Milk Fat Determination by Gerber Method_A Complete Procedure (Gerber Method)

[@ZakRng]

So useful thanks alot✅

[@Hues_of_Hyru]

Can you please do a video of rose gottelib method of fat analysis

[@subbaraokorlimarla9228]

You have explained the method explicitly. Good keep it up.

[@MicroChemsExperiments]

Thank you. Stay with us.

[@mahsanjunaid5944]

I can miss this Practical in class but I am sure to perform it better than Others.

[@NaeemDiary]

Class liya kro, KZbin pe video dkh k degree ni milti 😂😂😂

[@batticaloacco1759]

Very useful video and thankyou😊

[@MicroChemsExperiments]

Welcome 😊

[@zamzamNamulinda]

Am so appreciative with the lecture 😀

[@MicroChemsExperiments]

Thank you

[@AbhishekSharma-mo3nb]

Hello that's really good vedio. Please make a vedios on this topics Organoleptic test of milk. Detection of common adultrants and preservatives in milk. Sampling equipment and sampling of milk.

[@MicroChemsExperiments]

Abhishek Sharma, Thank you so much. We will work on your mentioned tests very soon. Be with us.

[@anushanaidu1064]

@@MicroChemsExperiments yes these are very useful, we will be glad if you make videos on these

[@KonokRoy-y4f]

I understand KZbin better than what I understand in class

[@veerapratap4574]

Thank u so much. It's really clear and interesting

[@MicroChemsExperiments]

You are welcome. Stay with us

[@Jup111ter]

Milk fat testing may have changed in the 4 years since this video so I wanted to ask if your method had as well? I work in food science and production and noticed a huge difference between our gerber procedure and yours - mainly in how the butyrometer is filled. Typically we do 10ml sulphuric acid, followed by 1ml isoamyl OH to create a layer and prevent burning when the sample is introduced. Has it changed since this video and if not, will it given an alternative suggestion? Hope all is well! Great video :)

[@MicroChemsExperiments]

Thanks for your comment. Actually the method we showed in our video is still a valid method for the determination of milk fat. It is a validated method which gives accurate results. About the variations between our method and your's, different organizations developed this method keeping variation slightly (following Gerber Technique) and validated inhouse. We did so as per our best laboratory practices. Though variation, still you can achieve reproducible accurate results using our method. For more, please send us an email to: [e-mail]. Thank you.

[@vijender7179]

Thank you 👍

[@MicroChemsExperiments]

You are welcome

[@hindgg-sg1pv]

Thank you so much....💚

[@MicroChemsExperiments]

You are so welcome

[@Natasha.narkar]

Thank you so much...for this

[@MicroChemsExperiments]

You are welcome

[@yarzaraung-ph6re]

Thank, Could I know Sweetened condensed milk fat % method?

[@SheikhImran-m3i]

CAN YOU PLEASE TELL WHY WHY WE ADDING ALL CHEMICAL WHAT HAPPENED IF SEQUENCES CHANGED AND WHAT IS THE MECHANISMS OF GERBER METHOD

[@kienthucmoithucpham]

I would like to ask about the advantages and disadvantages of this Gerber method. Thank you

[@MicroChemsExperiments]

Take help from Google

[@amninazifa5244]

Hello there! I have a question regarding Mojonnier method instead. Once I done with the first extraction, does I need to do 2nd and 3rd extraction using the same Mojonnier flask I used in first extraction? And also, does the transparent solution need to be decant to the same evaporating dish in 1st extraction for 2nd and 3rd extraction? Hope you can help me with this, thank you!

[@MicroChemsExperiments]

Yes. 2nd extraction is needed using the same apparatus.

[@amninazifa5244]

@@MicroChemsExperiments thank you! I think I can understand it👍

[@nibindavidkizhikkarenparam3884]

hello. thank you for the vedio. can you please tell me the specification of butyrometer tube

[@MicroChemsExperiments]

Please take help from google

[@nagadiary7586]

Thank you for the very informative video. Mam I have one query, can we use alternative alcohol like ethanol or rectified spirit or does it have to be amyl alcohol? Thanks.

[@dr.jivankharel8391]

Hello microchem experiment team all the video created are awesome. I would like to see the video of determination of nitrogen free extract in near future

[@MicroChemsExperiments]

Jivan Kharel, Thank you so much. Be with us. Please clarify me, do you want a video of Free Nitrogen Determination test? From which sample??

[@dilanianuradhayapa4487]

Thank you so much ❤️

[@MicroChemsExperiments]

Thank you

[@hantan2265]

this 3.5% fat content of how many gram of sample ? how to calculate that fat content?Thx for teaching

[@HgO-hi4qy]

Thank you but you need to add milk or sample slowly and carefully

[@AbidHussain1175]

Doing well, keep it up 🧪

[@MicroChemsExperiments]

Thanks

[@sushantpatil6679]

Could you share Centrifuge machine specifications ?

[@varaprasad4798]

I have a question why only 90precente sulfuric acid is used why not HCL

[@MicroChemsExperiments]

It is said for Gerber method to avoid burning of milk fat

[@shubhammanna]

what kind of milk sample was that like toned, full cream which one ?

[@MicroChemsExperiments]

In this video, we tested full cream pasteurized milk sample. But you can test all kind of milk sample, including milk drinks, by following this method/video.

[@shubhammanna]

@@MicroChemsExperiments yes some days before we tested for amul full cream and got 3.4% it was bit doubtful that according to standards it should be 6% but now i got it that we aren't alone LOL

[@MicroChemsExperiments]

@@shubhammannaPrepare Gerber H2SO4 properly. If you dont get the expected result, increase heating & centrifugation time to 10 min instead of 5. You can also use 2ml Iso amyl alcohol instead of 1, which will facilitate fat extraction.

[@shubhammanna]

@@MicroChemsExperiments oh ok will do thanks for the tip ☺️

[@MicroChemsExperiments]

@@shubhammanna All the best. Let us know the result

[@rakeshkumar-vo3lw]

If we take 90mlcon.H2SO4 and add distilled water make up100ml slowly than any kind of change in results.. I request that you please tell all reaction, mechanism, role, keys point apply or for used chemical that affect your results.. for saponification value, iodine value, and acid value,etc.. thanks 🙏🙏

[@MicroChemsExperiments]

You should not add water to acid. Always add acid in water to make solution. Okay we will work on mechanism of action of every test.

[@asneelasheshlal6485]

Hello! very informative video. Requesting if you could upload videos on different types of chemical analysis of animal feeds.

[@MicroChemsExperiments]

What type of chemical analysis? Please mention the exact test parameters.

[@asneelasheshlal6485]

@@MicroChemsExperiments total digestible nutrient(TDN)

[@ربي-ارحمني]

Asque il ya d'autre méthode pour la détermination des lipides pour un animal marin

[@MicroChemsExperiments]

Yes. We have other method. We will upload video for that in the future. Stay with us.

[@ربي-ارحمني]

@@MicroChemsExperiments I hop thank you

[@jaideepkalinga7364]

Thanks

[@MicroChemsExperiments]

You are welcome

[@hajarzakzouk7458]

Hello again! Good explain as usual microchem. I have another question if you please, this method can work with plant-based milk like soybean milk !!

[@MicroChemsExperiments]

Never tried but you can try

[@akashpawar9542]

Thanks Ma'am

[@MicroChemsExperiments]

You are most welcome

[@hajarzakzouk7458]

hello microchem ! .. thanks for your helpful videos .. i want to know that, why you making heat water bath before and after centrifuge ??

[@MicroChemsExperiments]

1. Applying heat before centrifuge: helps to burn the sample in H2SO4 properly and react with Iso amyl alcohol. 2. Applying heat after celtrifuge: helps to melt & float all of the the fat content on the measuring scale of the butyrometer and facilitate to seperate the fat from the burnt sample.

[@abhayram-q6c]

Thank you mam

[@MicroChemsExperiments]

@@abhayram-q6c Its my pleasure

[@manuthapa3149]

Very complex . simply in the market we can get 90% sulphuric acid . Procedure is first take sulphuric acid , milk and amyl alcohol and shake it than put on gerber. No need to put into hotbath

[@MicroChemsExperiments]

If you are confident, go for it

[@haiderkhan3062]

Why we use H2SO4 and alcohol use in milk testing?

[@MicroChemsExperiments]

Haider Khan, Thank you for your question. Sulfuric acid is very helpful to burn and digest any sample. In this test, H2SO4 helps in acidic burning of milk sample and Iso-amyl alcohol helps in effective separation of milk fat from other content of milk.

[@beautyobsessedgal]

Does gerber really need 90% h2s04? Or can the 98% h2s04 work as well?

[@MicroChemsExperiments]

Use 90%

[@sathhinakerikanti1639]

90 % h2so4

[@ibrahimmohammed9100]

If I determine fat content of ice cream i used same method ?

[@MicroChemsExperiments]

You can use

[@viper0716]

Can you show how to test the fat content of butter using gerber

[@MicroChemsExperiments]

You can take 5gm butter into the butyrometer and do the rest of the things as shown in this video.

[@abhishekbhope8006]

our butyrometer volume is 11 ml. so how we are adding 10 milk + 10 h2so4 + 1 ml alco .total =21 ml ?

[@MicroChemsExperiments]

Solution: 5 milk + 5 h2so4 + 1 ml alco .total =11 ml

[@nada_yarniverse]

how to clean tube after using it?

[@MicroChemsExperiments]

Fill the tube with concentrated H2SO4 and stand for two hours. Then wash with tap water.

[@abhayram-q6c]

Hello madam , why should I delute the acid , what if I use 100% concentrated acid...?🤔

[@MicroChemsExperiments]

You have to prepare the Gerber H2SO4 properly as described in the Gerber's official method and also showed in this video. Gerber developed this method with 90% H2SO4. 100% H2SO4 may lead to loss of milk fat.

[@knigh_tingale289]

Can we check with boiled cow milk

[@MicroChemsExperiments]

Yes. You can

[@knigh_tingale289]

@@MicroChemsExperiments I have checked with both boiled & raw milk today.. boiled milk shows 30 CLR, raw milk shows 26 CLR.. why the difference is

[@adlilwafi8849]

Hi, I want to ask, if I were to do this experiment by manipulating the time of milk being boiled/heated before conducting the experiment to see if it affects the fat quantity, is it possible? and if so, can you suggest the heating procedures?

[@MicroChemsExperiments]

Heat the milk sample in microwave oven for different time duration and conduct the experiment to know the difference in milk fat value.

[@adlilwafi8849]

@@MicroChemsExperiments should i add in the sulfuric acid when the milk is hot or do i have to cool it down first

[@MicroChemsExperiments]

@@adlilwafi8849 After heat treatment, cool the sample at room temperature first. Then go for the analysis.

[@Rio_sk]

If you have a sample of about idk 250ml(milk) heat it in a waterbath for 20min (gently mix every 10mins so you don't make bubbles because they effect the end resault). After 20min put the sample in a cold water bath for 10min. Before adding the milk into the butyrometer you need to mix the sample if you have a 250ml flask just turn it 360° like 5-8 times but slowly, you don't want bubbles to form. It also helps if you put in this order: 1. 10ml 91% Acid (check so you don't have air pockets in butyrometer), 2. 10.75ml milk (add slowly against the wall of the butyrometer to prevent burning) 3. Add 1ml of alcohol. Tip: If you use a rubber cap like she does in this video make sure you twist the cap while pressing it in so you close it more tightly. The way she did it made me concerne it would bust open into her face.

[@sreethasanthamma9217]

is this test used for icecream

[@MicroChemsExperiments]

yes. you can test icecream by this method

[@hafezquadri2972]

I followed the same but I didn't get the results? .. Fat is not getting into the gerber scale

[@MicroChemsExperiments]

Hafez Quadri, we test milk sample regularly at our lab following this method and get the accurate results. Even we test yoghurt & different milk drinks samples for Fat determination by this method and this method is recognized internationally. So this method works properly. Try this method again. If you face the same problem then check & maintain these points given below: 1. Gerber H2SO4 should be prepared in the right way as described in this video. 2. Iso amyl alcohol should not be expired 3. Centrifuge with Gerber Centrifuge machine 4. Heat the mixture in tube at 65 degree for 7 or 10 min instead of 5. 5. You can centrifuge more then 5 mins (Example: 10min). 6. Follow all steps strictly as shown in this video.

[@aacreatives3117]

@@MicroChemsExperiments Hi, Can we use this same method for the fat estimation of whey drinks? Does this complies with AOAC method?

[@MicroChemsExperiments]

@@aacreatives3117 This method is mainly designed for Milk and milk product analysis. But you can also analyze Whey drinks also using this method. This method is complies with Gerber Method completely.

[@darshanawajira3826]

thankxx

[@MicroChemsExperiments]

Welcome

[@RecepEVYAPAN]

How to fat determination in butter fat by gerber method

[@MicroChemsExperiments]

Take 5g butter sample and do test as done in this video.

[@RecepEVYAPAN]

@@MicroChemsExperiments Thanks

[@chamathgunathilake5602]

How to measure the fat percentage of the milk ???

[@MicroChemsExperiments]

Already discribed in this video. Please watch the video again

[@DON_AYI]

Can i test fat in yogurt by Gerber method?

[@MicroChemsExperiments]

Yes. You can

[@yousraboukssessa4849]

Hello please i need rose goteilbe méthode .

[@MicroChemsExperiments]

Noted

[@yousraboukssessa4849]

@@MicroChemsExperiments thanks you

[@arunapradeep4443]

Sequence of adding completely wrong. Acid first.

[@soldiersachin9483]

How can I I increase a fat or snf in milk

[@MicroChemsExperiments]

You can increase fat or snf by reducing the water content of milk. Heat treatment can be applicable for this purpose.

[@hhmn3018]

Plz do adulteration tests for milk

[@MicroChemsExperiments]

Okay

[@bappaghosh5316]

Very nice

[@MicroChemsExperiments]

Thanks

[@ommaditya445]

Can you use any other alcohol instead of iso- amyle alcohol ???

[@halimaaktermitu8762]

I have a question.. We see that golden color fat are Rising up but how can i know that It's 3.5 %? I wish u'll give reply...it will help me to answer in the time of viva.

[@MicroChemsExperiments]

There is a marketing as percentage on the butyrometer. You can find the percentage of fat by following the marks on that tube

[@andyjones6386]

Would it be difficult for a real human voice to be the narrator? I see videos like this and just click them off. Such an informative video- but such a lazy delivery.

[@MicroChemsExperiments]

Sorry for that. We took help of narrator for few videos at the early stage. Check our latest videos in which we have been using real human voice.

[@07mohdshahid53]

This is wrong method of determining the fat percentage

[@MicroChemsExperiments]

Okay. Ignore the video

[@ishanpatel2190]

Who is the speaker?😆

[@MicroChemsExperiments]

Different person