Determination of Iodine Value _A Complete Procedure (AOAC 920.159)

[@ikegoldasiregar9927]

It's amazing that this channel makes it possible to practice straight away, making it very easy for us to work in the laboratory. Thank you🙏

[@MicroChemsExperiments]

Thank you

[@OlufemiOlamoyegun]

I have learn a lot through this your channel sir Thanks for bringing value to this noble channel. I appreciate your effort.

[@MicroChemsExperiments]

Glad to hear that. Thank you

[@hassibahassani390]

I can never find someone explains better than you did . Thank you

[@MicroChemsExperiments]

Hassiba Hassani, Awww, How sweet. Thank you so much for your comment. Be with us. ^_^

[@adissugetahun1829]

I really appreciate your effort for showing as such an amazing virtual lab. All the description was exactly in touch with each and every moments of the whole experimental framework. # as a reminder, please present as how wijs reagent is prepared in lab. Thanks.

[@MicroChemsExperiments]

Okay. Thank you so much.

[@hernandorodrigues9259]

@@MicroChemsExperiments Como saber se o carvão ativado está acima de 800% de iodo

[@giaohuynh5884]

Thanks for your wonderful and passionate video. It's really useful for my work. I wonder some point that could you explain for me, please. 1. Why we certain use Carbon Tetrachloride in this experiment. Can you explain in detail the reason why you use Carbon Tetrechloride? Can I replace it by other substane? 2. I wonder if you put Potassium Iodide crystal around the stopped? Why don't we use the solution of Potassium Iodide? 3. In the phase that you pour distlled water into the flask, you titrate the flask with Sodium Thiosulfate until the yellow is lighter before you add the starch solution in the flask. So I wonder the reason why you must to titration before adding the starch solution. Is there any theory in this phase? I really want to know. I truly appriciate your support and I am looking forward to hearing from you. Many thanks

[@esraamossad3970]

This is the best channel I have ever seen, thanks for your effort ❤

[@MicroChemsExperiments]

Thank you so much. Where are you from Ma'am, may I know?

[@esraamossad3970]

@@MicroChemsExperiments Egypt

[@MicroChemsExperiments]

@@esraamossad3970 You can contact us in Facebook also. Link is given in the description section.

[@esraamossad3970]

@@MicroChemsExperiments I have already followed you on Facebook, thanks MicrChem ✌

[@MicroChemsExperiments]

@@esraamossad3970 Thank you Ma'am. Stay with us.

[@ouamroucheamel3519]

Thank you for all, I have a question, please what represent 12.69 in the formula?

[@cna80]

Great presentation! Please continue the good work. Knowledge is for sharing.

[@MicroChemsExperiments]

Thank you so much. Stay with us.

[@hotsquarrel]

How to prepare wij's solution?? 50ml iodine monocloride desolved in how many acitic acid in ml?? Please🙏 reply

[@nuralamsiddque6629]

Thanks for sharing with us your valuables information

[@MicroChemsExperiments]

You are most welcome

[@greatlearningwithgsb5377]

All tests which i need in solvent plant laboratory testing, all tests are there on your channel thank you so much

[@AbhishekSharma-mo3nb]

1. Quality testing of raw as well as finished products of confectionary. 2. Preparation of: 1. candies, 2. Caramel, 3. toffees. 3. Collection of various types of confectionary packages. 4. Determination of sugar in confectionary product by saccharometer. 5. Determination of refractive index of sugar - solutions of different consistencies

[@AbhishekSharma-mo3nb]

Pls make vedios on this tests

[@MicroChemsExperiments]

Okay.

[@AbhishekSharma-mo3nb]

Thank you so much

[@MicroChemsExperiments]

@@AbhishekSharma-mo3nb you are most welcome

[@mimosaya4462]

Thank you for this vid. I just want to know, how do you handle these chemicals as waste after the analysis?

[@MicroChemsExperiments]

We have third party company to handle this

[@yue00]

Hi love the video！Just wanted to ask why put the flasks in dark for 30 min？will the time of this have an effect on the titration?

[@MicroChemsExperiments]

This is done to allow reactions of some light sensitive chemicals.

[@pedromiguelestevezdeleon6272]

Hi MicroChem´s, can i make this determination of iodine value in activated carbon??

[@plutoisnotaplanetbutadwarf]

Hi, thanks for making this video! It is really helpful. I have been doing my experiment about how the number of times heating the corn oil till boiling will affect the oil’s iodine value and I realized that as I did titration, there was a layer of oil-like solution at the bottom of the flask. Do you know anything about this and why does it happen? Because it has became really obvious and disturbing since the oil darkened as the heating number increased.

[@Titi-je3ff]

Before do any lab experiment i search it your channell.it is very usefull to me. Though i get wuections😉😊😊😊😊

[@MicroChemsExperiments]

Kasthuri Kk, It's our pleasure ^_^ We are planning to make videos for almost every laboratory tests. Just be with us.

[@rahmatullasaikalgar-zd8ou]

Nice good explanation,i have one question,Why we kept sample in a dark place for exact 30 minutes.

[@MicroChemsExperiments]

To allow the chemical reaction satisfactorily that could be halted in sunlight

[@SURESHKANTIWAL]

I watch iodine value analysis video many time.which is very good & clear.but i want to know without putting iv flash in dark test can be done?

[@MicroChemsExperiments]

Putting iv flash in dark is necessary

[@beverlytompkins3017]

hi , I enjoyed the video but shouldn't the sample amount been pipette out instead of poured in because the majority of the sample was on the side of the flask not at the bottom

[@asthajain4574]

You are just superb!! Can you plz make a video on sugar determination from carbohydrates...i mean fehling solns..at which point we can consider the end of the titration.

[@MicroChemsExperiments]

Astha Jain, Thank you. We have already started making video for Sugar determination. Stay with us.

[@zekariaslibena5950]

it's good job.thank you very much

[@lakshmanubodireddy2763]

Can you share determintion of startch acid hydrogen method

[@Titi-je3ff]

Who the hell disliked. Idiots...perfect demonstration. Pls low noice back ground sound adjust. We lv your channel.thanks. plc to hplc aflatoxin analysis

[@MicroChemsExperiments]

@Kasthuri Kk, Ha ha ha...1 dislike among 89 like is negligible. They have rights to express their opinion. So plz don't argue with them who disliked. What you can do, you can love the channel more & more. We will make the background sound lower from the next video. Which aflatoxin (among B1, B2, M1,M2,G1or G2) do you want video for?

[@Titi-je3ff]

B1,B2,G1,G2 now im currently initiatimg in my lab sri lanka.if you can pls do other also.it will be really usefull.u guys are amizing.

[@MicroChemsExperiments]

@@Titi-je3ff Okay. We will make it as early as possible. Thank you for your comment.

[@arefinkhanom9157]

Thank you so much for your important video❤❤❤

[@MicroChemsExperiments]

It's my pleasure

[@rickpalmer3041]

Hi. Why did you not add 10-15% KI b4 stopping with water? Thank you!

[@newsofislam1284]

You can add 10% KI solution 15 ml into flask,

[@نور-ذ5ع9و]

It was great work. I have a question? Will there be any problem if I use saturated KI solutions?

[@MicroChemsExperiments]

You can use saturated KI solution

[@نور-ذ5ع9و]

@@MicroChemsExperiments ♥️♥️♥️

[@نور-ذ5ع9و]

@@MicroChemsExperiments Can you give me your number, please?

[@MicroChemsExperiments]

@@نور-ذ5ع9و knock in our Facebook page. Link is given in the description section

[@reformlabs8742]

Very useful information, however one issue tho, you do not heat a Volumetric Flask!

[@MicroChemsExperiments]

Noted

[@Kindly_Her]

Can please ask why are we still multiplying with normality if we have converted to mass already?

[@nezzariazzeddine2509]

Can you guide me how to calculate ! Especially the blank thinks

[@brianshim4961]

Hello, may i ask what were the molarity of the Wijs solution and carbon tetrachloride used here? I would like to see how i can change the calculations if i am using different concentrations

[@MicroChemsExperiments]

Wijs (iodine monochloride) solution: 0.1 mol/L Carbon Tetrachloride: 99%

[@IvyLin-is8bi]

Hi, thank you for the video! But I have a question. What is the purpose of adding potassium iodide crystal? And can you quantify the amount of potassium iodide crystal added around the surface of the stopper? I'm a student planning to do this experiment in schoolwork.

[@MicroChemsExperiments]

Potassium Iodide helps to release Iodine. You can add 500mg into the flask

[@IvyLin-is8bi]

@@MicroChemsExperiments Thank you!

[@malithaniman1730]

Thanks. Was helpful for exams in my masters Thanks a lot

[@MicroChemsExperiments]

Thanks

[@3DCadDesignCombo]

Informative video tutorial.

[@MicroChemsExperiments]

Thanks

[@blackbirdchannel9511]

Ma'am I have a simple question what is purpose of this test or what does tell us this test?

[@foodlaboratory8079]

Calcium magnesium phosphorus ka bi video bnao mam

[@narasimhamuppala9045]

Thanks for your knowledge sharing... please make a video on "urease activity of soya bean meal"...

[@MicroChemsExperiments]

Ok...we will do it soon. Stay with us.

[@swetarawat3624]

plz also give the reason behind adding any chemicals in the given experiment .

[@aksaniamorris3042]

This was great work. One question, wouldn't the KI crystals placed on the top interference with the reaction?

[@MicroChemsExperiments]

Aksania Morris, thank you for your comment. No, Addition of KI wouldn't interfere the reaction. In AOAC it ia said to add KI.

[@aksaniamorris3042]

@@MicroChemsExperiments ok thank you

[@MicroChemsExperiments]

@@aksaniamorris3042 Welcome. Thanks for being with us.

[@Cookie-wt4uz]

Actually the KI helps to liberate the unreacted I from Wij's solution

[@newsofislam1284]

@@MicroChemsExperiments You can add 10% KI solution 15 ml into flask,

[@haceroo]

Iodine molecular weight is 126,90477 g/mol . Why was this (12,69 g/mol ) written in the formula?

[@amanueldaniel5342]

Thank you for sharing. may ask question why add KI at neck of flask when during preparation of sample and blank?

[@MicroChemsExperiments]

It is said to add in AOAC.

[@umeshyadav-xj2uz]

@@MicroChemsExperiments 👍👍

[@waytohellgamers5201]

Bcoz iodine vapours doesn't go outside of iodine flask

[@newsofislam1284]

You can add 10% KI solution 15 ml into flask,

[@SohelRana-ln7bg]

Stearic acid iodine value test is same??

[@hamzamansour593]

What an excellent explanation ❤❤ thank you so much

[@MicroChemsExperiments]

Thank you

[@rajesh7999]

Its It's very good to see experiment very clearly I have thought our chemists by showing the video... many thanks... Please update USM UNSAPONIFICATION MATER ALSO

[@MicroChemsExperiments]

NUTHAKKI RAJESH, okay we will work on unsaponification mater as early as possible. Be with us.

[@rajesh7999]

@@MicroChemsExperiments thanks

[@MicroChemsExperiments]

NUTHAKKI RAJESH, This video will help you. Please take a look. kzbin.info/www/bejne/eqHKnnuuYsmfZ6M

[@kapilmaurya8262]

Blank me starting me kya liya tha befor adding ccl4

[@sonandpets1215]

Where can I buy or how can I get the AOAC manual for the different methods? I'll still keep watching you! 🙏🐾

[@MicroChemsExperiments]

You can purchase it from Amazon.

[@bishow8781]

Full meaning AOAC

[@muhammadaltaf9651]

Appreciate your effort on preparing such useful videos.

[@MicroChemsExperiments]

Thank you very much. Stay with us.

[@AbhishekSharma-mo3nb]

Thank you so much Is 12.69 molecular weight of iodine?

[@MicroChemsExperiments]

No. Molecular weight of iodine is 126.9. Its a calculation factor which was derived using the molecular weight of iodine.

[@AbhishekSharma-mo3nb]

Thank you 😊

[@MicroChemsExperiments]

@@AbhishekSharma-mo3nbMost Welcome

[@rajatsharma8736]

0.21 gm sample h to expected iv groundnut oil me kitna hona chahiye

[@nadiaqonita8210]

thank you for making a test video tutorial like this. the video is very clear and easy to understand. It's just that there are some points that explain it too quickly, so I feel that there is something missing, but it turns out that the explanation is just too fast.

[@MicroChemsExperiments]

@Nadia Qonita, You are most wecome and thanks to you for your observations. We will try to make our videos more clear.

[@rickpalmer3041]

If the blue color keeps coming back within the 30s, what could be the problem? Right after turning milky-white, the blue color returns. This happens repeatedly even after titrating more of the thiosulfate

[@AayushiSain-s3g]

Which oil u have used in this pls tell

[@yue00]

Also when I carry out the procedure, there is a purple coloured layer at the bottom of the solution in the blank and a pink coloured in the sample, do you know anything about this?

[@MicroChemsExperiments]

Nothing noticed like that. Prepare chemicals and reagents freshly and try it again

[@janamaymagpayo7419]

hello! what is the solvent used fir wij's solution? can water be used?

[@MicroChemsExperiments]

use acetic acid as solvent

[@Ghost34121]

Can you please help me with a soft copy of the method Thanks

[@lakshmanubodireddy2763]

Please share how prepare of wijs solution

[@ukuleilei4905]

1. During titration, iodine flask should've been covered with the stopper throughout in order to avoid the escape of iodine gas. 2. calculation should've shown the units also 3. It should've been 126.9 g/mol, since that is the molecular weight of iodine.

[@daniellekeller371]

Regarding 3.: Yes, but you have to divide by 10 to get the correct unit for the IV, which is g(I2)/100g(fat). It would have been obvious, if the correct units were used in the video.

[@MicroChemsExperiments]

Noted

[@HassanDanish-vh2zu]

There's a mistake in your analysis the equivalent weight of sodium thiosulfate is 248.16g so if we want to make 0.1 N Sodium thiosulfate we need 24.6g not 2.48 g

[@arknowledgezone7741]

your calculation is for 1000 ml, but here 100 ml sod. Thiosulfate is being prepared by using 2.5 gm of it, which is correct.

[@HassanDanish-vh2zu]

@@arknowledgezone7741 Thank you ! for correcting me, its my bad

[@manojkumarsharma6177]

Dear sir mujhe ye nahi samajh m aaya aap ne blank m oil liya ya nahi

[@MohammedQatan-c3v]

Ihave iodine mono chloride in the labe but if iuse it it doesnot give me the good reading for the blank low readeing what can ido any way to increse the wigis solution strength

[@kavindibalasinghe8144]

Hi could you please let me know any alternative for CCL 4 ( apart from chloroform ). Thanks

[@MicroChemsExperiments]

No replacement to be known for CCl4 other than Chloroform

[@kavindibalasinghe8144]

@@MicroChemsExperiments thanks alot for the reply

[@s.m.armanhossen4478]

thank you

[@MicroChemsExperiments]

You are welcome

[@Sathish8300m]

i have prepare Wij stock solution using 50g ample in 500ml (makeup)acetic acid and for working solution i have take 80ml of stock solution and makeup with 500ml of acetic acid is this correct

[@MicroChemsExperiments]

We will make video for the presentation of wijs solution soon

[@sondesalmbrok162]

Thanks for the video, But if result 38 for olive oil and compared with reference rang and show less than from rang what writing comment?

[@MicroChemsExperiments]

The quality of that oil is not good and maybe mixed with other cheaper oil.

[@aqeelasif7032]

What is the unit.? Of IV in this calculation.?

[@nhuguyenn]

Hi, what's the concentration of wij's solution did you use?

[@MicroChemsExperiments]

1.623%

[@kinanujeremiah1823]

I like your explanation and demonstration Thanks

[@MicroChemsExperiments]

Thank you too. Stay with us.

[@leenziad5964]

Good job ❤❤ One question where did the value 12.69 come from ?

[@ashishshrivastv8375]

126.9( mol wt of I) divided by 10

[@arshyankhan2054]

​@@ashishshrivastv8375why divided by 10?

[@digitechsports6942]

Please how to prepare Ipa lactic acid solution for sv test of wheat flour.

[@MicroChemsExperiments]

Did you mean sedimentation test of wheat flour?

[@rebeccahmbithe3024]

I have been given unknown kind of fat or wax maybe but the point is this sample is in solid nature, how will I predict it's Iodine value so that I'm able to weigh the supposed weight of the sample?

[@MicroChemsExperiments]

Go for the first trial taking any amount of sample which will give you an idea about the iodine value (approximate). After getting the idea, go for the second and final trial taking sample weight according to the iodine value you got from the first trial

[@moonlight-td8ed]

can u pls tell the precautions needed??

[@MicroChemsExperiments]

Prepare chemicals freshly

[@poonamprisha1410]

At the end point pink color emulsion kya usko bhi milky bnana h

[@MicroChemsExperiments]

Milk color emulsion along with white solution will form at the end of the titration.

[@Itsmenandana808]

How much ammount of KI adding?

[@phenolicphtl5011]

My question​ is not about AOAC but about AOCS methods, that before weighting the sample, sample must adjust temperature to 68 -​71 celsius deg. I wonder what is the purpose of sample temperature​ adjusting?

[@MicroChemsExperiments]

Chemistry is all about chemical reactions. Chemical reactions takes place differently at different temperatures. So the adjustment of temperature is very crucial for any chemical analysis of sample.

[@ekingursoy1779]

Hi, I was wondering why is it so important that it should be closed immediately after pouring the wij's solution. By the way, this is a great video it has helped me so much. If you have any other papers, videos, or references about determining iodine value in oils by wij's method I would be thrilled If you could reference it. Again thank you.

[@MicroChemsExperiments]

Ekin Gursoy, Thanks for your comment. Actually, when Wij's solution is added, it reacts with Iodine present in the sample to form slightly volatile compound. If we left open then loss of iodine may occur. That's why we keep the flask closed immediately after adding carbon tetrachloride and Wij's solution. Other two references for this test are: IS-548 and FASSAI MAnual.

[@ekingursoy1779]

@@MicroChemsExperiments thank you very much

[@MicroChemsExperiments]

@@ekingursoy1779 You are most welcome.

[@khemist2631]

@@MicroChemsExperiments Great video, very interesting to watch. However, why was the iodine crystals added around the stopper after closing?

[@MicroChemsExperiments]

@@khemist2631 It is added to set a barrier for passage of gas from the flask to the outside during the 30min of the reaction time.

[@anannaone]

this was so well explained💝

[@MicroChemsExperiments]

Thank you

[@swapnilpradhan507]

Very informative video

[@MicroChemsExperiments]

Glad you think so!

[@amirazaharuddin3463]

For the blank , we should to find iodine value also ?

[@MicroChemsExperiments]

Not iodine value actually. Blank reading represents the chemical error for iodine value test

[@oshinmanchu]

Hi Mam Is it possible to analyse cholesterol content in edible oil by volumetric titrations..??? Without chromatography or spectroscopy plzz reply me mam...

[@MicroChemsExperiments]

We will upload video later.

[@Athokshaya_V]

What is filter paper number used to prepare 1 percent starch solution?

[@MicroChemsExperiments]

Number 1

[@Athokshaya_V]

@@MicroChemsExperiments can we use whatman no. 4 filter paper?

[@surendranathsingh8040]

Thanks

[@MicroChemsExperiments]

Welcome

[@azaruameer3931]

can i use these methods in my food quality lab of my food industry?

[@MicroChemsExperiments]

No. This method is only for oil sample.

[@azaruameer3931]

@@MicroChemsExperiments our industry make olive oil only

[@biancaysabelvicente215]

Hii! first of all, your videos are very helpful. But may i ask something, why is blank titration necessary in the this determination(iodine value det.)? I hope you answer me ,thank you so so much

[@MicroChemsExperiments]

Blank titration is needed to subtract reagent reading.

[@biancaysabelvicente215]

@@MicroChemsExperiments Thank youu Ma'am/Sir.

[@MicroChemsExperiments]

@@biancaysabelvicente215 You are most welcome.

[@sahilrohilla3863]

Heyyy , i can help you in your exams and assignments . I am a medical expert.

[@rohanbandechha9081]

what does the potassium iodide do, is it needed for the experiment

[@MicroChemsExperiments]

Yes. It is needed

[@sonandpets1215]

You are awesome! Thank you for sharing!

[@MicroChemsExperiments]

Thank you for supporting us.

[@poonamprisha1410]

Mam jo dark m rkhte ho vo only 30minutes which type of dark you use plzz tell

[@MicroChemsExperiments]

I used cabinet where no light can enter.

[@subhadevisubhadevi8517]

In this calculation step12.69 value where itcomes from

[@azaruameer3931]

Can you give me the "Determination of Oil Analysis" manual if I need it?

[@MicroChemsExperiments]

We do not provide any document

[@MohdUsman-tq6gk]

Why don't we add starch indicator in the beginning of titration? What only when the colour of the solution becomes lighter?

[@MicroChemsExperiments]

It is a two spet titration. More accuracy is achieved by adding starch when the colour is faded out.

[@amtknh]

i took 0.216 gm sample of groundnut oil its value came 93. isit in expected iv range or this answer is wrong

[@dilani25-gu6qj]

Why uou mention 12.69 Can You explain plz

[@anuragchoudhary7944]

Nice explained. How do we calculate iodine number for activated carbon.

[@MicroChemsExperiments]

Same calculation should be applied

[@anuragchoudhary7944]

@@MicroChemsExperiments what should the weight of active carbon for the experiment

[@MicroChemsExperiments]

@@anuragchoudhary7944 Use 5g sample.

[@anuragchoudhary7944]

@@MicroChemsExperiments thank you

[@benoysebastian811]

Please post a Video for Determination of TVBN in Dried Fish Meal sample AOAC

[@MicroChemsExperiments]

Noted

[@dixitkansagara7441]

I have 2-3 questions,how can i contact you? Plz ans..

[@MicroChemsExperiments]

Already answered. Do this test exactly as done in this video

[@AJInfoBook]

Can we use of chloroform instead of Carbon tetrachloride

[@harshitakaushik9058]

Yes, you can

[@AJInfoBook]

@@harshitakaushik9058 thanks

[@MicroChemsExperiments]

Thanks for the reply

[@user-df5is8jy6n]

what is the difference between this method and ASTM D4607-94 method? Can I use this method for activated carbon?

[@MicroChemsExperiments]

No. Our method is for foods and oils. We will prepare method for activated carbon later. Stay with us.

[@user-df5is8jy6n]

@@MicroChemsExperiments oh thank you very much, looking forward to it.

[@MicroChemsExperiments]

@@user-df5is8jy6n Welcome.

[@shibora]

Where Didi you get this 12.69

[@Babyvideos711]

If we don't know iodine value limit of any sample then how much weight should we have to take for calculating I.V.

[@MicroChemsExperiments]

You can get idea of iodine value for specific oil from google and journals.

[@amtknh]

for groundnut oil what weight we should select @@MicroChemsExperiments

[@kushagrasharma8915]

Can you please tell me the chemical equation for this reaction.

[@MicroChemsExperiments]

We will make video later for every test explaining chemical equation

[@blackwhite3463]

In here which is value of 12.69???? How u find it?m

[@MicroChemsExperiments]

It is a calculation factor given by the reference method of AOAC.