Microscopy: Cameras and Digital Image Analysis (Nico Stuurman)

Learn more: www.ibiology.org/talks/digita...
This lecture describes how digital cameras for microscopes work, what a "pixel" is, Nyquist sampling, the dynamic range, noise, and color cameras. The features of an image is described, included bit-depth, intensity scaling and histograms, file formats and basic image processing.

[@bilgeargunhan9361]

I've been performing fluorescence microscopy experiments for years and was mostly trained on the job. Having watched this video, a lot of things that didn't make sense before, both in data acquisition and analysis, have clicked. And it only took ~30 minutes. Must-watch for all students/researchers employing fluorescence microscopy experiments!

[@seamus9898]

Resolution limit = 2* (pixel_size / Magnification) This is literally all I needed but it was nowhere to be found. Thank you for this!

[@jeremyschissler337]

Would love to see these updated being almost a decade old

[@Helmet_Tester]

You are a Great Teacher! I really enjoyed this Lecture and am grateful for all the people who take the time to post these videos. Best Regards, Jerry

[@barboramartinkova8452]

I totally love your videos, Nico. They are easy to follow and very well-explained. You helped me a lot. Thank you very, very much!

[@leif1075]

Why are higher bits necessaroly.brighter..that's not true necessarily..they might all be darker and therefore dark..

[@roloman1987]

Thank you so much for doing this. You were incredibly clear and concise. Very very useful lecture.

[@genefreak2]

Thank you! That was a fundamentally useful tutorial. Respect!

[@Anilkumar-zz6lo]

I am in love with your teachings. Keep going sir. Thanks

[@ornestebuitkute9720]

This is really good, thank you!

[@Ishijah1]

Excellent explanatory skills.

[@jaychiang1688]

Wonderful explanation!

[@tymothylim6550]

Thank you very much from this video! I learnt a lot even though I am not doing biology :)

[@Theodorus5]

Good job Nico

[@jeanpaulpadillafabian1639]

Great explanation about image analysis ! Where can I get more information about the software development? I would like to apply this for analyzing mineral microscopic images. Best regards, Jean.

[@nonameartiiist]

I love your t-shirt btw!

[@rasoolgh5025]

Can I use a normal digital camera and analyze the particle using the image I take with it ? what is a proper lens for it ?

[@greenbeecolony1911]

Thank you good video

[@ratan483]

sir plz exaplain how the three intensity mix to find original image

[@abbasalfaouri9955]

what is photon conversion factor? can you make it in front of us?

[@user-zw3gx9rb5v]

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