Hematology: Peripheral Blood Smear & Wright Giemsa Stain

[@emjt2715]

Quick question.. my protocol says to use absolute methanol and fix for 5min… is 5min for fixing really necessary? Also, do you have to stain the cells immediately after fixing? Can I for example wait up to 1hr after fixing to stain my slide?

[@MedicalLabLadyGill]

Great questions! I would say to follow your protocol on the timing. Under fixing a slide can really be a problem. As for staining immediately after fixing, I think you can wait. If you are waiting, make sure the slides are air drying and not sitting in a solution or on a heater.

[@mayzingasmr3145]

Thank you very much . Great video 👌

[@MedicalLabLadyGill]

Thank you for your support!

[@veskinio]

What us the name of the devise you used to extract the blood from the EDTA vacutainer?

[@MedicalLabLadyGill]

DIFF Safe

[@ludwigvonmiseswasright4380]

A wooden stick works just as well. Dip the stick in the whole blood, and touch the stick to the slide, it leaves the same size drop.

[@DoloresOriordan]

Could you tell what did you use as a fixative?

[@banjo19000]

Thanks for your efforts.keep going. May God bless you.

[@MedicalLabLadyGill]

Thank you!

[@naysound4869]

Qustion what is the fixiarive u use how u preaper it ?

[@royalfolkspark]

Not on Facebook ?

[@genesisrivera1535]

Question... How much of the stain do you pour in the chamber? I'll be doing this technique in a couple of days.

[@MedicalLabLadyGill]

I pour up to the notch where the chamber widens at the top. The stain and alcohol evaporate really quickly. So, either weigh the lid down in between uses or keep refilling. Hope you have a great time!

[@genesisrivera1535]

@@MedicalLabLadyGill Thank you! Another question... what solution do you use for the fixitive sample?

[@j67d26]

@@genesisrivera1535 bump

[@blinkergurl1882]

what is the fixative?

[@MedicalLabLadyGill]

A fixative is a solution that maintains cellular morphology and prevents cellular degeneration. Some fixatives may also be used to adhere a specimen to the slide.

[@blinkergurl1882]

@@MedicalLabLadyGill did you use methanol?

[@medicalbiochemistry_]

👍👍