Microbiology: Urine Culture Primary Set-Up Inoculation and Colony Count

[@dank9261]

You are supposed to draw the loop out of the urine in a way the reduces overcharging the loop (seen as bulging of urine out of the loop) and to make sure the loop contains no bubbles before streaking and then redip the loop the same way after streaking the blood agar to inoculate the MacConkie agar. otherwise, you’ll have two extremely different volumes of urine used on the two plates. Also, avoid touching the edge of the media where it contacts the plastic plate.

[@morrisjones9537]

I was thinking the same thing as I watched her streak the plate with the same loop. Even though the loop touched a urine plate, I would have used a new loop.

[@bisterkding9249]

Even if the colony count is typically taken from the BAP, it's better practice to re-immerse your calibrated loop with every successive plate you streak.

[@erickapelaez7131]

Thank you so much for the videos! I’m glad after taking microbiology lab; it makes so much sense with the videos you are showing! I’m learning a lot and feel prepared by you since this is what my future consists of in this field!!

[@MedicalLabLadyGill]

So glad you're joining the field! Welcome!

[@MWOA_]

never use a calibrated loop method before during uni (i studied microbiology but we never use human specimen) so i never knew it's existence until i work for a polytechnic of health. a student went to me and asked about how to interpret the result and there i was, never knew that something like this even exist 🤣🤣🤣 thank you for the video, this really help me visual the result

[@MedicalLabLadyGill]

Glad to help!

[@dank9261]

Draw the edge (not face) of the loop against the cup when coming out of the urine (and away from any bubbles). This helps assure correct loading and reduces bubbles in the loop. Kind of like drawing a urine dipstick along the edge of the cup to help reduce overloading the various pads.

[@iqraismail5712]

I am from pakistan and student of bs microbiology. I am too much confused but your vidoes are informative and learn lots of things from them

[@aprilsmith8924]

This is the problem with cultures. Multiple bacteria is considered to be contamination. Please teach your students the correct info - multi species infections are very much NOT contamination and labeling it as that on cultures prevents women from getting proper treatment. Luckily, I found a urologist who gets this (very few do) and finally identified the 3 bacteria in my infection using a much better test called Pathnostics.

[@pushpendratiwarivlogs203]

How r u please I face klebssiella penumonia bacteria what I do

[@aprilsmith8924]

@@pushpendratiwarivlogs203 Please find a good doctor that will treat you with at least 14 days of antibiotics targeted to your infection.

[@pushpendratiwarivlogs203]

@@aprilsmith8924 yes bro I treat this problem antibiotics. Thanks for suggesting

[@pushpendratiwarivlogs203]

@@aprilsmith8924 Bhai antibiotic injection bhi lagane honge 5 days

[@user-fn5gr1re4w]

So true. I've been suffering for 7+years with monthly UTI'S 😫 and I didnt know I was and it was so painful and hard to urinate and bleeding. I have to take meds every month for my UTI'S. One day I had one so bad and I was starting to bleed and had to see a different doctor and they had marked my specimen as contaminated and I was like no that can not be because I am actually bleeding and the pain is horrible and I have them every month. They re did the urine culture and this time it wasn't marked contaminated so they gave me meds

[@DarkSamael55]

I love your streaking skills!

[@irfanullah-uk3ks]

Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,

[@ericyother1512]

Happy Lab Week! Thanks for the video!

[@healthknowledgeexplorer]

Thank you so much for this educative video Ma'am

[@mirzasikandar1680]

Why u can't Streak on CLED agar bc I always streak urine on CLED only.....when in student life

[@mysteryj7590]

I love the Microbiology videos!!! I'm definitely going to recommend these for my new techs. Will you please do one on gram stain interpretation?

[@MedicalLabLadyGill]

Thank you for your support! We are not in Micro this semester but I will do my best to get one done when we are.

[@sushilbarua8862]

@@MedicalLabLadyGill hello how are you?

[@sushilbarua8862]

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[@sushilbarua8862]

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[@sushilbarua8862]

Tobacco leaf disease

[@fgzy123]

Aren't you supposed to avoid spreading the specimen to the outer perimeter? Your loop keeps touching the plastic perimeter. The outer perimeter is where airborne contaminants is most likely to be located.

[@lachlank.8270]

Not worried about Proteus swarming all over the BA?

[@user-wk8ft7fr1u]

which practical microbiology book will you recommend me to read please? I highly appreciate your valuable video.

[@Gri-n9l]

We work entirely different. How do you find " clean" colonies when you are striking like this? We do 3x striking with 3 Loops and we use the same loop for the first striking on Mc and blood with no problem. How many different bacteria do you test? When you have 3 ( prxx, ec and Enterococcus for example in same count) do you test or is it contamination? Nice video!!!

[@basher94s]

Yeah..but she could have perform sub culture from this one

[@Sam-rj6kd]

im really thankful to you for making those videos 🙏🏻. i only have one question. im really confused on which type of media we use for each sample (like for urine, we use mac and blood and stool we use selenite... etc). do u have any guide/book that can help me memorize which media to use for each sample? i would appreciate it

[@MedicalLabLadyGill]

Thank you for your support! Not currently. I do have many projects in the works, this being one of them. The main thing to remember is that you're using media that will isolate common pathogens from that body site (specimen). Blood Agar Plate should grow most aerobes and facultative anaerobes. Chocolate will grow fastidious bacteria, such as Haemophilus and Neisseria, but most Haemophilus only grow on CHOC. MacConkey will grow the Gram Negative bacilli such as the Family Enterobacteriaecae (from the GI tract) and Non-fermenters (that get mistaken for Enterobacteriaecae). The anaerobic set-up would be used anytime anaerobes are expected to be the pathogen (aspirates) and should be plated along with the full aerobic primary set-up (BAP, CHOC, MAC).

[@leggeisreal1553]

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[@leggeisreal1553]

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[@mayzingasmr3145]

Very informative videos. Keep doing this educational videos. Love them. Thank you very much 💖

[@MedicalLabLadyGill]

Thank you so much for your support! I'm glad you find them helpful! ♡

[@Struggle..always]

Good work thanks ❤❤

[@RanaRana-qg5kt]

Why you putting in CO2 incubator

[@funny11744]

What is the composition of the media for this test ? Agar and what other components ? thanks

[@lelavet6829]

Thank you so much I learn a lot from your videos 🙌

[@MedicalLabLadyGill]

You're so welcome! Thank you for your support!

[@theannyoonwee8550]

Thanks you for sharing

[@sunayanastha6907]

What is this streaking technique called?

[@creativeman17]

Inoculation of agar plates to identify organisms (bacteria) present in the urine sample.

[@MarysZenDoodleArt]

Thank you 😊

[@MedicalLabLadyGill]

Thank you for your support!

[@ashankalad538]

How much temprature of incubator??

[@MedicalLabLadyGill]

Incubator is at body temperature (37°C)

[@MuruganSp-u1w]

Gnr means

[@sadikapatel2802]

Antimicrobial substances present in microbiology report meaning

[@rddtdx444444ex]

What if you test negative for leukocytes and nitrites but bacteria was present and the urine culture was from 25-50000 Cfu/ml of E. coli. Im a male

[@starparik]

Should co-relate with symptoms.

[@TheAcvivek]

is the urine sample you used diluted or undergone any form of pre culture preparation? Thank you for explaining!

[@MedicalLabLadyGill]

Good question! Urine should not be diluted or treated in any way before culture.

[@mirachaib3633]

Thanks ♡♡

[@MusicmeetsTravel]

Explained very well

[@sharnivinson7961]

knowledge you share are a true blessing Doc Kham on KZbin .

[@isaacchulu8120]

Am so helped,, am looking for someone to help me with an Incubator

[@SHIVAMKUMAR-fx5gr]

Can you make this video in hindi

[@southpawl1]

Great video

[@MedicalLabLadyGill]

Thank you for your support!

[@KrishnaKUMARMaurya-ze1kk]

What should i do

[@saeednashad4660]

wow very informative . love from pakistan

[@MedicalLabLadyGill]

Thank you for your support!

[@user-zh8yo3cy3w]

One question from my side

[@videoerxxx1230]

What does GNR mean?

[@MedicalLabLadyGill]

Gram Negative Rod (also known as bacilli)

[@chithravivek472]

Is there any vaccancy for microbiologist

[@MedicalLabLadyGill]

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[@saiyednaved]

@@MedicalLabLadyGill position available @your lab?

[@MedicalLabLadyGill]

@@saiyednaved not currently but all our neighboring hospital labs have positions available.

[@saiyednaved]

@@MedicalLabLadyGill kindly provide adress or e mail adress so I can apply for the same.

[@KrishnaKUMARMaurya-ze1kk]

I take plenty of water in a day approx 2. 0 litter

[@mirzasikandar1680]

Mucoid colonies on top klebsiella 8:02

[@user-xh1ix1lo9f]

🥰🥰🥰🥰🥰🥰💟💗💗💗

[@dr.gaballaaziz8929]

2nd step in antibiotics

[@paarwathiraj9350]

I think it is kleb

[@KrishnaKUMARMaurya-ze1kk]

Reply me soon

[@irfanullah-uk3ks]

Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,

[@MuruganSp-u1w]

Gnr means

[@MuruganSp-u1w]

Gnr means

[@MuruganSp-u1w]

Gnr means

[@MuruganSp-u1w]

Gnr means

[@MuruganSp-u1w]

Gnr means

[@creativeman17]

Gram negative rod

[@MuruganSp-u1w]

@@creativeman17 thank you