Protein Purification

Protein Purification aims to isolate a single type of protein from a biological tissue or culture. This video explains the most common methods applied to purify proteins, which are:
Precipitation methods, filtration methods, electrophoresis and chromatographic methods.
It is important to have an overview of these techniques in order to choose which method/methods suit the project best. Factors that can influence such a decision are: the desired level of purity, the accessibility of required equipment and the characteristics of the target protein. In practice multiple purification techniques are often combined.
Time Code:
00:00 - Intro
00:20 - Content
00:49 - What is protein purification?
02:20 - Separate your protein from a biological tissue
02:53 - Choosing the right purification techniques
03:40 - Precipitation methods
03:58 - Salting out
05:10 - Chromatography
06:08 - Affinity chromatography
07:25 - Ion Exchange Chromatography
08:07 - Hydrophobic Interaction Chromatography
09:08 - Size Exclusion Chromatography
10:20 - Filtration Methods (Dialysis)
11:12 - Electrophoresis
12:08 - Native PAGE
13:29 - Outro
At Cube Biotech we are experts in the purification of protein with over 40 years of experience. We offer a wide range of protein purification products:
cube-biotech.com/products/pro...
Furthermore, we offer custom protein purification:
cube-biotech.com/protein-serv...
For general inquiries about our company check out:
cube-biotech.com/
For more detailed information about protein purification techniques check out:
Liu, S., Li, Z., Yu, B., Wang, S., Shen, Y., & Cong, H. (2020). Recent advances on protein separation and purification methods. Advances in colloid and interface science, 284, 102254. doi.org/10.1016/j.cis.2020.10...

[@yourinfoplug8743]

The video was really useful. One of the bests I’ve watched ever on KZbin 🥂💯

[@CubeBiotech]

Thank you for the compliment! Great to hear that it was worth the effort :)

[@alhassanmohammedawal3851]

good video, it breaks the concept down to a layman's understanding. you made education interesting.

[@harryhaimoud726]

This is a master piece, believe me after a month in lab this is so accurate

[@CubeBiotech]

Thank you very much! We appreciate that the effort that went into the video is recognized!

[@harryhaimoud726]

@@CubeBiotech well done , you deserve it . I would be very happy if you do one for (Quantification of protein by Mass spectrometry) .

[@philipphanisch6840]

Such a good overview. Love the effort 🤩

[@CubeBiotech]

Thank you! That was our intention.

[@PatrickMbwire]

Well simplified. Thank you for the good work.

[@CubeBiotech]

Hi Patrick, thank you for the positive feedback! That is always appreciated :)

[@vanessaalvarez7809]

It was REALLY useful, thank you so much!

[@CubeBiotech]

Thank you! Happy that our work for that video payed off :)

[@user-pj1iv3hx1l]

This is the most useful video I ve seen on this topic so far

[@CubeBiotech]

Thank you very much. We are really glad to hear that!

[@justinsayekerwillain6908]

The video was of great help to me. Thank you

[@CubeBiotech]

Thank you! Glad that we could help!

[@meryema13]

Wowww I am beyond amazed. This lesson was explained to us in 2 hours and was so tiring and confusing but you managed to clear it all out in 13 minutes. So grateful 🙏🙏 definitely recommending this channel to my classmates❤

[@CubeBiotech]

Thank you very much for these kind words! That definetly made the day of the lead creator of this video! Thank you for further recommending us!

[@riianiu]

This video is amazing and really helpful! Thanks :)

[@CubeBiotech]

Really Glad to hear that. It makes us happy that the effort was worth it!

[@riianiu]

@@CubeBiotech 💜

[@santicruz4012]

Beautiful presentation :') thanks

[@CubeBiotech]

Glad that you liked it!

[@nicolaromanini9307]

amazing! good job! 🤩

[@CubeBiotech]

We are glad that you like it :)

[@swastiks8094]

Many many thanks for this explanation ❤

[@CubeBiotech]

We are happy it helped you. What part of the video did you find the most helpful?

[@MaryamMm-jq5np]

Very Good and useful.thanks for describing so deeply

[@CubeBiotech]

Glad that we could help :)

[@bhargaviminchu6137]

This was really helpful 🙂

[@CubeBiotech]

Thank you! Glad that you liked it.

[@userismad001]

Thanks a lot ❤

[@CubeBiotech]

You are welcome :)

[@ayhamabueisheh3772]

it is a brilliant video

[@CubeBiotech]

Thank you :) We are glad that you liked it!

[@aashiqrasool4978]

In native page , sds is not used , and proteinsc are. Not separated by charge

[@aashiqrasool4978]

Thus all don't have Negative charge

[@dr.alihosseinzadeh2643]

❤️❤️❤️

[@CubeBiotech]

We are glad that you like it!

[@mariaelkhattabi317]

Hello from California! I have a question. Do Golgi in all types of cells (liver and kidney cells) have the same function and structure ? If we were to purify the Golgi ( fractionation) from different cells, (not plant) would they function the same and have the same structure ??? I can’t seem to grasp this concept.

[@CubeBiotech]

Good Day :) We are experts on protein purification and not the Golgi-Apperatus. We do not feel comfortable to answer this for you. What we do recommend to ask this question on platforms like Researchgate. You should find your answer there.

[@mariaelkhattabi317]

Thank you so much for your guidance!!!

[@elizabethmonday3751]

Great teaching ,I enjoyed it But please ,I couldn't get a particular word you were saying at 8:34,,,, did you say soluble??? Please I'll appreciate a response,, thanks in anticipation

[@CubeBiotech]

Yes that is what was said. "The protein is just soluble."

[@elizabethmonday3751]

@@CubeBiotech thanks a bunch

[@CubeBiotech]

@@elizabethmonday3751 You are welcome :)

[@Biotechnologyguy]

❤❤😍😍😍

[@CubeBiotech]

Glad that you like it :)

[@MeghashreeGuha]

03:38 precipitation method, salting out

[@user-hk5hl6gc7y]

SDS PAGE plz

[@ebrahimalasfoor1224]

can you send me the sides

[@CubeBiotech]

There are no slides, sadly. These are, for the most part, Adobe After Effects files. :(