cDNA Libraries and Expression Libraries | MIT 7.01SC Fundamentals of Biology

[@黄越-u4w]

When that student said “Human cell”, i was like, nah, must be bacterial or yeast cell, then out of my expectation, the teacher went “That’s interesting, let’s put it as an option”. That is the moment i saw the light of science, education and my ignorance... Great lecture

[@goncalocandeias3309]

and the difference between a good and bad professor

[@MA2520]

actually Eric Lander is so good that I watch these lectures for entertainment (and learning of course)

[@nohemie4057]

That's what I call teaching! I wish I had more incredible professors like this ! Thanks a lot !

[@martinahejnickova3090]

Oh my god, I wish I had a teacher like this! Perfectly understandable AND funny? It doesnt feel like studying anymore :D

[@godslove7301]

Same. i wish my lecture actually care if student understand the material or not. all the care about if getting to the of their prepared slides that's all

[@Redox23-X]

The way he filled the classroom with positivity and curiosity together is just awesome

[@richdog490]

He does a great job of making it interesting

[@fairytaillover8]

eric lander saved my sorry self today after i zoned out in class thank you based god eric lander

[@soniaorjuela8050]

Amazing! Wish that was my professor!!!

[@daniellebelize1862]

Well done. I almost got teary-eyed. I miss college!

[@prashantkumarparmanu]

he is the ultimate teacher of Biology.

[@samaben4812]

Incredible teacher, MIT students have lot of chance

[@KaranKapuria]

Wonderful Lesson a midnight before my exams

[@calebm9000]

This guy is great. Pretty fast for my brain but his energy is infectious. Jealous of those students!

[@Fatheha95]

I have to write an essay on this, and was unsure of the steps. Thanks for the clarification, i wish my lecturers were this good at explaining!

[@Zk_Vist]

@ 7:14 - 7:32 One question, I suppose we're growing Beta Globin cells for the purpose of using it? If the antibody sticks on the Beta Globin cells, these cells become un-usable? Anyone who knows the answer, pls share your knowledge.

[@jackc7069]

I suppose we'd grow these cells in order to use the beta globin itself (as in the example of insulin at the end). In that case, attaching the fluorescent antibody to the cells would kill them in the process because, as he explained, you'd first have to destroy their membranes and spill out their content. Thus the cells die, but the beta globin is still usable.

[@Zk_Vist]

thanks a lot. now I have some questions. 1) how do you destroy their cell membrane without denaturing the beta Globin protein? 2) and how do you isolate the beta Globin protein from the mess? looking forward to your reply.

[@jackc7069]

Leon, Ye Lin Kaung 1) There are many ways to break down the cell's membrane (a process called "lysis"): repeated freezing and thawing, sonication, homogenization by high pressure, filtration, or permeabilization by organic solvents. The method of choice depends on how fragile the protein is and how sturdy the cells are.

[@jackc7069]

Leon, Ye Lin Kaung 2) This usually depends on the mass of the protein. Anything that weighs more than 10 kDa can simply be extracted and purified by a centrifuge (either zonal or differential). Beta globin is just under 16 kDa, so that should do the thing.

[@jackc7069]

Leon, Ye Lin Kaung Otherwise you have to rely on other techniques that target other characteristics of the protein: electrophoresis (separates in function of charge), chromatography (in function of mass, charge or strength of its bonds) or simply an enzyme that binds to certain proteins

[@kritikar2939]

I agree with u guys.. I like the way he taught that, he made it look so simple. I wish to learn more from him...

[@Elif-eo5dn]

The lecture is a legend.

[@victorleon3925]

The best teacher ever...

[@petermurithi1670]

This is my permanent teacher

[@mohammedkulaibi1351]

what is great professor, i wish to learn from one like this

[@hafizaamna3423]

such a cool professor

[@silviapabon1282]

these Master is awesome... hahah I am working in 4 sec the daily question .great

[@kundhan829]

its in the catalogue. love from india

[@nasserismail2438]

This guy makes sense to me.What a wonderful lessons....Thanks% you are a mazing

[@ThePhotogalleria]

Wonderfully presented!

[@marlenesoifer7219]

I'm doing good with this time thanks to your help

[@wenguo66]

Really a great teacher!

[@yasiraa7919]

He's preparing them to be inventors.

[@Devoidzylon]

Learning in the United States seems so easy. In India, in the same amount of time, you need to process five times more data due to the competition and resulting higher floor of knowledge.

[@macielrodriguez6697]

How so? :O

[@MattPresent]

This was wonderful! Thanks very much. Extremely helpful and engaging.

[@mahamkhan9002]

U r amazing teacher 😃

[@field-yetian6001]

I dont get this. If you wanna clone a protein from human gemone, you simply design a specific pair of primers and amplify. Why bother fluorescent ab selection?

[@ivienprinzefetalino3410]

In my understanding, flourescnt antibody will bind to the beta globin protein in the filter paper and this would tell us which cell or colony actually expressed the beta globin gene.

[@ramya.bm.krishna4257]

Wow excellent lecture😊

[@johnvandenberg8883]

Great teacher!

[@songthanh896]

Really helpful, thanks so much

[@leonardosoto4603]

I wish the opportunity to one day study at MIT

[@TlhousemusiclT]

Amazing Prof.!!!!

[@OutstandinMediocracy]

sounds like we have the same professor :P, except mines with a french accent

[@edok2306]

sun everything comes from it, photosynthesis, next next. and d 4 terrestrial plan will take when suns color is our red.

[@fvveb2141]

perfect lecture

[@150iSolony]

I would like to have such a teacher. Chciałbym mieć takiego nauczyciela.

[@malumaha5821]

Pls upload elaborately about chromosome walking

[@moussaouiahmed]

oui exacte

[@Bob-fj7lr]

bookmark 11-23-23

[@TheLastSoviet22]

Make a Protein! ;)

[@chetan4851]

flawless teaching... but dumb class nobody answers the questions

[@jonjon11882]

Right? This is MIT right? I go to UTEP (not even a tier 1 university) and I knew how to make this contruct right away and I don't even have my bachelors yet.

[@christopherdonaghue2461]

Jonathan vazquez This is 7.01 - an INTRODUCTORY biology course.