I would like to know what justification has to be provided to the USFDA for more bacterial count to the first 4 plates and reject them. Reason for fixing last dilutions to the fda
@afolabipeter707310 ай бұрын
Am blessed to have watch this❤
@MicroChemsExperiments9 ай бұрын
Thank you
@gousiagulzar7002 Жыл бұрын
Your videos are saviour for people like me who are not from pure microbiology field 🥹
@MicroChemsExperiments Жыл бұрын
Thank you so much.
@mukkerasreeya44579 күн бұрын
Explained so clearly
@MicroChemsExperiments9 күн бұрын
Glad to know that it was helpful
@sulemankhanvirologist5015Ай бұрын
"Excellent explanation."
@asmeretkidane4269 Жыл бұрын
very nice presentation continue with clear
@GMCLABORATORY11 ай бұрын
Why we didnt used first formula? is there any reference to take cfu between 50 to 250 cfu?
@setiawanalwisius-tu6vx Жыл бұрын
Good video, i have quation while open plate lid until the liquid agar be solid have u ever the plate countaminated, what u to do to make sure that open lid plate agar until solid could'n countaminated ?
@MicroChemsExperiments Жыл бұрын
There is no chance of becoming contaminated inside the biological safety cabinet. So, use a calibrated Biosafety Cabinet and monitor it's microbiological quality by settle plate technique periodically.
@paleshwongshrestha27982 жыл бұрын
Plz make a video of testing milk powder fat, solubility index, wettability acidity bulk density , sediment test and all ..regarding the test needed for milk powder
@MicroChemsExperiments2 жыл бұрын
Noted
@ervingopo57342 жыл бұрын
Hello, thank you for sharing such great video. May I know why plates are left opened while solidifying?
@MicroChemsExperiments2 жыл бұрын
To avoid condensation of water vapour on the lid of the dish because the media is still hot
@microbegist7918 Жыл бұрын
I find this content helpful as a student doing her project.
@zevanoc.sibarani82956 ай бұрын
Can you tell me what is the standard method you use to do this procedure? Thank you 🙏
@habibakhan35555 ай бұрын
Fda bam chapter 3 for Total aerobic count
@elinagwimile61363 ай бұрын
FDA BAM - CHAPTER 3
@bashettys7 күн бұрын
When we can use the words Absent and Less than 10 if there is no growth...and if there is no growth can we mention Zero or not...
@abhijitshukla43720 күн бұрын
Which microbes present in these PCA plates please tell me name of microbes
@mdsajibhossain7472 жыл бұрын
Same methods can we use for Drinking water or juice analysis? One more request if you possible please upload determination of yeast and Molds and Magnesium analysis in Drinking water. Thank you very much.
@MicroChemsExperiments2 жыл бұрын
Same method is for Drinking water or juice analysis. We will make video for yeast and Molds and Magnesium analysis
@KhalidSaleem-un8hd6 ай бұрын
Wow it's Amazing and helpful❤
@MicroChemsExperiments6 ай бұрын
Thank you
@SIMRANPAL-s8m10 ай бұрын
can you help me with what TPC count should be considered in acceptable range for frozen fruits and vegetables?
@officiallighthouse2 жыл бұрын
Excellent, very clear and to the point presentation.
@MicroChemsExperiments2 жыл бұрын
Glad you liked. Thanks
@nankwopmarkus4884 Жыл бұрын
Why using phosphate buffer? Can one use distilled water instead?
@SachinYadav-sk3fh2 жыл бұрын
Wow very useful video Thank you for your all the videos. Love you ❤️
@MicroChemsExperiments2 жыл бұрын
Thanks
@renutailorshorts81553 ай бұрын
Hello ma'am, this is same for anaerobic bacteria also
@ashapatil992 Жыл бұрын
Very nice procedure thank you
@MicroChemsExperiments Жыл бұрын
Thank you
@chigozieofoedu17314 ай бұрын
Nice one. Thanks
@rakibhassan24512 жыл бұрын
Your video's always awesome 😘😘😘
@MicroChemsExperiments2 жыл бұрын
Thank you
@renutailorshorts81553 ай бұрын
Please upload video for disinfectation using chlorine or which is suitable
@mahnoorsohail8479 Жыл бұрын
best video 😍
@mani-ls7kj2 жыл бұрын
Please share the determination of ammonium acetate assay method
@MicroChemsExperiments2 жыл бұрын
Noted
@balurajnadeesh27888 ай бұрын
Very useful vdo
@MicroChemsExperiments8 ай бұрын
Thank you
@hamidr_nazari2 жыл бұрын
Love you 😀🙃🙂😁😃 its so usefull
@MicroChemsExperiments2 жыл бұрын
Thanks
@adzansaban37315 ай бұрын
Sir, do you turn on the blower when working in the laminar?
@TOM-l6b3fАй бұрын
Same doubt ..do you know the answer?
@asmeretkidane4269 Жыл бұрын
how many sample is done in the same time ?
@999_finesse2 жыл бұрын
I’m stuck at a calculation. My plate count is 52 and Dilution Factor, 10^-3 Volume of sample plated is 0.1ml. My final answer is 5.2x10^4CFU/ml The accepted count is
@MicroChemsExperiments2 жыл бұрын
There is no wrong in your calculation. Your sample contains higher load of bacteria which exceeded the standard limit
@chauhanasmita46512 жыл бұрын
It's really helpful thank you
@MicroChemsExperiments2 жыл бұрын
Thank you
@FirehiwotDaselagn Жыл бұрын
nice peresentation
@MicroChemsExperiments Жыл бұрын
Thanks
@rjedey658 Жыл бұрын
Mam can I use this technique for khoaya,Chenna or any milk based sweets?
@MicroChemsExperiments Жыл бұрын
Yes you can
@neethuaneethua941 Жыл бұрын
I have a doubt regardung the buffer preparation.is the 500 ml buffer convert into 1000ml and from that only 1.25 ml is then add to 1000ml distilled water for working buffer dilution preparation?please rectify my doubt as soon as possible.
@MicroChemsExperiments Жыл бұрын
Buffer preparation is correct. Please read the reference protocol (BAM, Chapter 3) to justify it. Thanks
@neethuaneethua941 Жыл бұрын
@@MicroChemsExperimentsthank you
@neethuaneethua941 Жыл бұрын
Can you please upload a video about enrerobacteriacea (IS 17112 part ll
@MicroChemsExperiments Жыл бұрын
@@neethuaneethua941 noted
@neethuaneethua941 Жыл бұрын
I got only 4 colonies in 10^1 dilution(sum of 2 trial plates).what will be the CFU/ml?please reply mam.its very urgent for me.
@biannekaulf92212 жыл бұрын
Wow, thank you very much for sharing this.
@MicroChemsExperiments2 жыл бұрын
You are welcome
@dessienatalia11932 жыл бұрын
Is that phosphate buffer can be used as diluent solution for all products ? Including carrageenan products ?
@MicroChemsExperiments2 жыл бұрын
Yes
@gousiagulzar7002 Жыл бұрын
Can we use peptone instead of buffer you mentioned
@MicroChemsExperiments Жыл бұрын
You can use peptone salt solution
@sashikalawanasinghe10217 ай бұрын
Can this procedure use to butter sample?
@MicroChemsExperiments7 ай бұрын
No
@sashikalawanasinghe10216 ай бұрын
What is the procedure used to butter sample ? 😢
@yosam93932 жыл бұрын
sir can you make video on porosity,bulk and true density, car's and compression index for pulses and cereal determination?
@MicroChemsExperiments2 жыл бұрын
Will make leter
@ervingopo57342 жыл бұрын
Thank you for sharing such a great video. May I know why plates are left opened while solidifying? Thanks.
@MicroChemsExperiments2 жыл бұрын
To avoid condensation of water vapour on the lid of the dish because the media is still hot
@purushotamgorasiya71092 жыл бұрын
Very good and in detail explanation. Can you make same video for total coliforms. How to count and which method is highly accurate. MPN OR Plate?
@MicroChemsExperiments2 жыл бұрын
Video for coliform is already uploaded. Please find from the video list of this channel. Plating method is more accurate
@fathimasidhiky44122 жыл бұрын
very well explained
@MicroChemsExperiments2 жыл бұрын
Thank you
@yen4310 Жыл бұрын
What if my acceptable counts is in the first dilution, will the formula be C1/1xd?
@MicroChemsExperiments Жыл бұрын
Yes. Please share your final results here
@gargireghunath2114 Жыл бұрын
Is this method used for testing shrimp?
@MicroChemsExperiments Жыл бұрын
Yes, applicable
@khushiyadav6723 Жыл бұрын
Does the same method used in industries?
@MicroChemsExperiments Жыл бұрын
Yes. You can use
@karthiyayanikrishnan5675 Жыл бұрын
How can i calculate cfu if i got less than 25 colonies in 10^1 dilution?
@MicroChemsExperiments Жыл бұрын
Make report as
@deedee4527 Жыл бұрын
Thank you so much, well explained.
@MicroChemsExperiments Жыл бұрын
Thank you
@vikashganvit35482 жыл бұрын
Ma'am how to do antimicrobial activity of polymer films?
@MicroChemsExperiments2 жыл бұрын
Same as shown in the video
@vikashganvit35482 жыл бұрын
@@MicroChemsExperiments polymeric film is solid
@MicroChemsExperiments2 жыл бұрын
@@vikashganvit3548 dissolve in solvent at a certain concentration. Make a bore hole into the agar surface. Add 50 microliter of sample solution to the bore hole
@efeodjegba42157 ай бұрын
this is amazing thank you
@manjiripatwardhan3362 жыл бұрын
Can you please make video on MPN method for detection of Esheresia coli.
@MicroChemsExperiments2 жыл бұрын
Noted
@DESIVOCALTV Жыл бұрын
Why we make two/more petri plates of same dilution ?
@MicroChemsExperiments Жыл бұрын
There is a possibility of cross contamination in one plate. 2 plates from each dilution were chosen to avoid the doubt of cross contamination of the PCA plates
@ynen_4052 жыл бұрын
Can i use Nutrient agar and peptone water in cookies? Is it acceptable?
@MicroChemsExperiments2 жыл бұрын
You can use and will get results
@IzzyRoxy7 ай бұрын
Very clear explanation, really helped me alot thankssss 🥹💖
@karntokaali9778 Жыл бұрын
Can I replaced by nutrient agar media?????
@MicroChemsExperiments Жыл бұрын
You can but without any reference
@kotaspecial90472 жыл бұрын
Can you guide me how to test tri basic lead sulphate testing method
@MicroChemsExperiments2 жыл бұрын
Will upload soon
@TOM-l6b3fАй бұрын
Why can't we take the average of trial 1 and 2 and consider it as Cfu ? Do any one know??
@MicroChemsExperimentsАй бұрын
You can just average of two trials
@saurabhdeshmukh2605 Жыл бұрын
Of which sample you had calculated this cfu
@MicroChemsExperiments Жыл бұрын
Soyabean meal
@zarqaarshad4112 жыл бұрын
Plz upload video of campylobacter, staphylococcus,fecal coliform protozoa and helminths
@MicroChemsExperiments2 жыл бұрын
Noted
@venkybunny15542 жыл бұрын
would you please make a video on the hanna ph meter