Microscopy: Phase, Polarization, and DIC (Stephen Ross)

Learn more: www.ibiology.org/talks/dic-mi...
This lab illustrates the components in the optical light path and how they need to be aligned to achieve proper 1) phase microscopy, 2) polarization microscopy and 3) differential interference microscopy.

[@Dhelira]

Very enlightening. Thank you

[@HaloGrndr]

Any chance you could provide some part numbers for the polarizer/analyzer components? I can't find anything!

[@ClownWhisper]

I just got an older TSM inverted microscope and I was wondering is there a camera mount or a camera made that specifically fits the neck of this microscope? I have seen what I thought was a TSM equipped with a completely different neck with a camera going straight up and the regular binocular assembly in its usual position. I have no idea what I should look for can you please help me?

[@AMadScientist]

Hello. Thanks for explaining. What I'm trying to find out is how a DIC scope would view live protists? I see videos on KZbin with these great clips using a DIC but I was wonder if it is just "drop the water on the slide and go" or is there tons of prep time to get the "good" shots. Thanks

[@source1289]

was the lower prism left in for phase contrast ?

[@Thesignalpath]

Thank you.

[@johndubose6589]

Thankyou!

[@Satchmoeddie]

Where can I rent one of these and all the goodies for 2 hours? Amazing scope! Nikon, Canon, Leica, all make nice stuff. I need to do some color phase shift work on 4 samples. That is a great unit! How many human kidneys does it cost?

[@abskateboard]

not to mention zeiss lol

[@JakeSchonhoft]

Can't forget Olympus. Been making microscopes since 1919.

[@donsimons9810]

color phase shift work? not to dork up the lingo, but what do you mean by that?

[@MrGunzoller]

Why use expensive DIC method while you can just use oblique lighting and to see absolutely the same 3D effect? You don't even need a specific instruments, just ordinary biological light microscope. All you need is to play with condenser diaphragm (about 50% closed), opaque screen in front that diaphragm and light volume.

[@donsimons9810]

do you have a reference for this technique used in microscopy?

[@MrGunzoller]

@@donsimons9810 kzbin.info/www/bejne/pZS3dolsf7SgjMU

[@FieryCoal]

Although Oblique is useful for its cost, it gives off a lower image quality than DIC.

[@abedhossini5793]

boring not sobject yor act