You are supposed to draw the loop out of the urine in a way the reduces overcharging the loop (seen as bulging of urine out of the loop) and to make sure the loop contains no bubbles before streaking and then redip the loop the same way after streaking the blood agar to inoculate the MacConkie agar. otherwise, you’ll have two extremely different volumes of urine used on the two plates. Also, avoid touching the edge of the media where it contacts the plastic plate.
@morrisjones95373 ай бұрын
I was thinking the same thing as I watched her streak the plate with the same loop. Even though the loop touched a urine plate, I would have used a new loop.
@bisterkding92492 жыл бұрын
Even if the colony count is typically taken from the BAP, it's better practice to re-immerse your calibrated loop with every successive plate you streak.
@erickapelaez71312 жыл бұрын
Thank you so much for the videos! I’m glad after taking microbiology lab; it makes so much sense with the videos you are showing! I’m learning a lot and feel prepared by you since this is what my future consists of in this field!!
@MedicalLabLadyGill2 жыл бұрын
So glad you're joining the field! Welcome!
@MWOA_2 жыл бұрын
never use a calibrated loop method before during uni (i studied microbiology but we never use human specimen) so i never knew it's existence until i work for a polytechnic of health. a student went to me and asked about how to interpret the result and there i was, never knew that something like this even exist 🤣🤣🤣 thank you for the video, this really help me visual the result
@MedicalLabLadyGill2 жыл бұрын
Glad to help!
@dank92612 жыл бұрын
Draw the edge (not face) of the loop against the cup when coming out of the urine (and away from any bubbles). This helps assure correct loading and reduces bubbles in the loop. Kind of like drawing a urine dipstick along the edge of the cup to help reduce overloading the various pads.
@iqraismail57122 жыл бұрын
I am from pakistan and student of bs microbiology. I am too much confused but your vidoes are informative and learn lots of things from them
@aprilsmith89243 жыл бұрын
This is the problem with cultures. Multiple bacteria is considered to be contamination. Please teach your students the correct info - multi species infections are very much NOT contamination and labeling it as that on cultures prevents women from getting proper treatment. Luckily, I found a urologist who gets this (very few do) and finally identified the 3 bacteria in my infection using a much better test called Pathnostics.
@pushpendratiwarivlogs2032 жыл бұрын
How r u please I face klebssiella penumonia bacteria what I do
@aprilsmith89242 жыл бұрын
@@pushpendratiwarivlogs203 Please find a good doctor that will treat you with at least 14 days of antibiotics targeted to your infection.
@pushpendratiwarivlogs2032 жыл бұрын
@@aprilsmith8924 yes bro I treat this problem antibiotics. Thanks for suggesting
@pushpendratiwarivlogs2032 жыл бұрын
@@aprilsmith8924 Bhai antibiotic injection bhi lagane honge 5 days
@user-fn5gr1re4w2 жыл бұрын
So true. I've been suffering for 7+years with monthly UTI'S 😫 and I didnt know I was and it was so painful and hard to urinate and bleeding. I have to take meds every month for my UTI'S. One day I had one so bad and I was starting to bleed and had to see a different doctor and they had marked my specimen as contaminated and I was like no that can not be because I am actually bleeding and the pain is horrible and I have them every month. They re did the urine culture and this time it wasn't marked contaminated so they gave me meds
@DarkSamael553 жыл бұрын
I love your streaking skills!
@irfanullah-uk3ks2 жыл бұрын
Thnkeo thnkeo ma'am,for your efforts ,,,May God bless you ,and may you get success in evey field of life ,,
@ericyother15122 жыл бұрын
Happy Lab Week! Thanks for the video!
@healthknowledgeexplorer2 жыл бұрын
Thank you so much for this educative video Ma'am
@mirzasikandar1680 Жыл бұрын
Why u can't Streak on CLED agar bc I always streak urine on CLED only.....when in student life
@mysteryj75903 жыл бұрын
I love the Microbiology videos!!! I'm definitely going to recommend these for my new techs. Will you please do one on gram stain interpretation?
@MedicalLabLadyGill3 жыл бұрын
Thank you for your support! We are not in Micro this semester but I will do my best to get one done when we are.
@sushilbarua88622 жыл бұрын
@@MedicalLabLadyGill hello how are you?
@sushilbarua88622 жыл бұрын
@@MedicalLabLadyGill see tobbaco mosaic virus leaf disease and coronavirus in microbiological microscope electronic because it
@sushilbarua88622 жыл бұрын
@@MedicalLabLadyGill search in you tube about tobbac mosaic virus leaf disease and coronavirus in
@sushilbarua88622 жыл бұрын
Tobacco leaf disease
@fgzy1237 ай бұрын
Aren't you supposed to avoid spreading the specimen to the outer perimeter? Your loop keeps touching the plastic perimeter. The outer perimeter is where airborne contaminants is most likely to be located.
@lachlank.82702 жыл бұрын
Not worried about Proteus swarming all over the BA?
@user-wk8ft7fr1u9 ай бұрын
which practical microbiology book will you recommend me to read please? I highly appreciate your valuable video.
@Gri-n9l2 жыл бұрын
We work entirely different. How do you find " clean" colonies when you are striking like this? We do 3x striking with 3 Loops and we use the same loop for the first striking on Mc and blood with no problem. How many different bacteria do you test? When you have 3 ( prxx, ec and Enterococcus for example in same count) do you test or is it contamination? Nice video!!!
@basher94s9 ай бұрын
Yeah..but she could have perform sub culture from this one
@Sam-rj6kd2 жыл бұрын
im really thankful to you for making those videos 🙏🏻. i only have one question. im really confused on which type of media we use for each sample (like for urine, we use mac and blood and stool we use selenite... etc). do u have any guide/book that can help me memorize which media to use for each sample? i would appreciate it
@MedicalLabLadyGill2 жыл бұрын
Thank you for your support! Not currently. I do have many projects in the works, this being one of them. The main thing to remember is that you're using media that will isolate common pathogens from that body site (specimen). Blood Agar Plate should grow most aerobes and facultative anaerobes. Chocolate will grow fastidious bacteria, such as Haemophilus and Neisseria, but most Haemophilus only grow on CHOC. MacConkey will grow the Gram Negative bacilli such as the Family Enterobacteriaecae (from the GI tract) and Non-fermenters (that get mistaken for Enterobacteriaecae). The anaerobic set-up would be used anytime anaerobes are expected to be the pathogen (aspirates) and should be plated along with the full aerobic primary set-up (BAP, CHOC, MAC).