Making and Using a Spore Syringe (for mycology)

  Рет қаралды 45,820

GordoTEK

GordoTEK

Күн бұрын

Пікірлер: 131
@shareeverar9420
@shareeverar9420 Жыл бұрын
There are so many incredibly detailed steps, thank you so much for this informative video. Very thoughtful and thorough.
@filippoarlenghi6247
@filippoarlenghi6247 Жыл бұрын
The acquarium heater idea is super clever
@sherrypayton6871
@sherrypayton6871 7 ай бұрын
Great ideas! I’ve watched a lot of videos and I have been in this hobby for over a year, but I didn’t know about the horse grain. Thank you.
@kwahdomo9598
@kwahdomo9598 3 жыл бұрын
You are the man Gordo 🙂
@xj11w2c7
@xj11w2c7 2 жыл бұрын
Yo dude thank you so much for letting me know about getting syringes from tractor supply. I'm working really hard not to support Amazon and just been looking for syringes all over
@nathanw5747
@nathanw5747 11 ай бұрын
instead of the acquarium heater . you can also use a plant seedling heating mat + a PID temperature controller . vivosun has the mat + temp controller for like 25$. i happen to have all this i grow superhot peppers as a hobby . so if you do not have fish this maybe a option with dual use .
@JoeBlan-k8g
@JoeBlan-k8g Жыл бұрын
Glad I found you! I'm just getting started and you are full of great information
@3684langley
@3684langley 27 күн бұрын
You answered one of my questions about sterilization of previously used spore syringes, ❤❤❤
@R.l.g.R.l.g
@R.l.g.R.l.g 5 ай бұрын
New grower here just wanted to say hello and thank you for all the info it was very very helpful
@derby99ultra
@derby99ultra Жыл бұрын
I've been really struggling to find a reputable source for my first spore print in the uk. I'm gonna take a peep at that patreon now. Thanks gordo.
@gordotek
@gordotek Жыл бұрын
You are on the right path ;)
@420Bill
@420Bill 3 жыл бұрын
10:40 you might be able to use a hair straightener to seal the bag as well
@gordotek
@gordotek 3 жыл бұрын
I've heard of people using a hot iron, or even heating up a metal coat hanger on a stove and using that to seal it (probably not a great idea).
@myotheraccount5947
@myotheraccount5947 Жыл бұрын
Tractor supply.... you must be nearby me! I'm new to mycology and it seems I've gotten way too much poor information from KZbin channels. You're video here makes things much simpler than others and gives me hope. I'd pay you for an in person consultation if you're close enough and willing
@gordotek
@gordotek Жыл бұрын
Private message me on patreon.
@smokedaddy773
@smokedaddy773 Жыл бұрын
This video has been amazing g and so much help. Thank you for the detail
@profetarmageddon
@profetarmageddon 3 жыл бұрын
I appreciate your information!!!
@toddwmac
@toddwmac 2 жыл бұрын
Thanks man....really appreciate your contributions.
@starchylde4538
@starchylde4538 Жыл бұрын
Ty fir your knowledge!! Awesome channel!
@LoveOverWar-l9v
@LoveOverWar-l9v Жыл бұрын
THANK YOU
@Lemonz1989
@Lemonz1989 Жыл бұрын
A tip for sealing unicorn filter bags if you don't have an impulse sealer: I use the seal function (without vacuuming) on my cheap vacuum sealer machine. It works fine. :) I don't know how much an impulse sealer costs in the US, but here in Denmark they can be really expensive - some cost hundreds of dollars.
@gordotek
@gordotek Жыл бұрын
Good idea. FYI you can get an impulse sealer on Temu for $20
@lanthanumlanthanium6373
@lanthanumlanthanium6373 Жыл бұрын
@@gordotek Don't support that country
@IvanLopez-ed4ti
@IvanLopez-ed4ti Жыл бұрын
Do you have a video of what to do once you begin the mushroom colonization?
@gordotek
@gordotek Жыл бұрын
Yes seem my FAQ link is in the description.
@gordotek
@gordotek Жыл бұрын
www.patreon.com/posts/22774475
@anthonyroman218
@anthonyroman218 Жыл бұрын
May I please chime in regarding your laminar flow hood, so I noticed you have the inoculation set to Hight correctly within the correct parameters of your laminar flow except for one thing is that you have your inoculation site on a upside down Pyrex plate, which does not allow proper air pass through through the bottom and underneath of the Pyrex plate , you have placed upside down, which may result in a upward draft of your laminar flow. may I suggest a wired basket turned upside down instead of using a solid glass Pyrex, which may introduce a upward draft of turbulent air from the bottom part of your laminar flow setup remember turbulence is always your worst enemy when it comes to laminar flow I also noticed you have Foil that has been stuffed between the filter itself and your inoculation site on the upside down Pyrex plate to reduce this updraft so it is apparent that you are attempting to address this to a certain extent, but just to be on the safe side, I would rather use a wire basket and adhesive glued to a piece of glass or even some acrylic or something that is flat that you can glue on top of the wire, upside down basket to create a solid platform to work on and to remain free of worry of any updraft because of the wire basket will allow air pass through and even better no need to worry about stuffing the foil between the pyrex plate and your filter anymore🙃🙂🙃😎
@gordotek
@gordotek Жыл бұрын
You are very observant, and absolutely correct! I actually have a separate video on building your own laminar flow box where I do a better job, and use a proper wire rack, haha. But that laminar flow box in this vid is a massive commercial unit that is 2x4 feet in size, the sheer size and flowrate pretty much ensure no matter how silly my platform is, its unlikely to have a problem. But still, you are correct, I should be a better example.
@GeeZOhio
@GeeZOhio 2 жыл бұрын
Awesome video
@urbanforager90
@urbanforager90 Жыл бұрын
I want a spore print please
@gordotek
@gordotek Жыл бұрын
contact me on patreon
@stevenamgad2339
@stevenamgad2339 Жыл бұрын
Thanks
@theomarimira1
@theomarimira1 25 күн бұрын
Hi Gordo. Do you think it would be clever to use sawdust rather than grains to minimise growing other organisms. I've tried making spore water before without much success. I was trying the grey oysters and their prints are practically invisible on foil.
@MyTube4Utoo
@MyTube4Utoo Жыл бұрын
Fantastic! Thank You.
@serdarguler2340
@serdarguler2340 2 жыл бұрын
Sory 🙏 Do you sell Psilocybin mushroom spores?
@gordotek
@gordotek 2 жыл бұрын
No, but I give away free prints to all patrons at any tier level.
@obasi_12
@obasi_12 2 жыл бұрын
I will refer you to an online psychedelic store where you will get Psilocybin mushroom spores,dmt,lsd chocolate bars edibles!!
@obasi_12
@obasi_12 2 жыл бұрын
sam_trippz!!
@obasi_12
@obasi_12 2 жыл бұрын
On insta
@serdarguler2340
@serdarguler2340 2 жыл бұрын
@@gordotek Thank you for your interest
@timothykramer1856
@timothykramer1856 Жыл бұрын
Thanks mush❤
@vincentwu2848
@vincentwu2848 Жыл бұрын
Thanks for sharing such an informative video! Out of curiosity, is it a bad idea to wash the spores off of the foil instead of scraping it off with the scalpel?
@gordotek
@gordotek Жыл бұрын
You can do it that way or even form the foil print into a cup and add water directly to the print, swirling and scraping it up with the syringe needle if you want (don't poke through though!)
@vincentwu2848
@vincentwu2848 Жыл бұрын
@@gordotek Oooh thanks for the advice, I've never done prints before so I have no idea what to expect. I figured if its powder on a sheet of foil, squirting water from a syringe would wash it right off but based on your feedback it sounds like it will take some agitation with the needle to get the spores off. I use 2oz condiment cups for agar, do you think it would be a good idea to try to take spore prints with a condiment cup instead of foil? Then I can place a lid on the cup after the spores drop, and then inject water through the lid and draw up spore solution..?
@pieterplant926
@pieterplant926 Жыл бұрын
Awesome!
@ascar34400
@ascar34400 Ай бұрын
Hello Gordo! I'm beginner and i bought your ttbvi kit here in europe. I have few questions because there is no instructions for the grain inoculation process. I shoot the entire sringe in the grain bag, then i put the bag at 28 degrees celcius and wait for full colonisation? Other question, i also work with tricoderma (for organic soil, box extraction filtered by carbon filter). Do you think i should make a laminar filter to avoid contamination by filtering air in the room i grow mushrooms? Thank you very much!
@gordotek
@gordotek Ай бұрын
The minimum to inject is around 3 squirts of 0.5cc each so 1.5cc total, but you can double that. You will likely have extra, its OK to use a bit more but probably a good idea to save the extra for backup purposes or to inoculate additional grain or agar. 28C is a good incubation temp. A laminar flow box is GREAT for doing actual mycology work including agar work or grain inoculations. Its not really designed for continuous running to clean the air in an entire room or house. For that I would recommend a corsi-rosenthal box, there are lots of vids on here showing how to Build a Corsi-Rosenthal Box
@lbar2458
@lbar2458 Жыл бұрын
Uhh i would like a spore print. I ordered a syringe but it seems to have more contams than spores.
@gordotek
@gordotek Жыл бұрын
I used to give them to anyone that asked but I've been overwhelmed and can't keep up anymore, sent out more than 1000 prints, so I had to cut back, but I STILL give free prints to all patrons at any tier (as little as $2 this basically just covers postage). Giving out the prints I set a world record with and won the Cultivar Cup with).
@ricardokjf
@ricardokjf 8 ай бұрын
Hey loving you videos an way of explaining this all, first class. I'm very new to this, so was wondering if that free spore sample is still available??
@gordotek
@gordotek 8 ай бұрын
I used to give prints to anyone that asked but I've been overwhelmed and can't keep up anymore, sent out more than 2,000 prints, so I had to cut back, but I STILL give free prints to all patrons at any tier (as little as $2 this basically just covers postage). I'm actually sending out two prints now to every patron, and they are arguably the best genetics in the known world. You can't be that. I just pay it forward and hope enough people will appreciate the content and stick around to make it worth doing. Also note there is a community chat now on both the patreon app and website and the GordoTEK chat has been super active with everyone helping each other out, great vibe.
@gordotek
@gordotek 8 ай бұрын
www.patreon.com/GordoTEK is the link for patreon
@UGSETH2
@UGSETH2 Жыл бұрын
Could the aquarium heater be used throughout the entire growing period? I loved the idea and can't see why it couldn't be :) Peace
@gordotek
@gordotek Жыл бұрын
Yes it can.
@recyclingnik
@recyclingnik 2 жыл бұрын
Thank you for the good and informative video! How long would you cook de hulled polished barley grains? I have those at hand..
@gordotek
@gordotek 2 жыл бұрын
Sometimes the hulled versions don't work very well, they become mushy fast. But you can give it a try. Maybe just pressure cook them for 30-40minutes.
@recyclingnik
@recyclingnik 2 жыл бұрын
@@gordotek yeah that`s what happend ! it worked somehow and the growth was more aggressiv in the jar but breaking it appart was a pain because it was mushy and sticky..won`t recommend hulled grains
@plamenconev3387
@plamenconev3387 Жыл бұрын
Hello, I have a very important question. I am considering using the following ingredients: Rye grain, parrot seed mix, brown rice flour, vermiculite. My question is this: Will it be successful after colonizing my jar (possibly, I would hope: ) then directly transfer the colonized substrate to the fruiting chamber and add only - first layer of perlite, middle layer of mycelium, upper layer of vemiculite. Are these nutrients enough? thanks in advance
@gordotek
@gordotek 7 ай бұрын
That might work, you should check out my patreon page for lots more info and Q&A plus an active community chat where people help each other out, its all FREE.
@justinturnbull5466
@justinturnbull5466 Жыл бұрын
would love a spore print how do i message you
@gordotek
@gordotek Жыл бұрын
Best way is on patreon, private message.
@plamenconev3387
@plamenconev3387 Жыл бұрын
hello friend, one print in how many milliliters of water is added?Also how many milliliters are injected in a liter jar? thanks in advance
@gordotek
@gordotek Жыл бұрын
You can get away with as little as 0.5cc of spore solution if you shake it up good and ensure there are spores flying around just before injecting, but most of the time you want to do at least 2 or 3 injections so that would be 1 to 1.5cc minimum per jar (doesn't matter the jar size). You should see growth in the jar after putting in incubation temps for about 3-5 days, once it colonizes about 1/3 of the jar you can shake it up and spread the mycelium everywhere, then it will rapidly colonize all the grain (only do the shake up one time).
@nickfranco2259
@nickfranco2259 Жыл бұрын
Do you sterilize those Luer locks?
@gordotek
@gordotek Жыл бұрын
You can indeed if you need to. They should come sterile at least for the first time use, but yea I sterilize and reuse them.
@smokedaddy773
@smokedaddy773 Жыл бұрын
Is it to late to get the free spore print sir?
@gordotek
@gordotek Жыл бұрын
No I'm still sending out prints. Just message me on patreon: patreon.com/GordoTEK
@bruhmomente7324
@bruhmomente7324 2 жыл бұрын
Hi Gordo!, quick question, I have multi spore syringes, & was wondering what the best course of action to duplicate them would be?, should I just transfer to agar & isolate, then inoculate to grain to get a spore print?, or just use the isolated agar to make liquid culture? Much thanks
@bruhmomente7324
@bruhmomente7324 2 жыл бұрын
Also, would CVG with calcium hydroxide work for panaeolus cyanescens, and do they require special agar, or would malt agar work?. furthermore will a liquid culture comprised of 500ml purified distilled water, 20gs 1:1 dextrose, golden malt, & 5gs peptone be a good liquid culture mix for panaeolus cyanescens?
@gordotek
@gordotek 2 жыл бұрын
You can either direct inject to sterile grains or put the spores to agar, let it grow out, then transfer that to grains. Then apply the grains to substrate and grow it out to obtain more spores from mature fruit bodies. You can also use liquid cultures if you want, I don't like LC personally, easily contaminates and you can't always see that it is contaminated.
@christoffergustafsson3057
@christoffergustafsson3057 2 жыл бұрын
I would like a spore print 😊
@Eddielongan
@Eddielongan Жыл бұрын
Question,I'm having trouble understanding or answering by watching videos...... after you boil your grain, strain then sterilize, is the grain completely dry when you put it in jars and add your spores,or is it wet?
@gordotek
@gordotek Жыл бұрын
So, the grain itself absorbs water, it will look all "puffed up", there is lots of water inside, but not that much water on the outside. It shouldn't look very wet, for example there shouldn't be a puddle of water on the bottom of the jar. When you inject the spore water that will provide just enough extra water to get the spores germinating.
@jessicastacy211
@jessicastacy211 2 жыл бұрын
Do you have a video on how to make Changa? I prefer Change to crystals. Thanks in advance!
@gordotek
@gordotek 2 жыл бұрын
Its very harsh on the lungs and even the guy that invented it says he no longer uses it, haha. I don't recommend it but its very simple, just combine the DMT and MAOI containing plants and smoke.
@ninjashrine0292
@ninjashrine0292 Жыл бұрын
is it too late for a free spore print???🙃
@gordotek
@gordotek Жыл бұрын
I am still giving away free prints to all patrons, its as little as $2 to join and that barely covers my costs. There should be a message icon on the patreon app and on their website, put GordoTEK in the to box and I should get it. Or click on my name and then there should be a message link.
@benink5690
@benink5690 Жыл бұрын
So the print isn't contamed? Anyway to take a sterile spore print?
@gordotek
@gordotek Жыл бұрын
If the print was prepared in front of a flow hood, its likely very clean, I rarely see contams coming from prints although its possible. If the person who made the print didn't use a flow hood, its very likely full of contamination.
@jimmcdees1341
@jimmcdees1341 Жыл бұрын
Bro I wish I could afford to become a patron atm! I'd help support a few of our family! I'm just a single dad with custody of my youngest daughter and do it on my own! It's why it's taking me so long to get into the mycology... Everything is expensive 😂 mush love man! I'd love to know how to get a spore print without being a patron
@gordotek
@gordotek Жыл бұрын
Well its only $2 to join and I give spores to all patrons so that would be one way to get spores for next to nothing ;) I wish you well in your situation, I imagine that is quite challenging 🙏❤
@Aqua1111-sm3qx
@Aqua1111-sm3qx Жыл бұрын
"Wouldn't there be any contamination issues for a spore print to pass from the United States to Japan? I'm curious if it's possible to make a syringe from a spore print that comes in a zipper bag and is shipped in a box...
@Aqua1111-sm3qx
@Aqua1111-sm3qx Жыл бұрын
"All preparations have been made, but it seems impossible for me to bring this to Japan..."
@gordotek
@gordotek Жыл бұрын
No, I've actually sent several prints to Japan (and all over the world to more than 70 countries) with no problems. Mail to Japan is even very fast too. If you want prints contact me on patreon.
@Aqua1111-sm3qx
@Aqua1111-sm3qx Жыл бұрын
@@gordotekgod's grace...
@Aqua1111-sm3qx
@Aqua1111-sm3qx Жыл бұрын
@@gordotek "How long can foil-wrapped spores remain contaminated and unusable?"
@Clyffcarlock1
@Clyffcarlock1 2 жыл бұрын
How much water in the pressure cooker? Where would you put the cup of water to be sterilized?
@gordotek
@gordotek 2 жыл бұрын
Follow the directions for your cooker, the quantity depends on the size, but generally there will be some removable platform that you put the items you want to sterilyze on top of, and the water fills up to just below that platform.
@lucaripoli2717
@lucaripoli2717 Жыл бұрын
If you leave it in jar it will produce sclerotia?
@gordotek
@gordotek Жыл бұрын
Only certain cultivars will do that, I am not a fan of this type of grow as the sclerotia are slow to grow and "dirty" when harvested, usually harder to grow, just seems annoying to me with no particular benefit other than some weird legal loophole in the Netherlands where sclerotia get special protected legal status for inexplicable reasons, haha.
@csucsucsucsuka8356
@csucsucsucsuka8356 2 жыл бұрын
Love you men! Superb.content
@sydneywalker2966
@sydneywalker2966 2 жыл бұрын
What gauge needle is recommended if you don't buy spore syringes specifically?
@gordotek
@gordotek 2 жыл бұрын
Pretty much any guage will work, even big blunt tips work. The spores are tiny and can pass through any size needle. The tractor supply syringes are 22 guage.
@nickfranco962
@nickfranco962 2 жыл бұрын
Is it dangerous to put that bag in on the top? Say if it breaks or opens up and clogs the steam outlet hole.. Can't that make a bomb?
@gordotek
@gordotek 2 жыл бұрын
Most pressure cookers have backup relief valves, but you do not want anything touching the steam vent. Some people put metal rings or other things on top to ensure nothing can clog it.
@SconnerStudios
@SconnerStudios Жыл бұрын
My only criticism is you are a little quiet. Your videos are really helpful.
@gordotek
@gordotek Жыл бұрын
Noted! ;)
@juliansullivan102
@juliansullivan102 2 жыл бұрын
Would love a spore print
@gordotek
@gordotek 2 жыл бұрын
Send mailing address by private message.
@juliansullivan102
@juliansullivan102 2 жыл бұрын
@@gordotek where will I find your email address?
@thederpguidetolife3014
@thederpguidetolife3014 Жыл бұрын
If I don’t have a pressure cooker what can I use? And how?
@gordotek
@gordotek Жыл бұрын
You can pasteurize things in a pot of boiling water, just requires much more time and is less reliable.
@johnbauby6612
@johnbauby6612 Жыл бұрын
WHere are all these low prices coming from? I bought a bag of oats at TS last week for double the price you published. Could they have doubled in a year or is it the state you live in?
@gordotek
@gordotek Жыл бұрын
You can search for a graph of the price of oats, what happened was actually pretty amazing, yes the price actually went up 200% in a year but has since fallen back down quite a lot this year. But with inflation in general, prices of everything will probably keep creeping up every year (hopefully not as much as oats have!)
@dustinkeller4595
@dustinkeller4595 2 жыл бұрын
I just have to say ty.
@parvathiven6133
@parvathiven6133 2 ай бұрын
How to order spore prints from you
@gordotek
@gordotek 2 ай бұрын
Best way is to go to my patreon page, sign up for as little as $2 and you will get prints (direct message me your mailing address). This gets you the top genetics in the world, can't beat it! There is also a totally FREE active community there where you can ask questions and people help each other, very good vibe.
@gordotek
@gordotek 2 ай бұрын
www.patreon.com/posts/frequently-asked-22774475
@amarmeriout7707
@amarmeriout7707 Жыл бұрын
Hi man your content helped me very mach after lining about psychedelic I desided to start growing them but unfortunately I live in Algeria were there are no spors around I would really appreciate if you helped me to get some I believe in psychedelics and Im rady to do whatever it takes to make this work kan you help me to get some spors in exchange I'm ready to do what ever it takes
@gordotek
@gordotek Жыл бұрын
Just contact me on patreon: patreon.com/GordoTEK
@zacm4740
@zacm4740 2 жыл бұрын
I remember Gordo one of the 1st if not the 1st to show how to grow mushroom in bulk like 5 years ago then Joe Rogan started talking about dmt then Gordo did tell using wood chips then Cris cantelmo did a video and mentioned Gordo's tek Gordo even left him a message in his comment section then Cris went crazy and capped himself poor guy. I remember though Gordo I remember I was watching it as it all went down you taught me a lot what happened to your videos on mush and dmt?
@gordotek
@gordotek 2 жыл бұрын
Greetings, the good news is that all of my original content is still available, follow the link in my vid description to the FAQ, at the top there are links to the old content that was banned. Cantelmo was a sad story of mental illness, some actually think he was murdered but I doubt that. The craziest thing about his story is that he had been trying to contact the FBI about a conspiracy to infect the world with a dangerous pathogen, this was literally just a couple of months BEFORE the covid pandemic began! Ah well, wish he could have found the mental health help he needed. For what its worth, I advised him to STOP USING ALL psychedelics and to seek some help but I could not get through to him. He was clearly abusing DMT which I do not condone in any way.
@gordotek
@gordotek 2 жыл бұрын
www.patreon.com/posts/22774475
@aldovarela3234
@aldovarela3234 2 жыл бұрын
Print?
@gordotek
@gordotek 2 жыл бұрын
Contact me on patreon, FB, IG, Reddit, or Twitter
@yt7927
@yt7927 Жыл бұрын
Only 3 things in life I want. A Lamborghini, A beautiful woman with a spectacular ass, and a spore-print from Gordo!
@urbanforager90
@urbanforager90 Жыл бұрын
How to
@girththeogre
@girththeogre 9 ай бұрын
Thank you for a great video! Very informative!! PS: Subbed!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
@dustinkeller4595
@dustinkeller4595 2 жыл бұрын
Roseburg
@docphillips3291
@docphillips3291 Жыл бұрын
🍄🍄🍄
@Bob-gn8ph
@Bob-gn8ph 2 жыл бұрын
You are awesome Gordo.. Jesus God bless you:-) Luke 21:36 KJV:-)
@Anonymous-ru2wk
@Anonymous-ru2wk Жыл бұрын
Gordo means fatty in Spanish FYI
@jpinksntc
@jpinksntc 2 жыл бұрын
Print
@gordotek
@gordotek 2 жыл бұрын
contact me for free prints
@robertoroman9125
@robertoroman9125 2 жыл бұрын
Print
@gordotek
@gordotek 2 жыл бұрын
contact me for free prints
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