MCAT Biochemistry: How to Solve SDS-PAGE MCAT Problems

[@raulsankhe9746]

I rarely comment on videos, but you explained SDS-PAGE so so perfectly! Thank you so much!

[@bremmethod]

I'm so glad it helped - these biotech concepts can be tricky!

[@thenightdances21]

Hi Amanda, can you please do a video on how to analyze data in the BB section? Like how to properly analyze tables, and graphs in-depth. I’m having some issues with the data & reasoning questions.

[@bremmethod]

Absolutely! I'll add it to the list of videos we plan on making. In the meantime, you can check out these two videos. The first one is an enzyme practice problem set that focuses on reading kinetic data in tables, and the second is an overview of how to approach research design and data questions which can be applied to all of the sciences! Enzyme Practice Problems: kzbin.info/www/bejne/f2qtZqiaeJWlbJo Research Design & Data Analysis on the MCAT: kzbin.info/www/bejne/g17HdYmqhrCrj6s

[@thenightdances21]

@@bremmethod Thank you SO much Amanda!! I really appreciate you adding this to your list and for both links! I’m definitely going to watch both!

[@BobSmith-yi9ti]

This is amazing, I've really struggled with these problems on Uworld and couldn't seem to understand it after reading Kaplan or watching other youtube videos. thank you so much for explaining it in such a clear manner, i'm gonna binge the rest of your videos too!!

[@bremmethod]

I'm so happy you found this useful!

[@adventurevloggeradnanahmedusa]

Got it right. Phenomenal.

[@bremmethod]

Great job!

[@amarrana2004]

yes. got it right.

[@bremmethod]

Good job!

[@Ihopeitsnottoobig]

Lmao is this O-chem? Its making some intuitive sense going through this with you. I appreciate this. Im starting chemistry over again and beginning to prepare for the MCAT. Im interested to build into this in the future.

[@bremmethod]

I'm so glad the videos are helping! Starting anything over again can be challenging, but stick with it!

[@Singercrazyforlife7]

can you help us do palindromic sequences?

[@bremmethod]

I will add it to the list!

[@LaurenC9]

Great video, thank you!

[@bremmethod]

You're welcome!

[@nmorel13]

This is SO helpful, thank you! At 9:30, how do you know that the homotrimer is held together by intermolecular forces? Couldn't it be held together by covalent bonds?

[@nmorel13]

And then following up on this, at 15:00, wouldn't the bands at 20 and 50 be twice as thick if there were supposed to be two separate proteins at each line?

[@bremmethod]

We know they are held together by IMFs because the question stem stated that there are no disulfide bonds except when unless they specifically tell us. The only covalent bonds in tertiary protein structure are disulfide bonds, so the trimer must be held together by IMFs, since that's our only other option if disulfide bonds are not present! In SDS-PAGE protein analysis, the only covalent bond interactions between monomers will be disulfide bonds - otherwise we can assume IMFs, which will be disrupted by the SDS (detergent).

[@bremmethod]

Yes - if they were using the visualization to show different band widths, we would expect to see a thicker band with multiple monomers! However, many gel figures will not show a difference in band width - this is not the actual band visual, but a rendering of it. Generally, we do not want to rely on band width unless we know that the gel is accurately representing quantity - otherwise we just have to go off of the kD sizes!

[@rajeevjoshi7564]

That’s a brilliant expression!! Thanks so much!!🎉

[@bremmethod]

I'm glad you liked it - thank you!

[@randshahrour6157]

are these problems from aamc?

[@bremmethod]

They are similar to questions you will see on AAMC practice materials and exams!

[@Mscaycay101]

Can you do a video on neurons and polarization/action potential?? If you explain it, I am certain I will understand it.

[@bremmethod]

It's on my list!!

[@dndkat]

Hi Amanda, thank you for your videos. I have 2 questions for you. Will you be covering. material from 1A? I noticed you did a few videos for 1B and 1C. Also, could you kindly make a video for each biological system (endocrine, digestive, skin, etc.)? I am aware you posted a video on kidneys. Thank you.

[@bremmethod]

I do have videos on enzymes, which is in the 1A content category! Here's the link to the content video: kzbin.info/www/bejne/b6TGlHyEZ6uJpa8si=TXCeDXwDKnP6whWF I have more videos in the works, including one on the endocrine system! I also teach all the content categories in more depth in my MCAT course, please feel free to check it out: www.bremmethod.com/bremmethodsummer2024