Determination of Acid Insoluble Ash_A Complete Procedure (AOAC 941.12 & Ph. Int. (WHO), 2019

Acid-insoluble ash consists primarily of silica and silicates. The Acid Insoluble Ash content is the proportion of a sample that is not hydrolysed by 40% HCl and is not subsequently volatilised upon the incineration of this Acid Insoluble Residue. This video represent a complete procedure for the determination of Acid Insoluble Ash of a sample.
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[@ofosuaanyarko6153]

Thanks for the video, very insightful. But please, what of the soluble ash, how is it calculated?

[@sakibhasan08]

Step -5 Here we take acid insoluble ash weigt. In this ash, filter paper ash is present. Why don’t we exclude it? If you measure in this way, the measurement can not be average?

[@MicroChemsExperiments]

We used Ashless filter paper. Ash of filter paper can be seen but the ash is without any significant weight.

[@sakibhasan08]

@@MicroChemsExperiments ohh. Thank you🥰

[@IrfanAli-xy5bf]

I am very impressed with your easy methods with calculation. So nice of you. Miss can you share the acidity of extracted fat experiment for bakery product. I shall wait for you positive response. Thanks.

[@MicroChemsExperiments]

Irfan Ali, Thank you for your comment. I can guide you to the experiment you mentioned. Videos are already uploaded. Do the following: 1. First extract the fat of bakery product following our video entitled "Determination of Crude Fat" (You can find the video of fat extraction in our playlist 'Proximate Analysis' 2. After fat extraction, determine the acid value of the extracted fat following our video entitled "Determination of Acid Value and Free Fatty Acid" (Check our video list to find the acid value) ****In this way, you can determine the acidity of extracted fat experiment for bakery product.

[@Samuraijack113]

Well explained .Can you upload video of other parameters like crude fiber, water extract in tea etc

[@MicroChemsExperiments]

Already uploded the method of crude fiber. Link: kzbin.info/www/bejne/pmLEZJ6Zqa-of9U

[@KailashKumar-wi8pf]

Total ash canten

[@arjundiwakar8491]

What is the normality use for preparing 40%solutin

[@aasifansari6346]

What acid is used dilute or concentrated

[@MicroChemsExperiments]

Everything is described in this video. Watch the video again

[@jenijeni1329]

Is this common for all food samples

[@MicroChemsExperiments]

Yes

[@JenniferOpara-c5o]

What if If I do not have an ashless filter paper close. And can I use this method for checking ash content in feed pellets?

[@MicroChemsExperiments]

Use normal filter paper and make ash of that same size of filter paper (blank) separately. Substact blank weight from sample.

[@santheepselvaraj427]

How to determinate mb value of carban?

[@Ashok-oc5xw]

Can you upload video about calcium and phosphorus analysis

[@MicroChemsExperiments]

We will make video for calcium and phosphorus analysis using AAS

[@yousraboukssessa4849]

Hello .I am looking for the purpose of this control. and on what samples on sconces. did you have the water insoluble ash method for spices. thanks you

[@MicroChemsExperiments]

This test represents the type of minerals in a sample left after dissolving in acid. We tested feed sample in this video. You can determine the Water insoluble ash by following video except you have to use hot water in place of HCl solution.

[@yousraboukssessa4849]

@@MicroChemsExperiments thank you very much i learned a lot with you

[@ushansanjula2504]

Hey shouldn't we substract the weight of the filter paper from 'w2' ?

[@MicroChemsExperiments]

No. We used an ashless filter paper. So weight of the filter paper after burning is zero

[@b_rabbitt9832]

Shouldn't the weight of filter paper be considered here, the final weight when the crucible weight is subtracted, we get the weight of acid insoluble ash+filter paper, right?

[@MicroChemsExperiments]

We used Ashless filter paper. The ash of the filter paper is weightless. Thats why we did not consider the ash of the filter paper

[@b_rabbitt9832]

@@MicroChemsExperiments gotchya👍

[@b_rabbitt9832]

@@MicroChemsExperiments how is it weightless though? The matter is still there!

[@MicroChemsExperiments]

@@b_rabbitt9832 That material has no weight after becoming Ash. Search on google by "Ashless Filter Paper" for more. You can set an experiment in your lab to search weight of Ash of that type of filter paper.

[@b_rabbitt9832]

@@MicroChemsExperiments I did and it was weightless, I will search on google though, thanks

[@dalalalka1894]

Hi, how long it takes approximately to get the result??

[@MicroChemsExperiments]

Cooling of furnace needs time. So about 26 hours needed in total.

[@ParamedicTutor]

Dear youtuber, can you plz explain me why do we need to do acid insoluble ash for foods??

[@MicroChemsExperiments]

Om Prakash, Thank you for your comment. We need to know the acid insoluble content of food to know the amount of minerals, present in a food sample, which is not hydrolysed by the acid.

[@fadumoladan]

Thank you for your experience benefit vedio

[@MicroChemsExperiments]

Thanks again

[@Wardi_lovers]

Can you upload a full video on determination of Sugar present in sample 🙏🏻❤️

[@MicroChemsExperiments]

Thanks for your comment. Noted. We will work on it soon. Stay with us.

[@sangeetakamatar6985]

Nice explanation

[@MicroChemsExperiments]

Thank you

[@paleypandu1548]

Super I'm learning and recalling

[@MicroChemsExperiments]

Thank you

[@devendranamdev4179]

Filter paper ka bhi weight aa gya hoga last me? Filter paper ka weight nhi liya tha apne phle??

[@MicroChemsExperiments]

Filter paper ash is not included because this is ashless filter paper. After making ash, the weight of filter paper will be zero.

[@devendranamdev4179]

@@MicroChemsExperiments ok thanks

[@MicroChemsExperiments]

@@devendranamdev4179 You are welcome

[@IrfanAli-xy5bf]

Hi. Hope you are doing well. I have little query regarding this test method. I have reviewed different method for the same Test. Question is there why are you ignored moisture content in this test method. I have faced incorrect results for biscuits by your method. Kindly advise me.

[@MicroChemsExperiments]

This method is developed following the AOAC method strictly. No change is done. So dont keep confusion about the method. And moisture content is different thing, why would I consider moisture in Ash testing? But if you want to test your sample on dry basis then remove the moisture content from the sample first. Then take sample for Acid Indoluble Ash test. But dont forget to mention in your report that you did the test on dry basis. Thank you.

[@IrfanAli-xy5bf]

Sir. I am not challenging to yours methods. My concern is why are they are counting moisture and show in calculation for creating confusion.

[@MicroChemsExperiments]

@@IrfanAli-xy5bf AOAC method is world's best analytical method. AOAC did not consider moisture. May I ask you, in which method you got to consider moisture content?

[@IrfanAli-xy5bf]

Sir. Actually I am lab analyst in biscuit industry, so am wanting to determine the acid insoluble ash in biscuit. Then I followed to fassi methods for cereal ( biscuits , on page no 28 ) in this method they have to calculate the moisture content in formula. Sir you can see.

[@MicroChemsExperiments]

@@IrfanAli-xy5bf Ok I got it. If they consider moisture content then their result will be on dry basis. You can also consider moisture content in the calculation then you have to mention in your test report that you have analyzed your sample on dry basis. But if you analyze following my method then you dont have to mention anything. Because In AOAC the moisture is not considered.

[@Samuraijack113]

What is it mean by acid insoluble ash? Pls give me answer

[@MicroChemsExperiments]

The ash which is not dissolve or solubilize in acid solution.

[@Vg_11849]

If Acid insoluble ash is 0.47% then total ash of flour also receiving as 0.40 to 0.50%, need clarity on it pls

[@MicroChemsExperiments]

Acid insoluble ash must be less from the total ash. Acid insoluble ash depends and varies with different quality of sample.

[@ParamedicTutor]

Please upload sucrose content and sulfur dioxide content estimation in foods like sugar, jaggery please

[@MicroChemsExperiments]

Om Prakash, Noted your asking test parameters. We will work on it soon. Stay with us.

[@sumitmishra3939]

Is this method capable of testing maida?

[@MicroChemsExperiments]

Yes

[@sumitmishra3939]

Hydrochloric acid percentage will be the same for flour testing also.?

[@MicroChemsExperiments]

@@sumitmishra3939 yes

[@MohamedIbrahim-qq5fm]

I need calcium and phosphorus analysis in feed please

[@MicroChemsExperiments]

Noted

[@biswajitdhara223]

Germ oil of maida testing video din

[@MicroChemsExperiments]

Noted

[@abuhureyraat-tijani6113]

Thanku ma'am

[@MicroChemsExperiments]

Welcome

[@JoshiNirav30]

Which type of whatman filter paper is used?

[@MicroChemsExperiments]

Joshi Nirav, Thanks for commenting here. Ashless whatman paper should be used. Full properties of the filter paper is shown in the cover of the filter paper in this video. Please take a look at it.

[@Vg_11849]

Whatman # 42 used in this test

[@sakirhossain2016]

But, filter paper's weight is mixed with the content??!

[@MicroChemsExperiments]

Samir Sakir, we used ashless filter paper. So the weight of filter paper ash is zero. Thats why no weight of filter paper will be counted in the final ash content.

[@sakirhossain2016]

@@MicroChemsExperiments Thank you,I got it❤️

[@MicroChemsExperiments]

You are most welcome. Stay with us

[@kishorbhure7582]

Thanks 👍

[@MicroChemsExperiments]

Welcome

[@anbarulhaque4720]

3.21 cfu/g is my reading .: Then how many colonies are there

[@MicroChemsExperiments]

There are about 3 colonies in 1g of your sample.

[@gerheart33]

can you use 10% HCl

[@MicroChemsExperiments]

No

[@amangul6529]

Kindly detemine procedure for crude fiber using fiber tech

[@MicroChemsExperiments]

Noted. Stay with us.

[@biswajitdhara223]

Germ oil index of maida video din

[@MicroChemsExperiments]

Noted

[@arzoosundrani1415]

Why 40% HCL used?

[@MicroChemsExperiments]

40% HCl is used to dissolve the maximum ash content in acid which is passes through the filter paper with acid solution during filtration. Ash content which does not dissolved in 40% HCl trapped and retained on filter paper which further quatified as Acid Insoluble Ash.

[@suryaprakash9249]

What is the method name?

[@MicroChemsExperiments]

Given in the Title of this video

[@ranjangiri7687]

Desiccator ????

[@MicroChemsExperiments]

Desiccator is used for cooling preventing moisture absorption.

[@rajendrarayane3248]

Please bellier turbidity tepmratur test bttt

[@MicroChemsExperiments]

Noted

[@mohammedmousa9540]

Sorry, what is the use of this analysis?

[@MicroChemsExperiments]

Please ask question related to the test/experiment, if you have. Btw you can take help from Google to find your answer. Thank you for being with us.

[@amangul6529]

Kindly detemine total dietary fiber , soluble and insoluble fibers of food like cake etc

[@MicroChemsExperiments]

Noted. Stay with us.

[@seemachaurasia555]

Hindi mai bataya karo

[@RishabhSharma-wy9qg]

what a fake accent.lol

[@MicroChemsExperiments]

If you don't like, plz skip

[@RishabhSharma-wy9qg]

@@MicroChemsExperiments 😂😂 Good work by the way. Balance मेशन 😂

[@MicroChemsExperiments]

@@RishabhSharma-wy9qg thanks for your appreciation. Plz just learn the experiment