Microcentrifuge tips and tricks

[@annavietmeier3674]

Thank you. Literally gave me the answer to what to do with my PCR tubes that need spun, when we don’t have adaptors.

[@thebumblingbiochemist]

glad I could help!

[@dhanvid1014]

Hi! This was a very helpful video, do you have any videos on how to pulse spin micro centrifuge tubes? Thank you!

[@thebumblingbiochemist]

No, sorry

[@perrywinch8628]

Hey very helpful video! I had a very basic question, so would you recommend spinning cells (PBMCs) at 3000 rcf for 1 min in the microcentrifuge ? Like is the speed ok? I am just worried because I don’t want the cells to die

[@thebumblingbiochemist]

That's probably the max you'd want to go - lower is better

[@perrywinch8628]

@@thebumblingbiochemist Omg thank you so much! I will go lower then. Thanks again!

[@thebumblingbiochemist]

Happy I could help! Maybe try 500-1000 g

[@umbnmbu4679]

How do you measure the radius? center point to which point? thanks!

[@thebumblingbiochemist]

Typically the end of the tube, but the force will vary along the tube because the radius varies (assuming tube is at an angle)

[@iphoneiphone4700]

Sorry, I meant do you measure it from the center point of the microcentrifuge to the external border of the hole where you place the eppendorf tube?

[@riazmohammad-e9f]

❤❤❤❤ very good