Metabolite Extraction from Bacterial Culture

[@ashaguraka2916]

Why there is bead in bacterial culture. Should we need to do bead mill method for cell wall disruption before this metabolite extraction?

[@thedanlab6013]

No bead milling necessary! In fact, adding any steps in between growth and extraction will negatively impact your results - you want to extract cells in exactly (or as near as possible) the condition they were growing in. Changing the cells' environment will alter metabolites, even on the scale of seconds or minutes. The "bead" you see is just a stir bar, it's there because the bacteria I was growing tend to flocculate.

[@faithogbonna4884]

Please what type of filter paper was used for the extraction? My current research is on extraction of bacteria metabolites

[@sudanesegirl1]

How can we go about analysing is extract is it better to use lcms or GC ms if my goal is searching for a antibiotics produced by the bacterial colony

[@faithogbonna4884]

I was told Water is not good on GCMS column if the ratio is 2:2:1 how is the water removed from the extraction solvent