Streak Plate Technique for The Isolation of Pure Culture_A Complete Procedure (Microbiology)

[@lookatmenoww]

I'm a third year laboratory medicine student and I did it for the first time few days ago. It was so interesting!

[@MicroChemsExperiments]

Thanks. Stay with us

[@sumellikanxarmy786]

I am a pharmacy student in 2nd year wherein I just went to college for 1 month and again due to lockdown the college is closed but in that 1 month we did the practical of microbiology and I felt it so interesting!! Again I am watching this video for exam point of view but still it reminds me of what I had done 😄 Thank you for the video!!

[@MicroChemsExperiments]

Thank you so much

[@ishmaljamil418]

Thanks sir I am studying Microbiology , it helped me alot.

[@MicroChemsExperiments]

You are welcome. Stay with us

[@Praisny]

Mee tooo 🙂🙂

[@Jowzeywithhealth]

I'm a first year student persuading Environmental health science... this video helped me to tackle my microbiology practical exam at part of fecal contamination

[@melissavelez5875]

I like the video, but I think to wait 5 minutes is a lot of time if you need process many samples. You could cool the loop in the agar.

[@MicroChemsExperiments]

Yes. You are correct. You don't need to wait for 5 min. Usually loop takes to cool max 1.5 min. You can cool faster by touching/stubbing on the sterile agar surface.

[@melv1n_official]

In my workplace, we use disposable inoculating loop.

[@MicroChemsExperiments]

@@melv1n_official No problem. But it must be sterile

[@amnamalik3294]

@@melv1n_official same here

[@karina4144]

Or you can just have extra loops not just one.

[@sonandpets1215]

MicroChem's Experiments team, YOU ARE ABSOLUTELY AMAZING!!! Thank you so much for sharing!

[@MicroChemsExperiments]

Thanks a bunch.

[@mikhaatef2939]

Back to my first studying year, I was doing it with less efficiency but know thanks to god I do it with more efficiency with a single burn and my prof learnt us to cool the needle in an empty area in the plate so it is a less time consumer to strike a plate and so interesting !

[@Rajput_ff001]

I am a Hindi board student but I understood this video very well because of easy explanation. Microchem's experiment team your videos is amazing! Thank you so much😍😍😍

[@swarajboddu7876]

Thank you very much for your briefly explanation it is very useful for my tomorrow practical examination

[@MicroChemsExperiments]

You are most welcome. Stay with us.

[@mashachan2437]

I’m a first year dietitian student and I don’t know why are we studying microbiology. But this video was so informative

[@MicroChemsExperiments]

Microbiology is very interesting. Anyways, thank you

[@dimadzongkha]

Food science is strongly related to food microbiology. Knowing at least a little bit is essential

[@MicroChemsExperiments]

@@dimadzongkha You are right

[@sahinasapnam1704]

Microbiology is very essential to know about microbes as u know that microbes causes dental plaque and all oral oriented infection🤩🤩 I am microbiology student

[@MicroChemsExperiments]

@@sahinasapnam1704 thanks for staying with us.

[@sivaramakrishnaprasad1777]

Thank you very much for your clear explanation.

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@haiama3rof411]

I would recommend writing infos on the backside of the agar plate not the the lid to avoid mixing up mistakes 🤷🏼‍♀️

[@MicroChemsExperiments]

We write info on the backside. But in this video we wrote on the lid to make it visible to you properly. Anyways, thanks for your suggestions.

[@antoine3933]

As a microbiologist it's literaly the less efficient isolating technique I've ever seen. You could sterilyse only once, and do quarter cross on plates, to isolate FCU on the petri dish, in a single burn.

[@MicroChemsExperiments]

In our lab work, we also complete streaking by burning the loop for single time. But sometimes single burning is not enough to get the isolated colony, specially for the beginner. Because they draw streaking lines roughly overlapping the lines with one another. Listen, our aim is to get isolated colonies and I can guarantee you that if you follow this method of streaking, you will must get isolated colonies on any of the streaking lines. So how can you say that it is a less efficient method where it serves our purpose? Anyways, if you are already expert in streaking, ignore this video. This video is only for the beginner. Thanks for being with us. Peace

[@antoine3933]

@@MicroChemsExperiments I guess it can be for beginners. Yes I didn't knew before watching it 😂 I always manage to get isolated colonies with single burns 😅

[@antoine3933]

@@MicroChemsExperiments but I meant less efficient because of the many burns needed. When I have to do like 300 petri in a day sometimes, if I lost 20min in loop cooling, I wouldn't be able to do a single day of job in a week 😂😂

[@MicroChemsExperiments]

@@antoine3933 You are thinking in the professional way. We also do streaking in single burn at our lab and we also know that the burning loop doesn't need 5 minutes to cool. But we made this video in this way, thinking about the beginners. Anyway, let it go. Thanks for commenting here.

[@antoine3933]

@@MicroChemsExperiments I guess haha Yes yes ofc, few seconds with some "trash" petri dishes are good too 😉 Yep didn't knew my bad, have a nice evening 😁 👋

[@microbiologywithvrunda7571]

So precisely shown. Easy to understand and follow the steps.

[@MicroChemsExperiments]

Thanks

[@akshayasubiramaniyan2351]

Thanks for this video iam frst year microbiology thank you sir🙂🙂

[@MicroChemsExperiments]

Thank you. Stay with us for more videos

[@MEGANTHANVL]

It was very useful to me to complete my project....❤❤❤

[@tanizatasnim7001]

4th year pharmacy student here, doing 2nd year microbiology lab only now thanks to the pandemic. Its microbiology lab final tomorrow, here to see why last time I didn't get any growth in my media! I probably used the loop when it needed more time to cool, the bacterial cells may have died.

[@MicroChemsExperiments]

Maybe

[@synthetickiwi]

Very helpful for my microbiology class. Also what is the background music, it's very good?

[@MicroChemsExperiments]

Thank you very much!

[@kamalkantkalia]

Loop should be sterilized by holding it vertically over the flame so the loop and entire length of the wire is red hot at the same time 👍

[@MicroChemsExperiments]

You are correct.

[@ellanilssonlore]

Thank you so much this is truly inspiring...

[@ahadalam9190]

Thank you so much...very helpful for my experiment

[@MicroChemsExperiments]

You are welcome. Stay with us.

[@keerthibooshan]

Actually you can streak the plate within 10 - 30 sec after striling the loop as it is made of metals that has the capability to cool faster.

[@Blevic18]

10-30seconds is short for streaking but cooling off a loop can be gaster than 5 minutes

[@MicroChemsExperiments]

Yes

[@muhsinamohammed]

Whether there is a need of dipping in the bacterial culture after heating the loop in between intervals of streaking.

[@bassantmohamed7436]

That's really great that's my first vid I watch on this Channel I benefited a lot but I really want to now how we could count on colony counter ?? Because I will have an practical exam about it 🙂 your new follower from Egypt 🇪🇬

[@MicroChemsExperiments]

Different colony counter have different techniques of colony counting. Follow the manufacturer's instructions of your colony counter device

[@clover2110]

I'm watching this rn bc im gonna do streaking on an agar plate later for my microbio & parasitology class 😌

[@MicroChemsExperiments]

Okay. All the best

[@duraideepa2283]

I taked microbiology department sir .This is my first year of microbiology department.

[@MicroChemsExperiments]

Great!! It is a wonderful and interesting subject. Stay with us for learning Microbiology practical.

[@Ruunako]

im a vet nursing student and im watching this as i prepare for my practical assignment xD

[@MicroChemsExperiments]

Thanks for staying with us.

[@Nanuramshrivas-yn8io]

Thank you sir I am studying microbiology students 1 year

[@MicroChemsExperiments]

Thank you. Keep watching our videos to learn practical knowledge

[@oluwanifemisamuel917]

Thanks for the video. Very explanatory

[@MicroChemsExperiments]

Thank you. Stay with us

[@swethamicro5420]

Thank you for ur clear explanation....it's very useful for me keep it up...💖

[@MicroChemsExperiments]

Thanks to you too. Stay with us

[@_N.S._909]

Very nice thks this is my topic for tomorrow 🥺

[@MicroChemsExperiments]

Most welcome 😊

[@DrAtifaAmbreen]

Very informative video Thanks for sharing

[@MicroChemsExperiments]

Thank you

[@hirakhalid6582]

1:17 from where did you get the bacterial culture? How do you made this?

[@MicroChemsExperiments]

We purchased the reference bacterial ATCC strain and from there we cultured the bacteria in a plate. You can also use any bacterial culture plate for streak plate technique.

[@sahinasapnam1704]

We isolate bacteria from various samples in our lab

[@nishantsen6820]

@@MicroChemsExperiments American type culture collection?

[@مهندصباح-ص3ن]

Thank you very nice from Iraq

[@MicroChemsExperiments]

Most welcome

[@oarisseesther2335]

Thanks for the brief explanation 😊. But sire do you have a video on stool mcs?

[@MicroChemsExperiments]

Not yet

[@matildeesposito7963]

It's my beautiful work 😁😍

[@MicroChemsExperiments]

Thanks

[@naliahmed8567]

Cool😍but its a little bit different from our work in lab for the same test

[@MicroChemsExperiments]

Okay

[@demitriwelling1348]

Excellent video.

[@MicroChemsExperiments]

Thank you so much.

[@prashantkumarbsmustudent8492]

Very Informative

[@MicroChemsExperiments]

Thank you

[@Smarteveryday0705]

Good video

[@oneshotsshorts1618]

thanks for the video, the only drawback is the annoying music

[@MicroChemsExperiments]

Ok we will cut off the music from upcoming videos

[@SalmanKhan-oe7gv]

I cant understand! What is the purpose of this zig zag streaking pattern! Can u elaborate?

[@doricenguemadje5761]

If you noticed that you have no isolated colonies on your streak plate( the streaks are all thicks) why do you think this happened? Thank you.

[@madhavikuruva2846]

Tq sir for ur clear explanation

[@MicroChemsExperiments]

You are welcome

[@ghalluhnurulmalika2218]

after we strake , it fills the petri dish, not in scratches, is that contamination? Or too much bacteria?

[@anjalisharma314]

Sir , shall we take bacteria from culture only for once time and go on streaking again and again . I mean no need to take bacteria every time we streak?

[@MicroChemsExperiments]

You are right.

[@anjalisharma314]

@@MicroChemsExperiments ok tell me one thing more I have culture a bacteria and pour into a test tube with fixed amount of cells. I want to incubate it once again by adding an inorganic compund into it. Shall into the same test tube, I should add inorganic compound and keep it in the incubator at appropriate temp overnight?

[@minhasanbar1854]

Kindly mention the name of this streaking method and also answer require why we are streak in four portion.

[@MicroChemsExperiments]

It is needed to get isolated and separated pure colony. This method is called: 4 quarter streaking

[@rosamariaapesteguiainfante9161]

Es una técnica que aísla bien las colonias, pero en laboratorio no tienes tanto tiempo para practicar esta técnica, cuando te esperan muchas muestras para procesar......

[@MicroChemsExperiments]

Please write in english

[@aaryamohite9103]

Very gooood information thank uh soo much for it ❤

[@MicroChemsExperiments]

You are welcome.

[@aaryamohite9103]

Can u make bacterial endospore staining and gram staining.. Video

[@MicroChemsExperiments]

Yes. We will make

[@aaryamohite9103]

Thank uhh soo much 😊

[@MicroChemsExperiments]

@@aaryamohite9103 you are welcome

[@Ilemaurice687]

Plz help me for this also explain how techniques in marine microbiology can be applied to the isolation of marine bioactive compounds, biomaterial production, production of pharmaceuticals, industrial processes, food production and bioremediation

[@MicroChemsExperiments]

Noted.

[@Quesofoods]

Very informative video

[@MicroChemsExperiments]

Thank you

[@zc7504]

thank you very much for the demo! Wondering how to confirm the bacteria at the species level? Do we streak colonies in a new plate based on their morphology?

[@MicroChemsExperiments]

Culture bacteria into Selective and Differential media first. Then go for the biochemical confirmation. You can also go for the serological test

[@antoine3933]

Or on molecular based

[@mohadesearabnia]

Very good

[@MicroChemsExperiments]

Thank you

[@fauziyadanladi1986]

Please how can one isolate pseudomonas aureginosa from vegetables?? Can this streak method be used? Please it's urgent and I need help for my final year thesis. I have checked everywhere on the net but nothing fruitful

[@lluisserramorales927]

It's so interesting but I don't know where the bunsen was, but if it was too far away, the handle has been contaminated again. For the rest, okey.

[@MicroChemsExperiments]

Bunsen is just beside the Biological Safety Cabinet.

[@johnbenedict8395]

Is the fourth quadrant ideal to take an isolated colony? And why so?

[@MicroChemsExperiments]

Colony density will the higher in first and second quadrant streaking line, thus no isolated colony will be found. But in the third and fourth quarter lines, colony density is much lower & you will find many isolated colonies in pure form. Colony density is decreased from first to fourth quarter lines because of the frequency of burning loop. Burning loop helps to lower the bacterial cell gradually.

[@IniubongInyang-vv4vf]

Please can you tell the colour, size and shape of the growth in the observation method

[@MicroChemsExperiments]

We will try to develop method for it later

[@sadafshakil6417]

Thank you so much

[@MicroChemsExperiments]

You are welcome

[@obaidako]

In my laboratory I used plastic sterile for rapidly work 👍👍

[@MicroChemsExperiments]

Great 👍

[@abdullahmohamed7162]

Very interesting content, really appreciate your effort, Please how can l isolate colony from double layer ager plate as VrbL, VrbG and the colony not on surface but in between the layer or inside the layer

[@MicroChemsExperiments]

Just stub into the semisolid media targeted the bacterial colony with inoculating loop. If you get confident about that the loop touched the target colony a little then take out the loop and streak on another fresh culture plate.

[@dasaripravalika4314]

TQ sir nice explanation

[@MicroChemsExperiments]

Thanks

[@swathidurgabhavanipaila4844]

Tq so much helpful video

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@فتىالمختبر]

Iam a third year medical laboratory student ( semi final ) and till now my streaking is so bad can you help me with that and tell me how I am going to solve this problem ? 😊😊

[@MicroChemsExperiments]

It's a practical issue. I can't solve it at this moment. Try to follow my video strictly

[@jfnh1957]

Existe traducción de los subtitulos en español? Gracias.

[@MicroChemsExperiments]

Puede obtener subtítulos en español desde la opción de subtítulos en el reproductor de youtube. Busque la opción de subtítulos durante la reproducción del video y luego seleccione el idioma de los subtítulos en español.

[@Ilemaurice687]

Hello Plzz i really need yur help can u plz help me in these questions Q1 Determine the value of microorganisms and describe their role in different industries and/or as pathogens 2.Explain different methods of sterilization and disinfection and their mechanism of action 3. explain how microbial diversity may be determined relate the structures and functions of marine microorganisms to their habitats 4 describe the metabolic pathways in major primary producers in the marine environment 5 identify the classes of metabolic types present in various marine habitats relate microbial processes to global processes and to climate change 6 describe the various adaptations that have evolved in marine microorganisms relate these adaptations to the microenvironment design strategies to apply such adaptations in biotechnology

[@MicroChemsExperiments]

Please find the answers with the help of Google resource

[@Ilemaurice687]

@@MicroChemsExperiments cant find answer on google

[@monicanyamu2207]

Thanks

[@nahidayashmin6084]

Can u upload glycerol stock preparation for stock pure culture bacteria. I used bile esculin agar for medium for primary and subculture.so what porportion of glycerin stock should i use?

[@MicroChemsExperiments]

Video is already uploaded

[@frankjosephtisoy3089]

Hi I just want to ask, for the contamination check of freshly made agar media- what is the time frame for incubation? and what temperature is ideal? thank u

[@MicroChemsExperiments]

35-37 degree Celsius for 18-24 hours.

[@1rrrrrrrrrrr544f]

I've never liked microbiology, and I will never do Another edit: I love microbiology, but I'm not gonna become a microbiologist ❤ Edit:Today(after waiting for 6 months) I had my first lab session, it was terrible, made me hate microbiology even more,but after reading (Diagnostic microbiology book) ,I got something and things made some more sense..

[@MicroChemsExperiments]

Opss... Okay

[@levymadrid9915]

hello po ilang minutes po yung incubation ng new culture media? at ilang degree celsius po?

[@MicroChemsExperiments]

Sorry, I am not getting your language. Plz write in english

[@andilenene2741]

Why should the inoculation loop not be toohot when taking culture?

[@MicroChemsExperiments]

Because excessive heat will kill the microorganisms in that colony which is touched by the loop, thus no pure culture will be found in subcultured plate.

[@08_qurataayundyahpalupi45]

Is it okey to open the petridish that much? I mean in what i learn we just open a half of the petridish but, in the result is still have contaminated but you have done it so widely and have single colony too amazing :"). Also, in what i learn when we burn ose, we just wait it not that longer and we burn every quadran. I was afraid that i'm doing wrong. This video is so informative. I will try it later. Thanks.

[@landsey9444]

I am not sure but I think it's okay? since she/he's working on a BSC

[@MicroChemsExperiments]

Yes. It is okay to keep the petri dish open for the long time if you work inside the Biological Safety Cabinet. Your plate will not be contaminated. Follow my video properly. You will get isolated colony.

[@FarzadSalim]

thank you

[@MicroChemsExperiments]

You are welcome

[@vaibhavisuryawanshi6163]

Thanks sir 😊

[@MicroChemsExperiments]

Thanks to you too

[@rvscreation8605]

Sir , l am in biotechnology first year and l have this practical in microbiology subject ,can you clear my doubt that T - streak , quadrant method ,hexgonal , simple streaking is these are the method or it just steps that we have to follow while performing this experiment? Please tell me sir.

[@MicroChemsExperiments]

You have study more. You are the beginner. Search these words in Google and try to understand.

[@nupurnarkar8459]

You have to take new smear eveytime you sterilize the loop right? Like after making the first streak you sterilized it and then again you took the smear from bacterial culture?

[@diyoradharmi6856]

No,only first time you need to take smear because he was performing four flame streaking method.

[@MicroChemsExperiments]

Exactly

[@angelinandriamaharo7801]

hi, I'm a fifth year in chemistry. I used TSA for soil analyze (sediment). Is there a method to read the colonie result? for example, we got yellow colony, orange colony, white colony. thank you

[@MicroChemsExperiments]

We will upload video tor Total Bacterial Count soon. Stay with us

[@angelinandriamaharo7801]

@@MicroChemsExperiments Willyou use TSA for total bacterial count?

[@MicroChemsExperiments]

@@angelinandriamaharo7801 No. I will use Plate Count Agar. But you can also use TSA.

[@aishwaryashivale9648]

What questions will the examiners ask on practical exam streak plate techniques

[@MicroChemsExperiments]

Clear the basic watching this video and take the full concept. Answering to the questions will be easier

[@venkateswaridasari323]

Goood vedio

[@MicroChemsExperiments]

Thanks

[@000nani]

thanks twin

[@MicroChemsExperiments]

You are welcome

[@kannaaentertainmentssubscr5801]

Sir I have completed bsc microbiology with biochemistry in 2003-2005 year, now is there any possibility to get job on it plz tell me sir

[@MicroChemsExperiments]

Apply in different lab

[@Iamme516]

This process seems so simple but somehow I always screw it up.

[@blackwolf7264]

Why shouldn't the loop be burned for the third and fourth quadrants??

[@MicroChemsExperiments]

Low bacterial cell for third and forth.

[@rameshkharbuja4134]

How can we test quality of media prepared specially culture plates?

[@MicroChemsExperiments]

Go for the growth promotion test using Bioball of reference culture

[@kehindeadebiyi8256]

helpful!

[@MicroChemsExperiments]

Thank you.

[@gabrielbaieel8073]

Its necessary to esterilyse the loop in between smears?

[@MicroChemsExperiments]

Yes. It is necessary to get isolated colony

[@duraideepa2283]

I think your videoes are help for me

[@MicroChemsExperiments]

Thank you

[@polrobinson1515]

Do you mean Laminer Air Flow as Biological Safety cabinet

[@MicroChemsExperiments]

Yes. You are correct

[@anjalisharma314]

tell me one thing more I have culture a bacteria and pour into a test tube with fixed amount of cells. I want to incubate it once again by adding an inorganic compund into it. Shall into the same test tube, I should add inorganic compound and keep it in the incubator at appropriate temp overnight

[@MicroChemsExperiments]

Yes. Add into the same tube

[@anjalisharma314]

@@MicroChemsExperiments Two questions: 1. Is inorganic or organic compound effect the growth of bacteria. 2. When we add organic or inorganic compound in to.i think there is need to add nutrient media also so that the bacteria can grow well.please guide me. 3. If we take cells from these tubes and put with fixed amount to other four tubes along with inorganic or organic compound. Shall we add nutrient media again Please reply me.

[@fransiskamalau8157]

Do I have to wait for 5 minutes if I use a single-use ose?

[@MicroChemsExperiments]

Don't wait for 5 min. Just wait enough to cool the loop

[@virtuality400]

how many times we need to deep the inocolum loop in the bacterial media??only once! i don’t understand this😣as you again&again burning the loop& after cooling you are doing zigzag but without deeping into the bacterial media but you did deep before zigzag at 1st attempt😣

[@MicroChemsExperiments]

We need to touch the bacterial colony only once at the very first time. For taking bacteria. Then we don't need to dip again

[@purnimapanthi5646]

How much quantity should be taken into the loop??what is the diameter of loop??

[@MicroChemsExperiments]

You can take a full of a single colony. Or you can just touch the loop on a colony.

[@worldofshubhi2918]

Sir could you please tell me if Microbiology is required for working in micro laboratory????

[@MicroChemsExperiments]

Yes, obviously. For microbiological analysis you always need to work in a clean microbiological laboratory.

[@worldofshubhi2918]

Sir I am done my bsc Microbiology, could I allgible for working in Microbiology laboratory??

[@MicroChemsExperiments]

@@worldofshubhi2918 Obviously. Why not?? Come on!! You are a Microbiologist. Apply to your suitable job. I hope you will do better in Lab work. All the best

[@worldofshubhi2918]

Thank you sir, it's helpful 😊

[@MicroChemsExperiments]

@@worldofshubhi2918 You are welcome. Stay with us.

[@sunny4483]

Why streak plate is not useful in quantitative estimation?

[@MicroChemsExperiments]

Because you can't count all of the colonies on the plate and many colonies are burnt during burning the inoculating loop

[@sunny4483]

@@MicroChemsExperiments Wooh! Thank you! That's helps a lot

[@jazzopas-iamkajorn5192]

Good morning sir, is this also applicable in isolating endophytic fungi? Thaank you sir 🙏🏽

[@MicroChemsExperiments]

Good Morning dear. Yes. You can use this method for fungus culture also.

[@jazzopas-iamkajorn5192]

@@MicroChemsExperiments Thaank you so much for your response sir, More power to you and your channel 🙏🏽❤️

[@MicroChemsExperiments]

@@jazzopas-iamkajorn5192 So glad to hear that

[@anamtahzada4442]

How much time needed to incubate for contamination test

[@MicroChemsExperiments]

18-24hours

[@minhasanbar1854]

If we are streak in 4 portion for decreasing microbial load. But why? We are already streak on specific culture medium.

[@MicroChemsExperiments]

A single bacterial colony have millions of bacterial cell. At the time of streaking, smear contains maximum cell number. First quarter portion will contain lower cell than smear. Second & third quarter portion will contain lower cell number than first quarter. The fourth quarter portion contains lowest cell number. Bacterial cell is decreased gradually from first to fourth quarter.

[@MicroChemsExperiments]

Decreasing of microbial cell is necessary to get isolated colony in most pure form

[@minhasanbar1854]

@@MicroChemsExperiments sir thank you for replying but my second question is why we are decrease colony?

[@minhasanbar1854]

@@MicroChemsExperiments thanks

[@MicroChemsExperiments]

@@minhasanbar1854 please knock in our Facebook page for more discussion

[@IboyitieMarian]

Someone should help me with the microbial load of spoilt yam flour of both dry and wet spoilt yam please

[@MicroChemsExperiments]

Email us at [e-mail]

[@shubhamsurya2030]

Why nicrome wire is used for inoculated loop

[@MicroChemsExperiments]

Easy to sterilize by burning and cools rapidly after sterilizing

[@lokeshhr8596]

Sir what is inoculum meaning

[@MicroChemsExperiments]

Bacterial culture to be inoculated