Monoclonal Antibody Production

[@elisemccarthy5582]

Fantastic description and images. Really well synchronised and understandable.

[@troyna77]

your video deserves much more exposure. this was very informative. thank you!!!

[@benvogel51]

This was literally exactly what I was looking for. Succinct and informative. Thank you!

[@tenochtitilian]

Wow, I knew the dumbed down explanation - but didn't realise the detailed process was so complicated. Thanks

[@EugeneHuber]

Great video. Could you please do one specifically for humanized MAbs?

[@kCyAng93]

Thanks so much, lifesaver! You summarized my MANY lectures in < 5 minutes (was watching @ 1.5x). :D

[@Ns205]

U DA MAN JOHN U DA MAN

[@Wlucey]

This is such a good explanation! Thank you!

[@inhibited44]

Thanks again for explaining the detail of stuff . It's interesting to learn the material or at least become familiar with it.

[@VictoriaGarcia-ql5kc]

thanks for the explanation!! i can't wait for your next videos! :)

[@yaaami90]

I don't get the step at the end where you rescreen for the specific antigen? You have immunized the mouse with one antigen in the beginning? Or do you obtain multiple B-cells with different antibodies?

[@jerrylumumba7975]

Would you be able to give like what the 4 main steps are in making these antibodies

[@drnyashagogoma5368]

What a fantastic explanation!! :)

[@Queenmania2007]

Great video, clearly explained!

[@TheFreeForever]

This was a fantastic vid!!

[@Wandelaarke]

Perfect video! Thanks!

[@dabo9181]

Can I use CD1 mice instead of Bulb/C for Mab preparation?

[@xinliu1337]

Quick question, the hybridoma should have 4N chromosomes (2N from normal, 2N from tumor ), when these hybridoma mitosis, what will happen? Its progenitors will have 2N chromosomes? If so, if its progenitor split again, will it still have the ancestor all the hybridoma characteristics?

[@meagharamanakumar6608]

thank you john. you made it crystal clear :) :) :) :)

[@gimmichhetry7082]

sir can u plz tell me that the antigen we inject in the mouse is having single epitope or different epitopes

[@PerpetualFlowAudio]

great video! very interesting

[@j2zel]

Anyone know if the myeloma cells are also derived from mice? Since the activated B cells are mouse-derived, I'd imagine that the myeloma cells should also be in order for fusion to occur.

[@ErikThureson]

Well done!

[@wissutachanprasert8577]

This is so cool thanks a lot Really help me here

[@alessiamartina]

He didn't say that the first animal dies, that's one of the reasons why pAb are more widely used, and it explains why only mouse and rabbit mAb exist. For ethical reasons, they wouldn't sacrifice a goat to take its spleen. This is what a professor told me.

[@hudafaisal3860]

you are awesome!

[@mimib1834]

thanks so much you're the best

[@thanthtooaung2979]

thanks too :-)

[@MariamKamel3]

u're a genius!

[@nunzinho91]

Good video ;)

[@greenroofmobilhomes4653]

bác Sỹ John giỏi quá

[@Ns205]

FREAKING HAAAAGOP

[@farhanaanni910]

U should have talk slower so that every single student from different countries also get these... and i think u should have talk lesser and write more so that the advantages and disadvantages which u were describing could be visualized by the reader...i think that would help me more