6:26 I'm confused: aren't the used images placed under the wrong technique, or was this intended to describe the strategy of complementary use of both technologies? (bringing along extra costs of course...)
@lawrencemok89899 жыл бұрын
Just wanted to say at the end 06:40 where you're highlighting the benefits and disadvantages of both you swapped the images around?
@devorarossi9618 жыл бұрын
Remember that when comparing microarrays and RNA-seq you must take into account the number of reads you will perform with RNA-seq. For low abundant mRNAs in your sample where most of the interesting biology is, you are better off running microarrays than RNA-seq particularly since some arrays give you the result equivalent of 750 million read-outs and nobody does 750 millions reads when doing RNA-seq or it will be too costly to do and too much data to handle. If you want to discover new mRNAs, then sequencing is the tool.
@martinsmarc89034 жыл бұрын
thanks
@GaXiTin4 жыл бұрын
I wish there were no background music.
@thunderthunder92512 жыл бұрын
How can we mix between them? and what are the best apps that we can use to analyze the data? Thank you
@TAJ19743 жыл бұрын
Please omit the music on future videos! Very distracting 😕
@vitorsilva4793Ай бұрын
Pretty useful obrigado
@MiguelHernandez-zq1tp9 жыл бұрын
Very helpful!! Thank you for sharing!
@geoffpater7 жыл бұрын
Excellent video - thanks . Now it is three years on 1) Is the cost becoming comparable? 2) are specialised analysts still required for the RNA-Seq or is it straight-forward and 'out of the box' now?
@ShakespeareCafe6 жыл бұрын
I was going to buy an RNA sequencing platform and then found out it costs $ 600,000
@alyazan099 жыл бұрын
thanks for these useful information
@giversunlover2 жыл бұрын
Can we describe as analogy as RNA-seq is likely quantitativePCR and microarray is likely reverse-transcriptase PCR? As far as I understand, microarray is not an absolute quantitative and if you want to see the mutation, you need to rerun the analysis. Instead, deploying RNA-seq, you can get 2 in 1 package, a sophisticated parameter to be utilized.