Making Truffle Trees for the Farm - Tuber Melanosporum

I have created around 150 Tuber Melanosporum Truffle Trees for my farm. Information about creating trees infected with mycorrhizal fungi from the truffle family is hard to find. So I have decided to wing it and see how i go about with a method I thought might work. I have used dried melanosporum fruit body, ground up and added to a slurry of wet perlite. From here Holm Oak acorn seeds were placed in the slurry and allowed to germinate. Once they germinated I moved them to pots of soil.
The soil I amended with calcium carbonate to raise the ph to a level suitable for truffle species. The ph should shift to around 7.5, given time.
I am unsure if this experiment will work, but fingers crossed it does.
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[@OakandSpore]

Want to see how much revenue we make from selling mushrooms? checkout this link 👉 www.oakandspore.com/farmforprofit 👈

[@unclejess8742]

Any updates? Looking at doing the same thing on our farm. Can’t wait for your results!

[@JahaJaho]

Yes, curious to see if this works.

[@eriksonyw]

Will be at least a few years to find out

[@johnbrzenksforearm8295]

There's a fella with the last name Garland here in the States that has been successful at growing them for over 20 years. Emerald Lagasse use to buy everything he grew. Anyway, he sells the "sick trees", and now offers a non disclosure workshop which would be good for you but not us. Lol

[@madaboutnikkie6040]

Thank you !

[@FreshfromtheFarmFungi]

Cool glad to see it in action!

[@JustAnotherMaker]

Great idea. Very interesting. Looking forward to the results.

[@OakandSpore]

Thanks!

[@garethlfoster]

Wow, it's a good idea. It will be interesting to see your results. I will be following closely.

[@tamara8088]

Very exciting. Really looking forward to your next update.

[@OakandSpore]

More to come!

[@Throwingness]

WELLL?@@OakandSpore

[@dariazhempalukh]

@@OakandSporewe’re waiting excitedly! I would love to use this method to help repopulate endangered edible mushrooms in local forests!

[@santeripaattimaki1632]

This idea seems to be awsome. I cant wait to see more.

[@OakandSpore]

Hopefully it works!

[@newton9837]

I have contemplated this for sometime. if this doesn't work, try either starting the spores, or clonal tissue in oak root extract agar. Meanwhile try to start an oak cutting in a sterile tissue culture media. Once the roots have formed transfer the cutting to the extract agar, or vice versa.

[@yunusemregokmen6330]

Does it work and can you please elaborate?

[@newton9837]

@@yunusemregokmen6330 never tried

[@newton9837]

@@yunusemregokmen6330 the purpose of the oak root extract would be to provide it with whatever essential element that is missing in traditional mycological agar mixtures so that it could begin the growing process.. essential my idea would be to get some oak roots. put them in a blender and grind them up pretty finely and mix with some plain agar. Pressure cook the mixture, pour into petrie dishes, and inoculate the dishes with truffle slurry once the mixture is cool and solid in the dishes. Hopefully the truffel spores will begin to form a mycelial mat in the dish. Next would be to root an oak tree cutting in a plant tissue culture media, providing it doesn't have any anti fungals, I suppose it would work with soil rooted cuttings as well. Once the cutting has established roots you'd make mycelial transfers from the dish to the rooting media close to the roots. Hopefully it would form the symbiotic link and grow with the tree.

[@yunusemregokmen6330]

Thanks a lot will read again when I wake up and see if anything I missed :)@@newton9837

[@deepspirit7550]

Awesome video and experiment. You are brave to test this and see results in a few years :) Good luck, hope to see updates.

[@Ballskinicus]

what an inspiration. thank you from AZ

[@OakandSpore]

Thanks from NZ!

[@paullazazzera8461]

So cool! You're the man. I love your videos 🍄❤

[@OakandSpore]

Thanks mate!

[@geoffp9941]

I would say there is plenty of information out there but it is in the form of research papers. These tend to be really hard to read for the average person. In order to obtain a pure culture, there is a lot of equipment and techniques that have to be used. It is typical to find bacteria and yeasts within the gleba so creating an plate that inhibits those and promotes truffle mycelium is critical. I am interested on how this turns out though and will keep an eye on your channel

[@elijahjaugust7092]

Where is part 2!!!! I wanna see the results

[@shamefurudispray4193]

Looking forward to an update on those huge oaks in 100 years ;) Even if it doesn't work you'll have some nice trees.

[@OakandSpore]

"Alexa, set timer for 100 years"

[@tylerhall6455]

was thinking the same thing lol. Hes gonna be dead before truffels are growing on those oak trees.

[@monaletourneau7077]

@@tylerhall6455 Ridiculous reasoning. Truffles appear in 6-10 years.

[@tylerhall9412]

​@@tylerhall6455 hey, you stole my name. lol

[@garyssimo]

I will be 170 yrs old by then!

[@MycologyExploration]

So cool!!!

[@OakandSpore]

Thanks

[@tyo6896]

Motivated, love it. I'd be nervous about the sprouts, it's okay to germinate and innoculate.?..?I could see how this could be fine, but I would think it would eat the acorn.

[@jesidillon4593]

Never got to do this, although my idea was to wait a tad longer to attempt to inoculate, for no part reason. Would love an update soon

[@edmit2001]

Thank you for sharing, I think some Truffle Tree sellers they grow the tree in the bag to get full strong roots for awhile before infected them. Not sure if it actually work.

[@sergionascimento5365]

wow.... insane! You can try a fell bags using no pasteurization soil.... that soil you can take from the "tree foot", maybe the mix of microorganism can help the truffle get sucessfull! *sry for my bad ingles! Hello from Brasil!!

[@hergussmith3177]

This is so cool! Keep us updated. I wonder if it would be worth it to mix more spores into the soil as well

[@OakandSpore]

Yes, definitely

[@gettended]

@@OakandSpore 👀

[@davefstein]

Great video. I too would love to know if it worked. I tried the perlite mixed with ground up dried “spores” I bought on Amazon from Ukraine lol. Unfortunately after a couple of weeks the perlite and acorn slurry turned rancid and extremely acidic so it must’ve rotted or caught an airborne organism. I kept the acorns which had not sprouted but tossed the rotten slurry. I understand it takes 4-5 years to see any results of this experiment.

[@user-no3zs6ww8i]

Hi! Looks like good idea, but I have some questions: 1. Why do you pasterise soil? It seems be better atmospheric sterilisation for 3+ hours to kill concurent spores... 2. Did you apply some enzymes for better truffle spores propagation? There is an opinion that truffle spores have too tight capsule and digestive enzymes make it softer for easier propagation. In wild nature pigs and hogs are animals that help truffles to find new trees but only after passing their digestive system.

[@trufflesnuffle3694]

I have never used sterilised soil for my truffle tree inoculation. So far i have very well inoculated trees 600 being planted this year

[@Cander9966]

Any update on how the trees are growing? Would also love to see a step by step video on the germination process :)

[@OakandSpore]

I am waiting until they can be checked, which i will do in spring this year.

[@yeahnahsweetas]

​@@OakandSpore Any news bro? Please give us update video when you find the time

[@caragalka8288]

So, any updates yet? I'm so curious!

[@AnnaAndrewsMills]

Did you ever send a tree off to the lab and look at the roots under a microscope? Didn't see a follow up video on your channel, I was hoping for one reporting great success! :)

[@farminfabrication2208]

So did it work in the end?

[@ericpalmer1491]

was the slurry just to distribute the spores, or did you see any mycelial growth?

[@SweebahNZ]

I came for the truffles and stayed for the Slav squat

[@OakandSpore]

I do love a good slav squat

[@garyssimo]

Im 71 and can do a one legged squat. Gonna show my young MD to impress her....wheres my Cordyceps? they are great pre workout with co E Q 10.

[@mattbartels4598]

Hey mate how are these guys going? Very interesting hope they really take off for you!

[@OakandSpore]

Going well. I'll get them tested for mycorrhizal activity at around 2 years of age

[@mattbartels4598]

Ah nice you would probably want them in the ground by then to so you don't stunt them yeah? They will be hard to keep alive next summer in those pot unless you water the a 2 to 4 times a day But very cool little side hustle if it takes off for you

[@johnbrzenksforearm8295]

You ought to get some white truffles like the ones that grow in the Northwest USA and see of you can experiment with those as well

[@dieodd9866]

Well it is a good idea & i wish you the best ..i live in France & here black truffles are growing on the roots of old Oaks trees...many people have tried to replicate the europeen black truffle with no nor littl success & non equivalent in flavors..interested to see how it 'll evole.

[@OakandSpore]

Hopefully my experiment works.

[@drcobb84]

its been nearly a year, Im very intrested, did have you sent any of these trees off to the lab yet? amd if so, how'd you go?

[@OakandSpore]

Not yet, I spoke to the guy who said to check them at year two generally. I'll speak to him again soon..

[@drcobb84]

@@OakandSpore ahh keep me updated, :D good luck

[@boredbeingbored676]

thankyou im excited to see if that works

[@dyscea]

Another year? 😭 kk

[@michaeladamstone2911]

So when do we get to see the trees

[@staceyrichards4212]

Hello from Washington State. How are your oak trees going? Did the procedure work? Love to hear from you.

[@OakandSpore]

They are still growing! Will do an update video shortly..

[@garyssimo]

If there is ONE genius in all things myco....its Paul Stamets in Washington state.

[@hubertuskuhn9230]

How is the projek going?

[@edpickwell6519]

Hi mate did you hear back from the Lab to if your trees had the fungus?

[@OakandSpore]

Haven't had them checked yet. We are just coming out of winter, so they are finally sprouting.

[@edpickwell6519]

Hi mate did the Trees end up inoculated with the Truffle spores from last year?

[@debireed6128]

Any updates? Did any of your trees show truffle presence?

[@hazeljamieson2481]

Would love an update 😊

[@godmodecrypto]

did it work?

[@awesome123103]

Any update on this project?

[@jackthemuss2493]

Cool idea. Let's hope hey

[@GriffenNaif]

You must update! Spend the money on test it's one of your most viewed. Even to show pictures of how they are growing. How you mulched? Them etc.

[@kirstyblack3432]

2:22 A PH above 7 is Alkaline. PH below 7 is an acid.

[@OakandSpore]

Yes I am aware of that. Soil is often 5-6 ph, so you use dolimitic lime to raise the ph to over 7.

[@GrosuGeoapiculturacunatura]

How did you get the spores of truffle?

[@OakandSpore]

but a truffle is the easiest way.

[@deanbristow4596]

Any updates

[@danshults3374]

Any updates?

[@backwardog1]

Different person same question. Pt.2?

[@pandaEMOx]

is there a part 2?

[@OakandSpore]

There will be. The seedlings are small still, I will get the checked shortly.

[@madaxe79]

Hope this works for you. There’s not many things that smell and taste better than truffle. I live in tropical North Queensland, so we can really grow truffle due to not really getting winter...

[@lorddrew599]

you probably wont find black truffle if you dont get cold winters . but if you get Hot summers and warm springs . You can get white truffle and what I call panda truffle that's black on outside while white with black veins through out . Try training a pig and go hunting you never know what you find

[@ssingh1772]

That's because this is all labwork...check out Micologia Forestal &Aplicada in Italy or trufficulture nurseries in Australia.

[@garyssimo]

I think he means quite alkaline, not acidic. 7 is neutral less is acidic, and higher number alkaline. I wonder if Coastal Llive Oaks would work? death caps love to grow under them.and turkey tails on dead branches.

[@jamestomlin5525]

Any updates(

[@gabewalker9170]

Lordish behaviour right there lad

[@OakandSpore]

Lordish?

[@vicekwad8537]

too cold for crocs?

[@OakandSpore]

To cold, to wet...

[@magdalenaluther6433]

Hello

[@mhmr1000]

Good morning sir what's your name please? I'm need The method of producing truffles frame start from spores please Thank you veary much sir Your friend engneer agrecutural Mhammad habeb from Syria alepo

[@BaltimoresBerzerker]

Love the idea, but if they grow, don't expect the same quality of flavor. If I remember correctly, the European truffle is superior to say Chinese truffles due to the soil they grow in

[@OakandSpore]

Considering the tress take 5-8 years to even produce a truffle, I would be happy with just a truffle, the flavor profile is at the bottom of the list! New Zealand is actually a pretty good spot for truffle growing anyway..

[@scroogemcduck1462]

"European truffles" is a different genus from the "Chinese truffles", that's why they are inferior in quality. There's nothing special about European soil.

[@BaltimoresBerzerker]

@@scroogemcduck1462 I figured as much, but there's something about the soil though. Chinese companies have tried to replicate the European quality and can't. So clearly something more is at play than variety. From what I've watched and read, people involved in the business claim it's the soil.

[@katclark1135]

Chinese truffles are raked also and so non-ripe truffles are gathered which have a VERY inferior flavor.

[@michaeladamstone2911]

It's been a year let's see what you got....

[@OakandSpore]

The guy rekons 2 years before you even check them....

[@jackthemuss2493]

First