Multiple Sequence Alignment

An exercise on how to produce multiple sequence alignments for a group of related proteins. Produced by Bob Lessick in the Center for Biotechnology Education at Johns Hopkins University. http:--advanced.jhu.edu-academics-centers-center-for-biotechnology-education-

[@dr3amy3gg]

This was extremely helpful. Had no idea how to input the sequence data. Even though I'm working with nucleotide sequences, it was extremely helpful!

[@AJTalksNerd]

That tip about multi-fasta is going to be so useful. Had no idea you could do that.

[@DevendraDevendra-df4nd]

Alex W xxxve xxxve

[@unknownzodiac9134]

Thank you so much, from about 7mins you told what the dots and * mean and that was enough for me to understand my Homework. THANK YOU!!!!!!

[@LoloBahri]

you absolutely deserve a very very big Thump-Up, Thanks a million

[@sundriedpea]

Hey! Thanks so much for this video! I found it EXTREMELY helpful. If you can, can you please do a tutorial on how you would create a phylogenetic tree using the sequences. Also, what programs will you recommend for this?

[@DividedStates]

Is there a way to automate this for - let's say - 300 sequences? I would need these MSA for evolutionary conservation scoring of amino acid residues (e.g. Rate4Site) [Any better idea?]. I have some motifs, that appear very diverse. For validation, we would like to filter for some measure of biological significance. Anybody? Any idea?

[@angelortegab5]

Thank you very much for posting this video! It helped a lot!

[@shepherdtheo2949]

i realize I'm kinda randomly asking but does anyone know a good place to stream newly released series online?

[@sethbriar8221]

@Shepherd Theo i watch on FlixZone. Just search on google for it =)

[@user-eh7oo8zo3k]

It is extremely helpful! Most of all, servers you used were up to date!

[@jackiezuker6695]

Very clear and informative! Thank you so much!

[@teresagarcia-ybarra6143]

Where did you find the accession numbers for Danio rerio and Drosophila melanogaster? I cannot find them in the organism report. Help me please

[@malikid1000]

I am trying to compare protein sequences from ebola taken from each timepoint during the ebola outbreak, so far I have collected sequences from spike glycoproteins.What tool can i use to compare them, I have tried aligning but there are too many sequences to handle.What can I use to give me a clear picture of any mutational changes or if the virus has evolved.

[@ed3kzz]

Does anyone know a way that you can replace the acession number with the name of the organism the protein comes from?

[@martaperez285]

Thank you for this video!

[@yasmeenfazikazi4092]

How can we do multiple sequence alignment of whole genome of a bacterium

[@chizhang7846]

Thanks for the tutorial. I'm looking for a way to shade the consensus-matching AA letters in the black, positive ones in gray and different ones have to shade. Have you happened to come cross any tool to provide such style? Thanks.

[@rajusrkr5444]

i m computer science student. i want to do research in bioinformatics with data mining concepts. could u please suggest the topic ?

[@DariyusKabraji]

You are a LIFESAVER!

[@bachirgoudia6070]

Thank you so much for this video

[@irinavalsova3268]

How did you copy the accession numbers into the notepad?

[@shreeprasadjoshi]

Ctrl+C Ctrl+V

[@mekhalaspencer1370]

THANK YOU !!! this is very informative and useful

[@AzminaAlisa]

Thank you so much!

[@madhu-ki-duniyaofficial]

Sir,I liked this lecture.could u tell me how do we get STOCKHOLM file format like "PF05371_seed.sth" in biopython. iam getting txt file of it. I want sth file to learn programming in Biopython.

[@MySandaru]

thank you so much for this video​

[@prof.dr.fouadrazzaq6081]

Thank you so much

[@zapy422]

Thank you

[@RohanPatilsm]

really very helpful! Thanks :)

[@Hoatknguyen]

Thanks!

[@MuhammadAsad-cf5vi]

How can i contact you sir?

[@hariszahid5695]

Thankyou!

[@km2052]

tx

[@gauravbisu7102]

Anyone has done MSA in hadoop here !

[@obd801]

Thank you