Microarrays vs RNA Sequencing

[@Biomeducated]

6:26 I'm confused: aren't the used images placed under the wrong technique, or was this intended to describe the strategy of complementary use of both technologies? (bringing along extra costs of course...)

[@lawrencemok8989]

Just wanted to say at the end 06:40 where you're highlighting the benefits and disadvantages of both you swapped the images around?

[@devorarossi961]

Remember that when comparing microarrays and RNA-seq you must take into account the number of reads you will perform with RNA-seq. For low abundant mRNAs in your sample where most of the interesting biology is, you are better off running microarrays than RNA-seq particularly since some arrays give you the result equivalent of 750 million read-outs and nobody does 750 millions reads when doing RNA-seq or it will be too costly to do and too much data to handle. If you want to discover new mRNAs, then sequencing is the tool.

[@martinsmarc8903]

thanks

[@GaXiTin]

I wish there were no background music.

[@thunderthunder9251]

How can we mix between them? and what are the best apps that we can use to analyze the data? Thank you

[@TAJ1974]

Please omit the music on future videos! Very distracting 😕

[@vitorsilva4793]

Pretty useful obrigado

[@MiguelHernandez-zq1tp]

Very helpful!! Thank you for sharing!

[@geoffpater]

Excellent video - thanks . Now it is three years on 1) Is the cost becoming comparable? 2) are specialised analysts still required for the RNA-Seq or is it straight-forward and 'out of the box' now?

[@ShakespeareCafe]

I was going to buy an RNA sequencing platform and then found out it costs $ 600,000

[@alyazan09]

thanks for these useful information

[@giversunlover]

Can we describe as analogy as RNA-seq is likely quantitativePCR and microarray is likely reverse-transcriptase PCR? As far as I understand, microarray is not an absolute quantitative and if you want to see the mutation, you need to rerun the analysis. Instead, deploying RNA-seq, you can get 2 in 1 package, a sophisticated parameter to be utilized.

[@udaradayarthna6379]

it is really helpful..thank you.

[@KmeleoNCL]

What does K-means clustering mean?

[@Hugh_Mungus]

0:06 TF2 teleporter sound💀💀💀💀

[@Jerrysis]

thanks, usefull one

[@ShakespeareCafe]

volume too low

[@santokhsingh1696]

Thanks 🎷🎷🔕📞📞

[@emmamalthouse3759]

L

[@earthworm-filledstomachbyc4254]

Seek Christ Jesus YHVH.