Swab Test | Microbiological Quality of Surface | Environmental Monitoring | Surface Monitoring

Environmental monitoring involves sampling from various surfaces for microbiological quality. For example, laboratory surfaces, product contact surfaces, floors, walls, and equipment should be tested on a regular basis. Swab Technique has been commonly used for such tests. This technique is very important for microbiological laboratories and food & drug production units. This video explained the detailed procedure of swab techniques.
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Related Tag:
#swabtest
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[@suteerthpr118]

thanks a lot. all these videos were very helpful.🥰🥰

[@marinazainal4780]

Hi can you show how to do and intepret/read contact plates for environmental surface sampling? Thanks

[@SnoooooowMan]

Is that right (9x10x10)/100cm2 Cause you have diluted twice

[@ramkrishansharma6717]

Thanks sir please share more video microbiological testing releted food product dairy

[@shumaylakhan1220]

Plz make an video on salmonella, shegilla and s. aureus species testing by swab method

[@MicroChemsExperiments]

Noted.

[@Kucingcomel-1]

Hi, may I know what material did you use to make the frame?

[@muhammedvahisak1481]

can we use peptone water instead of saline water

[@wangyijee]

Thanks for the video! May I know is there ant reason for using 2 cotton stick to swab 2 directions ? Can I use the same stick for both direction. And, do you know what type of bacterial is on the plate? I often saw similar bacterial colony on my agar plate but I am not sure what type of bacterial it is.

[@MicroChemsExperiments]

Yes. You can use same stick. Type of bacteria depends what we used for the broth culture preparation. We must know the bacteria name and identity. If you work with unknown bacteria then you could know by the bacterial identification tests (biochemical, immunological and molecular tests).

[@tobulesu2769]

Thanks

[@taklabazar]

I love you for your work ❤❤

[@MicroChemsExperiments]

Thank you. Stay with us

[@TheH4T1]

Base on which u done in this procedure?

[@ruqiyakhamroon4434]

Please make a video on detection of campylobacter

[@s.priyanga9042]

9×10×0.1=9 /100= 0.09 right Can you explain ur calculation

[@MicroChemsExperiments]

Sorry, we can't explain here. We will develop a video later for chemical reactions and calculations. Stay with us.

[@hemedimshanafihiri5371]

Can I use macconkey agar?

[@bashettys]

What is procedure if swabbed area is not possible to measure...in food industry many areas where is not possible to measure by using template like tubes, bends etc....at this situation what we can do...

[@MicroChemsExperiments]

Swab the whole surface and calculate using the surface name, as CFU/tube, CFU/bends

[@ethical_trading0111]

I am Microbiologist help full video

[@sreenidhimp8625]

Thanks you

[@MicroChemsExperiments]

You are welcome

[@uzzalsinha]

Please, create a video on Shigella species detection.

[@MicroChemsExperiments]

Noted

[@younusalsqaf-oy8ix]

It's error in calculation How 9×10×0.1 =

[@ruqiyakhamroon4434]

For hand swab same procedure shld followed??

[@MicroChemsExperiments]

Yes

[@Shutterandlights]

Do you have any article that supports this formula?

[@MicroChemsExperiments]

Please find from journal websites

[@Shutterandlights]

Could you please refer any article using same formula? @@MicroChemsExperiments

[@prasanthivipparthi102]

Based on which standard u have done this?

[@MicroChemsExperiments]

FDA

[@bujjibabupothugunta3015]

iam microbiologist please pharama company microbiology test videos

[@ruqiyakhamroon4434]

Instead of TSA can I use plate count agar

[@MicroChemsExperiments]

You can

[@cutemama5765]

Can we use PBS buffer for swab test?

[@MicroChemsExperiments]

Yes. You can use

[@andreakayep.custodio3862]

Hello, what to do if the surface i will swab is in the gym equipments. Like how many hrs it will take for the swabbed samples to tranpsort in the laboratory? Fyi i am the researcher

[@MicroChemsExperiments]

Please write us in details at [e-mail]

[@ruqiyakhamroon4434]

Can i get reference of this method please

[@MicroChemsExperiments]

Environmental Monitoring, FDA, 2004

[@tasyanurafifahsyifafitriya3144]

mam, can you create video about how to autoclaf your media ?

[@MicroChemsExperiments]

Noted

[@assyifa5033]

@@MicroChemsExperiments i can't wait this video 🤭

[@MicroChemsExperiments]

@@assyifa5033 soon

[@masilamani.nmasilamani.n913]

How to check talcum powder surface?

[@MicroChemsExperiments]

You need different mechanical device or instrument

[@masilamani.nmasilamani.n913]

@@MicroChemsExperimentsNo sir i don't have any equipments. Iam working at soap industry . so that iam asking how to check talcum powder surface using chemicals titaration method or any other options

[@harshitkumar5921]

Why saline solution is used

[@MicroChemsExperiments]

Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.

[@thikrahalaseh1841]

Can I use buffered peptone water?​@@MicroChemsExperiments

[@tasyanurafifahsyifafitriya3144]

mam, can you create video about how to make media Nutrien broth or others,please

[@MicroChemsExperiments]

Noted

[@assyifa5033]

@@MicroChemsExperiments i Will wait this video

[@wesleychebii5684]

Why sodium chloride is used ?

[@MicroChemsExperiments]

Culture suspensions are prepared in saline solution because 0.85 % sodium chloride solution is isotonic in nature. Normal saline solution is routinely used as a diluent to adjust the turbidity of bacterial cell suspensions to help maintain cell integrity and viability.

[@wesleychebii5684]

Thank you for the clarification ♥️♥️

[@MicroChemsExperiments]

@@wesleychebii5684 you are most welcome

[@s.priyanga9042]

9×10×0.1 =9 /100= 0.09 can u explain this calculation

[@Rankun8]

please describe the reference document.

[@MicroChemsExperiments]

This is a FDA method

[@user-et6sx2hb1k]

Can you told us where can find the method. I can find it. Please cited..@@MicroChemsExperiments

[@TheH4T1]

​@@MicroChemsExperimentswhat number in FDA

[@actionspeakz2513]

How could plastic vials survived in 121 degree temp. 😮

[@MicroChemsExperiments]

Those plastics are autoclavable in quality to survive 121 degree Celsius moist heat

[@SuperRajdahal]

Wrong calculation formula, 0 .1 will be on denominator, Rest video is good.,

[@MicroChemsExperiments]

Superb. You are right. We did mistake here. Sorry

[@Bavanigirl90]

Hi, so may I know what is the correct equation and right answer?

[@user-et6sx2hb1k]

@@Bavanigirl90 N= nV1/AV2

[@ramprasath6814]

The same question ❓