Microscopy Part 8 - Fluorescence Microscopy Basics | Principle and Working | In Hindi

In this video tutorial we talk about Fluorescence Microscopy Basics principle, applications and working in Hindi
In fluorescence microscopy, we use UV light (or shorter wavelength) to illuminate the sample labeled with a fluorochrome. The fluorescent dyes are the molecules that absorb light at one wavelength and emits lights at a longer wavelength giving a fluorescent effect to the sample.
First, the sample is stained with fluorescent dye. “It is not necessary to stain a sample. Any fluorophore (like GFP) can be visualized with a fluorescent microscope.” The parts of the cells can be stained with different fluorescent dye so as to distinguish between them. FISH (Fluorescent In Situ Hybridisation) employs the use of fluorescently tagged probes which binds to a specific region of the chromosomal DNA.
Microscopy Tutorial Series Links:
Part 1 - Introduction : • Microscopy Part 1 - In...
Part 2 - Resolving Power & Image Magnification : • Microscopy Part 2 - Re...
Part 4 - Lab Demonstration Inverted Microscope
• Microscopy Part 4 - In...
Part 5- Bright Field Microscopy Basics | Principle and Working | In Hindi :
• Microscopy Part 5- Bri...
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Mam can you please make videos on confocal microscopy, Epifloresence, FRAP,FRET

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I just wanna relate a things like this : We are like an Electron that are stable in ground state But U are PHOTON for us that make us exited for study , now We are going to higher energy state , during this process U give us energy of understanding power , learning capacity and finally we do our best in study and shine like Fluorescence....

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Most welcome 😊

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Please make video on confocal microscope because your videos are so helpful and good .

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[@A12966]

nicely explained ! kindly let us know that what is the procedure to select the specific wavelength which we want to fall on our stained sample. and the light which we want to fall on our stained sample is matchable with the stained sample ? how this procedure will be done.

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[@mydoctorhealthyzonedrAnubhav]

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[@memories..1267]

If wavelength is more then resolution will be less then how can we visualise..?? I'm not getting this point Mam... Plz tell

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Good afternoon mam, I am pursuing graduation from B pharma.so these topics have been mentioned in my syllabus (vii sem.)

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[@mydoctorhealthyzonedrAnubhav]

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awesome

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Thank you mam

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[@mydoctorhealthyzonedrAnubhav]

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Thankyou mam❤️

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Is it possibpe to stain inclusion bodies in sh- sy5y cell lines with some dye

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Ma'am please make video on stereo microscopy and polarising microscopy....

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Please make 2 audio tracks 1 english 1 hindi for non hindi international audience! Nice easy understandable explanation in this video !

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I always skip microscopy topic but i think its become my fav topic after ur videos..thank u .❤

[@mydoctorhealthyzonedrAnubhav]

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Nice mam

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Mam please upload the Immunoelectron Microscopy .

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[@baberdoger6150]

Mam ap ny bht acha smjya lekin aik confusion hai k blue color ki light ya koi r light excitation filter sy nikly gi wo flourophore pr depend ho ga ya filter ko kis trah set krty hai

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Madam, kindly tell me about the main difference between fluorescence microscopy and fluorescence spectroscopy? Is both are the same??

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[@anjalisharma314]

Ma'am.. 1.Can adsorption capacity of an adsorbent in a sample can be analysed by Florence spectrophotometer? 2. Is the purpose we want to solve with uv-vis spectrophotometer can be solved with Florence spectrophotometer, if uv-vis spectrophotometer is not available?

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Thank you ma'am.

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Arti would you please make a video on confocal microscopy

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Mam confocal laser scanning microscope pr ek video banaiye

[@aditisahu589]

Madam, in this technique, with which method do you dye it? please reply

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Thanks mam..... Mam please TEM ki video bhi jaldi upload kijiye