I cannot stress ENOUGH how helpful this was. Thank you so much
@abmgood3 жыл бұрын
Hello there, We are really glad to hear that you found the video helpful! :) Thanks so much for watching!
@Mkvine3 жыл бұрын
I struggled understanding RNA-seq but this presentation made it super helpful and easy to follow! Now I have a much better idea. Thank you!
@abmgood3 жыл бұрын
Glad you found the video helpful! :) Thanks for watching!
@soniakhan94728 ай бұрын
A brilliant, clear and comprehensive video!
@abmgood2 ай бұрын
Thank you!
@amonoracheal91184 жыл бұрын
This is the best introduction ever! thank you
@abmgood4 жыл бұрын
Thanks so much for watching! :)
@mayling10146 ай бұрын
Thank you for the wonderful visual+explanation! 15:53 May I know does the fragmentation occur before cDNA synthesis or after? I look through the protocol from Illumina they fragmented the RNA before cDNA synthesis
@studybooks33953 жыл бұрын
Thank you so much. My professor is a mess trying to explain this, and with this video I understood inmediatly.
@abmgood3 жыл бұрын
Glad to hear that you found the video helpful! :)
@Nefglarien3 жыл бұрын
Thank you very much for that material! I had to perform RNA-seq but I didn't fully understand it and I was intimidating to ask my coworkers for explanation and you sir saved me! Your explanation is so clear and without rush. Thank you!
@abmgood3 жыл бұрын
Hello there, We are really glad to hear that you found the video helpful! ;) Don't forget to check out other videos on our channel too! Thanks for watching :)
@romdhanemradnamji7943 жыл бұрын
Thank you !!!
@tseringyangzom46792 жыл бұрын
easily understandable and thank you for this this very informative video. is there any ways detect the circularRNA is QC step?
@antoniosilva-gc3pg3 жыл бұрын
I've seen these topics being explained many times but never in such a clear and direct way. Thank you!
@abmgood3 жыл бұрын
Thank you, Antonio! We are really glad that you found the video helpful ;)
@masumasultana12364 жыл бұрын
data analysis 22:00
@bamu58583 жыл бұрын
I am more of a visual leaner and this greatly helped me. Massive thanks
@abmgood3 жыл бұрын
Hello Bamu, We are so glad to hear that you found the video helpful! :) Thanks for watching!
@ramakrazavi631411 ай бұрын
Thank you so much for clarifying such a hard topic in a simple language. It was very helpful.
@abmgood11 ай бұрын
Glad it was helpful!
@ODNDWMSW-h9z2 күн бұрын
Andrew Shore
@Neeraj_91536 ай бұрын
great
@abmgood3 ай бұрын
Thanks!
@keyleanardtabonda14314 жыл бұрын
Thank you for this webinar. I have a question regarding the Quality Control before proceeding to the actual RNA seq. You have mentioned the Qubit, Bioanalyzer, and qPCR. Is it a step by step process or can use either one of this technology to assure the quality of the sample? Thank you in advance.
@abmgood4 жыл бұрын
Each of these tools have different roles where Qubit is used to determine the RNA concentration in a sample. This is followed by using an Agilent Bioanalyzer to determine the RNA Integrity Number. The Bioanalyzer has a limited detection range and the Qubit value helps to determine if dilution is necessary prior to running on the Bioanalyzer. If your RNA concentration is usually within range, then the Qubit assay can be skipped. Once a library is prepped, library QC will also be performed using the Agilent Bioanalyzer to determine the library size and purity, and it also provides a Molarity value, though it is not always accurate. If no other downstream quantification method is available, this molarity value can be used for loading onto the sequencer. However, qPCR is highly recommended and is used to precisely quantify the library prior to loading the library onto the sequencer. Overloading or underloading can lead to failures in sequencing or poor quality data.
@keyleanardtabonda14314 жыл бұрын
@@abmgood thank you very much. New subscriber here 🙂
@azchannel40453 жыл бұрын
oke bosss ku...
@azchannel40453 жыл бұрын
theng you.....boss
@pankajchand67613 жыл бұрын
I want to know what (amplicon sequence variant) ASV data means. What do the headers ASV1, ASV2, ...etc. mean? Where can I find such explanation?
@LucyCarr-s2y20 күн бұрын
Wilford Forks
@nrey21563 жыл бұрын
The best explanation of RNA seq! Much more clear than my lectures.
@abmgood3 жыл бұрын
Thanks for watching! :) Glad you found the video helpful!
@KingsleyIkpa-agodo6 ай бұрын
This is the best I have seen so far on RNA-seq... Thank you so much, sensei.
@abmgood3 ай бұрын
Glad it was helpful!
@nayyerlatifi8812 жыл бұрын
Hi this video was useful for me.thank you i just have a question what is the replicants mean in sequencing?
@passtool Жыл бұрын
Is single cell sequencing the hottest spot in the current sequencing field?
@urstube80513 жыл бұрын
very nicely explained thanks a lot sir, but even after sign up i cant able to get ppt of this webinar please help me in this regard thank you...
@jyotisoni98142 жыл бұрын
Happy learning. Thankyou 🙌
@abmgood2 жыл бұрын
Happy to hear that you learn something from our videos! It's our pleasure!
@sagek79492 жыл бұрын
This is the best explanation on RNAseq. Subscribed and will watch more videos! Thank you very much! This is so very helpful!
@abmgood2 жыл бұрын
Hello Sage, Glad you found the video helpful! ;) Thanks for watching and stay tuned for upcoming videos!
@kiplimosimon14297 ай бұрын
Excellent work. Thanks so much for sharing.
@abmgood2 ай бұрын
No problem! Thank you for watching:D
@JosephTaylor-v6u16 күн бұрын
Bergstrom Causeway
@diegoladron31885 жыл бұрын
Great webinar, thanks a lot !! How do I identify a novel transcript? For example, how would I know I discovered a new lncRNA??
@abmgood5 жыл бұрын
Thanks for watching, Diego! To answer your question, if a transcript has no gene annotation, compared to the reference genome/transcriptome, this would likely represent a novel transcript. Keep in mind, though, that additional validation would be suggested for this.
@kamleshkantnutan19682 жыл бұрын
very nice information for bigginers.
@abmgood2 жыл бұрын
Glad you found the video helpful! Thanks for watching!
@jtmidzi14 жыл бұрын
Very helpful, thanx!!
@juanete694 ай бұрын
Very good guide.
@abmgood3 ай бұрын
We're glad to hear that!
@ChakravarthyGarlapati4 жыл бұрын
Very helpful and told the subtle differences. I have a question can we actually perform the differential expression fo the genes between the cancer samples (example- subtype or stage etc) without comparing them to control(non-cancer samples)? I appreciate your input
@abmgood4 жыл бұрын
Yes, during the bioinformatics analysis stage, you can set up your comparisons such that you are comparing cancer to cancer samples or cancer to control samples. I.e. You can choose which set of samples to use for the comparison.
@ChakravarthyGarlapati4 жыл бұрын
@@abmgood sorry I didn't clearly mention the question in my previous message. To rid off the somatic mutations we use the non cancerous sample control. But can we do it with out performing this as do the differential analysis of cancer to cancer?
@km20524 жыл бұрын
THX
@abmgood4 жыл бұрын
You're welcome! Thanks for watching.
@biotechnologybasics60024 жыл бұрын
Excellent explanation
@abmgood4 жыл бұрын
Thanks so much! Glad you found the video helpful.
@nobodyspecial81274 жыл бұрын
prior knowledge of the gene (of course) that answers the question I had. tyvm
@abmgood4 жыл бұрын
Hi there, thanks for watching and yes do let us know if you have any questions about the material presented!
@nobodyspecial81274 жыл бұрын
@@abmgood Yes thank you very much. I am studying genomic medicine and a lot of the rocket scientist in my course are intimidating so this video is the most helpful video I have seen on rudimentary RNA-Seq and I will recommend it to co-students who are having trouble. My classes are RNA SARS Covid-19 I have a lot more confidence now.