foods, but (almost) anything. You're making animal proteins from yeast, it would seem that the process could be used for almost any kind of food stuffs. I would understand why they haven't attempted their own food supply on the ISS, due to space constraints, but a Moon or Mars base has local materials, and room to grow. I'm sure they've done small scale experiments, because they've done small scale experiments with everything.

[@Ryan6.022]

I hate I missed this when it was live

[@cillyhoney1892]

Tell me more about this THC producing yeast. I would like to buy some.

[@666aron]

I have a feeling that I could only use Genome Compiler after minimum 5 beers. It runs on flash, won't let me rename folders, somehow I can't paste amino acids like you did. OR maybe I was too tired, and did something wrong.

[@chaegibson720]

Aron Feher I couldn’t post the amino acid either without it fucking up the sequence

[@chaegibson720]

Aron Feher lets say we’re at like 1:38:17 you’ll see he has 8135 base pairs I keep getting 8133 base pairs

[@byGDur]

Are there updates for this project? Would be so cool to make this a reality :)

[@markcangila1613]

Genecompiler is going down. Do you have any other free suggestions?

[@pavelkryl457]

I'd try it with moufflon milk (if I had the money), just because I think it sounds cooler.

[@EvanBoyar]

How about cilantro that produces bitter saponins so it tastes like soap for EVERYONE? I want the whole world to suffer like me!

[@jadenpeterson4881]

Evan Boyar some people enjoy bitter flavors. Black coffee, dark chocolate, IPA beers, spring greens. I would try it!

[@altaccout]

Patent US 6423509 B1 for yeast that makes human breast milk expires today! Cant wait to start my first bioengineering project.

[@SahasaV]

Imagine patenting human components..