Deoxyribonucleotides and Deoxythymidylate Synthesis

Moof's Medical Biochemistry Video Course: moof-university...

[@zdoriksandorik]

Your videos are a real life saver, i have my biochem exam in 2 weeks and i was seriously going crazy when i opened nucleic acids and saw all those pathways, but you not only make it very sorted out and very short and on point, you ask all those questions and cross-reference me to another topics, that really helps a lot. Plus, my professor is veeery thorough, so your material is profound enough, you do not leave out the important stuff and i love how you cover clinical aspects Thank you very much for your work

[@poetlamoureaux2983]

thank you! so much for this video. Not only have you broken it down in such a great delivery, you've helped me so much. ~ grateful citizen

[@ModernMen]

Thymine does actually exist in RNA, specifically tRNA (in the TΨC loop).

[@alliek0]

Thank you so so much for making these vids, watching you fill in the blanks is something that helps me remember the names of compounds and enzymes. Better than Khan Academy imo !

[@MoofUniversity]

Allison Kuo Sweet! Thanks for the HUGE compliment! :]

[@johnlocke4428]

I think you forgot to talk about thioredoxin. Its thioredoxin that goes from its reduced form to its oxidized form. In the process it activates RNR. NADPH via thioredoxin reductase converts the oxidized form of thioredoxin back to its reduced form so it can activate RNR again.

[@annaiyenimelo2640]

Thank you!

[@asmanaik7879]

Thanks a lot

[@YoLLCT]

Why is the methyl group of dTMP provided by N5-N10 methylene THF but not SAM? In the previous video, you said that methyl groups are mostly provided by SAM. Thanks!

[@idawael2630]

I thought it was called nucleotide when it has P attached and nucleoside without P???

[@MoofUniversity]

Ida Wael That's correct!

[@hoaralatoan845]

Thanks you so much for helpful video. But I have a question: Enzyme RNR can reduce ribonu monophosphate like ribonu diphosphate, right? Is triphosphate reduced in the same way? Thanks you

[@yalegordon574]

How does methylation syndrome play into this process? How might it manifest itself behaviorally?

[@bloui1033]

BCH 110B FINAL ON MONDAY!!! WOOT.

[@MoofUniversity]

bloui1033 Lol. Good luck!

[@naasey5506]

Which video do you speak on the cancer drug treatment with the UMP to TMP pathway?

[@MoofUniversity]

I might be a little late with this, but: kzbin.info/www/bejne/oZO8hXSviraal5I

[@kachingchan9228]

May i ask why ribonucleoside diphosphate is named but not ribonucleotide diphosphate? Since ribonucleoside diphosphate contains phosphate groups, so shouldn't it be ribonucleotide? Many thanks!!

[@MoofUniversity]

Ka Ching Chan Sorry about the delay in my response, but the reason is simply because the phosphates are ATTACHED to a nucleoside to MAKE a nucleotide. It's not actually a nucleotide until the phosphates are attached. Therefore, if "(mono/di/tri)phosphate" is in the name, it's a nucleotide (because it has phosphates).

[@danieloren4849]

hey, one question.. why does it matter that i have 2 phosphates on the molecule? would the process look differently with one or three? (AMP,ATP)

[@MoofUniversity]

That's a good question. Although I'm not 100% positive about the answer to that question, I think it's a simple matter of enzyme specificity. Enzymes are structured in such a way that they have active sites that act on very very specific substrates. It's the whole structure-function relationship. As far as I know, this enzyme ONLY acts on ribonucleoside diphosphates. Perhaps it is because ribnuceloside diphosphates fit well in the active site. I'm not exactly sure if this type of reaction is even possible with ribnuceloside monophosphates or ribnuceloside triphosphates. As far as WHY that is, I have no idea, dude.

[@danieloren4849]

thanks man! and thank you for the videos,extremely helpful!

[@MoofUniversity]

daniel oren Sure thing, yo.