Hybridoma technology | Monoclonal antibody production using hybridoma technology | Animated biology

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Don't know if the spelling of "first" is wrong deliberately or accidentally....

[@animatedbiologywitharpan]

It was a typo I think

[@yiwang152]

I am a Ph.D of biophysics. This is my 1st time understand how monoclonal antibody is created. Thank you.

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😢😢😢😢

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Hi I am a biomedical and molecular sciences student

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Mean while I am in 11th watching this video 😅😅

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People like you keep the world moving forward by providing hours of free educational content. Keep up the great work! Liked and subscribed.

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This literally saved an hour of studying before my exam. I can peacefully go to sleep now. And wake up fresh for the exam. Thank you so much.

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same. he saved my sleep

[@Visibility-1]

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[@pratishnabhattacharya]

Omg😨this was so complicated in the book .still can't believe I understood this in 8:53 mins only!!!! thanks ☺️☺️

[@animatedbiologywitharpan]

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Congratulations Sir on your appointment as Scientist C at CSIR IGIB

[@abhyarthanabehera4802]

You taught this in 8mins which professors can't do in 45 min class.

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Wowwww..... Excellent animation and video.

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[@arkopalsen8538]

00:02 Hybridoma technology is used to generate monoclonal antibodies. 01:08 Generate monoclonal antibodies using recombinant DNA technology and mouse spleen cells. 02:11 Hybridoma technology is an efficient solution to generate immortal antibody-producing cells 03:21 Hybridoma cells can be selected using HAT media 04:21 There are two pathways for generating nucleotides: denovo pathway and salvage pathway. 05:37 HGprt minus cells would die because they cannot generate nucleotides. 06:51 Monoclonal antibodies are produced in an industrial setting 08:00 Industrial settings and lab settings are different

[@animatedbiologywitharpan]

nice summary and helpful timestamps. Hope that you have liked this video

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I just found out your channel and I really enjoyed and understand the animation. This is the 2nd video I watched in a row to help me study for my test, so now I'm a subscriber. Thank you for sharing💕

[@animatedbiologywitharpan]

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[@dhanumoksha]

This is the best video of monoclonal antibody & Hybridoma technology I came across. Thank you😇

[@animatedbiologywitharpan]

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[@vandanathakur7100]

Really a very good explanation 😍👍I saw many videos but wasn't satisfied with the explanation and I am glad I ended up here. Keep going sir!

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[@EstherIkede]

Same here

[@sadekhossen8745]

Alhamdulillah. It helped me a lot. I was searching a video which would be very helpful but I was not able to Find. Finally I got it. I have never seen this kind of effective and efficient video tutorial regarding this advanced topic. Thanks a lot Arpan Sir. 🇧🇩🇧🇩

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[@selenarivera6945]

This is just crisp and perfect. Saved a big time. THANK YOU SIR!

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[@felicitasrehmann1047]

Thank you so much for this clear and easy explanation of such a complicated topic!

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[@sukanyabose8764]

Thank you sir. I m sure that I will remember this concept till the end as it is so easily understood in an animated fashion.

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These videos are legit life saving 😭😭😭😭

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[@drchandansahoo]

Very concisely and easy way teaching the complex procedure in books.

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[@sturdybirdyy]

This 8 minute video were atleast 60 slides of my presentation. Thanks a lot fr. So easy

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[@KhushiSharma-zb7fd]

tomorrow is my exam. I have watched a number of videos but this is the best one. It made everything so easy

[@animatedbiologywitharpan]

Share this video with your friends ASAP and help them as well

[@vaibhav4622]

I was thinking that hybridoma technology is very hard to understand but you make it very simple and easy to understand. ..... thank you.... 😊for providing helpful vedios.

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

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Tomorrow is my exam and you saved an hour. Bro❤❤❤

[@animatedbiologywitharpan]

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[@lavanyakumari6603]

Very good explanation 👌👌👌... will surely share to my friends...

[@akankshakadukar7046]

I trying to understand this technique from last 3hrs ... Bt i didnt get Bt after seeing this video i cleared all my doubts... Thank u so much sir ji... Lots of love from Maharashtra ❤🥰

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[@akankshakadukar7046]

@@animatedbiologywitharpan ...ok sir n U deserve big audience surely 🥰....

[@thoughtspotential279]

What a beautiful explanation! Thank you Sir! Very Helpful 😊

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[@Alex-ii8zg]

Love the CGI ! What are medicine relies on?!

[@poorvithar1861]

Best video ever❤ thankyou do much

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[@Ushadancercover]

Thank you so much for this video🙏🙏 I was thinking of leaving this topic but you saved me today. I will write thoroughly if they ask a question about this topic. Thank you once again😌🙏

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[@analiliallamas3481]

Super helpful and easy to understand. Thank you!!

[@animatedbiologywitharpan]

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[@pruthvirajbaraiya5077]

I'm stuck with hybridoma tech.but you make it simple 🔥🔥

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[@rachmiputri]

your explanation is easy to understand!! i love it

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[@dribee2019]

Loved one sir😍😍🥰👌

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[@wairimualicekuria4964]

This was an interesting lesson, and easily understood

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[@sereenyasser4969]

thanks for saving my grades many times by your videos🤗

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Life saver!

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[@vaibhavsalunke6940]

Great Explanation Sir...❤👏

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[@nilanjanmukherjee8043]

best video on hybridoma technology thank you sir 💟🤗✨

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[@varshagr606]

The best for 1st year mbbs

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[@sakshisingh1614]

Sir where is your vid for industrial production of monoclonal antibodies?? Pls link it..I cannot find it.

[@tharushiwijetunge6238]

Thank you so much. I think this would be very useful to pass my Immunology paper

[@animatedbiologywitharpan]

kzbin.info/aero/PLKtiwIJ8Q7rq77W7r4cXPrbhi_VpLWw8c&si=ghGvJMWYRRN-OgRC this is the entire immunology playlist. Please checkout before exam and share with friends

[@karenbahenadedios5711]

Excellent explication. Thank you!

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thank you this was very helpful and cool

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Amazing explanation!!!!

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[@MrGregoryMuhs]

Hey dude, I am only a few seconds in, and I love your energy, and I love the illustrations. The sound quality really bothers me though. I recommend a good, but inexpensive microphone. I personally use the Blue Snowball. I also recommend some inexpensive sound barriers on your wall. And finally, there is free software out there for sound editing. I think that with those changes, you will go very very far! Even with just a few seconds, I can see that you are a man with a talent to teach!

[@animatedbiologywitharpan]

Dear Gregory , thanks for the kind words....thanks for your suggestion. Could you please share the Amazon links @ [e-mail]. It would be really helpful. Thanks again. Lots of love from India 🇮🇳

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Nice explanation for a very complicated subject thank you very much it was helpful 👌

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Awesome explanation sir .Thank you so much 🔥

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Grate explanation 👏

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[@smitanayak5898]

best explaination, thanks

[@AbhishekMeena-n8m]

Very nice sir I was very terrified to this topic but I don't believe that I understand it only in 8.53 minutes

[@animatedbiologywitharpan]

Please let us know which topics has terrified you via email ([e-mail]). We will help you with 10min video links. All free all for good.

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Extremely helpful

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Helpful . Like it👍

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[@وسيمصوفان-ك6ه]

Why myeloma cell die when applying HAT media? I don't think the answer is just because they cannot divide. What do you think?

[@manulukusa378]

Really well explained! Thank you very much!!

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Saved me a lot of time ❤❤

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Thanks for the free gift 🎁 of knowledge ❤

[@animatedbiologywitharpan]

thanks a lot

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Thanks for having time

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Excellent teaching sir ☺️

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Wonderful.. thanks

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Thatt was sooo helpful thankyou soo much

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[@sotiriosvasileiadis7260]

nice video overall... but are you sure that polyclonal antibodies are those where the same antibody identifies multiple epitopes?

[@animatedbiologywitharpan]

Yes i am sure. Polyclonal antibodies (PAbs) are a mixture of antibodies that are secreted by different B cell lineages. These antibodies are actually a collection of immunoglobulin molecules that react against a specific antigen, each identifying a different epitope on an antigen.

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Really... Very helpful

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Thank you so much sir last topic remaining which covered by watching you video ☺️☺️

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Awesome explanation sir 🙏🙏🙏😁☺️

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Awesome 😎😎😎

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@@animatedbiologywitharpan allready done

[@viveknarayanan574]

Perfect explanation .. well explained 👌 thank u somuch 🔥

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Please don't forget to checkout others videos

[@welebamuesho6591]

helpful video continue

[@animatedbiologywitharpan]

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[@hasnaaahmed347]

That was so clear way ✨ Thx so much 🌷

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[@sohaibali2166]

excellent explanation thank you ❤❤👌

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[@sohaibali2166]

already I did it 🙌

[@animatedbiologywitharpan]

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[@farhairfan9241]

Thanku so much sir...well explained

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[@farhairfan9241]

Okay

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Thank you for excellent demonstrations

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Crystal clear 💚

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Done already...your Biology channel is gonna rule KZbin soon

[@animatedbiologywitharpan]

Hope to bring high quality content soon. Will hope to enhance the quality in near future.

[@paulina-dk4yl]

Where is the industrial production video?

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Life saver😊

[@lukyamuzisudais-y6f]

Good one

[@animatedbiologywitharpan]

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[@ashwini16k]

Sir can i please know why myeloma cells are HGPRT negative cells.

[@animatedbiologywitharpan]

The cell line has that mutation. Myeloma cells do not secrete any antibody themselves and lack the hypoxanthine-guanine phosphoribosyltransferase (HGPRT) gene, making them sensitive to a medium containing hypoxanthine-aminopterin-thymidine (HAT

[@prabhleenkaur7756]

Beautiful explanation

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[@AmruMagdy]

"إن زوال إسرائيل قد يسبقه زوال أنظمة عربية عاشت تضحك على شعوبها، ودمار مجتمعات عربية فرضت على نفسها الوهم والوهن ". الشيخ محمد الغزالي رحمه

[@dboyes623]

Are Humira's monoclonal antibodies made by mice? An video from UK said that it was not, but said it was non-human but not mice related Do you know the source?

[@animatedbiologywitharpan]

I dont have a proper answer for this

[@aminelaaouina7874]

thank you so much man this video was realy helpful✌🏼

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Your animations are so helpful 😃 Thankyou!

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Good explanation sir .I understood easily osm video sir👌👌👌

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Animated explanation always reachable

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@@animatedbiologywitharpan Sure sir

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Thanks this video helps me a lot..! 😇

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Great job!

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Loved the video

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@@animatedbiologywitharpan Sure thing 👍

[@maneeshamani6066]

Superb sir....

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[@vedangisekra9399]

V nice explanation

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[@denishpatel4096]

Nice vedio... Thank you sir👍

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[@nesadigitalpro]

excellent video

[@animatedbiologywitharpan]

Really glad to know it was useful. Please follow my instagram page and facebook page. Please share my youtube channel link with your friends and help me to reach big audiance I'm on facebook & Instagram as @animatedbiologywitharpan. Install the app to download notes and flash cards. instagram.com/invites/contact/?i=1p41h314q3fv8& You can support the channel by clicking on the super like icon below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for me

[@zainabjummah7383]

Why we take the B cell from the spleen not from the peripheral blood of mouse

[@CrimeVictimsProtection]

Injecting mice dna to people... really?

[@sachinsajeev3479]

Sir how is this animation made? Which app?

[@animatedbiologywitharpan]

Simple PowerPoint. We have a dedicated course on that. Email us at [e-mail] to know more about the course

[@abhinavaggarwal-vk7xs]

its basically somatic cell hybridisation right? But there is AN IMPORTANT THING that cell lines produced from Somatic Cell Hybridisation results in random deletions of chromosome so how are the fused cell capable of forming Monoclonal Antibodies seperated from other cell lines?? i.e., containing chromosome for making Antibody??

[@EstherIkede]

Thank you so much, I now understand it!

[@animatedbiologywitharpan]

Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.

[@muskanray-hx7fy]

I have a doubt. Myeloma cells are immortal so how would they die after fusion ??? Even if they lack HGPRT but they are immortal in nature

[@animatedbiologywitharpan]

the immortality come from the fact that they can devide unimitedly. If the division capacity is blocked then are no more immortal

[@muskanray-hx7fy]

@@animatedbiologywitharpan okay 👍🏻

[@nishatrehman2673]

Amazing

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THANKYOU 🙌🙌🙌🙌🙌🙌

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Are you a NEET aspirant ?

[@olumideoloyi6880]

Awesome!

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Amazing yaar

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[@sarahhello27]

Great explaination, can u please answer my question- why the myeloma which are cancerous cells do not cause cancer after hybridisation. What’s the science behind this

[@animatedbiologywitharpan]

They will be able to cause cancer like growth if implanted inside any organism. In the atificial situation they show infinite growth

[@sarahhello27]

@@animatedbiologywitharpan ohhh and what about after bring hybridized with the antibody producing cells.. When patients are given these cells..... Aren't they harmful... The cancer cells?

[@Alex-ii8zg]

@@sarahhello27 Finally a great question! Critically thinking! We are essentially here to learn how monoclonal antibodies are produced. Good to know, we’re only learning how they are produced in a cell culture, not human lol

[@sarahhello27]

It was a part of my presentation and after some research I got to know that the myeloma cells are genetically altered in two ways so they aren't harmful

[@Alex-ii8zg]

@@sarahhello27 how are the genetically altered? By editing chromosomes? Funny how the whole process of hybridoma technology as well as this presentation, only render CGI images