Microscopy: Darkfield and Phase Contrast Microscopy (Edward Salmon)

[@LaurentLaborde]

he's obviously in front of a green screen and totally not used to it. The guys in comment that mock him : i'd like to see you try it :D

[@TheDarcWolph]

8:40 says NA for condenser is less than objective, but NA_cond > NA_obj

[@manudehanoi]

the back ground slide at 16:30 doesnt make sense. If the diffracted light is focused on the quarter wave plate, then how can it be focused too on the image plane ? Moreover if the planar/collimated illumination focuses on the image plane, meaning the focale distance is the image plane distance, then how is it that the specimen is focused at the same point ??!! The specimen could not have an image if it's closer to the lens than the focal distance, and if it's further away then the image must be further away than the focal distance....

[@mr702s]

@30:00 that is brightfield not darkfield. Was looking for Darkfield phase contrast content.

[@josephoregan7385]

Excellent explanation. Thank you.

[@mehulsharma5228]

I didn't get it. what exactly was done/happened to the phase of the undiffracted light and how was it different in the earlier method without the circular disc ? Also, what purpose does converting the light rays into annular cones serve ?

[@xianglu8583]

+Mehul Sharma his understanding of the principle of phase contrast microscope is wrong....

[@mehulsharma5228]

Entirely or Partially ? could you help me out with a precise understanding of the principle ? Like explain it or give me a link that is more precise

[@sallysassa]

Many thanks for this excellent information. I have an Olympus BX50 and have never known the complexities of the light delivery in my microscope... until today.

[@Mystichandworks]

Great presentation. Thank you.

[@Pettyorpretty]

is there any way to see lipids stained with a fluorescent dye Nile Red under phase contrast microscope? Plz tell if anyone knows because I don't have fluorescent microscope

[@giaiaspirit]

Thank you!

[@kaleemullah8142]

The presentation of this lecture can be improve.