The Spectrophotometer: A demo and practice experiment

  Рет қаралды 745,056

BioNetwork

BioNetwork

Күн бұрын

The spectrophotometer is an instrument used to measure the effect of a sample on a beam of light. We can learn a lot about a substance by studying the way it interacts with light.
In this video, we'll demonstrate how to use a spectrophotometer to perform a basic experiment: determining the absorbance spectrum of red food coloring.
Included in the video is a short animation that illustrates the inner workings of a spectrophotometer.
Special thanks to Joe Issa and the Gaston College Biotechnology department for helping to make this video.
Practice using a virtual spec www.ncbionetwo...

Пікірлер: 148
@wheresjohn1944
@wheresjohn1944 8 жыл бұрын
very relaxing review before a lab practical
@fariashinshin1041
@fariashinshin1041 2 жыл бұрын
Exactly
@thanushk
@thanushk Жыл бұрын
Exactly
@walsh0
@walsh0 Жыл бұрын
Exactly
@randomdude4360
@randomdude4360 11 ай бұрын
Exactly
@HiddenTalent_07
@HiddenTalent_07 9 ай бұрын
Precisely
@BrennanDemarest
@BrennanDemarest 9 жыл бұрын
Excellent video. Clear cut and well filmed.
@telracsazyl1243
@telracsazyl1243 3 жыл бұрын
no cap. this channel goated
@athiramalukilikood6075
@athiramalukilikood6075 5 жыл бұрын
Thankyou so much, today is my practical examination, and it was really really useful one😍
@vahidmirkhani
@vahidmirkhani 5 жыл бұрын
6:07 What does this plot mean? What's our interpretation? As you can see, the absorbance is relatively high for wavelengths below 600, but 600 above is not absorbed and hence, transmitted. So the photons reaching your eyes from going through this solution, mostly have wavelengths between 600 and above and hence, the red color. I hope this helped a bit.
@Teekay_ZA
@Teekay_ZA 5 жыл бұрын
It helped a lot! Thank you
@noork.631
@noork.631 2 жыл бұрын
Thank u soo much
@leventnergiz
@leventnergiz 2 жыл бұрын
Thank you for your remarkable conclusion. it meant a lot to me.
@davidmonroy3965
@davidmonroy3965 7 жыл бұрын
How do you determine the wavelength that should be used? can it be a constant, and keep it the same for all my samples?
@darksector1389
@darksector1389 3 жыл бұрын
I'm assuming they are using a hydrogen source light where it has a constant wavelength when it enters the spectrometer. Then use this equation: d sin(theta) = m (lambda), where d = grating distance (which is written on device), m = 2 (using Balmer series for visible wavelengths). Then you get four discrete wavelengths of: 410 nm, 434 nm, 485 nm, 656 nm which are the spectrum of hydrogen emission.
@amailyakter6589
@amailyakter6589 3 жыл бұрын
Thank you so much for such an effective demo, I got the idea of how to use this machine
@feminico2613
@feminico2613 4 ай бұрын
bsmls 1b was here
@achlys7290
@achlys7290 4 ай бұрын
wala tayong ganto sa school scamm
@jamalfareedi996
@jamalfareedi996 4 жыл бұрын
One of the best review of Spectrophotometer in lab
@theoryofeverything4237
@theoryofeverything4237 3 жыл бұрын
very nicely explained and the content is so smooth and crisp :)
@토토로솔
@토토로솔 3 жыл бұрын
what is the purpose of using blank solution?
@A_NILAVIDILAUTAMI
@A_NILAVIDILAUTAMI 2 жыл бұрын
Hi, this is a very interesting video and easy to understand. I'm currently a second year pharmacy student, and I am learning about Spectrophotometer. Can I use this video for my college project? I'll give BioNetwork some credit or acknowledgement in my video. Thank you so much, stay safe and healthy!
@NCBioNetwork
@NCBioNetwork 2 жыл бұрын
Yes, you are welcome to use the video as part of your project. Please provide the appropriate reference / citation to this video. Thank you!
@vinhluong3336
@vinhluong3336 2 жыл бұрын
Why does transmittance of a blank have to be adjusted to 100% before recording the transmittance (or absorbance) of sample at any wavelength?
@ChadCarney-hu3du
@ChadCarney-hu3du 7 жыл бұрын
Now I have a way to measure if an increase/decrease of algae occurred in a sample of gymnodinium!
@tinaishemachida9798
@tinaishemachida9798 Жыл бұрын
could have never found a better explanation than this yohhhh!
@The_Bees_Knees93
@The_Bees_Knees93 12 жыл бұрын
have a bio lab practical tomorrow, easily learned how to use it more by watching this video. thanks!!!! :)
@Cosmicvisionz
@Cosmicvisionz 2 жыл бұрын
How can we calibrate spectrophotometer for lower concentrations of iron? Like in 1-5 PPb.
@kusalseven3563
@kusalseven3563 2 жыл бұрын
Imagine working in a lab full of these retro style equipments 😌
@binbinky78
@binbinky78 12 жыл бұрын
best explanation out there on how to use it. Thank you!
@NeuralManifolds
@NeuralManifolds 7 жыл бұрын
At 2:07 what adjustment does the spectrophotometer makes inside when Transmittance is set to 100% while blanking ? what is behind that 100% transmittance knob?
@dr.hebasabry1521
@dr.hebasabry1521 4 жыл бұрын
Same question I need an explanation please
@Naveenbabuborugadda
@Naveenbabuborugadda 10 ай бұрын
What is the blank solution used here?
@infomatics315
@infomatics315 7 ай бұрын
Can this be used for analysis of phosphorus content in plants
@oldboy4782
@oldboy4782 6 жыл бұрын
Relaxing demonstration with fine video shot
@TheViewer318
@TheViewer318 10 жыл бұрын
Hye.. I have a question. If this is for adrenaline test in saliva, how could it help..? the output are all numbers on transmissions and absorbency. the aim to test the adrenaline is to detect stress, and through this test, how could it help? Thank u in advance.
@cophernicus510
@cophernicus510 2 жыл бұрын
That intro music was fire 🔥
@laraibfatima1643
@laraibfatima1643 Жыл бұрын
Sir I have a question ⁉️ 1.If you tell the transmitted light in %....then absorbed light must be in %......but you taught absorbed in (nm) and transmitted in % 2..if 10% is transmitted then 90% should be absorbed or used anywhere else?
@OluwatoyinAdelusi
@OluwatoyinAdelusi 12 жыл бұрын
You just saved me from failing a bio lab practical! Thank you so much!! :D
@telracsazyl1243
@telracsazyl1243 3 жыл бұрын
@@bulletprooftrading its 8 bro
@ibby_h5574
@ibby_h5574 3 жыл бұрын
@@telracsazyl1243 was 6 at the time he commented
@anisarashid1135
@anisarashid1135 5 жыл бұрын
why it's adjusted to 100% T every time wavelength is changed?
@aurynhs196
@aurynhs196 5 жыл бұрын
can you find starch concentration using uv spectrophotometer?
@Dai_rui
@Dai_rui 4 жыл бұрын
Hey are we increasing the wave length?
@rrbuff1960
@rrbuff1960 12 жыл бұрын
I am not totally sure of your question here. The sample concentration is irrelevant for this activity. If you plot wavelength on x axis (380, 405, 430 nm....... ) versus the measured absorbance on the y axis you will find the wavelength of maximum absorbance, called lambda max. Each color has its own lambda max. That is the goal for this particular lab.
@thainguyen5553
@thainguyen5553 11 жыл бұрын
That's awesome! It would help me a lot for my experiment next week :D
@serena3
@serena3 3 жыл бұрын
What will happen if you forget to wipe the cuvette before putting it in the spectrophotometer?
@tamileditingstudios9325
@tamileditingstudios9325 2 жыл бұрын
It will show wrong value.
@gianpd1834
@gianpd1834 8 жыл бұрын
someone know why they didn't make transmittance vs wavelengh tab?
@woosungchoo3282
@woosungchoo3282 2 жыл бұрын
lol why do you increse wavelenth by 25 each time just partice?
@jiteshyadav3466
@jiteshyadav3466 4 жыл бұрын
Sir I want to check copper purity 99.99%. How to check this process.
@josephbenobasa
@josephbenobasa 10 жыл бұрын
This would come in handy for my lab tomorrow
@rajput9s764
@rajput9s764 6 жыл бұрын
Awesome and very useful😊😊
@streaminggames9186
@streaminggames9186 9 жыл бұрын
nice video helped me to learn more
@celticbard9313
@celticbard9313 8 жыл бұрын
Awesome job explaining this, thanks.
@Mauberetl
@Mauberetl 12 жыл бұрын
Can you tell me, what is the concentration of your samples, which you inserted into the sample compartment? I'm confused, varying wavelengths when you use?
@jennifermiranda6998
@jennifermiranda6998 Жыл бұрын
I was thinking the same thing. If the dilutions are made of the same solution, then why vary the wavelength? That and/or molar absorptivity should be the same, unless again, the samples are different. Also, no need to place the blank every time you run a sample made of the same solution (but only being diluted). Blank should be read again if you are running a different solution sample. Again, that's just me. I think they did these customizations for demonstration purposes.
@nayanpanchal5538
@nayanpanchal5538 4 жыл бұрын
How we evaluate concentration
@shyamkumar-cz5ow
@shyamkumar-cz5ow 11 жыл бұрын
thats awesome! but i wanna know why are we changing wavelength of light for same samples?
@crimsonwood5264
@crimsonwood5264 4 жыл бұрын
To get a different absorbance values and use it to plot your graph because in each wavelength length the value of absorbance will change.
@eliyaskebede-v3v
@eliyaskebede-v3v 2 ай бұрын
Very good story ❤❤🌹
@RedGoddesscom
@RedGoddesscom 12 жыл бұрын
Thank you for upload. The explanation how it works is very good!
@Emotions_of_chemicals
@Emotions_of_chemicals 8 жыл бұрын
nice explanation...thanks bio network..
@charleskingsley4290
@charleskingsley4290 11 жыл бұрын
This video was very helpful to me. Thank you so much for the video
@louisegabrielbotastas9583
@louisegabrielbotastas9583 Жыл бұрын
Bery helpul in our chemistry class
@temesgendeka4094
@temesgendeka4094 4 ай бұрын
Can you apload another video for polyvinyl alcohol
@debjitmukherjee8494
@debjitmukherjee8494 11 жыл бұрын
the video was very very helpful to me.,i request maintenance guide about the spectrometer video present it.
@talibmuzafar8836
@talibmuzafar8836 7 жыл бұрын
how to calibrate the spectrophotometer?
@HiddenTalent_07
@HiddenTalent_07 9 ай бұрын
Idk why I find this video so peaceful ahaha
@kevingp12
@kevingp12 11 жыл бұрын
I have a practical too on monday!!!
@atishkumar708
@atishkumar708 3 жыл бұрын
purpose of blank?
@kiribundi
@kiribundi 11 жыл бұрын
Yes, if your concentration is higher, your absorbance will consequently be higher
@raqiamalik9419
@raqiamalik9419 2 жыл бұрын
hi, why you test the sample again can you reply me
@RanaAyan-j6h
@RanaAyan-j6h 4 ай бұрын
To measure absorbance and transmission at different wavelength
@aashinabhatia1612
@aashinabhatia1612 4 жыл бұрын
What's the purpose of blank here
@munkhundarga9081
@munkhundarga9081 11 ай бұрын
VEry nice bro
@jayeelli3906
@jayeelli3906 3 жыл бұрын
how to analyze an unknown substance using spectrophotometry?
@ishanilahiri7387
@ishanilahiri7387 3 жыл бұрын
If you know what kind your unknown substance is like is it a protein or a reducing sugar, then prepare a standard curve of the substance (of known concentration) closest to your unknown substance, and then map it out.
@jayeelli3906
@jayeelli3906 3 жыл бұрын
@@ishanilahiri7387 hi thank you. i love u so much
@chiomaobunadike3313
@chiomaobunadike3313 2 жыл бұрын
I still don’t understand what we would do with these values
@bennettegaper4344
@bennettegaper4344 6 ай бұрын
Thank you so much
@jordanmellis314
@jordanmellis314 6 жыл бұрын
Why did we have to set the wavelength to specifically 380 nm?
@johnfernandes9004
@johnfernandes9004 10 жыл бұрын
THANK YOU SO MUCH
@arbazkohati747
@arbazkohati747 7 жыл бұрын
tkanks for demo and practice exp
@snwbubble
@snwbubble 4 жыл бұрын
why so serious?
@peterluxus7382
@peterluxus7382 5 жыл бұрын
you don't this manually in 2019???
@nicolelevy219
@nicolelevy219 5 жыл бұрын
Excellent
@Sluo1947
@Sluo1947 12 жыл бұрын
that was beautiful :')
@Mobashar61
@Mobashar61 9 ай бұрын
Nice❤
@vincaalkaloids6732
@vincaalkaloids6732 8 жыл бұрын
Thank you very much.
@hezakadoo6514
@hezakadoo6514 2 жыл бұрын
The soundtrack tho 👌
@shahdragb9062
@shahdragb9062 2 жыл бұрын
well explained
@mapalojohnchanda4107
@mapalojohnchanda4107 Жыл бұрын
Absorbance spectrum for red food colouring
@FeliciaAdedotun
@FeliciaAdedotun 11 жыл бұрын
Very helpful....thanks
@zodd0001
@zodd0001 6 жыл бұрын
It takes a lot of time make just one complete spectrum. It must be an old version of spectrophotometer or just a didactic one.
@fss1704
@fss1704 6 жыл бұрын
zodd0001 fking yeah, i build an automatic spectrophotometer in my bedroom pretty much the size of a cigarette box that can connect to my cellphone via non standard bitbanged infrared signals using an adaptor in the audio jack to send commands to the spec microcontroller and receive the digital data via the jack microphone input, i spend 50usd in it and the result is pretty much high grade lab quality.
@fss1704
@fss1704 6 жыл бұрын
Theoretically you can do the read using an USB sound adaptor and bypass the input cap on the microphone, then use the sound output of a moving loudspeaker with a prism glued on top to filter the light, and a photodiode in the mic input to read the level of absorbance on each wavelenght.
@dailygrow6961
@dailygrow6961 3 жыл бұрын
Fascinating to see. Build one from paper on my channel 😂
@deepakchoudhary9146
@deepakchoudhary9146 3 жыл бұрын
Watching in online practical )
@Zept41
@Zept41 6 жыл бұрын
What song is this ? Dammmn
@abdullahdar2527
@abdullahdar2527 Жыл бұрын
Even the man looks like Animated AI robot
@allroundergamer1738
@allroundergamer1738 5 жыл бұрын
Thank you 🎈
@bhartiandana8314
@bhartiandana8314 3 жыл бұрын
It helps alot 👍
@ulfataabid2809
@ulfataabid2809 3 жыл бұрын
I have a qstion sir .. can u please help me sir ..... Make a report about Spectrophotomter from zero to 100... I don't know sir what I write in this assignment .... Becz of covid I never seen this practical .... How can I write .. can u plz help me sir so I can learn something about this and I can submit my assignment... Waiting for ur rply sir
@sanofarnisha5899
@sanofarnisha5899 6 жыл бұрын
Thanks a lot
@Mrsemanmahmoudm
@Mrsemanmahmoudm 12 жыл бұрын
so good
@yxz458
@yxz458 8 жыл бұрын
u don't have to set the transmittance to zero before putting in the blank, since the trans/abs will be changed to either 0 or 100 after putting in the blank solution. this extra step is confusing and unnecessary...
@fss1704
@fss1704 6 жыл бұрын
Joanna Zhang i made a diy version for 50usd and it can plot the results in the screen of my cellphone, and it's a LOT more precise than this "remove x and exchange it by z" error monstruosity. Good fking luck correlating all of this data points from a moving sample.
@doooooms1586
@doooooms1586 23 күн бұрын
❤❤❤
@pinkyy3
@pinkyy3 11 жыл бұрын
Me too!! Ahhhh :S good luck!!
@anannaone
@anannaone 5 ай бұрын
@Digital_Guidance
@Digital_Guidance Жыл бұрын
Show calculation part
@ezg_
@ezg_ 9 ай бұрын
عاش مال تقنيه 😂😂
@indrarayendra5530
@indrarayendra5530 3 жыл бұрын
Dewissengi aga kasi bettuanna
@t.s.m7116
@t.s.m7116 4 жыл бұрын
استمر حبي
@Mauberetl
@Mauberetl 12 жыл бұрын
nice..
@alvinlisyandi8541
@alvinlisyandi8541 3 жыл бұрын
Ilmunya sangat bermanfaat tapi sayang saya tidak bisa bahasa Inggris
@miklaszjay
@miklaszjay 7 жыл бұрын
Looks like he knows what he's doing, but his data sheet is kinda sketchy. He's going to run into trouble when he's making the plot...
@fss1704
@fss1704 6 жыл бұрын
miklaszj non automatic spectrometer in 2018? You've gotta be kidding me, my DIY spectrometer cost $50 and does auto sampling directly to my cellphone screen, seriously, all of that user interaction and sample being completely taken in a different direction each time should make your results have a mount everest of error.
@ethicalrevolution3294
@ethicalrevolution3294 4 жыл бұрын
@@fss1704 What is your phone spectrometer like?
@sdlmalkapurbuldana2175
@sdlmalkapurbuldana2175 5 жыл бұрын
PLEASE SEND ME SAME PROCEDURE FOR WATER FLUORIDE
@brianvandenberg4467
@brianvandenberg4467 6 жыл бұрын
6:07 Organic biotags charging for fun
@rebeccalames2495
@rebeccalames2495 3 жыл бұрын
bakit pa kasi online class
@crazyexpriementknig543
@crazyexpriementknig543 6 жыл бұрын
Bisleri Water ke jitne bhi test hai Jaise ki Sulfate /Nitrate /iron/Hardness/Calcium etc. Sabhi test Hindi Language me bhejo. Please.
@mataralhammadi3268
@mataralhammadi3268 4 жыл бұрын
1:20 looks like the guy from Jurassic Park
@oldboy4782
@oldboy4782 6 жыл бұрын
I almost gonna sleep
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