One Carbon Metabolism | Tetrahydrofolate and the Folate Cycle

Lesson on One Carbon Metabolism, the role of tetrahydrofolate and the folate cycle. Tetrahydrofolate (THF) is derivative of folic acid (Vitamin B9), which is acquired from dietary sources. Tetrahydrofolate (THF) in various forms (N5,N10-methylene THF etc.) acts as a carbon donor, allowing cells to produce nucleic acids, amino acids and methionine and s-adenosyl methionine in the activated methyl cycle.
**Re-uploaded to correct mistake**
Hey guys! In this lesson you will learn about one carbon metabolism, tetrahydrofolate and its role in producing various cellular products needed for purine and pyrimidine synthesis. You will also learn which dietary sources contain folic acid (folate), how folate is processed to tetrahydrofolate, and you will also learn the enzymes of the pathway. The anti-cancer medications known as methotrexate and 5-fluorouracil and their enzyme targets will also be discussed. Finally, you will also learn how the one carbon metabolism pathway is connected with the activated methyl cycle and methionine metabolism.
Hope you all find it helpful.
JJ
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**MEDICAL DISCLAIMER**: JJ Medicine does not provide medical advice, and the information available on this channel does not offer a diagnosis or advice regarding treatment. Information presented in these lessons is for educational purposes ONLY, and information presented here is not to be used as an alternative to a healthcare professional’s diagnosis and treatment of any person/animal.
Only a physician or other licensed healthcare professional are able to determine the requirement for medical assistance to be given to a patient. Please seek the advice of your physician or other licensed healthcare provider if you have any questions regarding a medical condition.
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Although I try my best to present accurate information, there may be mistakes in this video. If you do see any mistakes with information in this lesson, please comment and let me know.
I am always looking for ways to improve my lessons! Please don't hesitate to leave me feedback and comments - all of your feedback is greatly appreciated! :) And please don't hesitate to send me any messages if you need any help - I will try my best to be here to help you guys :)
Thanks for watching! If you found this video helpful, please like and subscribe!
JJ

[@andrewjames2475]

You did a fantastic job laying this out I was getting very confused as I review for my upcoming exam and this is much more comprehensible than my notes. Thank you very much!

[@samuelsowah]

Thank you so much Its been 6 years but its still great. Now I need to find a way to remember this 😅😂❤

[@filippodare5094]

amazing video, but I have to say that you have made a mistake: in the reaction catalized by MTHFD you said that NADPH is oxidized, but at the same time you are oxidizing methylene THF to ethenyl THF; so it should be that NADP+ is reduced to NADPH.

[@abood0709]

i love this so much. still dont understand but i love this

[@jumanh745]

best explaination I’ve ever seen

[@team6021]

it was really good but u can also tell about folate trap

[@kathy2659]

Thanks, I missed the lecture on this. Helped me understand the lecture slides :)

[@redietyonas3079]

Thank you very much sir . This video was super helpful .

[@PieroMorandini]

I beleve there is a mistake in the scheme concerning the reaction catalysed by MTHFD. Since the THF-methylene becomes a THF-methenyl, the substrate is oxidised (abstracting two electrons and 2 protons) and therefore you require NADP+ as a co-substrate and produce NADPH as a co-product, not the opposite as drawn in the scheme.

[@d7mii_amoudi957]

Legend❤️

[@dhariq8772]

THF are needed by many enzymes for the synthesis of A.A and its a coenzyme in vit B9

[@baraskparas9559]

Superb presentation of a difficult topic. Thanks

[@strugglingdoctor7437]

Supervv video sir

[@fttcfgv4550]

thank you thank you!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!

[@giacomomari4916]

thank you very much, u save my life cheers from italy :)

[@gabbyjams7223]

Why does SAM have a dashed line back to homocysteine? Would it not send homocysteine to Succinyl coA pathway?

[@bacanimichael]

This is an amazing video and explanation ! Thank you

[@delminmaria4040]

Well explained...

[@farwacontacts8601]

Very helpful

[@manar6501]

just put ads at the beginning of the video or the end it's very disturbing, and thank you so much for the effort

[@yasirmohammedali]

Hi jj ... Thank you

[@045jerson7]

you are simply awesome !!!!!!!

[@senadonkor2228]

Thank you so much for this !

[@hema11901]

nice

[@christineson6932]

Amazing ❤

[@samudrabasu150]

God level stuff

[@stephenleighton4050]

Question..... can anyone explain or give clarity on the following ... does any change to typical Purine & Pyrimidine, folate, B6 or B12 pathways during pre or early post natal periods result in heritable genetics that could be passed from that baby should they procreate in later life?

[@shahzaibmajeed1193]

Surat No. 2 Ayat NO. 155 اور ہم کسی نہ کسی طرح تمہاری آزمائش ضرور کریں گے ، دشمن کے ڈر سے ، بھوک پیاس سے ، مال و جان اور پھلوں کی کمی سے اور ان صبر کرنے والوں کو خوشخبری دے دیجئے ۔ Surat No. 2 Ayat NO. 155 And We will surely test you with something of fear and hunger and a loss of wealth and lives and fruits, but give good tidings to the patient, Surat No. 33 Ayat NO. 11 یہیں مومن آزمائے گئے اور پوری طرح وہ جھنجھوڑ دیئے گئے ۔ Surat No. 33 Ayat NO. 11 There the believers were tested and shaken with a severe shaking.

[@sameerahmedmughal1534]

What's the point of mentioning such stuff here ?

[@nebukednazar]

perfect

[@keatonbuddy656]

How does formaldehyde play into this cycle??

[@bluejay8836]

Bacteria are also a huge course of folate for us

[@lucianomenesess]

you are a f*cking hero broooooooooo

[@r_aorwa]

👌♥️

[@inayatullah1321]

VITAMIN B COMPLEX BIOCHEMICAL PATHWAY?Any one who can explain

[@pietrocantuccini5584]

Get yourself a copy of "Gerald F. Combs Jr., James P. McClung - The Vitamins (Fundamental Aspects in Nutrition and Health) 2017" and you'll be sorted.

[@toto_pep]

:D

[@michellehopelamb5353]

I don’t understand any of this. Why can’t they make this for a lay person who isn’t sick and having comprehension issues

[@daisysolano2477]

Is it 😂a problem to have high folate

[@cartoonnetworkchannel9074]

Yup

[@udaman100]

Not at all if you're insulin sensitive and not activating mTOR all the time.

[@summerbreezenight]

It's so nice that this video has no background music.

[@jjmedicine]

Glad you liked it :)

[@hassanbarada2993]

very clear good job

[@doctorofmedicine1113]

Exclusive Osm lecture .. I have no words to appreciate it.... thanks a lot I'm ur new subscriber .. best of luck

[@music1491]

These lessons are fantastic for med's school, nutrition, and pharmacy students!!!! Thanks for everything that you have done. You teach better than my professors.

[@BarryAnderson]

I consume lots of my organically leafy greens on rotation mixed with cultured fermented foods and culinary forms of WILD GAME meats and wild seafood low on the marine food chain. Holistic Chef Barry Anderson of Phuket Thailand.

[@julietjaime]

Amazing explanation. Tons of greetings from Germany and thank you very much for taking the time to make such good content.

[@kaylahart3820]

I owe you my degree

[@hraqhraq]

I look at it as if Serine is the start point and it charges the Vitamin B9 with the least reduced form N5, N10 Methylene THF which will be charged further by 2nd reduction to become methyl THF, the most reduced the most charged one which now can charge Vit B12 to become methylCobalamin which will then loose the methyl charge to homocysteine to form the final product Methionine

[@tomasheger756]

Hello, Can I just ask - where did you get the information about FAD participating in these reactions from? Do you think there is a reason why FAD should take part in these reactions? Very nice summary, good to see the pathway as a whole.

[@gupiegup]

beautiful. keep up the good work

[@GauravSharma-lx9rb]

great lecture

[@faialnashmi4710]

Thank you so much! Thank you for presenting this in steps and not giving the whole pathway at the beginning, it’s less scary like this😂

[@jjmedicine]

You're so welcome!

[@ErickGon]

Good day! May I know when's the part that of the metabolism is transferred from duodenum to liver? Thank youuu

[@sss-yq6no]

너무달다 ❤

[@mostafaalatweh4850]

Thank you so much sir. That is why when we give the active form of riboflavin we shift the pathway more to DNA synthesis and reduce the amount of methionine synthesis and the catecholamines synthesis such noradrenaline . And this reduction in noradrenaline considered beneficial for migraine patients. Please correct me brother if it is wrong.

[@Colors.TF1]

I take 100ug Methylcobalamin, and 500ug 5-methyltetrohydrofolate. Higher dose of b12 is hard to find in my Country.

[@fabikainz9255]

great video!! explained perfectly

[@sammyberrysunday5989]

Excellent analysis 💯

[@kimjosemanders9684]

Thanks for the explanation! I have one question, where does this al take place? Is this after the uptake from the intestinal lumen into the enterocyte cells?

[@gmed6060]

Hi, thank you so much, but I don't understand how B12 deficiecy stop the dTMP wich important for DNA's synthèse? because THF can become N5 N10 Methylene-THF, and from that we can have dTMP (Thymidin). so I see that B12 deficiency can't stop totaly the Thymidine !!.. Please if Mr JJ haven't time, everyone know the answer can replay me. thanks a lot.

[@reddipallisharath7273]

It is called folate trap. In the absence of vitB12 more folate in the body stays in the form of N5 methyl THF. Thymidylate synthase requires N5N10 methyleneTHF, not N5methylTHF. And N5methylTHF can not be converted to N5N10methyleneTHF

[@mihaigherman3713]

awesome to the extreme; thanks a lot!

[@mariociencia12]

The animation is very good. However, there is a small error in the animation. The synthesis of methenyl-THF from methylene-THF form NADH + H+, that is, this reaction uses NAD+

[@jjmedicine]

Hey Mario! Thanks so much for your comment and pointing out this error - I'll look into it and list it as an error. Thanks again - Hope you are enjoying the videos :)